Sanxia sobemo-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEQ8|A0A1L3KEQ8_9VIRU RNA-directed RNA polymerase OS=Sanxia sobemo-like virus 2 OX=1923381 PE=4 SV=1
MM1 pKa = 7.44SGSNYY6 pKa = 9.58TFLKK10 pKa = 10.63SALQLTTLLLCVGTFVDD27 pKa = 4.17TFDD30 pKa = 4.67SEE32 pKa = 4.54EE33 pKa = 4.4NKK35 pKa = 10.63CQMDD39 pKa = 4.34LNHH42 pKa = 7.07ALAAKK47 pKa = 9.69TEE49 pKa = 4.2MEE51 pKa = 4.46HH52 pKa = 4.78EE53 pKa = 4.21QKK55 pKa = 11.16YY56 pKa = 10.54LDD58 pKa = 3.76SKK60 pKa = 10.61MRR62 pKa = 11.84ALMDD66 pKa = 3.34KK67 pKa = 10.1MVEE70 pKa = 4.01EE71 pKa = 4.38KK72 pKa = 10.89AAVLLADD79 pKa = 3.79KK80 pKa = 10.8QILEE84 pKa = 4.31LAHH87 pKa = 6.49NLTVLGKK94 pKa = 7.38EE95 pKa = 4.09HH96 pKa = 7.16KK97 pKa = 10.18ALKK100 pKa = 10.22SKK102 pKa = 10.94QNLVEE107 pKa = 4.27RR108 pKa = 11.84DD109 pKa = 3.69FLKK112 pKa = 10.35MSRR115 pKa = 11.84KK116 pKa = 8.31LTGEE120 pKa = 3.76RR121 pKa = 11.84SRR123 pKa = 11.84NKK125 pKa = 10.24RR126 pKa = 11.84SITTKK131 pKa = 9.44EE132 pKa = 3.72QQIGNMIGKK141 pKa = 9.43SKK143 pKa = 9.62SQRR146 pKa = 11.84DD147 pKa = 3.55EE148 pKa = 4.86LEE150 pKa = 3.73EE151 pKa = 4.13CEE153 pKa = 4.21RR154 pKa = 11.84RR155 pKa = 11.84VIALQSSVDD164 pKa = 3.61SLNYY168 pKa = 9.61WKK170 pKa = 10.63DD171 pKa = 3.47EE172 pKa = 3.85LRR174 pKa = 11.84KK175 pKa = 9.94KK176 pKa = 10.69VEE178 pKa = 3.75QLEE181 pKa = 4.43DD182 pKa = 3.94DD183 pKa = 4.26NEE185 pKa = 4.11DD186 pKa = 3.05LRR188 pKa = 11.84EE189 pKa = 3.91RR190 pKa = 11.84MKK192 pKa = 10.88TSASQIDD199 pKa = 3.75FRR201 pKa = 11.84MVGGLILLFATVVVSLAWYY220 pKa = 8.45KK221 pKa = 10.01HH222 pKa = 5.44RR223 pKa = 11.84KK224 pKa = 8.68VVTLLKK230 pKa = 10.79EE231 pKa = 4.23EE232 pKa = 4.94IITLQTRR239 pKa = 11.84VEE241 pKa = 4.58SMTQRR246 pKa = 11.84YY247 pKa = 9.35SPVQMLANSMTNVSLGNSSNEE268 pKa = 3.96SLIPEE273 pKa = 4.41CKK275 pKa = 10.46VPGNALFPHH284 pKa = 7.14DD285 pKa = 5.04SDD287 pKa = 4.46PGQLTILLKK296 pKa = 10.7IGDD299 pKa = 4.37ALVAAGQVFWIKK311 pKa = 10.88DD312 pKa = 3.45WLVTAAHH319 pKa = 6.82VINAVSEE326 pKa = 4.3DD327 pKa = 3.52EE328 pKa = 5.62DD329 pKa = 5.0IILSSMVPQADD340 pKa = 3.79GTRR343 pKa = 11.84KK344 pKa = 8.47EE345 pKa = 3.92VRR347 pKa = 11.84VNRR350 pKa = 11.84SRR352 pKa = 11.84KK353 pKa = 9.81DD354 pKa = 3.35FLIPTPSFDD363 pKa = 3.7LAYY366 pKa = 9.88MKK368 pKa = 10.6VDD370 pKa = 3.32NSFMQQFKK378 pKa = 11.01SNKK381 pKa = 8.97FKK383 pKa = 10.78TNHH386 pKa = 6.34LSSDD390 pKa = 3.92VYY392 pKa = 11.26VSVSAEE398 pKa = 3.64GMASTGHH405 pKa = 6.02MEE407 pKa = 4.36PNDD410 pKa = 4.49DD411 pKa = 4.93GSVTYY416 pKa = 10.58YY417 pKa = 11.31GSTTPGFSGAPYY429 pKa = 9.84LWDD432 pKa = 3.63GKK434 pKa = 10.11VVGMHH439 pKa = 6.48LGARR443 pKa = 11.84AKK445 pKa = 9.57EE446 pKa = 4.04VCQYY450 pKa = 10.33GLSIAIIEE458 pKa = 4.65RR459 pKa = 11.84ILTRR463 pKa = 11.84FSRR466 pKa = 11.84PYY468 pKa = 9.79KK469 pKa = 10.65AEE471 pKa = 3.89STEE474 pKa = 4.53DD475 pKa = 3.3EE476 pKa = 4.35LFEE479 pKa = 4.96KK480 pKa = 10.68NFYY483 pKa = 7.7EE484 pKa = 4.24TLEE487 pKa = 3.92EE488 pKa = 3.81RR489 pKa = 11.84AMVGGRR495 pKa = 11.84IKK497 pKa = 10.74AKK499 pKa = 10.18RR500 pKa = 11.84INPEE504 pKa = 3.91DD505 pKa = 3.34FVVYY509 pKa = 10.85YY510 pKa = 10.31KK511 pKa = 10.67DD512 pKa = 2.92KK513 pKa = 11.11HH514 pKa = 6.53GNHH517 pKa = 5.58QSGVISDD524 pKa = 4.09MDD526 pKa = 3.72IEE528 pKa = 6.68DD529 pKa = 4.32DD530 pKa = 5.47DD531 pKa = 4.27IYY533 pKa = 11.53NYY535 pKa = 11.0LDD537 pKa = 3.38FGGDD541 pKa = 3.31LEE543 pKa = 4.73EE544 pKa = 5.87ADD546 pKa = 3.82EE547 pKa = 4.78YY548 pKa = 11.0YY549 pKa = 10.93SKK551 pKa = 11.11RR552 pKa = 11.84NPKK555 pKa = 10.59DD556 pKa = 3.08SDD558 pKa = 3.23FRR560 pKa = 11.84KK561 pKa = 10.1RR562 pKa = 11.84KK563 pKa = 8.27GRR565 pKa = 11.84RR566 pKa = 11.84YY567 pKa = 10.15DD568 pKa = 3.27PEE570 pKa = 4.3VYY572 pKa = 10.34FDD574 pKa = 3.99VPAHH578 pKa = 6.0FPKK581 pKa = 10.51NSKK584 pKa = 10.08KK585 pKa = 8.76EE586 pKa = 3.55RR587 pKa = 11.84SMISRR592 pKa = 11.84EE593 pKa = 3.84MGKK596 pKa = 10.35KK597 pKa = 8.87IVEE600 pKa = 4.25VQTEE604 pKa = 4.0KK605 pKa = 11.05DD606 pKa = 3.43VEE608 pKa = 4.39EE609 pKa = 4.41NGKK612 pKa = 9.39IDD614 pKa = 4.16PAGDD618 pKa = 3.5AQKK621 pKa = 11.09QSIQKK626 pKa = 10.44SNDD629 pKa = 2.93PMISILAAMTKK640 pKa = 9.97ISDD643 pKa = 3.89GQEE646 pKa = 3.65SMLALLKK653 pKa = 10.48RR654 pKa = 11.84ALPSTSSGSKK664 pKa = 9.75RR665 pKa = 11.84GRR667 pKa = 11.84QSRR670 pKa = 11.84SPMSQNKK677 pKa = 9.05

Molecular weight: 76.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEQ8|A0A1L3KEQ8_9VIRU RNA-directed RNA polymerase OS=Sanxia sobemo-like virus 2 OX=1923381 PE=4 SV=1
MM1 pKa = 7.79DD2 pKa = 4.56VDD4 pKa = 4.86LSSIPGWCVLKK15 pKa = 11.01DD16 pKa = 3.37LGTTNRR22 pKa = 11.84EE23 pKa = 3.78VFKK26 pKa = 10.98VDD28 pKa = 2.99EE29 pKa = 4.42EE30 pKa = 4.36GRR32 pKa = 11.84YY33 pKa = 9.75NLDD36 pKa = 3.09RR37 pKa = 11.84VKK39 pKa = 10.34MVIEE43 pKa = 4.03IVKK46 pKa = 9.58KK47 pKa = 8.66RR48 pKa = 11.84WMEE51 pKa = 3.86IKK53 pKa = 10.46EE54 pKa = 4.16SDD56 pKa = 3.98PIYY59 pKa = 10.88AFVKK63 pKa = 9.98PEE65 pKa = 3.48PHH67 pKa = 6.79KK68 pKa = 10.74RR69 pKa = 11.84EE70 pKa = 3.96KK71 pKa = 10.97LNSGRR76 pKa = 11.84LRR78 pKa = 11.84LISGISLIDD87 pKa = 3.57SLIDD91 pKa = 3.06RR92 pKa = 11.84LLFMKK97 pKa = 8.21WTLKK101 pKa = 8.3TRR103 pKa = 11.84QMLTKK108 pKa = 10.25TPIAVGWTPLDD119 pKa = 3.26AVKK122 pKa = 9.89FHH124 pKa = 7.39HH125 pKa = 6.52MMGGMHH131 pKa = 6.29EE132 pKa = 4.42QYY134 pKa = 11.44LDD136 pKa = 3.44VDD138 pKa = 3.95KK139 pKa = 11.51SAWDD143 pKa = 3.38WSLKK147 pKa = 10.05PWILDD152 pKa = 3.11MVKK155 pKa = 10.16RR156 pKa = 11.84VLCGLVVAPTWWHH169 pKa = 4.13QRR171 pKa = 11.84VNKK174 pKa = 10.16RR175 pKa = 11.84FEE177 pKa = 4.34LLFGNPIWKK186 pKa = 9.22FQDD189 pKa = 2.88SSMIQQKK196 pKa = 9.79QPGIMKK202 pKa = 9.38SGCYY206 pKa = 6.02MTIWINSIGQLILHH220 pKa = 6.16QLAQSRR226 pKa = 11.84CGVQMHH232 pKa = 5.84VPMVVGDD239 pKa = 4.45DD240 pKa = 3.63TTQPYY245 pKa = 10.36YY246 pKa = 11.23GEE248 pKa = 5.46DD249 pKa = 2.95VDD251 pKa = 6.37AYY253 pKa = 10.88LQEE256 pKa = 3.94TRR258 pKa = 11.84NLGFVIKK265 pKa = 10.02PSIGRR270 pKa = 11.84IAEE273 pKa = 3.99FCGFRR278 pKa = 11.84FDD280 pKa = 4.72GFKK283 pKa = 10.8SLPAYY288 pKa = 9.93RR289 pKa = 11.84SKK291 pKa = 10.92HH292 pKa = 4.19QFLLRR297 pKa = 11.84HH298 pKa = 5.76LTLDD302 pKa = 4.92DD303 pKa = 5.12EE304 pKa = 5.03IATQTIQSYY313 pKa = 10.02QLLYY317 pKa = 11.35YY318 pKa = 9.37NDD320 pKa = 4.36KK321 pKa = 11.04YY322 pKa = 11.45ALQQIRR328 pKa = 11.84TLAIQRR334 pKa = 11.84GLLKK338 pKa = 10.63AVVPDD343 pKa = 3.56HH344 pKa = 6.11VLWLIIDD351 pKa = 4.61GLQTT355 pKa = 3.32

Molecular weight: 41.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1032

355

677

516.0

59.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.233 ± 0.868

0.969 ± 0.243

6.977 ± 0.119

6.492 ± 1.408

3.391 ± 0.149

5.717 ± 0.265

2.229 ± 0.325

5.814 ± 0.99

8.14 ± 0.606

9.593 ± 0.925

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.973 ± 0.016

3.585 ± 0.736

3.488 ± 0.407

4.748 ± 0.645

5.62 ± 0.008

7.752 ± 1.482

4.748 ± 0.022

6.686 ± 0.197

1.647 ± 0.957

3.198 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski