Streptococcus satellite phage Javan544

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZQ57|A0A4D5ZQ57_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan544 OX=2558738 GN=JavanS544_0011 PE=4 SV=1
MM1 pKa = 7.86NLLQYY6 pKa = 10.83NNKK9 pKa = 9.31KK10 pKa = 10.49VSLTDD15 pKa = 3.29VEE17 pKa = 4.47GKK19 pKa = 9.2IWSGMAHH26 pKa = 5.76YY27 pKa = 10.36CDD29 pKa = 3.91AEE31 pKa = 4.51SYY33 pKa = 11.38DD34 pKa = 4.12EE35 pKa = 5.55DD36 pKa = 5.64EE37 pKa = 5.48DD38 pKa = 3.8MLDD41 pKa = 2.85IKK43 pKa = 10.96VGNEE47 pKa = 3.71YY48 pKa = 10.31IVFSEE53 pKa = 4.23SEE55 pKa = 4.08IYY57 pKa = 10.45SVEE60 pKa = 3.81ILNN63 pKa = 4.29

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZQF9|A0A4D5ZQF9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan544 OX=2558738 GN=JavanS544_0002 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 9.33ITEE5 pKa = 4.22VKK7 pKa = 9.97KK8 pKa = 10.98KK9 pKa = 10.66NGTTVYY15 pKa = 10.11RR16 pKa = 11.84ASVYY20 pKa = 10.81LGVDD24 pKa = 2.66KK25 pKa = 11.05VTGKK29 pKa = 10.21KK30 pKa = 10.24VKK32 pKa = 9.81TSITGRR38 pKa = 11.84TRR40 pKa = 11.84KK41 pKa = 8.72EE42 pKa = 4.07VKK44 pKa = 10.28SKK46 pKa = 10.57AQHH49 pKa = 5.62AQFDD53 pKa = 4.62FKK55 pKa = 11.17TNGSTVKK62 pKa = 9.6KK63 pKa = 7.67TVQVKK68 pKa = 8.4TYY70 pKa = 10.65KK71 pKa = 10.39EE72 pKa = 4.03LAALWLEE79 pKa = 4.23SHH81 pKa = 6.88KK82 pKa = 10.66LTVRR86 pKa = 11.84PQTFAVTRR94 pKa = 11.84RR95 pKa = 11.84QVNHH99 pKa = 6.25HH100 pKa = 7.38LIPEE104 pKa = 4.71FGSMKK109 pKa = 9.3IDD111 pKa = 4.12KK112 pKa = 8.35ITPSSIQEE120 pKa = 4.24FINNLAYY127 pKa = 9.97KK128 pKa = 10.09LVNFKK133 pKa = 10.28EE134 pKa = 4.15INSINRR140 pKa = 11.84RR141 pKa = 11.84ILQHH145 pKa = 6.43GVTLQLLTSNPGRR158 pKa = 11.84DD159 pKa = 3.17VILPKK164 pKa = 10.02KK165 pKa = 8.7QKK167 pKa = 9.97KK168 pKa = 9.27GRR170 pKa = 11.84EE171 pKa = 3.83AVKK174 pKa = 10.36FIEE177 pKa = 4.38PDD179 pKa = 3.26NLKK182 pKa = 10.56VFIDD186 pKa = 3.93YY187 pKa = 11.0AEE189 pKa = 4.3KK190 pKa = 10.86LSTKK194 pKa = 9.78SYY196 pKa = 7.56KK197 pKa = 9.42HH198 pKa = 5.68YY199 pKa = 10.78YY200 pKa = 9.1RR201 pKa = 11.84FVVFKK206 pKa = 10.82LLLATGCRR214 pKa = 11.84IGEE217 pKa = 4.1LAALEE222 pKa = 4.18WSDD225 pKa = 4.34IDD227 pKa = 5.1LKK229 pKa = 11.17EE230 pKa = 3.89KK231 pKa = 10.26TITISKK237 pKa = 9.01TYY239 pKa = 8.95SQEE242 pKa = 2.82IRR244 pKa = 11.84MIGEE248 pKa = 4.42TKK250 pKa = 9.99TKK252 pKa = 10.62AGTRR256 pKa = 11.84KK257 pKa = 9.72ISIDD261 pKa = 3.47PKK263 pKa = 7.3TTLMLRR269 pKa = 11.84QYY271 pKa = 9.79QNRR274 pKa = 11.84QRR276 pKa = 11.84LMFLEE281 pKa = 4.59VGAPAPSVVFATTVRR296 pKa = 11.84EE297 pKa = 3.82YY298 pKa = 11.13LPRR301 pKa = 11.84HH302 pKa = 5.71SLQQIIDD309 pKa = 3.93KK310 pKa = 10.56RR311 pKa = 11.84CEE313 pKa = 3.8TLGIPRR319 pKa = 11.84FTCHH323 pKa = 6.9AFRR326 pKa = 11.84HH327 pKa = 4.86THH329 pKa = 7.04ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.52RR346 pKa = 11.84LGHH349 pKa = 5.98SNISMTLDD357 pKa = 3.53VYY359 pKa = 11.49SHH361 pKa = 7.26LSQDD365 pKa = 3.5KK366 pKa = 9.15EE367 pKa = 4.33KK368 pKa = 10.8EE369 pKa = 3.96AVSYY373 pKa = 9.5YY374 pKa = 10.62EE375 pKa = 4.12KK376 pKa = 10.7AINSLL381 pKa = 3.57

Molecular weight: 43.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

2976

37

549

156.6

17.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.821 ± 0.546

0.874 ± 0.128

5.41 ± 0.4

8.233 ± 0.684

4.234 ± 0.624

5.309 ± 0.459

1.378 ± 0.292

6.586 ± 0.426

9.711 ± 0.684

10.249 ± 0.403

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.339

4.805 ± 0.453

2.957 ± 0.444

4.637 ± 0.399

4.671 ± 0.386

5.914 ± 0.458

6.183 ± 0.501

4.872 ± 0.476

0.874 ± 0.165

3.797 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski