Jurona vesiculovirus
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I1SV84|I1SV84_9RHAB Glycoprotein OS=Jurona vesiculovirus OX=1972568 PE=3 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.65 KK3 pKa = 9.09 MLTGINMSALKK14 pKa = 10.33 NVLKK18 pKa = 10.53 SYY20 pKa = 10.83 PNLKK24 pKa = 8.46 QTFDD28 pKa = 3.8 EE29 pKa = 4.4 LNEE32 pKa = 3.99 MEE34 pKa = 4.66 EE35 pKa = 4.29 NGSVLQNPTNEE46 pKa = 4.33 DD47 pKa = 3.18 VSVEE51 pKa = 4.15 HH52 pKa = 7.03 PPLYY56 pKa = 10.38 HH57 pKa = 6.96 SLDD60 pKa = 3.44 ILSEE64 pKa = 4.07 SSEE67 pKa = 3.87 EE68 pKa = 4.01 DD69 pKa = 3.33 TEE71 pKa = 4.35 EE72 pKa = 3.99 EE73 pKa = 5.18 SEE75 pKa = 4.49 VEE77 pKa = 4.77 DD78 pKa = 3.94 LGEE81 pKa = 4.06 MEE83 pKa = 4.74 NVSVDD88 pKa = 3.71 DD89 pKa = 4.93 EE90 pKa = 4.62 KK91 pKa = 11.58 DD92 pKa = 3.25 QSEE95 pKa = 5.05 EE96 pKa = 3.82 IDD98 pKa = 3.71 DD99 pKa = 5.17 DD100 pKa = 4.02 NFHH103 pKa = 7.21 VSFNEE108 pKa = 3.83 KK109 pKa = 10.61 LPWTAMTQKK118 pKa = 9.19 TVNGQLRR125 pKa = 11.84 VNMSAPEE132 pKa = 4.05 GLNEE136 pKa = 4.04 LQFEE140 pKa = 4.38 QWVSSIEE147 pKa = 3.94 NLMSLSKK154 pKa = 10.48 SLRR157 pKa = 11.84 LHH159 pKa = 5.69 SAEE162 pKa = 4.7 LSIIEE167 pKa = 5.09 DD168 pKa = 4.88 GIQIDD173 pKa = 4.15 EE174 pKa = 4.73 KK175 pKa = 11.4 LNSCLSRR182 pKa = 11.84 TSCFKK187 pKa = 10.85 ALPEE191 pKa = 4.36 FKK193 pKa = 10.69 DD194 pKa = 3.3 PAGEE198 pKa = 4.2 KK199 pKa = 10.08 KK200 pKa = 10.65 GSEE203 pKa = 4.23 NLTIASSPSGSCEE216 pKa = 4.04 SEE218 pKa = 4.08 SSDD221 pKa = 4.76 LSGLPSLHH229 pKa = 6.24 QEE231 pKa = 4.2 EE232 pKa = 4.62 IKK234 pKa = 11.35 NMIEE238 pKa = 3.73 EE239 pKa = 4.2 KK240 pKa = 10.54 FILRR244 pKa = 11.84 SEE246 pKa = 4.13 DD247 pKa = 3.63 KK248 pKa = 10.58 KK249 pKa = 10.81 CKK251 pKa = 9.77 EE252 pKa = 3.89 YY253 pKa = 8.89 HH254 pKa = 5.6 TSFAEE259 pKa = 4.06 LFGSKK264 pKa = 9.04 EE265 pKa = 3.53 AALAFLNGNPVGIDD279 pKa = 3.39 EE280 pKa = 4.78 LVCAGLKK287 pKa = 10.05 RR288 pKa = 11.84 RR289 pKa = 11.84 GIFNRR294 pKa = 11.84 MRR296 pKa = 11.84 IKK298 pKa = 10.83 YY299 pKa = 8.23 VLKK302 pKa = 10.48 PIFKK306 pKa = 10.48
Molecular weight: 34.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.692
IPC2_protein 4.622
IPC_protein 4.546
Toseland 4.406
ProMoST 4.622
Dawson 4.469
Bjellqvist 4.622
Wikipedia 4.304
Rodwell 4.38
Grimsley 4.317
Solomon 4.469
Lehninger 4.418
Nozaki 4.571
DTASelect 4.673
Thurlkill 4.393
EMBOSS 4.329
Sillero 4.647
Patrickios 4.037
IPC_peptide 4.482
IPC2_peptide 4.647
IPC2.peptide.svr19 4.575
Protein with the highest isoelectric point:
>tr|I1SV83|I1SV83_9RHAB Phosphoprotein OS=Jurona vesiculovirus OX=1972568 PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.25 SFKK5 pKa = 11.01 GMFKK9 pKa = 10.56 NYY11 pKa = 9.8 KK12 pKa = 9.38 EE13 pKa = 3.99 KK14 pKa = 11.36 NKK16 pKa = 9.99 VKK18 pKa = 10.81 KK19 pKa = 10.5 EE20 pKa = 4.01 MDD22 pKa = 3.04 WDD24 pKa = 4.13 SPPSYY29 pKa = 9.88 TDD31 pKa = 2.95 VRR33 pKa = 11.84 RR34 pKa = 11.84 GIYY37 pKa = 9.85 PSAPLFGLDD46 pKa = 3.61 DD47 pKa = 4.3 SFMEE51 pKa = 4.45 TLPSLGIQNMKK62 pKa = 10.16 LQYY65 pKa = 9.88 KK66 pKa = 10.35 CSIQLRR72 pKa = 11.84 AEE74 pKa = 4.34 FPFVSYY80 pKa = 11.1 SEE82 pKa = 4.4 VVMALSQWDD91 pKa = 3.5 EE92 pKa = 4.15 EE93 pKa = 4.5 YY94 pKa = 10.87 RR95 pKa = 11.84 GFLGKK100 pKa = 10.27 RR101 pKa = 11.84 PFYY104 pKa = 10.37 RR105 pKa = 11.84 AIILRR110 pKa = 11.84 TSKK113 pKa = 9.25 TLKK116 pKa = 10.36 AVPLSLTDD124 pKa = 3.49 GGRR127 pKa = 11.84 PEE129 pKa = 4.34 YY130 pKa = 10.6 NGEE133 pKa = 3.92 IQGQSSLYY141 pKa = 9.34 HH142 pKa = 5.95 SLGIIPPMMYY152 pKa = 10.19 VPEE155 pKa = 4.22 TFMKK159 pKa = 9.72 EE160 pKa = 3.21 WKK162 pKa = 8.28 TAGNRR167 pKa = 11.84 GILIIKK173 pKa = 8.98 IWLGITDD180 pKa = 4.33 TLDD183 pKa = 3.33 TLDD186 pKa = 4.68 PLLHH190 pKa = 6.11 PMIFKK195 pKa = 10.26 SEE197 pKa = 4.11 RR198 pKa = 11.84 EE199 pKa = 4.18 LVEE202 pKa = 3.63 MAKK205 pKa = 10.21 VFNLDD210 pKa = 2.92 ISKK213 pKa = 10.55 GRR215 pKa = 11.84 DD216 pKa = 3.33 NNWIISRR223 pKa = 11.84 SYY225 pKa = 10.86
Molecular weight: 26.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.552
IPC2_protein 8.507
IPC_protein 8.463
Toseland 9.077
ProMoST 8.99
Dawson 9.385
Bjellqvist 9.107
Wikipedia 9.56
Rodwell 9.589
Grimsley 9.458
Solomon 9.458
Lehninger 9.414
Nozaki 9.121
DTASelect 9.092
Thurlkill 9.209
EMBOSS 9.516
Sillero 9.326
Patrickios 4.66
IPC_peptide 9.443
IPC2_peptide 7.644
IPC2.peptide.svr19 7.661
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3567
225
2090
713.4
80.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.486 ± 0.67
1.71 ± 0.398
5.719 ± 0.322
6.224 ± 1.135
4.093 ± 0.239
6.28 ± 0.431
2.467 ± 0.363
6.869 ± 0.495
6.897 ± 0.33
10.177 ± 0.769
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.747 ± 0.313
4.878 ± 0.392
4.429 ± 0.556
3.196 ± 0.152
4.99 ± 0.637
9.532 ± 0.697
5.018 ± 0.438
5.13 ± 0.462
1.99 ± 0.239
3.168 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here