Termitomyces sp. J132

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Lyophyllaceae; Termitomyces; unclassified Termitomyces

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L6W9X5|A0A0L6W9X5_9AGAR Pkinase_fungal domain-containing protein OS=Termitomyces sp. J132 OX=1306850 GN=J132_04020 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.4LIPLLTSLISATVTIVYY19 pKa = 7.67ATPVHH24 pKa = 6.25SGSLVTTDD32 pKa = 3.56GDD34 pKa = 4.31SVTTTGISSSTTVTLSEE51 pKa = 4.21TAIVTPTLTACSLDD65 pKa = 3.71TTDD68 pKa = 5.67DD69 pKa = 3.86CLLPTVAVSTILPVFSSVVTSEE91 pKa = 4.35PGATTANPILATVTALPSEE110 pKa = 4.38PLEE113 pKa = 4.56APISPCPLPVTVIFTVVNGLLGTSALPSTPTTTTSGVDD151 pKa = 3.53TIVPPILASDD161 pKa = 4.13SSALPTAVPSDD172 pKa = 3.97TILPSILVSDD182 pKa = 4.41LSEE185 pKa = 4.53FPTAVPSDD193 pKa = 3.28TSVRR197 pKa = 11.84LFHH200 pKa = 7.07PRR202 pKa = 11.84LL203 pKa = 3.55

Molecular weight: 20.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L6WLU5|A0A0L6WLU5_9AGAR Uncharacterized protein OS=Termitomyces sp. J132 OX=1306850 GN=J132_10223 PE=4 SV=1
MM1 pKa = 7.72LCTQKK6 pKa = 10.61CHH8 pKa = 7.38DD9 pKa = 4.88PFPEE13 pKa = 4.1TLHH16 pKa = 6.79IVHH19 pKa = 5.79QTPEE23 pKa = 4.24PPKK26 pKa = 10.73GPPTPRR32 pKa = 11.84RR33 pKa = 11.84TSAHH37 pKa = 6.18APRR40 pKa = 11.84LLRR43 pKa = 11.84LTPTPPRR50 pKa = 11.84LTPTPPRR57 pKa = 11.84LTPTPPRR64 pKa = 11.84LTPTPPRR71 pKa = 11.84LTPTPPRR78 pKa = 11.84LTPTPPRR85 pKa = 11.84LTPTPPRR92 pKa = 11.84LTPTPPRR99 pKa = 11.84LTPTPPRR106 pKa = 11.84LTPTPPRR113 pKa = 11.84LTPTPPRR120 pKa = 11.84LTPTPPRR127 pKa = 11.84LTPTPPRR134 pKa = 11.84LTPTPPRR141 pKa = 11.84LTPTPPRR148 pKa = 11.84LTPTPPRR155 pKa = 11.84LTPTPPRR162 pKa = 11.84LTPTPPRR169 pKa = 11.84LTPTPPRR176 pKa = 11.84LTPTPPRR183 pKa = 11.84LTPTPPRR190 pKa = 11.84LTPTPPRR197 pKa = 11.84LTPTPPRR204 pKa = 11.84LTPTPPRR211 pKa = 11.84LTPTPPRR218 pKa = 11.84LTPTPPRR225 pKa = 11.84LTPTPPRR232 pKa = 11.84LTPTPPRR239 pKa = 11.84LTPTPPRR246 pKa = 11.84LTPTPPRR253 pKa = 11.84LTPTPPRR260 pKa = 11.84LTPTAPWPTLVPAPQPLTLTLGFPNPKK287 pKa = 9.26NHH289 pKa = 6.54PRR291 pKa = 11.84YY292 pKa = 10.09SS293 pKa = 3.29

Molecular weight: 32.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11124

0

11124

4621127

11

5053

415.4

46.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.96 ± 0.018

1.266 ± 0.009

5.539 ± 0.017

5.953 ± 0.024

3.833 ± 0.015

6.214 ± 0.02

2.624 ± 0.012

5.284 ± 0.017

4.783 ± 0.021

9.486 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.061 ± 0.01

3.746 ± 0.012

6.298 ± 0.032

3.825 ± 0.016

5.856 ± 0.02

8.564 ± 0.033

6.133 ± 0.017

6.402 ± 0.015

1.42 ± 0.009

2.712 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski