Peptoniphilus sp. oral taxon 386 str. F0131

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Peptoniphilus; unclassified Peptoniphilus; Peptoniphilus sp. oral taxon 386

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7N654|D7N654_9FIRM Putative general secretory pathway protein E OS=Peptoniphilus sp. oral taxon 386 str. F0131 OX=575609 GN=HMPREF0629_01014 PE=3 SV=1
MM1 pKa = 7.7EE2 pKa = 5.78GNTMTEE8 pKa = 4.08EE9 pKa = 4.19KK10 pKa = 10.78KK11 pKa = 10.7NGCCCCGHH19 pKa = 5.94DD20 pKa = 5.08HH21 pKa = 5.53EE22 pKa = 6.31HH23 pKa = 5.22EE24 pKa = 4.67HH25 pKa = 5.85EE26 pKa = 4.52AEE28 pKa = 4.01EE29 pKa = 4.68FEE31 pKa = 4.9TIVLTLDD38 pKa = 4.0DD39 pKa = 5.46DD40 pKa = 4.81SEE42 pKa = 4.75LEE44 pKa = 4.18CVILGIFDD52 pKa = 3.67YY53 pKa = 10.86EE54 pKa = 4.07EE55 pKa = 3.92RR56 pKa = 11.84SYY58 pKa = 11.28IALLPANEE66 pKa = 4.17EE67 pKa = 4.09DD68 pKa = 3.96GEE70 pKa = 4.44EE71 pKa = 3.71ILIYY75 pKa = 10.39DD76 pKa = 4.3FKK78 pKa = 11.04EE79 pKa = 4.07LEE81 pKa = 4.13NEE83 pKa = 4.02EE84 pKa = 4.43VEE86 pKa = 4.42LNVIEE91 pKa = 5.95DD92 pKa = 3.68EE93 pKa = 4.27EE94 pKa = 4.63LFNKK98 pKa = 9.32VSKK101 pKa = 10.43EE102 pKa = 3.75FEE104 pKa = 3.74ALYY107 pKa = 10.82VEE109 pKa = 5.02EE110 pKa = 4.42EE111 pKa = 4.1

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7N8C3|D7N8C3_9FIRM R3H domain protein OS=Peptoniphilus sp. oral taxon 386 str. F0131 OX=575609 GN=HMPREF0629_00316 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.18NKK10 pKa = 8.76QRR12 pKa = 11.84KK13 pKa = 7.85RR14 pKa = 11.84EE15 pKa = 3.9HH16 pKa = 6.14GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84NVIKK32 pKa = 10.48ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.14GRR39 pKa = 11.84KK40 pKa = 8.67ILSAA44 pKa = 4.02

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1404

0

1404

447445

37

2878

318.7

36.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.675 ± 0.057

0.964 ± 0.021

5.795 ± 0.052

7.841 ± 0.078

4.591 ± 0.053

6.296 ± 0.056

1.261 ± 0.021

9.856 ± 0.081

9.008 ± 0.066

9.034 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.032

6.243 ± 0.056

2.711 ± 0.037

2.141 ± 0.032

3.628 ± 0.045

6.39 ± 0.052

4.757 ± 0.053

6.411 ± 0.054

0.573 ± 0.021

4.216 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski