Cellulophaga phage phi17:1
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9ZYC3|R9ZYC3_9CAUD Phage minor capsid protein OS=Cellulophaga phage phi17:1 OX=1327980 GN=Phi17:1_gp38 PE=4 SV=1
MM1 pKa = 7.51 GMYY4 pKa = 9.89 TEE6 pKa = 5.88 LIFGAEE12 pKa = 4.42 LKK14 pKa = 10.37 QDD16 pKa = 3.35 TPEE19 pKa = 3.94 NVIEE23 pKa = 4.12 SLKK26 pKa = 11.09 YY27 pKa = 9.45 MMGDD31 pKa = 3.19 VEE33 pKa = 4.65 EE34 pKa = 4.82 KK35 pKa = 10.62 PEE37 pKa = 4.29 NFPLPDD43 pKa = 4.36 GRR45 pKa = 11.84 CEE47 pKa = 3.91 WLFKK51 pKa = 10.59 GSSYY55 pKa = 11.3 YY56 pKa = 10.77 FGINRR61 pKa = 11.84 AASSMWFDD69 pKa = 5.7 DD70 pKa = 4.39 ISEE73 pKa = 4.17 SWSISTRR80 pKa = 11.84 SNIKK84 pKa = 10.44 NYY86 pKa = 10.26 GDD88 pKa = 3.96 EE89 pKa = 4.22 IEE91 pKa = 6.13 SFLEE95 pKa = 4.35 WIKK98 pKa = 10.7 PYY100 pKa = 9.92 IDD102 pKa = 3.39 SGRR105 pKa = 11.84 GCRR108 pKa = 11.84 DD109 pKa = 2.71 MYY111 pKa = 11.32 AIVTYY116 pKa = 10.17 EE117 pKa = 3.9 EE118 pKa = 4.97 SDD120 pKa = 3.62 TPDD123 pKa = 2.68 IYY125 pKa = 11.6 YY126 pKa = 10.84 LSS128 pKa = 3.81
Molecular weight: 14.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.913
IPC2_protein 4.202
IPC_protein 4.113
Toseland 3.935
ProMoST 4.228
Dawson 4.062
Bjellqvist 4.228
Wikipedia 3.948
Rodwell 3.948
Grimsley 3.846
Solomon 4.062
Lehninger 4.012
Nozaki 4.177
DTASelect 4.329
Thurlkill 3.961
EMBOSS 3.961
Sillero 4.228
Patrickios 1.939
IPC_peptide 4.062
IPC2_peptide 4.215
IPC2.peptide.svr19 4.122
Protein with the highest isoelectric point:
>tr|R9ZYB1|R9ZYB1_9CAUD Uncharacterized protein OS=Cellulophaga phage phi17:1 OX=1327980 GN=Phi17:1_gp23 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.21 ISEE5 pKa = 4.33 TKK7 pKa = 10.05 KK8 pKa = 10.49 RR9 pKa = 11.84 EE10 pKa = 4.0 GVYY13 pKa = 10.45 CCAYY17 pKa = 10.03 GCKK20 pKa = 9.69 NDD22 pKa = 3.55 PDD24 pKa = 3.64 QRR26 pKa = 11.84 KK27 pKa = 9.65 GMLCHH32 pKa = 6.12 KK33 pKa = 10.03 HH34 pKa = 4.25 YY35 pKa = 11.23 ARR37 pKa = 11.84 LLRR40 pKa = 11.84 EE41 pKa = 4.02 RR42 pKa = 11.84 SPKK45 pKa = 9.31 KK46 pKa = 10.38 VRR48 pKa = 11.84 YY49 pKa = 9.44 SQMKK53 pKa = 9.36 QKK55 pKa = 10.63 AKK57 pKa = 10.56 SRR59 pKa = 11.84 GISFTITLEE68 pKa = 3.98 WFLKK72 pKa = 9.15 FCGRR76 pKa = 11.84 TGYY79 pKa = 9.11 MSKK82 pKa = 10.51 GRR84 pKa = 11.84 RR85 pKa = 11.84 GQNATLDD92 pKa = 3.82 RR93 pKa = 11.84 RR94 pKa = 11.84 CNLHH98 pKa = 7.13 GYY100 pKa = 9.53 HH101 pKa = 6.3 SWNIQILTNRR111 pKa = 11.84 QNASKK116 pKa = 10.71 GNRR119 pKa = 11.84 PSGEE123 pKa = 4.65 DD124 pKa = 3.39 FDD126 pKa = 6.13 CPFF129 pKa = 4.86
Molecular weight: 15.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.244
IPC2_protein 9.414
IPC_protein 9.458
Toseland 10.204
ProMoST 9.823
Dawson 10.365
Bjellqvist 10.043
Wikipedia 10.511
Rodwell 10.833
Grimsley 10.409
Solomon 10.394
Lehninger 10.365
Nozaki 10.262
DTASelect 10.014
Thurlkill 10.233
EMBOSS 10.599
Sillero 10.292
Patrickios 10.526
IPC_peptide 10.394
IPC2_peptide 9.048
IPC2.peptide.svr19 8.273
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
12369
40
905
190.3
21.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.072 ± 0.455
1.132 ± 0.131
6.589 ± 0.196
6.419 ± 0.328
4.738 ± 0.259
6.435 ± 0.522
1.366 ± 0.138
8.222 ± 0.274
8.869 ± 0.536
7.955 ± 0.305
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.66 ± 0.212
5.87 ± 0.318
3.412 ± 0.213
3.331 ± 0.203
3.735 ± 0.225
6.904 ± 0.331
5.441 ± 0.378
5.425 ± 0.191
1.334 ± 0.133
4.091 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here