Wenling frogfish arenavirus 2
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GNQ8|A0A2P1GNQ8_9VIRU Replicase OS=Wenling frogfish arenavirus 2 OX=2116467 PE=4 SV=1
MM1 pKa = 7.79 AGTSDD6 pKa = 3.77 EE7 pKa = 4.21 NQLTFEE13 pKa = 4.55 EE14 pKa = 4.8 LKK16 pKa = 10.47 QLHH19 pKa = 6.16 GANHH23 pKa = 6.62 VIDD26 pKa = 5.54 DD27 pKa = 3.94 NALVEE32 pKa = 4.24 VKK34 pKa = 10.36 IIFDD38 pKa = 4.08 KK39 pKa = 11.31 LDD41 pKa = 3.29 GAIIKK46 pKa = 10.14 EE47 pKa = 4.14 LDD49 pKa = 3.66 DD50 pKa = 3.44 MSKK53 pKa = 10.71 RR54 pKa = 11.84 IAKK57 pKa = 10.03 SEE59 pKa = 4.03 LKK61 pKa = 10.59 DD62 pKa = 4.0 YY63 pKa = 10.99 IDD65 pKa = 5.41 DD66 pKa = 4.33 LSDD69 pKa = 3.3 VNRR72 pKa = 11.84 KK73 pKa = 8.63 IRR75 pKa = 11.84 SLHH78 pKa = 4.59 TSEE81 pKa = 4.48 YY82 pKa = 9.91 VSEE85 pKa = 5.05 GSQEE89 pKa = 3.96 PVQLSINEE97 pKa = 3.99 LTAGEE102 pKa = 4.56 VVQLDD107 pKa = 3.57 QDD109 pKa = 3.7 IEE111 pKa = 4.29 DD112 pKa = 3.87 MRR114 pKa = 11.84 KK115 pKa = 9.82 KK116 pKa = 10.83 SNTGRR121 pKa = 11.84 GTGNKK126 pKa = 9.68 DD127 pKa = 2.63 NWISGCEE134 pKa = 3.87 KK135 pKa = 10.67 DD136 pKa = 5.76 LEE138 pKa = 4.44 LLKK141 pKa = 10.46 STGYY145 pKa = 11.17 FDD147 pKa = 3.49 MSKK150 pKa = 11.08 GNPQSLANYY159 pKa = 8.59 CLEE162 pKa = 4.32 AGRR165 pKa = 11.84 EE166 pKa = 4.12 LEE168 pKa = 4.47 LNQALEE174 pKa = 4.48 DD175 pKa = 3.95 SLILTDD181 pKa = 3.39 EE182 pKa = 4.45 DD183 pKa = 3.33 KK184 pKa = 11.58 ARR186 pKa = 11.84 FVTEE190 pKa = 3.68 KK191 pKa = 10.97 RR192 pKa = 11.84 EE193 pKa = 3.86 GLAAANHH200 pKa = 5.75 FQVDD204 pKa = 4.09 PLFLLALFMKK214 pKa = 10.46 ASQLTWQDD222 pKa = 3.25 CVKK225 pKa = 10.63 CITILKK231 pKa = 7.51 TLLMEE236 pKa = 5.06 RR237 pKa = 11.84 PLSLKK242 pKa = 10.84 LKK244 pKa = 9.95 DD245 pKa = 3.81 QLGKK249 pKa = 10.69 KK250 pKa = 9.27 PGGVFSFLNTLYY262 pKa = 10.39 EE263 pKa = 4.44 GKK265 pKa = 10.38 LGLFGSPFIMTRR277 pKa = 11.84 ARR279 pKa = 11.84 LTYY282 pKa = 9.42 CLSVMYY288 pKa = 8.76 GIPSRR293 pKa = 11.84 LAISASRR300 pKa = 11.84 GVPSLIKK307 pKa = 10.77 DD308 pKa = 3.36 MLKK311 pKa = 9.91 IRR313 pKa = 11.84 ITKK316 pKa = 10.06 KK317 pKa = 10.76 LIISPDD323 pKa = 3.63 RR324 pKa = 11.84 EE325 pKa = 3.8 DD326 pKa = 3.66 STWYY330 pKa = 9.79 EE331 pKa = 4.27 HH332 pKa = 6.07 GCHH335 pKa = 6.04 RR336 pKa = 11.84 VFTTPDD342 pKa = 3.55 PEE344 pKa = 4.85 DD345 pKa = 3.13 VWKK348 pKa = 10.94 NRR350 pKa = 11.84 TAVVKK355 pKa = 10.69 NGLSTVVINTKK366 pKa = 10.58 ADD368 pKa = 3.36 LRR370 pKa = 11.84 SVKK373 pKa = 9.74 EE374 pKa = 3.57 VAAAPKK380 pKa = 10.06 SSVRR384 pKa = 11.84 RR385 pKa = 11.84 SPEE388 pKa = 3.59 NKK390 pKa = 7.88 QTIAKK395 pKa = 8.12 TSSHH399 pKa = 6.3 LPIDD403 pKa = 3.63 TFLVRR408 pKa = 11.84 KK409 pKa = 7.66 TPEE412 pKa = 4.14 SQSSGPFWVIDD423 pKa = 3.59 VEE425 pKa = 5.05 GEE427 pKa = 3.9 AKK429 pKa = 10.56 SPVEE433 pKa = 3.57 ICIMKK438 pKa = 9.92 FDD440 pKa = 4.5 PEE442 pKa = 4.11 IGGKK446 pKa = 10.42 GGIKK450 pKa = 10.26 DD451 pKa = 3.34 RR452 pKa = 11.84 FFRR455 pKa = 11.84 QIKK458 pKa = 10.35 SNIGDD463 pKa = 3.38 SSFTHH468 pKa = 6.79 GLNKK472 pKa = 10.26 SKK474 pKa = 10.87 SRR476 pKa = 11.84 DD477 pKa = 3.68 DD478 pKa = 4.09 VDD480 pKa = 3.22 WADD483 pKa = 3.99 DD484 pKa = 3.59 MRR486 pKa = 11.84 EE487 pKa = 3.79 FWVKK491 pKa = 8.55 VHH493 pKa = 6.27 GNVYY497 pKa = 10.54 CKK499 pKa = 10.44 GSRR502 pKa = 11.84 DD503 pKa = 3.47 IKK505 pKa = 11.22 DD506 pKa = 3.56 FLDD509 pKa = 3.6 NLGLSGTILDD519 pKa = 4.53 LEE521 pKa = 5.2 GIHH524 pKa = 7.05 KK525 pKa = 10.54 SWDD528 pKa = 4.21 DD529 pKa = 3.53 INKK532 pKa = 8.29 TQYY535 pKa = 11.65 ACILDD540 pKa = 4.06 LEE542 pKa = 4.65 STQVCSRR549 pKa = 11.84 KK550 pKa = 10.07 DD551 pKa = 3.15 IHH553 pKa = 7.57 DD554 pKa = 4.62 KK555 pKa = 11.12 LPQKK559 pKa = 10.91 QNGDD563 pKa = 3.41 INKK566 pKa = 9.31 KK567 pKa = 9.68 HH568 pKa = 6.36 LPHH571 pKa = 6.61 CAEE574 pKa = 4.13 VDD576 pKa = 3.99 CLHH579 pKa = 7.21 LICMAMGKK587 pKa = 9.8 VPDD590 pKa = 4.24 EE591 pKa = 4.67 FIKK594 pKa = 10.18 TT595 pKa = 3.6
Molecular weight: 66.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.862
IPC2_protein 5.792
IPC_protein 5.842
Toseland 6.148
ProMoST 6.186
Dawson 6.097
Bjellqvist 6.122
Wikipedia 6.109
Rodwell 6.084
Grimsley 6.262
Solomon 6.097
Lehninger 6.097
Nozaki 6.364
DTASelect 6.547
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.465
Patrickios 4.507
IPC_peptide 6.122
IPC2_peptide 6.402
IPC2.peptide.svr19 6.47
Protein with the highest isoelectric point:
>tr|A0A2P1GNX5|A0A2P1GNX5_9VIRU Glycoprotein OS=Wenling frogfish arenavirus 2 OX=2116467 PE=4 SV=1
MM1 pKa = 7.44 FEE3 pKa = 5.2 SIIDD7 pKa = 4.85 PILEE11 pKa = 3.88 WLFNLFSFILAVIFVMTLSLLIYY34 pKa = 10.44 RR35 pKa = 11.84 MRR37 pKa = 11.84 NLPFLFMMCGSAEE50 pKa = 4.03 CWVSNIQMNGHH61 pKa = 4.84 TAEE64 pKa = 3.87 MRR66 pKa = 11.84 QYY68 pKa = 10.44 EE69 pKa = 4.39 GQWLCGKK76 pKa = 10.14 IKK78 pKa = 9.88 MIKK81 pKa = 10.22 LDD83 pKa = 3.57 DD84 pKa = 4.23 LYY86 pKa = 11.16 YY87 pKa = 10.15 IRR89 pKa = 11.84 YY90 pKa = 9.18 NMSDD94 pKa = 3.55 SAVHH98 pKa = 6.47 LVMSRR103 pKa = 11.84 CTNTKK108 pKa = 10.34 LEE110 pKa = 4.43 NGWEE114 pKa = 4.66 SVCSEE119 pKa = 4.34 RR120 pKa = 11.84 PALLKK125 pKa = 10.85 NISTGISMIAGFNCSGQQKK144 pKa = 10.06 CFVGDD149 pKa = 3.89 GCTPWVDD156 pKa = 3.45 ARR158 pKa = 11.84 DD159 pKa = 3.3 ICGRR163 pKa = 11.84 KK164 pKa = 9.56 LEE166 pKa = 4.47 RR167 pKa = 11.84 KK168 pKa = 9.49 LDD170 pKa = 3.59 NHH172 pKa = 6.56 QMLDD176 pKa = 2.92 ICRR179 pKa = 11.84 QPIVGWIDD187 pKa = 3.17 SDD189 pKa = 4.2 GVHH192 pKa = 6.61 GRR194 pKa = 11.84 MQPIDD199 pKa = 3.35 KK200 pKa = 9.05 STSVKK205 pKa = 10.54 EE206 pKa = 3.87 IDD208 pKa = 3.65 STSSGGHH215 pKa = 4.65 WNIAGRR221 pKa = 11.84 CSCIAYY227 pKa = 8.91 HH228 pKa = 6.23 QGVALPPITLNRR240 pKa = 11.84 HH241 pKa = 5.13 NSFCAADD248 pKa = 3.62 EE249 pKa = 4.34 WFSTHH254 pKa = 6.56 PKK256 pKa = 10.39 VGTKK260 pKa = 9.93 VCISADD266 pKa = 3.36 SLAVNFLKK274 pKa = 10.48 WSMKK278 pKa = 9.56 VMNHH282 pKa = 5.67 KK283 pKa = 10.28 QDD285 pKa = 3.43 IIAVEE290 pKa = 3.97 KK291 pKa = 10.66 GGTVNYY297 pKa = 10.09 IFTKK301 pKa = 9.63 RR302 pKa = 11.84 AKK304 pKa = 10.1 KK305 pKa = 10.58 VFISAQMKK313 pKa = 10.09 GKK315 pKa = 10.31 SCIPGNKK322 pKa = 8.49 TGDD325 pKa = 3.5 YY326 pKa = 10.4 CQEE329 pKa = 4.06 KK330 pKa = 11.05 VNGCEE335 pKa = 3.92 KK336 pKa = 10.96 GCWNLPKK343 pKa = 10.49 SRR345 pKa = 11.84 TRR347 pKa = 11.84 QRR349 pKa = 11.84 RR350 pKa = 11.84 QSILFFWGLVEE361 pKa = 4.38 VKK363 pKa = 9.15 WGEE366 pKa = 3.97 GGEE369 pKa = 4.35 VYY371 pKa = 10.69 DD372 pKa = 6.09 DD373 pKa = 4.17 SVLKK377 pKa = 10.86 ALASQVDD384 pKa = 4.76 LNTASVKK391 pKa = 10.38 KK392 pKa = 10.34 LQLKK396 pKa = 10.5 LDD398 pKa = 4.34 LLSKK402 pKa = 10.52 AIWNGFCTNGTHH414 pKa = 6.7 SVQANMTITFKK425 pKa = 11.2 DD426 pKa = 3.19 GDD428 pKa = 4.19 EE429 pKa = 4.19 LVVLDD434 pKa = 4.36 WCNSDD439 pKa = 3.35 TLEE442 pKa = 4.72 AFIDD446 pKa = 3.8 SKK448 pKa = 11.16 SVQEE452 pKa = 4.18 HH453 pKa = 6.28 HH454 pKa = 7.19 LSDD457 pKa = 3.7 LVKK460 pKa = 10.29 VSIRR464 pKa = 11.84 MRR466 pKa = 11.84 IHH468 pKa = 5.68 QKK470 pKa = 10.14 RR471 pKa = 11.84 VTIATKK477 pKa = 10.33 LGCVLFGLIVCTAVLRR493 pKa = 11.84 ALWRR497 pKa = 11.84 RR498 pKa = 11.84 KK499 pKa = 10.03 DD500 pKa = 3.57 FSLSHH505 pKa = 5.13 YY506 pKa = 10.6 HH507 pKa = 7.17 EE508 pKa = 5.38 EE509 pKa = 4.24 DD510 pKa = 3.97 LNGQTCYY517 pKa = 10.18 SHH519 pKa = 6.78 HH520 pKa = 6.75 RR521 pKa = 11.84 FEE523 pKa = 4.46 KK524 pKa = 10.17 RR525 pKa = 11.84 YY526 pKa = 10.64 SRR528 pKa = 11.84 ACRR531 pKa = 11.84 CGNVVIGKK539 pKa = 9.69 NSFLKK544 pKa = 9.69 MAKK547 pKa = 9.87 CNNRR551 pKa = 11.84 SKK553 pKa = 11.26 LPDD556 pKa = 4.3 CLLMDD561 pKa = 5.72 DD562 pKa = 5.31 KK563 pKa = 11.54 PNEE566 pKa = 4.12 CDD568 pKa = 3.88 VLDD571 pKa = 4.58 FRR573 pKa = 11.84 QGQQKK578 pKa = 9.67 EE579 pKa = 4.67 VISEE583 pKa = 4.07 VQVIEE588 pKa = 4.22 FEE590 pKa = 4.13 IHH592 pKa = 6.02 GDD594 pKa = 3.44 EE595 pKa = 4.43 PRR597 pKa = 11.84 NEE599 pKa = 3.93 GSGAQTHH606 pKa = 5.77 QDD608 pKa = 3.01 LSEE611 pKa = 3.98 TPEE614 pKa = 4.03 RR615 pKa = 11.84 YY616 pKa = 9.66 EE617 pKa = 3.55 IAPLVFHH624 pKa = 6.48 NPEE627 pKa = 4.32 YY628 pKa = 10.08 VTQFHH633 pKa = 7.06 PKK635 pKa = 9.64
Molecular weight: 72.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.028
IPC2_protein 7.322
IPC_protein 7.102
Toseland 6.781
ProMoST 7.79
Dawson 7.819
Bjellqvist 8.331
Wikipedia 7.717
Rodwell 7.834
Grimsley 6.839
Solomon 7.834
Lehninger 7.863
Nozaki 8.682
DTASelect 7.995
Thurlkill 8.053
EMBOSS 8.039
Sillero 8.419
Patrickios 4.215
IPC_peptide 7.834
IPC2_peptide 7.497
IPC2.peptide.svr19 7.306
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3478
156
2092
869.5
99.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.112 ± 0.468
3.508 ± 0.503
5.98 ± 0.848
7.418 ± 0.881
3.652 ± 0.229
5.607 ± 0.399
2.53 ± 0.312
6.642 ± 0.095
7.849 ± 0.483
9.804 ± 0.598
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.537 ± 0.408
4.255 ± 0.28
3.853 ± 0.33
2.933 ± 0.419
6.009 ± 0.534
8.223 ± 0.516
4.974 ± 0.362
5.463 ± 0.453
1.438 ± 0.352
2.214 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here