Massilimicrobiota sp. An134

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Massilimicrobiota; unclassified Massilimicrobiota

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2537 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4S6Q7|A0A1Y4S6Q7_9FIRM Bacitracin ABC transporter ATP-binding protein OS=Massilimicrobiota sp. An134 OX=1965557 GN=B5E79_11915 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.19KK3 pKa = 9.88IKK5 pKa = 10.38CLLFVFCVCLLSACQSNDD23 pKa = 2.74NVSKK27 pKa = 11.11VNEE30 pKa = 4.08QQSVSVPNEE39 pKa = 4.16TISSDD44 pKa = 3.28GKK46 pKa = 10.59ILIAYY51 pKa = 7.36FTWADD56 pKa = 3.55NTHH59 pKa = 5.99VDD61 pKa = 4.24DD62 pKa = 5.09PSSVDD67 pKa = 3.15VDD69 pKa = 3.61ASTSASVLAPGNTEE83 pKa = 3.8KK84 pKa = 10.72VAHH87 pKa = 7.01FIQEE91 pKa = 4.15QVGGDD96 pKa = 3.59MFSIIVDD103 pKa = 3.62EE104 pKa = 5.38PYY106 pKa = 10.91SSDD109 pKa = 3.45YY110 pKa = 11.41DD111 pKa = 3.61EE112 pKa = 5.44CLDD115 pKa = 4.07RR116 pKa = 11.84ASDD119 pKa = 3.83EE120 pKa = 4.36PAQQARR126 pKa = 11.84PIIVDD131 pKa = 3.37TVEE134 pKa = 5.14NMDD137 pKa = 3.76EE138 pKa = 4.2YY139 pKa = 11.69DD140 pKa = 4.25VIFLGYY146 pKa = 8.36PNWWSSCPMAIFSFIEE162 pKa = 4.6SYY164 pKa = 11.11DD165 pKa = 3.78LSNKK169 pKa = 9.24TIIPFCAHH177 pKa = 5.41GTGGLGRR184 pKa = 11.84SIEE187 pKa = 4.3DD188 pKa = 3.49LQDD191 pKa = 3.96ALPHH195 pKa = 5.64STLLDD200 pKa = 3.41AFGVYY205 pKa = 9.98RR206 pKa = 11.84PDD208 pKa = 3.42TDD210 pKa = 3.6SCQDD214 pKa = 5.12DD215 pKa = 4.01VNQWLSDD222 pKa = 3.0IGMVDD227 pKa = 3.08

Molecular weight: 25.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4SJ66|A0A1Y4SJ66_9FIRM Cysteine--tRNA ligase OS=Massilimicrobiota sp. An134 OX=1965557 GN=cysS PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.51RR10 pKa = 11.84KK11 pKa = 9.77RR12 pKa = 11.84SKK14 pKa = 8.62THH16 pKa = 5.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.02RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.93VLSAA44 pKa = 4.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2537

0

2537

746771

27

2433

294.4

33.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.571 ± 0.054

1.418 ± 0.021

6.06 ± 0.037

6.749 ± 0.043

4.447 ± 0.041

5.85 ± 0.054

2.618 ± 0.033

8.887 ± 0.058

7.422 ± 0.044

9.382 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.104 ± 0.025

4.812 ± 0.041

2.917 ± 0.024

4.516 ± 0.044

3.427 ± 0.033

5.798 ± 0.034

5.023 ± 0.047

6.315 ± 0.046

0.729 ± 0.014

4.953 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski