Panteoa phage Kyle
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A514A8I2|A0A514A8I2_9CAUD Uncharacterized protein OS=Panteoa phage Kyle OX=2589665 GN=42 PE=4 SV=1
MM1 pKa = 7.67 AKK3 pKa = 9.02 LTATEE8 pKa = 4.34 LANASAFFAGEE19 pKa = 3.98 YY20 pKa = 10.14 VPYY23 pKa = 10.85 ASLLAANVGVKK34 pKa = 9.67 IGEE37 pKa = 4.34 EE38 pKa = 3.73 IPAEE42 pKa = 4.15 VVGDD46 pKa = 3.82 FGFDD50 pKa = 3.78 DD51 pKa = 4.2 EE52 pKa = 4.77 EE53 pKa = 4.67 GAYY56 pKa = 10.64 LDD58 pKa = 4.06 TVDD61 pKa = 3.46 GRR63 pKa = 11.84 EE64 pKa = 3.63 IRR66 pKa = 11.84 VIEE69 pKa = 4.16 TEE71 pKa = 4.43 LYY73 pKa = 9.91 VAAEE77 pKa = 4.17 DD78 pKa = 3.99 VATGEE83 pKa = 4.27 VAFFALEE90 pKa = 4.29 EE91 pKa = 4.1 IEE93 pKa = 4.28 EE94 pKa = 4.21
Molecular weight: 10.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.105
IPC2_protein 3.706
IPC_protein 3.592
Toseland 3.427
ProMoST 3.706
Dawson 3.554
Bjellqvist 3.783
Wikipedia 3.439
Rodwell 3.439
Grimsley 3.35
Solomon 3.528
Lehninger 3.478
Nozaki 3.694
DTASelect 3.77
Thurlkill 3.478
EMBOSS 3.465
Sillero 3.706
Patrickios 2.854
IPC_peptide 3.528
IPC2_peptide 3.681
IPC2.peptide.svr19 3.698
Protein with the highest isoelectric point:
>tr|A0A514A8P6|A0A514A8P6_9CAUD Uncharacterized protein OS=Panteoa phage Kyle OX=2589665 GN=20 PE=4 SV=1
MM1 pKa = 6.92 AAKK4 pKa = 10.09 RR5 pKa = 11.84 RR6 pKa = 11.84 FRR8 pKa = 11.84 LMRR11 pKa = 11.84 IGNILPGTRR20 pKa = 11.84 FWFVYY25 pKa = 9.33 GKK27 pKa = 10.05 KK28 pKa = 9.96 RR29 pKa = 11.84 PGTLIWLNKK38 pKa = 9.7 KK39 pKa = 9.69 NVACYY44 pKa = 10.23 HH45 pKa = 6.59 IGHH48 pKa = 6.41 SFNVKK53 pKa = 9.53 ARR55 pKa = 11.84 RR56 pKa = 11.84 FTIGAWCQVWVEE68 pKa = 3.83 IPDD71 pKa = 4.0 YY72 pKa = 10.53 EE73 pKa = 4.27 KK74 pKa = 10.88 HH75 pKa = 5.77
Molecular weight: 8.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.999
IPC_protein 10.818
Toseland 10.979
ProMoST 10.716
Dawson 11.052
Bjellqvist 10.774
Wikipedia 11.286
Rodwell 11.345
Grimsley 11.096
Solomon 11.213
Lehninger 11.169
Nozaki 10.965
DTASelect 10.774
Thurlkill 10.979
EMBOSS 11.389
Sillero 10.994
Patrickios 11.096
IPC_peptide 11.213
IPC2_peptide 9.867
IPC2.peptide.svr19 8.272
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
106
0
106
21707
37
1003
204.8
22.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.942 ± 0.283
0.912 ± 0.077
5.855 ± 0.151
6.8 ± 0.324
4.229 ± 0.138
7.159 ± 0.233
1.829 ± 0.141
5.901 ± 0.18
6.408 ± 0.28
7.638 ± 0.232
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.847 ± 0.102
4.915 ± 0.223
3.884 ± 0.142
3.962 ± 0.174
5.215 ± 0.203
6.159 ± 0.219
5.823 ± 0.213
7.039 ± 0.249
1.354 ± 0.097
3.128 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here