Cellulophaga phage phi10:1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZYF2|R9ZYF2_9CAUD Uncharacterized protein OS=Cellulophaga phage phi10:1 OX=1327981 GN=Phi10:1_gp013 PE=4 SV=1
MM1 pKa = 7.07KK2 pKa = 10.47QKK4 pKa = 9.76LTDD7 pKa = 3.79SIFNKK12 pKa = 10.48LIEE15 pKa = 4.59NKK17 pKa = 8.96EE18 pKa = 3.92QQHH21 pKa = 6.91DD22 pKa = 3.75YY23 pKa = 11.64DD24 pKa = 4.86FFAFDD29 pKa = 3.67FEE31 pKa = 4.28YY32 pKa = 10.86RR33 pKa = 11.84NYY35 pKa = 10.63SFSIQGYY42 pKa = 9.58KK43 pKa = 10.09SDD45 pKa = 4.37DD46 pKa = 3.62LEE48 pKa = 5.41LIISNDD54 pKa = 3.44YY55 pKa = 7.98YY56 pKa = 11.15TKK58 pKa = 10.78NDD60 pKa = 3.66CEE62 pKa = 4.76LSFTSNQKK70 pKa = 8.64ATLQALLVEE79 pKa = 4.55AQEE82 pKa = 4.4EE83 pKa = 4.63EE84 pKa = 4.4NNEE87 pKa = 3.83PVYY90 pKa = 10.86DD91 pKa = 4.16YY92 pKa = 11.65DD93 pKa = 4.72DD94 pKa = 5.76ADD96 pKa = 4.29DD97 pKa = 4.5ASEE100 pKa = 4.02FNRR103 pKa = 11.84NYY105 pKa = 10.46FDD107 pKa = 3.46

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A0T8|S0A0T8_9CAUD Phage tail protein OS=Cellulophaga phage phi10:1 OX=1327981 GN=Phi10:1_gp084 PE=4 SV=1
MM1 pKa = 7.89LEE3 pKa = 4.29VIFDD7 pKa = 4.6FYY9 pKa = 11.57DD10 pKa = 3.28VHH12 pKa = 9.15SKK14 pKa = 9.55IQRR17 pKa = 11.84KK18 pKa = 9.65KK19 pKa = 9.94GDD21 pKa = 3.23RR22 pKa = 11.84FRR24 pKa = 11.84PDD26 pKa = 2.52NGQRR30 pKa = 11.84RR31 pKa = 11.84LEE33 pKa = 4.03LLKK36 pKa = 10.99GGAVIQLRR44 pKa = 11.84KK45 pKa = 9.31YY46 pKa = 10.72RR47 pKa = 11.84KK48 pKa = 8.89DD49 pKa = 3.1DD50 pKa = 3.35TFRR53 pKa = 11.84SHH55 pKa = 7.18IVRR58 pKa = 4.09

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

16524

33

777

155.9

17.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.962 ± 0.392

1.259 ± 0.147

6.5 ± 0.222

7.462 ± 0.397

4.66 ± 0.207

5.604 ± 0.278

1.465 ± 0.123

7.958 ± 0.272

9.084 ± 0.493

8.781 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.158

6.79 ± 0.266

2.614 ± 0.167

2.808 ± 0.13

3.571 ± 0.18

6.651 ± 0.32

6.385 ± 0.361

5.616 ± 0.205

1.192 ± 0.09

4.454 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski