Modestobacter caceresii
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3847 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A098Y9H3|A0A098Y9H3_9ACTN Methylase OS=Modestobacter caceresii OX=1522368 GN=IN07_08945 PE=4 SV=1
MM1 pKa = 7.35 NSVSRR6 pKa = 11.84 SGRR9 pKa = 11.84 RR10 pKa = 11.84 ALVATAGITATALALSACGGDD31 pKa = 4.16 DD32 pKa = 3.7 GDD34 pKa = 4.7 GGGSGGGDD42 pKa = 3.33 SLIIGTTDD50 pKa = 3.59 KK51 pKa = 10.43 ITTIDD56 pKa = 3.49 PAGSYY61 pKa = 11.17 DD62 pKa = 3.54 NGSFAVMNQVYY73 pKa = 8.97 PFLMNTPLGSPDD85 pKa = 3.76 VEE87 pKa = 3.9 PDD89 pKa = 3.01 IAEE92 pKa = 4.25 SAEE95 pKa = 4.09 FTSPTEE101 pKa = 3.89 YY102 pKa = 10.56 TVTLKK107 pKa = 11.03 DD108 pKa = 3.31 GLTFANGNEE117 pKa = 4.04 LTASDD122 pKa = 4.03 VKK124 pKa = 10.89 FSFDD128 pKa = 3.5 RR129 pKa = 11.84 MVAINDD135 pKa = 3.42 EE136 pKa = 4.45 SGPASLLYY144 pKa = 10.33 NLEE147 pKa = 4.04 STEE150 pKa = 4.16 VVDD153 pKa = 3.97 DD154 pKa = 3.79 TTVVFNLKK162 pKa = 10.62 SPDD165 pKa = 3.66 DD166 pKa = 3.65 QVFPQILSSPAGPIVDD182 pKa = 3.9 EE183 pKa = 5.08 DD184 pKa = 4.14 VFAADD189 pKa = 5.75 ALTTDD194 pKa = 3.78 QEE196 pKa = 4.56 IVDD199 pKa = 4.23 GNAFAGPYY207 pKa = 10.29 AITNYY212 pKa = 10.11 DD213 pKa = 3.34 QNNLVSYY220 pKa = 9.39 EE221 pKa = 4.0 AFEE224 pKa = 5.05 GYY226 pKa = 9.63 QGLLGAPEE234 pKa = 4.23 TEE236 pKa = 4.55 TVNVRR241 pKa = 11.84 YY242 pKa = 9.58 YY243 pKa = 11.63 ADD245 pKa = 3.67 ASNLKK250 pKa = 10.44 LDD252 pKa = 3.86 VQEE255 pKa = 4.86 GNVDD259 pKa = 3.42 VAFRR263 pKa = 11.84 SLTATDD269 pKa = 3.86 VEE271 pKa = 4.73 DD272 pKa = 4.47 LRR274 pKa = 11.84 GDD276 pKa = 3.56 DD277 pKa = 3.67 SVNVVDD283 pKa = 5.57 GPGGEE288 pKa = 3.65 IRR290 pKa = 11.84 YY291 pKa = 8.28 ITFNFNTQPYY301 pKa = 9.66 GATTPEE307 pKa = 4.22 ADD309 pKa = 3.52 PAKK312 pKa = 10.57 ALAVRR317 pKa = 11.84 QAAAHH322 pKa = 6.45 LIDD325 pKa = 4.53 RR326 pKa = 11.84 EE327 pKa = 4.06 EE328 pKa = 4.85 LADD331 pKa = 3.59 QVYY334 pKa = 10.44 KK335 pKa = 10.17 GTYY338 pKa = 7.53 TPLYY342 pKa = 9.34 SYY344 pKa = 10.97 VPEE347 pKa = 4.54 GLTGAVEE354 pKa = 4.13 PLLEE358 pKa = 4.61 KK359 pKa = 11.22 YY360 pKa = 10.72 GDD362 pKa = 3.78 GSGAPDD368 pKa = 3.58 AEE370 pKa = 4.26 AAAQVLQAAGVQTPVEE386 pKa = 4.26 LNLQYY391 pKa = 11.47 SNDD394 pKa = 3.55 HH395 pKa = 6.32 YY396 pKa = 11.31 GPSSGDD402 pKa = 3.07 EE403 pKa = 3.82 YY404 pKa = 11.92 ALIKK408 pKa = 10.69 DD409 pKa = 3.71 QLEE412 pKa = 4.2 SSGLFTVNLQTTEE425 pKa = 3.68 WVQYY429 pKa = 10.88 SEE431 pKa = 5.65 DD432 pKa = 3.69 RR433 pKa = 11.84 TADD436 pKa = 3.37 VYY438 pKa = 10.68 PAYY441 pKa = 10.26 QLGWFPDD448 pKa = 3.67 YY449 pKa = 11.48 SDD451 pKa = 4.11 ADD453 pKa = 3.57 NYY455 pKa = 8.96 LTPFFLTEE463 pKa = 3.86 NFLGNHH469 pKa = 5.69 YY470 pKa = 10.65 SDD472 pKa = 5.11 PEE474 pKa = 4.26 VNDD477 pKa = 5.67 LILQQATTTDD487 pKa = 3.61 PAEE490 pKa = 4.2 RR491 pKa = 11.84 EE492 pKa = 3.98 ALIEE496 pKa = 4.02 QIQGQVADD504 pKa = 4.97 DD505 pKa = 4.92 LSTLPYY511 pKa = 10.41 LQGAQVAVTGTDD523 pKa = 3.25 VQGAEE528 pKa = 4.25 DD529 pKa = 3.78 TLDD532 pKa = 3.46 PSFKK536 pKa = 10.15 FRR538 pKa = 11.84 YY539 pKa = 9.04 GALSKK544 pKa = 11.17 GG545 pKa = 3.56
Molecular weight: 58.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.681
IPC_protein 3.706
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.63
Rodwell 3.528
Grimsley 3.389
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.05
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.834
Patrickios 0.846
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A098YDQ2|A0A098YDQ2_9ACTN Bifunctional purine biosynthesis protein PurH OS=Modestobacter caceresii OX=1522368 GN=purH PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 8.98 RR4 pKa = 11.84 TFQPNTRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.83 THH17 pKa = 5.18 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.23 GRR40 pKa = 11.84 NKK42 pKa = 10.44 LSAA45 pKa = 3.84
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3847
0
3847
1186502
37
2005
308.4
32.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.24 ± 0.066
0.666 ± 0.01
6.286 ± 0.034
5.636 ± 0.031
2.563 ± 0.023
9.522 ± 0.031
2.037 ± 0.021
2.83 ± 0.034
1.424 ± 0.026
10.797 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.648 ± 0.015
1.471 ± 0.019
6.03 ± 0.033
2.904 ± 0.02
7.839 ± 0.045
4.891 ± 0.024
6.128 ± 0.027
9.86 ± 0.038
1.484 ± 0.018
1.746 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here