Microbacterium phage Schubert

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Schubertvirus; Microbacterium virus Schubert

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9RAA5|A0A3Q9RAA5_9CAUD Uncharacterized protein OS=Microbacterium phage Schubert OX=2500787 GN=49 PE=4 SV=1
MM1 pKa = 7.15NNEE4 pKa = 3.84ARR6 pKa = 11.84ILDD9 pKa = 3.56HH10 pKa = 5.92TTTVFSTDD18 pKa = 3.45LVLDD22 pKa = 3.98HH23 pKa = 6.82YY24 pKa = 11.39GYY26 pKa = 10.65LAPRR30 pKa = 11.84TVTVEE35 pKa = 4.09GIEE38 pKa = 4.64FVLGDD43 pKa = 3.61NGYY46 pKa = 10.6EE47 pKa = 3.96NLEE50 pKa = 4.4LGVTLYY56 pKa = 11.23LEE58 pKa = 4.31STEE61 pKa = 4.4DD62 pKa = 3.54ARR64 pKa = 11.84TADD67 pKa = 4.3DD68 pKa = 3.06VWYY71 pKa = 9.99AQIDD75 pKa = 4.32PISS78 pKa = 3.47

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0INY4|A0A3T0INY4_9CAUD Uncharacterized protein OS=Microbacterium phage Schubert OX=2500787 GN=1 PE=4 SV=1
MM1 pKa = 7.52AMQRR5 pKa = 11.84RR6 pKa = 11.84VVVRR10 pKa = 11.84NPSAFKK16 pKa = 9.14QTLTIKK22 pKa = 10.78VKK24 pKa = 9.67VTTTDD29 pKa = 3.29GNVKK33 pKa = 8.17ITDD36 pKa = 4.14MILQAGTTGTGWVPNVTEE54 pKa = 4.51MPWTTGVVSS63 pKa = 3.9

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12285

48

808

223.4

24.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.646 ± 0.355

0.611 ± 0.097

6.195 ± 0.299

6.414 ± 0.416

2.882 ± 0.174

7.969 ± 0.392

1.88 ± 0.174

5.25 ± 0.433

4.64 ± 0.328

8.433 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.128

3.484 ± 0.191

4.713 ± 0.237

4.176 ± 0.194

5.885 ± 0.359

6.064 ± 0.28

6.903 ± 0.375

7.253 ± 0.313

2.019 ± 0.202

3.15 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski