Amino acid dipepetide frequency for Escherichia phage lambda (Bacteriophage lambda)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.069AlaAla: 14.069 ± 3.13
0.546AlaCys: 0.546 ± 0.217
5.054AlaAsp: 5.054 ± 0.545
7.171AlaGlu: 7.171 ± 0.826
4.166AlaPhe: 4.166 ± 0.613
7.308AlaGly: 7.308 ± 0.964
1.844AlaHis: 1.844 ± 0.573
5.532AlaIle: 5.532 ± 0.595
4.644AlaLys: 4.644 ± 0.753
8.196AlaLeu: 8.196 ± 0.854
2.937AlaMet: 2.937 ± 0.409
2.664AlaAsn: 2.664 ± 0.497
2.459AlaPro: 2.459 ± 0.448
3.893AlaGln: 3.893 ± 0.87
6.83AlaArg: 6.83 ± 0.867
8.264AlaSer: 8.264 ± 1.447
4.917AlaThr: 4.917 ± 1.037
6.693AlaVal: 6.693 ± 0.808
1.776AlaTrp: 1.776 ± 0.377
3.073AlaTyr: 3.073 ± 0.43
0.0AlaXaa: 0.0 ± 0.0
Cys
0.888CysAla: 0.888 ± 0.296
0.478CysCys: 0.478 ± 0.213
0.478CysAsp: 0.478 ± 0.174
0.751CysGlu: 0.751 ± 0.248
0.205CysPhe: 0.205 ± 0.121
0.956CysGly: 0.956 ± 0.275
0.273CysHis: 0.273 ± 0.178
0.956CysIle: 0.956 ± 0.289
0.615CysLys: 0.615 ± 0.234
0.615CysLeu: 0.615 ± 0.164
0.273CysMet: 0.273 ± 0.145
0.615CysAsn: 0.615 ± 0.179
0.41CysPro: 0.41 ± 0.179
0.205CysGln: 0.205 ± 0.128
1.161CysArg: 1.161 ± 0.348
1.093CysSer: 1.093 ± 0.296
0.956CysThr: 0.956 ± 0.242
0.683CysVal: 0.683 ± 0.222
0.205CysTrp: 0.205 ± 0.12
0.615CysTyr: 0.615 ± 0.176
0.0CysXaa: 0.0 ± 0.0
Asp
5.942AspAla: 5.942 ± 0.812
0.546AspCys: 0.546 ± 0.176
4.03AspAsp: 4.03 ± 0.492
4.508AspGlu: 4.508 ± 0.622
1.844AspPhe: 1.844 ± 0.283
5.191AspGly: 5.191 ± 0.637
0.615AspHis: 0.615 ± 0.201
4.371AspIle: 4.371 ± 0.685
3.073AspLys: 3.073 ± 0.456
3.961AspLeu: 3.961 ± 0.63
1.844AspMet: 1.844 ± 0.406
1.776AspAsn: 1.776 ± 0.297
2.459AspPro: 2.459 ± 0.609
0.888AspGln: 0.888 ± 0.24
2.39AspArg: 2.39 ± 0.479
3.142AspSer: 3.142 ± 0.452
3.347AspThr: 3.347 ± 0.479
3.893AspVal: 3.893 ± 0.576
1.161AspTrp: 1.161 ± 0.295
1.981AspTyr: 1.981 ± 0.384
0.0AspXaa: 0.0 ± 0.0
Glu
6.625GluAla: 6.625 ± 0.901
0.956GluCys: 0.956 ± 0.31
3.415GluAsp: 3.415 ± 0.456
4.371GluGlu: 4.371 ± 0.553
2.39GluPhe: 2.39 ± 0.436
3.278GluGly: 3.278 ± 0.504
1.229GluHis: 1.229 ± 0.358
3.347GluIle: 3.347 ± 0.484
3.825GluLys: 3.825 ± 0.451
6.215GluLeu: 6.215 ± 0.732
1.707GluMet: 1.707 ± 0.381
3.073GluAsn: 3.073 ± 0.462
1.776GluPro: 1.776 ± 0.382
5.122GluGln: 5.122 ± 0.647
4.234GluArg: 4.234 ± 0.688
3.961GluSer: 3.961 ± 0.48
3.483GluThr: 3.483 ± 0.492
3.62GluVal: 3.62 ± 0.58
1.024GluTrp: 1.024 ± 0.266
1.844GluTyr: 1.844 ± 0.333
0.0GluXaa: 0.0 ± 0.0
Phe
2.868PheAla: 2.868 ± 0.726
0.888PheCys: 0.888 ± 0.229
2.8PheAsp: 2.8 ± 0.407
1.707PheGlu: 1.707 ± 0.324
1.161PhePhe: 1.161 ± 0.277
2.254PheGly: 2.254 ± 0.384
1.161PheHis: 1.161 ± 0.268
1.981PheIle: 1.981 ± 0.445
1.229PheLys: 1.229 ± 0.262
2.664PheLeu: 2.664 ± 0.439
0.683PheMet: 0.683 ± 0.205
1.024PheAsn: 1.024 ± 0.218
1.503PhePro: 1.503 ± 0.358
0.615PheGln: 0.615 ± 0.194
2.732PheArg: 2.732 ± 0.388
3.142PheSer: 3.142 ± 0.592
2.8PheThr: 2.8 ± 0.489
2.664PheVal: 2.664 ± 0.461
0.546PheTrp: 0.546 ± 0.18
0.82PheTyr: 0.82 ± 0.277
0.0PheXaa: 0.0 ± 0.0
Gly
5.532GlyAla: 5.532 ± 0.783
0.683GlyCys: 0.683 ± 0.236
4.166GlyAsp: 4.166 ± 0.558
3.756GlyGlu: 3.756 ± 0.518
1.981GlyPhe: 1.981 ± 0.37
4.712GlyGly: 4.712 ± 0.826
0.956GlyHis: 0.956 ± 0.273
3.893GlyIle: 3.893 ± 0.46
4.098GlyLys: 4.098 ± 0.465
4.576GlyLeu: 4.576 ± 0.554
2.664GlyMet: 2.664 ± 0.451
3.278GlyAsn: 3.278 ± 0.441
1.093GlyPro: 1.093 ± 0.221
3.278GlyGln: 3.278 ± 0.473
4.712GlyArg: 4.712 ± 0.483
3.961GlySer: 3.961 ± 0.518
4.849GlyThr: 4.849 ± 0.804
4.849GlyVal: 4.849 ± 0.547
1.503GlyTrp: 1.503 ± 0.281
2.732GlyTyr: 2.732 ± 0.484
0.0GlyXaa: 0.0 ± 0.0
His
1.366HisAla: 1.366 ± 0.336
0.205HisCys: 0.205 ± 0.107
0.751HisAsp: 0.751 ± 0.219
0.82HisGlu: 0.82 ± 0.311
1.024HisPhe: 1.024 ± 0.324
1.229HisGly: 1.229 ± 0.288
0.41HisHis: 0.41 ± 0.148
1.093HisIle: 1.093 ± 0.254
1.229HisLys: 1.229 ± 0.343
1.707HisLeu: 1.707 ± 0.325
0.273HisMet: 0.273 ± 0.136
0.751HisAsn: 0.751 ± 0.215
1.298HisPro: 1.298 ± 0.307
0.546HisGln: 0.546 ± 0.173
1.229HisArg: 1.229 ± 0.265
1.093HisSer: 1.093 ± 0.341
1.024HisThr: 1.024 ± 0.271
1.024HisVal: 1.024 ± 0.262
0.068HisTrp: 0.068 ± 0.074
1.024HisTyr: 1.024 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
5.191IleAla: 5.191 ± 0.678
0.683IleCys: 0.683 ± 0.223
3.415IleAsp: 3.415 ± 0.576
3.756IleGlu: 3.756 ± 0.617
1.024IlePhe: 1.024 ± 0.271
3.756IleGly: 3.756 ± 0.67
0.956IleHis: 0.956 ± 0.242
2.322IleIle: 2.322 ± 0.498
3.278IleLys: 3.278 ± 0.553
3.415IleLeu: 3.415 ± 0.412
0.888IleMet: 0.888 ± 0.258
2.664IleAsn: 2.664 ± 0.406
2.595IlePro: 2.595 ± 0.399
2.049IleGln: 2.049 ± 0.375
3.278IleArg: 3.278 ± 0.47
3.825IleSer: 3.825 ± 0.48
4.166IleThr: 4.166 ± 0.725
2.868IleVal: 2.868 ± 0.355
0.751IleTrp: 0.751 ± 0.28
1.229IleTyr: 1.229 ± 0.357
0.0IleXaa: 0.0 ± 0.0
Lys
5.054LysAla: 5.054 ± 0.753
0.82LysCys: 0.82 ± 0.279
3.073LysAsp: 3.073 ± 0.54
3.62LysGlu: 3.62 ± 0.505
1.571LysPhe: 1.571 ± 0.342
2.732LysGly: 2.732 ± 0.431
1.434LysHis: 1.434 ± 0.326
2.254LysIle: 2.254 ± 0.405
3.483LysLys: 3.483 ± 0.695
4.166LysLeu: 4.166 ± 0.713
1.161LysMet: 1.161 ± 0.303
2.664LysAsn: 2.664 ± 0.574
3.142LysPro: 3.142 ± 0.561
2.664LysGln: 2.664 ± 0.403
3.825LysArg: 3.825 ± 0.575
3.825LysSer: 3.825 ± 0.459
3.893LysThr: 3.893 ± 0.536
2.937LysVal: 2.937 ± 0.464
1.161LysTrp: 1.161 ± 0.291
1.776LysTyr: 1.776 ± 0.401
0.0LysXaa: 0.0 ± 0.0
Leu
8.674LeuAla: 8.674 ± 0.833
0.956LeuCys: 0.956 ± 0.25
3.483LeuAsp: 3.483 ± 0.465
4.234LeuGlu: 4.234 ± 0.538
2.527LeuPhe: 2.527 ± 0.46
4.098LeuGly: 4.098 ± 0.511
1.298LeuHis: 1.298 ± 0.348
3.278LeuIle: 3.278 ± 0.596
5.054LeuLys: 5.054 ± 0.703
5.805LeuLeu: 5.805 ± 0.683
1.776LeuMet: 1.776 ± 0.339
3.551LeuAsn: 3.551 ± 0.503
4.166LeuPro: 4.166 ± 0.437
2.937LeuGln: 2.937 ± 0.5
5.054LeuArg: 5.054 ± 0.54
7.239LeuSer: 7.239 ± 0.773
5.942LeuThr: 5.942 ± 0.687
4.576LeuVal: 4.576 ± 0.551
1.434LeuTrp: 1.434 ± 0.262
1.434LeuTyr: 1.434 ± 0.333
0.0LeuXaa: 0.0 ± 0.0
Met
3.005MetAla: 3.005 ± 0.624
0.273MetCys: 0.273 ± 0.131
1.298MetAsp: 1.298 ± 0.319
1.093MetGlu: 1.093 ± 0.269
0.888MetPhe: 0.888 ± 0.229
1.298MetGly: 1.298 ± 0.263
0.683MetHis: 0.683 ± 0.262
1.093MetIle: 1.093 ± 0.277
1.639MetLys: 1.639 ± 0.386
2.39MetLeu: 2.39 ± 0.363
0.956MetMet: 0.956 ± 0.247
1.024MetAsn: 1.024 ± 0.234
1.571MetPro: 1.571 ± 0.335
1.503MetGln: 1.503 ± 0.377
2.049MetArg: 2.049 ± 0.343
1.844MetSer: 1.844 ± 0.337
2.8MetThr: 2.8 ± 0.573
1.707MetVal: 1.707 ± 0.317
0.273MetTrp: 0.273 ± 0.12
0.956MetTyr: 0.956 ± 0.311
0.0MetXaa: 0.0 ± 0.0
Asn
3.961AsnAla: 3.961 ± 0.632
0.751AsnCys: 0.751 ± 0.22
1.981AsnAsp: 1.981 ± 0.311
3.142AsnGlu: 3.142 ± 0.459
1.093AsnPhe: 1.093 ± 0.346
3.756AsnGly: 3.756 ± 0.566
0.956AsnHis: 0.956 ± 0.278
1.981AsnIle: 1.981 ± 0.38
2.254AsnLys: 2.254 ± 0.481
2.595AsnLeu: 2.595 ± 0.449
0.956AsnMet: 0.956 ± 0.234
2.049AsnAsn: 2.049 ± 0.451
1.844AsnPro: 1.844 ± 0.306
1.571AsnGln: 1.571 ± 0.444
2.595AsnArg: 2.595 ± 0.572
2.868AsnSer: 2.868 ± 0.452
2.254AsnThr: 2.254 ± 0.486
1.981AsnVal: 1.981 ± 0.29
0.478AsnTrp: 0.478 ± 0.179
1.229AsnTyr: 1.229 ± 0.258
0.0AsnXaa: 0.0 ± 0.0
Pro
4.234ProAla: 4.234 ± 0.617
0.273ProCys: 0.273 ± 0.148
3.347ProAsp: 3.347 ± 0.542
2.732ProGlu: 2.732 ± 0.487
1.434ProPhe: 1.434 ± 0.328
3.005ProGly: 3.005 ± 0.395
0.683ProHis: 0.683 ± 0.208
1.503ProIle: 1.503 ± 0.392
2.322ProLys: 2.322 ± 0.481
3.278ProLeu: 3.278 ± 0.496
0.82ProMet: 0.82 ± 0.185
1.366ProAsn: 1.366 ± 0.282
1.776ProPro: 1.776 ± 0.493
1.503ProGln: 1.503 ± 0.276
1.434ProArg: 1.434 ± 0.345
2.459ProSer: 2.459 ± 0.427
2.39ProThr: 2.39 ± 0.361
3.278ProVal: 3.278 ± 0.402
0.683ProTrp: 0.683 ± 0.221
1.093ProTyr: 1.093 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
4.098GlnAla: 4.098 ± 0.722
0.546GlnCys: 0.546 ± 0.162
1.366GlnAsp: 1.366 ± 0.268
3.005GlnGlu: 3.005 ± 0.458
1.366GlnPhe: 1.366 ± 0.27
1.844GlnGly: 1.844 ± 0.325
0.546GlnHis: 0.546 ± 0.186
2.732GlnIle: 2.732 ± 0.364
2.527GlnLys: 2.527 ± 0.446
3.142GlnLeu: 3.142 ± 0.432
1.434GlnMet: 1.434 ± 0.357
1.844GlnAsn: 1.844 ± 0.364
1.639GlnPro: 1.639 ± 0.322
2.459GlnGln: 2.459 ± 0.608
2.595GlnArg: 2.595 ± 0.438
3.073GlnSer: 3.073 ± 0.462
2.322GlnThr: 2.322 ± 0.465
3.278GlnVal: 3.278 ± 0.453
0.615GlnTrp: 0.615 ± 0.236
1.503GlnTyr: 1.503 ± 0.265
0.0GlnXaa: 0.0 ± 0.0
Arg
4.781ArgAla: 4.781 ± 0.627
0.888ArgCys: 0.888 ± 0.28
3.893ArgAsp: 3.893 ± 0.651
5.122ArgGlu: 5.122 ± 0.746
1.912ArgPhe: 1.912 ± 0.351
4.234ArgGly: 4.234 ± 0.57
1.229ArgHis: 1.229 ± 0.333
3.893ArgIle: 3.893 ± 0.606
3.21ArgLys: 3.21 ± 0.442
5.054ArgLeu: 5.054 ± 0.6
2.39ArgMet: 2.39 ± 0.36
3.005ArgAsn: 3.005 ± 0.437
1.434ArgPro: 1.434 ± 0.267
3.142ArgGln: 3.142 ± 0.511
5.464ArgArg: 5.464 ± 0.891
3.347ArgSer: 3.347 ± 0.484
3.21ArgThr: 3.21 ± 0.384
3.21ArgVal: 3.21 ± 0.546
1.366ArgTrp: 1.366 ± 0.338
2.254ArgTyr: 2.254 ± 0.335
0.0ArgXaa: 0.0 ± 0.0
Ser
8.674SerAla: 8.674 ± 1.844
0.615SerCys: 0.615 ± 0.194
4.439SerAsp: 4.439 ± 0.706
4.986SerGlu: 4.986 ± 0.597
2.595SerPhe: 2.595 ± 0.387
6.83SerGly: 6.83 ± 0.858
1.229SerHis: 1.229 ± 0.331
2.459SerIle: 2.459 ± 0.376
2.937SerLys: 2.937 ± 0.434
4.917SerLeu: 4.917 ± 0.764
2.39SerMet: 2.39 ± 0.384
2.049SerAsn: 2.049 ± 0.359
2.8SerPro: 2.8 ± 0.527
3.21SerGln: 3.21 ± 0.435
4.439SerArg: 4.439 ± 0.58
4.234SerSer: 4.234 ± 0.688
4.234SerThr: 4.234 ± 0.715
5.327SerVal: 5.327 ± 0.692
0.683SerTrp: 0.683 ± 0.268
1.366SerTyr: 1.366 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
7.444ThrAla: 7.444 ± 1.008
0.683ThrCys: 0.683 ± 0.198
3.347ThrAsp: 3.347 ± 0.438
3.961ThrGlu: 3.961 ± 0.69
3.005ThrPhe: 3.005 ± 0.517
4.234ThrGly: 4.234 ± 0.588
0.888ThrHis: 0.888 ± 0.261
3.005ThrIle: 3.005 ± 0.483
3.347ThrLys: 3.347 ± 0.456
5.327ThrLeu: 5.327 ± 0.66
1.503ThrMet: 1.503 ± 0.328
1.912ThrAsn: 1.912 ± 0.495
3.415ThrPro: 3.415 ± 0.59
2.185ThrGln: 2.185 ± 0.443
2.868ThrArg: 2.868 ± 0.427
4.03ThrSer: 4.03 ± 0.634
3.483ThrThr: 3.483 ± 0.458
4.849ThrVal: 4.849 ± 0.858
1.161ThrTrp: 1.161 ± 0.305
1.844ThrTyr: 1.844 ± 0.361
0.0ThrXaa: 0.0 ± 0.0
Val
6.01ValAla: 6.01 ± 0.766
0.683ValCys: 0.683 ± 0.248
3.62ValAsp: 3.62 ± 0.471
3.961ValGlu: 3.961 ± 0.466
3.073ValPhe: 3.073 ± 0.406
3.756ValGly: 3.756 ± 0.558
0.751ValHis: 0.751 ± 0.224
3.688ValIle: 3.688 ± 0.482
4.371ValLys: 4.371 ± 0.449
5.464ValLeu: 5.464 ± 0.741
2.459ValMet: 2.459 ± 0.466
3.142ValAsn: 3.142 ± 0.466
2.117ValPro: 2.117 ± 0.403
2.254ValGln: 2.254 ± 0.577
3.005ValArg: 3.005 ± 0.449
5.191ValSer: 5.191 ± 0.649
4.234ValThr: 4.234 ± 0.701
4.508ValVal: 4.508 ± 0.431
0.683ValTrp: 0.683 ± 0.205
1.912ValTyr: 1.912 ± 0.424
0.0ValXaa: 0.0 ± 0.0
Trp
1.229TrpAla: 1.229 ± 0.264
0.478TrpCys: 0.478 ± 0.164
1.093TrpAsp: 1.093 ± 0.273
0.546TrpGlu: 0.546 ± 0.195
0.546TrpPhe: 0.546 ± 0.207
0.751TrpGly: 0.751 ± 0.236
0.546TrpHis: 0.546 ± 0.217
0.82TrpIle: 0.82 ± 0.257
1.229TrpLys: 1.229 ± 0.309
1.434TrpLeu: 1.434 ± 0.332
0.478TrpMet: 0.478 ± 0.17
0.82TrpAsn: 0.82 ± 0.204
0.888TrpPro: 0.888 ± 0.241
0.478TrpGln: 0.478 ± 0.156
0.956TrpArg: 0.956 ± 0.265
1.161TrpSer: 1.161 ± 0.275
0.888TrpThr: 0.888 ± 0.262
1.229TrpVal: 1.229 ± 0.278
0.341TrpTrp: 0.341 ± 0.168
0.478TrpTyr: 0.478 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.39TyrAla: 2.39 ± 0.462
0.478TyrCys: 0.478 ± 0.166
1.912TyrAsp: 1.912 ± 0.406
2.254TyrGlu: 2.254 ± 0.501
1.434TyrPhe: 1.434 ± 0.283
2.049TyrGly: 2.049 ± 0.362
0.478TyrHis: 0.478 ± 0.136
1.912TyrIle: 1.912 ± 0.401
0.888TyrLys: 0.888 ± 0.286
2.595TyrLeu: 2.595 ± 0.451
0.683TyrMet: 0.683 ± 0.234
1.024TyrAsn: 1.024 ± 0.216
1.229TyrPro: 1.229 ± 0.299
1.434TyrGln: 1.434 ± 0.248
2.185TyrArg: 2.185 ± 0.4
2.664TyrSer: 2.664 ± 0.526
1.298TyrThr: 1.298 ± 0.288
1.776TyrVal: 1.776 ± 0.363
0.478TyrTrp: 0.478 ± 0.173
0.956TyrTyr: 0.956 ± 0.253
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (14643 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski