Arthrobacter sp. Bz4
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2959 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T7TI53|A0A2T7TI53_9MICC Alcohol dehydrogenase OS=Arthrobacter sp. Bz4 OX=2171979 GN=DDA93_12895 PE=4 SV=1
MM1 pKa = 7.27 GWHH4 pKa = 6.75 TGPLAASLAAIAVAMSACGAVGVPASAPTGSSPADD39 pKa = 3.19 PVVPDD44 pKa = 4.85 PSPSSGIAATGEE56 pKa = 4.09 VLAQTTVWQQGDD68 pKa = 4.34 GLPQLCLGAVGEE80 pKa = 5.05 SYY82 pKa = 10.46 PPHH85 pKa = 6.83 CGGPVDD91 pKa = 5.12 LLNWDD96 pKa = 3.39 WDD98 pKa = 3.59 AVEE101 pKa = 4.19 QFEE104 pKa = 4.5 TASGVTWGTYY114 pKa = 9.18 AVQGTWDD121 pKa = 3.38 GKK123 pKa = 10.93 AFTRR127 pKa = 11.84 TQEE130 pKa = 4.44 PIPLSLYY137 pKa = 10.86 DD138 pKa = 4.95 PIADD142 pKa = 3.95 FDD144 pKa = 4.29 PRR146 pKa = 11.84 LDD148 pKa = 3.78 PANPGSNDD156 pKa = 2.96 EE157 pKa = 4.32 ATLEE161 pKa = 4.52 GIEE164 pKa = 4.12 LDD166 pKa = 3.56 IRR168 pKa = 11.84 ASGDD172 pKa = 3.27 PVIYY176 pKa = 10.6 SSITNGYY183 pKa = 9.15 LVVAVDD189 pKa = 3.83 YY190 pKa = 11.39 DD191 pKa = 3.65 DD192 pKa = 3.91 GTRR195 pKa = 11.84 QEE197 pKa = 4.23 RR198 pKa = 11.84 YY199 pKa = 9.93 DD200 pKa = 3.87 RR201 pKa = 11.84 EE202 pKa = 4.2 FGPDD206 pKa = 3.19 TVIVQSALRR215 pKa = 11.84 PVPP218 pKa = 3.53
Molecular weight: 22.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.579
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.884
Patrickios 1.036
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|A0A2T7TJK4|A0A2T7TJK4_9MICC Type I restriction enzyme R Protein OS=Arthrobacter sp. Bz4 OX=2171979 GN=DDA93_10690 PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2959
0
2959
946945
24
2270
320.0
34.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.31 ± 0.053
0.6 ± 0.012
5.946 ± 0.038
5.686 ± 0.04
3.191 ± 0.029
8.782 ± 0.04
2.109 ± 0.022
4.617 ± 0.033
2.453 ± 0.03
10.438 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.965 ± 0.018
2.439 ± 0.022
5.344 ± 0.034
3.257 ± 0.021
6.7 ± 0.038
6.141 ± 0.035
6.121 ± 0.03
8.442 ± 0.037
1.419 ± 0.016
2.039 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here