Haemophilus phage HP1 (strain HP1c1) (Bacteriophage HP1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Hpunavirus; Haemophilus virus HP1

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P51724|YO22_BPHC1 Uncharacterized 26.0 kDa protein in rep-hol intergenic region OS=Haemophilus phage HP1 (strain HP1c1) OX=1289570 PE=4 SV=1
MM1 pKa = 7.14ATVKK5 pKa = 10.63KK6 pKa = 9.83MLYY9 pKa = 8.92QQLTAFLLTKK19 pKa = 10.33LPKK22 pKa = 9.49RR23 pKa = 11.84YY24 pKa = 9.56HH25 pKa = 6.21GNFYY29 pKa = 10.93SWIEE33 pKa = 3.96DD34 pKa = 3.07GKK36 pKa = 11.04LLNEE40 pKa = 4.22GRR42 pKa = 11.84QVTEE46 pKa = 3.93NGIEE50 pKa = 4.2VCHH53 pKa = 6.73LSYY56 pKa = 11.3NGVFHH61 pKa = 7.48FEE63 pKa = 3.71ALPFNEE69 pKa = 4.4ISPAYY74 pKa = 10.22LMAHH78 pKa = 5.4IQVWVNEE85 pKa = 4.32NDD87 pKa = 3.42PMRR90 pKa = 11.84DD91 pKa = 3.31VLDD94 pKa = 4.02EE95 pKa = 4.34SEE97 pKa = 4.82IPFDD101 pKa = 5.72LDD103 pKa = 4.41IIDD106 pKa = 5.96DD107 pKa = 4.04NTTDD111 pKa = 5.25LIFTIAFRR119 pKa = 11.84EE120 pKa = 4.28PLTAMEE126 pKa = 4.97DD127 pKa = 3.61NEE129 pKa = 4.78GEE131 pKa = 4.18LKK133 pKa = 10.14IDD135 pKa = 3.54GVNYY139 pKa = 10.23RR140 pKa = 11.84LDD142 pKa = 4.12EE143 pKa = 4.59IEE145 pKa = 4.36VFTAEE150 pKa = 4.36EE151 pKa = 3.83IDD153 pKa = 3.67VVVRR157 pKa = 11.84VEE159 pKa = 4.22HH160 pKa = 5.74EE161 pKa = 4.37HH162 pKa = 6.79PNGDD166 pKa = 3.3

Molecular weight: 19.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P51702|YO03_BPHC1 Uncharacterized 8.9 kDa protein in int-C1 intergenic region OS=Haemophilus phage HP1 (strain HP1c1) OX=1289570 PE=4 SV=1
MM1 pKa = 7.77LINVSIKK8 pKa = 10.38LIKK11 pKa = 10.12LYY13 pKa = 10.59QRR15 pKa = 11.84LAPQKK20 pKa = 10.41IRR22 pKa = 11.84DD23 pKa = 3.55ACRR26 pKa = 11.84FEE28 pKa = 4.46PTCSNYY34 pKa = 10.73AILALQKK41 pKa = 10.32YY42 pKa = 7.18GFWKK46 pKa = 8.44GWKK49 pKa = 8.41MALNRR54 pKa = 11.84LGRR57 pKa = 11.84CKK59 pKa = 10.52YY60 pKa = 10.14PNGGEE65 pKa = 4.08DD66 pKa = 3.73LPP68 pKa = 5.5

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

9882

61

925

241.0

27.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.085 ± 0.422

0.901 ± 0.134

5.505 ± 0.216

6.831 ± 0.429

4.382 ± 0.193

6.234 ± 0.462

1.923 ± 0.166

6.426 ± 0.275

7.772 ± 0.363

8.885 ± 0.362

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.179

5.839 ± 0.322

3.177 ± 0.203

4.685 ± 0.255

4.655 ± 0.353

6.173 ± 0.237

5.606 ± 0.311

5.97 ± 0.24

1.305 ± 0.116

3.441 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski