Millipede associated circular virus 1
Average proteome isoelectric point is 8.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPC9|A0A346BPC9_9VIRU Putative capsid protein OS=Millipede associated circular virus 1 OX=2293296 PE=4 SV=1
MM1 pKa = 7.78 PKK3 pKa = 10.65 AKK5 pKa = 9.8 FWCFTSFKK13 pKa = 10.44 DD14 pKa = 3.41 EE15 pKa = 4.42 HH16 pKa = 7.03 PNFCEE21 pKa = 3.99 EE22 pKa = 4.04 TFSYY26 pKa = 8.95 ITYY29 pKa = 9.76 QRR31 pKa = 11.84 EE32 pKa = 3.89 RR33 pKa = 11.84 CPTSSRR39 pKa = 11.84 EE40 pKa = 3.58 HH41 pKa = 4.65 WQGYY45 pKa = 6.87 VEE47 pKa = 4.85 CKK49 pKa = 9.72 RR50 pKa = 11.84 SSGVRR55 pKa = 11.84 TVKK58 pKa = 10.83 DD59 pKa = 3.51 GLGDD63 pKa = 3.79 NACHH67 pKa = 7.04 LEE69 pKa = 4.09 PSRR72 pKa = 11.84 STATWFKK79 pKa = 8.79 MARR82 pKa = 11.84 IATLSKK88 pKa = 10.05 SVRR91 pKa = 11.84 RR92 pKa = 11.84 GQRR95 pKa = 11.84 TDD97 pKa = 3.12 LEE99 pKa = 4.34 RR100 pKa = 11.84 VAKK103 pKa = 9.99 RR104 pKa = 11.84 VRR106 pKa = 11.84 DD107 pKa = 3.79 GGNLAEE113 pKa = 5.03 IATEE117 pKa = 4.45 FPVEE121 pKa = 4.12 TIKK124 pKa = 10.75 FGRR127 pKa = 11.84 GIQQLIEE134 pKa = 4.25 LNRR137 pKa = 11.84 KK138 pKa = 8.56 GRR140 pKa = 11.84 DD141 pKa = 3.26 GTIEE145 pKa = 4.13 AKK147 pKa = 10.73 VIIYY151 pKa = 8.58 WGKK154 pKa = 7.92 TGTGKK159 pKa = 8.02 TRR161 pKa = 11.84 RR162 pKa = 11.84 AFEE165 pKa = 4.34 RR166 pKa = 11.84 FPTAYY171 pKa = 10.03 FKK173 pKa = 10.8 PDD175 pKa = 3.26 GKK177 pKa = 10.12 WFDD180 pKa = 3.66 NYY182 pKa = 10.89 NGEE185 pKa = 4.44 DD186 pKa = 3.62 VIIFDD191 pKa = 5.39 DD192 pKa = 4.38 FNGHH196 pKa = 6.21 KK197 pKa = 10.29 DD198 pKa = 3.31 INVGMLLRR206 pKa = 11.84 ICDD209 pKa = 4.43 RR210 pKa = 11.84 YY211 pKa = 10.72 PMTVEE216 pKa = 4.93 KK217 pKa = 10.6 KK218 pKa = 10.78 GSSCKK223 pKa = 10.52 LNATTFVFTSNLHH236 pKa = 4.46 WKK238 pKa = 8.57 EE239 pKa = 3.76 WYY241 pKa = 9.89 NIEE244 pKa = 5.25 GMRR247 pKa = 11.84 QDD249 pKa = 5.31 WIAHH253 pKa = 5.34 WDD255 pKa = 3.3 AFEE258 pKa = 4.14 RR259 pKa = 11.84 RR260 pKa = 11.84 ITEE263 pKa = 3.56 IEE265 pKa = 3.91 AFDD268 pKa = 3.74 AVEE271 pKa = 4.87 PIDD274 pKa = 5.15 NILIMM279 pKa = 4.75
Molecular weight: 32.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.483
IPC2_protein 7.819
IPC_protein 7.717
Toseland 7.395
ProMoST 8.244
Dawson 8.434
Bjellqvist 8.756
Wikipedia 8.361
Rodwell 8.463
Grimsley 7.38
Solomon 8.551
Lehninger 8.565
Nozaki 8.99
DTASelect 8.492
Thurlkill 8.565
EMBOSS 8.653
Sillero 8.887
Patrickios 4.673
IPC_peptide 8.551
IPC2_peptide 7.644
IPC2.peptide.svr19 7.792
Protein with the highest isoelectric point:
>tr|A0A346BPC9|A0A346BPC9_9VIRU Putative capsid protein OS=Millipede associated circular virus 1 OX=2293296 PE=4 SV=1
MM1 pKa = 7.54 RR2 pKa = 11.84 RR3 pKa = 11.84 YY4 pKa = 9.48 SKK6 pKa = 10.53 KK7 pKa = 9.53 RR8 pKa = 11.84 SYY10 pKa = 10.92 RR11 pKa = 11.84 SASRR15 pKa = 11.84 SRR17 pKa = 11.84 RR18 pKa = 11.84 FRR20 pKa = 11.84 KK21 pKa = 8.77 GVRR24 pKa = 11.84 RR25 pKa = 11.84 TRR27 pKa = 11.84 VRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 IRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 SSKK39 pKa = 9.79 GNYY42 pKa = 8.16 FRR44 pKa = 11.84 RR45 pKa = 11.84 TFVDD49 pKa = 3.19 VGSIIGTLGDD59 pKa = 4.76 DD60 pKa = 4.01 GTFSPLTPNSDD71 pKa = 3.24 GTFTINTPGTFSIYY85 pKa = 10.91 VKK87 pKa = 8.13 TTILDD92 pKa = 4.31 SIPTASSYY100 pKa = 11.27 LLEE103 pKa = 4.27 FEE105 pKa = 4.73 AYY107 pKa = 9.65 RR108 pKa = 11.84 LRR110 pKa = 11.84 WCKK113 pKa = 10.33 LRR115 pKa = 11.84 VRR117 pKa = 11.84 NVTAQYY123 pKa = 11.17 LVATNSGDD131 pKa = 4.34 DD132 pKa = 3.63 VTPKK136 pKa = 10.24 QGSVYY141 pKa = 10.12 YY142 pKa = 10.98 VPDD145 pKa = 3.61 KK146 pKa = 11.21 YY147 pKa = 10.72 RR148 pKa = 11.84 IPSFSASSNYY158 pKa = 9.72 IKK160 pKa = 10.63 DD161 pKa = 3.1 ILQYY165 pKa = 9.12 MPKK168 pKa = 10.04 FAHH171 pKa = 6.0 QGQNFYY177 pKa = 11.22 CFNRR181 pKa = 11.84 NPKK184 pKa = 9.76 YY185 pKa = 9.36 LTPTISSDD193 pKa = 3.81 SIGGNISQYY202 pKa = 11.15 NSNLSCDD209 pKa = 3.92 WIRR212 pKa = 11.84 AQTTSGTQGTFPYY225 pKa = 9.98 YY226 pKa = 10.1 NLMWMVVDD234 pKa = 5.32 SPASAQMQFRR244 pKa = 11.84 FEE246 pKa = 5.73 LEE248 pKa = 4.37 TNWEE252 pKa = 4.38 FKK254 pKa = 10.47 GRR256 pKa = 11.84 PHH258 pKa = 7.44 GSYY261 pKa = 10.95 AA262 pKa = 3.46
Molecular weight: 30.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.926
IPC_protein 10.628
Toseland 10.262
ProMoST 10.116
Dawson 10.511
Bjellqvist 10.277
Wikipedia 10.76
Rodwell 10.687
Grimsley 10.613
Solomon 10.555
Lehninger 10.511
Nozaki 10.262
DTASelect 10.277
Thurlkill 10.35
EMBOSS 10.687
Sillero 10.438
Patrickios 10.087
IPC_peptide 10.555
IPC2_peptide 9.297
IPC2.peptide.svr19 8.393
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
541
262
279
270.5
31.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.991 ± 0.498
1.848 ± 0.442
5.36 ± 0.49
4.991 ± 1.937
6.1 ± 0.235
6.839 ± 0.22
1.479 ± 0.45
6.285 ± 0.592
5.915 ± 0.839
4.621 ± 0.214
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.033 ± 0.078
4.806 ± 0.338
4.067 ± 0.563
2.957 ± 0.54
9.797 ± 0.799
7.948 ± 2.201
7.948 ± 0.282
4.806 ± 0.098
2.403 ± 0.551
4.806 ± 1.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here