Streptomyces phage Yara

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1N0F3|A0A2P1N0F3_9CAUD Uncharacterized protein OS=Streptomyces phage Yara OX=2126736 GN=88 PE=4 SV=1
MM1 pKa = 7.82TDD3 pKa = 3.22LPMDD7 pKa = 4.14VMALLHH13 pKa = 6.16LVRR16 pKa = 11.84EE17 pKa = 4.41SVEE20 pKa = 3.87VDD22 pKa = 2.91NTVAPGRR29 pKa = 11.84VVGPWLEE36 pKa = 3.93IFEE39 pKa = 5.28DD40 pKa = 4.81GEE42 pKa = 4.34DD43 pKa = 3.56EE44 pKa = 4.51EE45 pKa = 5.45GFPVCYY51 pKa = 9.94AYY53 pKa = 9.23WVRR56 pKa = 11.84YY57 pKa = 9.09NPSNVARR64 pKa = 11.84YY65 pKa = 10.8DD66 pKa = 3.45MVEE69 pKa = 3.93WDD71 pKa = 4.13GSDD74 pKa = 3.95PDD76 pKa = 5.61SMDD79 pKa = 4.41LSLKK83 pKa = 10.45YY84 pKa = 10.07LTDD87 pKa = 3.43NALL90 pKa = 3.38

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1N0A8|A0A2P1N0A8_9CAUD Uncharacterized protein OS=Streptomyces phage Yara OX=2126736 GN=53 PE=4 SV=1
MM1 pKa = 7.52SSGMLNRR8 pKa = 11.84GDD10 pKa = 4.34VIWLVGEE17 pKa = 4.36ILQADD22 pKa = 3.54IQIRR26 pKa = 11.84GGNKK30 pKa = 8.9DD31 pKa = 3.0RR32 pKa = 11.84YY33 pKa = 8.02VAGRR37 pKa = 11.84RR38 pKa = 11.84VALAATACRR47 pKa = 11.84LLRR50 pKa = 11.84IARR53 pKa = 11.84PGLDD57 pKa = 4.34RR58 pKa = 11.84YY59 pKa = 7.01MTWVSEE65 pKa = 4.18QVEE68 pKa = 4.45SRR70 pKa = 11.84GRR72 pKa = 11.84RR73 pKa = 11.84IGDD76 pKa = 3.56LEE78 pKa = 4.48EE79 pKa = 4.47PGAWIRR85 pKa = 11.84KK86 pKa = 8.49NPAA89 pKa = 2.77

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

20651

37

1514

196.7

21.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.893 ± 0.409

1.133 ± 0.135

6.716 ± 0.211

7.36 ± 0.437

3.07 ± 0.152

8.024 ± 0.25

2.073 ± 0.18

4.818 ± 0.19

5.065 ± 0.352

7.351 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.876 ± 0.205

3.433 ± 0.224

4.925 ± 0.219

3.608 ± 0.122

6.319 ± 0.306

5.67 ± 0.223

6.043 ± 0.407

6.871 ± 0.269

2.019 ± 0.115

2.731 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski