Bacillus sp. Y1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4676 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385NL31|A0A385NL31_9BACI Amidohydrolase OS=Bacillus sp. Y1 OX=352858 GN=DOE78_04945 PE=4 SV=1
MM1 pKa = 7.55SYY3 pKa = 10.52KK4 pKa = 10.1IPALILSTTLFAASLFLTNDD24 pKa = 3.08EE25 pKa = 4.38QAQSTTNKK33 pKa = 9.49EE34 pKa = 3.63ATTAAVQTTSSTSFTEE50 pKa = 4.26EE51 pKa = 3.6TASAGSIVEE60 pKa = 3.85EE61 pKa = 4.07DD62 pKa = 3.66VVEE65 pKa = 4.17EE66 pKa = 3.99EE67 pKa = 4.7QEE69 pKa = 3.8ITEE72 pKa = 4.27ILLDD76 pKa = 3.94VPLLNQMDD84 pKa = 4.19SPRR87 pKa = 11.84LYY89 pKa = 10.48NGCEE93 pKa = 3.99VTSLAMILQYY103 pKa = 11.18NGLDD107 pKa = 3.37VTKK110 pKa = 10.41NQLAEE115 pKa = 4.24EE116 pKa = 4.6VVRR119 pKa = 11.84TPLQYY124 pKa = 11.22NSGEE128 pKa = 4.11YY129 pKa = 10.7GNPNIGFVGNMEE141 pKa = 5.24DD142 pKa = 4.15GPGLGVYY149 pKa = 9.95HH150 pKa = 6.71GPIYY154 pKa = 10.81DD155 pKa = 3.87LATNYY160 pKa = 10.46SDD162 pKa = 5.97DD163 pKa = 5.21VIDD166 pKa = 4.53LTNQPFDD173 pKa = 4.58DD174 pKa = 4.46LLKK177 pKa = 10.6QVAQGHH183 pKa = 5.57PVWIITTSTFSTIDD197 pKa = 3.38EE198 pKa = 4.38FEE200 pKa = 4.05KK201 pKa = 10.57WEE203 pKa = 4.27TPQGEE208 pKa = 4.21VTITYY213 pKa = 10.11KK214 pKa = 9.26MHH216 pKa = 6.08SVVITGYY223 pKa = 10.33DD224 pKa = 3.31NEE226 pKa = 4.87SIYY229 pKa = 10.87INNPYY234 pKa = 10.46GEE236 pKa = 4.85RR237 pKa = 11.84NQQLDD242 pKa = 3.76RR243 pKa = 11.84EE244 pKa = 4.56SFIQAWEE251 pKa = 4.03QMGSQAIVVLL261 pKa = 4.23

Molecular weight: 28.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385NUT4|A0A385NUT4_9BACI Purine nucleoside phosphorylase DeoD-type OS=Bacillus sp. Y1 OX=352858 GN=deoD PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4676

0

4676

1408612

25

2428

301.2

33.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.674 ± 0.033

0.714 ± 0.011

4.95 ± 0.031

7.602 ± 0.04

4.614 ± 0.03

6.908 ± 0.033

2.077 ± 0.018

7.886 ± 0.039

6.814 ± 0.031

9.869 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.018

4.444 ± 0.028

3.554 ± 0.021

3.652 ± 0.022

3.87 ± 0.024

6.276 ± 0.031

5.538 ± 0.029

7.2 ± 0.026

1.052 ± 0.015

3.578 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski