Streptococcus satellite phage Javan746
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZVD1|A0A4D5ZVD1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan746 OX=2558840 GN=JavanS746_0006 PE=4 SV=1
MM1 pKa = 7.76 SYY3 pKa = 10.21 TMNLYY8 pKa = 10.02 PDD10 pKa = 3.46 IEE12 pKa = 4.83 KK13 pKa = 10.53 IPDD16 pKa = 3.08 EE17 pKa = 4.28 NYY19 pKa = 6.67 FTKK22 pKa = 10.56 EE23 pKa = 3.53 IFLAMCTDD31 pKa = 3.57 NATKK35 pKa = 10.5 LAVTMGLLAKK45 pKa = 10.11 QALDD49 pKa = 3.49 LEE51 pKa = 4.63 TRR53 pKa = 11.84 AVILKK58 pKa = 8.09 TLNTIIEE65 pKa = 4.15 NQKK68 pKa = 11.03 AILQGVASGQITFLNKK84 pKa = 10.26 SKK86 pKa = 11.23 GEE88 pKa = 4.06 EE89 pKa = 4.08 EE90 pKa = 3.99 LDD92 pKa = 3.42 EE93 pKa = 5.08 SIRR96 pKa = 11.84 EE97 pKa = 4.11
Molecular weight: 10.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.746
IPC2_protein 4.736
IPC_protein 4.558
Toseland 4.418
ProMoST 4.558
Dawson 4.495
Bjellqvist 4.711
Wikipedia 4.342
Rodwell 4.393
Grimsley 4.329
Solomon 4.482
Lehninger 4.431
Nozaki 4.596
DTASelect 4.698
Thurlkill 4.406
EMBOSS 4.355
Sillero 4.66
Patrickios 4.088
IPC_peptide 4.495
IPC2_peptide 4.66
IPC2.peptide.svr19 4.601
Protein with the highest isoelectric point:
>tr|A0A4D6A054|A0A4D6A054_9VIRU Clp protease-like protein OS=Streptococcus satellite phage Javan746 OX=2558840 GN=JavanS746_0015 PE=3 SV=1
MM1 pKa = 7.11 EE2 pKa = 5.89 AKK4 pKa = 8.94 THH6 pKa = 6.09 FARR9 pKa = 11.84 FMRR12 pKa = 11.84 RR13 pKa = 11.84 GMEE16 pKa = 4.44 LARR19 pKa = 11.84 QLHH22 pKa = 5.78 SKK24 pKa = 9.95 EE25 pKa = 3.78 IQRR28 pKa = 11.84 DD29 pKa = 3.48 EE30 pKa = 4.84 FDD32 pKa = 3.9 RR33 pKa = 11.84 AWKK36 pKa = 10.38 RR37 pKa = 11.84 LGDD40 pKa = 3.58
Molecular weight: 4.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.15
IPC2_protein 9.194
IPC_protein 9.516
Toseland 10.599
ProMoST 10.087
Dawson 10.628
Bjellqvist 10.233
Wikipedia 10.76
Rodwell 10.891
Grimsley 10.643
Solomon 10.76
Lehninger 10.76
Nozaki 10.555
DTASelect 10.233
Thurlkill 10.555
EMBOSS 10.979
Sillero 10.555
Patrickios 10.774
IPC_peptide 10.774
IPC2_peptide 8.595
IPC2.peptide.svr19 8.588
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2497
40
484
166.5
19.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.086 ± 0.518
0.561 ± 0.089
5.527 ± 0.661
8.971 ± 0.907
4.245 ± 0.622
5.326 ± 0.423
1.762 ± 0.268
6.247 ± 0.465
10.533 ± 0.521
10.412 ± 1.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.403 ± 0.427
5.086 ± 0.225
2.283 ± 0.217
4.285 ± 0.639
5.166 ± 0.682
5.366 ± 0.437
5.406 ± 0.596
5.687 ± 0.483
0.881 ± 0.131
4.766 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here