Porcine circovirus
Average proteome isoelectric point is 8.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M1LLC3|M1LLC3_9CIRC Truncated Rep protein OS=Porcine circovirus OX=46221 GN=rep PE=4 SV=1
MM1 pKa = 7.74 PSKK4 pKa = 10.67 KK5 pKa = 9.86 SGRR8 pKa = 11.84 SGPQPHH14 pKa = 6.51 KK15 pKa = 10.37 RR16 pKa = 11.84 WVFTLNNPSEE26 pKa = 4.33 DD27 pKa = 3.36 EE28 pKa = 3.99 RR29 pKa = 11.84 KK30 pKa = 10.05 KK31 pKa = 10.56 IRR33 pKa = 11.84 EE34 pKa = 4.09 LPISLFDD41 pKa = 3.56 YY42 pKa = 9.9 FIVGEE47 pKa = 4.19 EE48 pKa = 4.25 GNEE51 pKa = 4.12 EE52 pKa = 3.98 GRR54 pKa = 11.84 TPHH57 pKa = 6.16 LQGFANFVKK66 pKa = 10.4 KK67 pKa = 7.73 QTFNKK72 pKa = 8.58 VKK74 pKa = 8.5 WYY76 pKa = 10.26 FGARR80 pKa = 11.84 CHH82 pKa = 6.85 IEE84 pKa = 3.87 KK85 pKa = 10.86 AKK87 pKa = 9.75 GTDD90 pKa = 3.47 QQNKK94 pKa = 8.81 EE95 pKa = 4.19 YY96 pKa = 10.41 CSKK99 pKa = 10.8 EE100 pKa = 3.71 GNLLIEE106 pKa = 4.81 CGAPRR111 pKa = 11.84 SQGQRR116 pKa = 11.84 SDD118 pKa = 3.49 LSTAVSTLLEE128 pKa = 4.52 SGSLVTVAEE137 pKa = 4.06 QHH139 pKa = 5.99 PVTFVRR145 pKa = 11.84 NFRR148 pKa = 11.84 GLAEE152 pKa = 4.1 LLKK155 pKa = 11.06 VSGKK159 pKa = 7.43 MHH161 pKa = 6.06 QEE163 pKa = 3.46 WW164 pKa = 3.41
Molecular weight: 18.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.845
IPC2_protein 8.521
IPC_protein 8.478
Toseland 9.355
ProMoST 9.004
Dawson 9.545
Bjellqvist 9.297
Wikipedia 9.648
Rodwell 9.984
Grimsley 9.56
Solomon 9.648
Lehninger 9.633
Nozaki 9.56
DTASelect 9.209
Thurlkill 9.443
EMBOSS 9.765
Sillero 9.56
Patrickios 7.644
IPC_peptide 9.648
IPC2_peptide 7.922
IPC2.peptide.svr19 7.801
Protein with the highest isoelectric point:
>tr|M1LLC3|M1LLC3_9CIRC Truncated Rep protein OS=Porcine circovirus OX=46221 GN=rep PE=4 SV=1
MM1 pKa = 7.74 PSKK4 pKa = 10.67 KK5 pKa = 9.86 SGRR8 pKa = 11.84 SGPQPHH14 pKa = 6.51 KK15 pKa = 10.37 RR16 pKa = 11.84 WVFTLNNPSEE26 pKa = 4.33 DD27 pKa = 3.36 EE28 pKa = 3.99 RR29 pKa = 11.84 KK30 pKa = 10.05 KK31 pKa = 10.56 IRR33 pKa = 11.84 EE34 pKa = 4.09 LPISLFDD41 pKa = 3.56 YY42 pKa = 9.9 FIVGEE47 pKa = 4.19 EE48 pKa = 4.25 GNEE51 pKa = 4.12 EE52 pKa = 3.98 GRR54 pKa = 11.84 TPHH57 pKa = 6.16 LQGFANFVKK66 pKa = 10.4 KK67 pKa = 7.73 QTFNKK72 pKa = 8.58 VKK74 pKa = 8.5 WYY76 pKa = 10.26 FGARR80 pKa = 11.84 CHH82 pKa = 6.85 IEE84 pKa = 3.87 KK85 pKa = 10.86 AKK87 pKa = 9.75 GTDD90 pKa = 3.47 QQNKK94 pKa = 8.81 EE95 pKa = 4.19 YY96 pKa = 10.41 CSKK99 pKa = 10.8 EE100 pKa = 3.71 GNLLIEE106 pKa = 4.81 CGAPRR111 pKa = 11.84 SQGQRR116 pKa = 11.84 SDD118 pKa = 3.49 LSTAVSTLLEE128 pKa = 4.52 SGSLVTVAEE137 pKa = 4.06 QHH139 pKa = 5.99 PVTFVRR145 pKa = 11.84 NFRR148 pKa = 11.84 GLAEE152 pKa = 4.1 LLKK155 pKa = 11.06 VSGKK159 pKa = 7.43 MHH161 pKa = 6.06 QEE163 pKa = 3.46 WW164 pKa = 3.41
Molecular weight: 18.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.845
IPC2_protein 8.521
IPC_protein 8.478
Toseland 9.355
ProMoST 9.004
Dawson 9.545
Bjellqvist 9.297
Wikipedia 9.648
Rodwell 9.984
Grimsley 9.56
Solomon 9.648
Lehninger 9.633
Nozaki 9.56
DTASelect 9.209
Thurlkill 9.443
EMBOSS 9.765
Sillero 9.56
Patrickios 7.644
IPC_peptide 9.648
IPC2_peptide 7.922
IPC2.peptide.svr19 7.801
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
164
164
164
164.0
18.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.268 ± 0.0
1.829 ± 0.0
2.439 ± 0.0
9.146 ± 0.0
5.488 ± 0.0
8.537 ± 0.0
3.049 ± 0.0
3.049 ± 0.0
9.146 ± 0.0
7.927 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.22 ± 0.0
4.878 ± 0.0
4.878 ± 0.0
5.488 ± 0.0
6.098 ± 0.0
7.927 ± 0.0
4.878 ± 0.0
6.098 ± 0.0
1.829 ± 0.0
1.829 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here