Human immunodeficiency virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Primate lentivirus group; unclassified Primate lentivirus group

Average proteome isoelectric point is 7.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8EEZ4|W8EEZ4_9PLVG Gag polyprotein OS=Human immunodeficiency virus OX=12721 GN=gag PE=3 SV=1
MM1 pKa = 7.55TPLQLSAIQIGAIVGLIVALILAIVVWTIVAIEE34 pKa = 4.01VKK36 pKa = 10.48KK37 pKa = 10.53ILKK40 pKa = 8.56QRR42 pKa = 11.84KK43 pKa = 7.89IDD45 pKa = 3.64KK46 pKa = 10.38LVDD49 pKa = 4.57RR50 pKa = 11.84IRR52 pKa = 11.84QRR54 pKa = 11.84AEE56 pKa = 3.46DD57 pKa = 3.85SGNEE61 pKa = 4.17SEE63 pKa = 5.59GDD65 pKa = 3.29TDD67 pKa = 4.04EE68 pKa = 4.39LAKK71 pKa = 10.51LVEE74 pKa = 4.5MGGFDD79 pKa = 3.68PWVADD84 pKa = 3.64NLL86 pKa = 4.12

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8EG24|W8EG24_9PLVG Protein Tat OS=Human immunodeficiency virus OX=12721 GN=tat PE=3 SV=1
MM1 pKa = 7.85AGRR4 pKa = 11.84SGGTDD9 pKa = 3.24EE10 pKa = 5.4DD11 pKa = 3.92LLRR14 pKa = 11.84AVRR17 pKa = 11.84IIKK20 pKa = 9.75ILYY23 pKa = 7.91QNPPPSSEE31 pKa = 3.84GTRR34 pKa = 11.84QARR37 pKa = 11.84KK38 pKa = 8.29NRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84WRR45 pKa = 11.84ARR47 pKa = 11.84QRR49 pKa = 11.84QIRR52 pKa = 11.84ALSDD56 pKa = 3.92RR57 pKa = 11.84ILIPFLGGSTEE68 pKa = 4.02PVPLQLPPLEE78 pKa = 5.3RR79 pKa = 11.84LTLDD83 pKa = 3.68CSXDD87 pKa = 3.91CGTSGTQGVGSPQILVEE104 pKa = 4.27SPEE107 pKa = 4.06ILDD110 pKa = 4.21SGTKK114 pKa = 8.81EE115 pKa = 3.72

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

3149

86

1000

349.9

39.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.938 ± 0.35

1.937 ± 0.399

4.351 ± 0.388

6.701 ± 0.566

2.668 ± 0.277

7.304 ± 0.274

2.286 ± 0.482

7.113 ± 0.803

7.145 ± 0.99

8.32 ± 0.556

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.842 ± 0.341

4.573 ± 0.826

5.557 ± 0.675

5.526 ± 0.477

5.907 ± 0.826

5.367 ± 0.486

6.097 ± 0.578

5.938 ± 0.447

2.858 ± 0.302

2.413 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski