Georgenia subflava

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Bogoriellaceae; Georgenia

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N7EHI8|A0A6N7EHI8_9MICO HPr family phosphocarrier protein OS=Georgenia subflava OX=1622177 GN=GB881_12455 PE=4 SV=1
MM1 pKa = 7.51APAGKK6 pKa = 9.57SRR8 pKa = 11.84RR9 pKa = 11.84AMVFGATAAFALVLAGCAEE28 pKa = 4.24TEE30 pKa = 4.18DD31 pKa = 4.35PDD33 pKa = 5.02GGGDD37 pKa = 3.42ATGGTDD43 pKa = 3.07GGSSEE48 pKa = 4.89GITVGTTDD56 pKa = 4.52VITNLDD62 pKa = 3.61PAGSYY67 pKa = 11.31DD68 pKa = 3.46NGSFAVQNQVFPFIMNTPYY87 pKa = 10.86GSPDD91 pKa = 3.56VEE93 pKa = 3.89PDD95 pKa = 3.01IAEE98 pKa = 4.28SAEE101 pKa = 4.0FTAPTEE107 pKa = 4.05YY108 pKa = 9.96TVKK111 pKa = 10.56LKK113 pKa = 10.97EE114 pKa = 4.14GLTFANGNEE123 pKa = 4.04LTASDD128 pKa = 3.97VKK130 pKa = 10.5FTFDD134 pKa = 3.24RR135 pKa = 11.84QIAIADD141 pKa = 3.66PSGPSSLLYY150 pKa = 10.79NLDD153 pKa = 3.48SVEE156 pKa = 4.25VVDD159 pKa = 5.06DD160 pKa = 3.82LTVVFHH166 pKa = 6.82LKK168 pKa = 10.4SEE170 pKa = 4.12NDD172 pKa = 3.26QVFPQILSSPAGPIVDD188 pKa = 3.91EE189 pKa = 5.9DD190 pKa = 4.14VFLPDD195 pKa = 4.21AVTPADD201 pKa = 5.13DD202 pKa = 3.78IVSGNAFAGQYY213 pKa = 10.81AITDD217 pKa = 3.58YY218 pKa = 11.27TEE220 pKa = 4.27NEE222 pKa = 4.39LIQYY226 pKa = 8.18QAFDD230 pKa = 4.31GYY232 pKa = 11.39DD233 pKa = 3.38GVLGAARR240 pKa = 11.84TDD242 pKa = 3.5TVTAQYY248 pKa = 10.32YY249 pKa = 10.56AEE251 pKa = 4.09EE252 pKa = 4.38TSLKK256 pKa = 10.64LAVQEE261 pKa = 4.16GDD263 pKa = 2.62IDD265 pKa = 3.88VAFRR269 pKa = 11.84SLSPTDD275 pKa = 3.79LADD278 pKa = 3.57LRR280 pKa = 11.84EE281 pKa = 4.47DD282 pKa = 3.82EE283 pKa = 4.77NVTVHH288 pKa = 7.11DD289 pKa = 4.85GPGGEE294 pKa = 3.61IRR296 pKa = 11.84YY297 pKa = 9.33IVFNFNTQPFGAEE310 pKa = 3.58TVEE313 pKa = 3.98ADD315 pKa = 3.43EE316 pKa = 4.89AKK318 pKa = 10.78ALAVRR323 pKa = 11.84QAVAHH328 pKa = 5.86SLDD331 pKa = 3.7RR332 pKa = 11.84DD333 pKa = 3.63ALSNEE338 pKa = 4.07IYY340 pKa = 10.54NGSFTPLYY348 pKa = 10.3SYY350 pKa = 11.07VPEE353 pKa = 4.54GLTGAVEE360 pKa = 4.14PLKK363 pKa = 10.6EE364 pKa = 4.24LYY366 pKa = 10.67GDD368 pKa = 3.93GAGGPDD374 pKa = 3.76ADD376 pKa = 3.77AAAAVLEE383 pKa = 4.37EE384 pKa = 4.87AGVEE388 pKa = 4.27TPVALNLQFSPDD400 pKa = 3.3HH401 pKa = 6.4YY402 pKa = 11.27GNSSDD407 pKa = 5.57LEE409 pKa = 4.14YY410 pKa = 11.46ALIEE414 pKa = 4.37SQLEE418 pKa = 3.88AGGLFDD424 pKa = 3.72VTLNSSLWDD433 pKa = 3.66AYY435 pKa = 9.75ATEE438 pKa = 4.71RR439 pKa = 11.84LTDD442 pKa = 3.59YY443 pKa = 10.91PSYY446 pKa = 10.34QLGWFPDD453 pKa = 3.67YY454 pKa = 11.48SDD456 pKa = 4.11ADD458 pKa = 3.57NYY460 pKa = 8.94LTPFFLIEE468 pKa = 3.99NFLGNGYY475 pKa = 10.87ANQEE479 pKa = 4.15VNDD482 pKa = 5.25LILDD486 pKa = 3.75QATTADD492 pKa = 3.68PDD494 pKa = 3.34EE495 pKa = 4.52RR496 pKa = 11.84AGKK499 pKa = 9.79IEE501 pKa = 4.0QIQEE505 pKa = 3.92LVAQDD510 pKa = 4.58LSTVPYY516 pKa = 10.3LQGAQVAVAATDD528 pKa = 3.35ITGVTDD534 pKa = 3.38TLDD537 pKa = 3.32ASFKK541 pKa = 10.4FRR543 pKa = 11.84YY544 pKa = 9.01AALGRR549 pKa = 3.94

Molecular weight: 58.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N7EQX2|A0A6N7EQX2_9MICO Alpha/beta fold hydrolase OS=Georgenia subflava OX=1622177 GN=GB881_18100 PE=3 SV=1
NN1 pKa = 7.57RR2 pKa = 11.84PPRR5 pKa = 11.84PRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84GRR11 pKa = 11.84GRR13 pKa = 11.84RR14 pKa = 11.84PRR16 pKa = 11.84GARR19 pKa = 11.84PRR21 pKa = 11.84RR22 pKa = 11.84PARR25 pKa = 11.84RR26 pKa = 11.84SPAGAAPTSSRR37 pKa = 11.84PHH39 pKa = 6.57APRR42 pKa = 11.84PLTSRR47 pKa = 11.84SGTVAEE53 pKa = 4.53PRR55 pKa = 11.84LTARR59 pKa = 11.84GG60 pKa = 3.52

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3778

0

3778

1231966

32

2007

326.1

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.927 ± 0.057

0.532 ± 0.008

6.357 ± 0.032

5.91 ± 0.038

2.567 ± 0.022

9.475 ± 0.041

2.176 ± 0.019

3.227 ± 0.025

1.485 ± 0.025

10.326 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.798 ± 0.015

1.639 ± 0.019

5.749 ± 0.036

2.65 ± 0.021

7.769 ± 0.044

4.967 ± 0.025

6.368 ± 0.031

9.73 ± 0.042

1.47 ± 0.018

1.879 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski