Tortoise microvirus 98
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W706|A0A4P8W706_9VIRU Uncharacterized protein OS=Tortoise microvirus 98 OX=2583207 PE=4 SV=1
MM1 pKa = 7.64 EE2 pKa = 5.5 SFMFNIDD9 pKa = 3.78 LQNGSIIDD17 pKa = 4.24 SINTPLAAVQLLIPCAMTRR36 pKa = 11.84 CIDD39 pKa = 3.62 KK40 pKa = 9.26 LTEE43 pKa = 3.81 RR44 pKa = 11.84 ANISLADD51 pKa = 4.73 FIRR54 pKa = 11.84 LACAEE59 pKa = 4.54 YY60 pKa = 10.39 ILSFKK65 pKa = 10.79 DD66 pKa = 3.18
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.462
IPC2_protein 4.558
IPC_protein 4.317
Toseland 4.139
ProMoST 4.291
Dawson 4.304
Bjellqvist 4.558
Wikipedia 4.24
Rodwell 4.151
Grimsley 4.05
Solomon 4.279
Lehninger 4.24
Nozaki 4.431
DTASelect 4.635
Thurlkill 4.19
EMBOSS 4.24
Sillero 4.431
Patrickios 3.656
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.422
Protein with the highest isoelectric point:
>tr|A0A4P8W7Q4|A0A4P8W7Q4_9VIRU DNA pilot protein OS=Tortoise microvirus 98 OX=2583207 PE=4 SV=1
MM1 pKa = 7.26 TFSISLQPLNMMRR14 pKa = 11.84 WRR16 pKa = 11.84 MSDD19 pKa = 3.23 MSCSNPLTINVRR31 pKa = 11.84 GRR33 pKa = 11.84 AVSVPCRR40 pKa = 11.84 YY41 pKa = 10.15 CMSCRR46 pKa = 11.84 TEE48 pKa = 4.02 YY49 pKa = 10.73 QSALLFGAKK58 pKa = 9.68 HH59 pKa = 5.83 EE60 pKa = 5.09 LFDD63 pKa = 4.85 CYY65 pKa = 10.42 RR66 pKa = 11.84 AGLGASFVTLTYY78 pKa = 10.57 SDD80 pKa = 3.54 QYY82 pKa = 11.4 LPEE85 pKa = 4.8 NGSLRR90 pKa = 11.84 KK91 pKa = 9.53 KK92 pKa = 10.45 DD93 pKa = 3.61 VQNLLKK99 pKa = 10.51 NVRR102 pKa = 11.84 IQAKK106 pKa = 9.2 RR107 pKa = 11.84 KK108 pKa = 7.69 TGIPPFKK115 pKa = 10.58 YY116 pKa = 10.17 IMCGEE121 pKa = 4.19 YY122 pKa = 10.4 GDD124 pKa = 3.89 KK125 pKa = 10.42 FGRR128 pKa = 11.84 AHH130 pKa = 4.73 YY131 pKa = 9.7 HH132 pKa = 5.15 IVFIGLSDD140 pKa = 3.74 VLVHH144 pKa = 6.66 NFVKK148 pKa = 10.37 PLWKK152 pKa = 10.38 FGLIDD157 pKa = 3.52 IGALRR162 pKa = 11.84 EE163 pKa = 4.12 GGLRR167 pKa = 11.84 YY168 pKa = 9.55 ALKK171 pKa = 10.43 YY172 pKa = 7.66 CTKK175 pKa = 10.26 AARR178 pKa = 11.84 GKK180 pKa = 10.15 KK181 pKa = 10.14 AEE183 pKa = 4.17 EE184 pKa = 4.73 LYY186 pKa = 11.03 DD187 pKa = 3.73 NQDD190 pKa = 2.61 IEE192 pKa = 5.31 RR193 pKa = 11.84 PFICHH198 pKa = 5.72 SRR200 pKa = 11.84 GIGKK204 pKa = 8.56 NWFIEE209 pKa = 4.11 NADD212 pKa = 4.83 DD213 pKa = 5.24 IIAHH217 pKa = 6.03 NFTYY221 pKa = 10.71 LEE223 pKa = 4.03 NGIRR227 pKa = 11.84 KK228 pKa = 8.25 PLPAYY233 pKa = 10.08 FRR235 pKa = 11.84 RR236 pKa = 11.84 QYY238 pKa = 11.51 DD239 pKa = 3.23 AFKK242 pKa = 10.37 TFDD245 pKa = 3.1 VSEE248 pKa = 4.62 AIKK251 pKa = 10.75 YY252 pKa = 10.27 LSEE255 pKa = 3.56 EE256 pKa = 4.27 AKK258 pKa = 9.98 IHH260 pKa = 6.75 GYY262 pKa = 10.66 DD263 pKa = 5.37 DD264 pKa = 3.39 YY265 pKa = 11.85 RR266 pKa = 11.84 DD267 pKa = 3.22 WSKK270 pKa = 11.28 QKK272 pKa = 9.49 TYY274 pKa = 7.89 QTEE277 pKa = 4.29 KK278 pKa = 10.35 EE279 pKa = 3.92 LTAAARR285 pKa = 11.84 ASGIPVDD292 pKa = 4.47 DD293 pKa = 3.67 SSFRR297 pKa = 11.84 LQSGFTANNHH307 pKa = 5.5 SPLVNTLLAPRR318 pKa = 11.84 RR319 pKa = 11.84 EE320 pKa = 3.94 ATTNFKK326 pKa = 10.31 EE327 pKa = 4.17
Molecular weight: 37.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.637
IPC2_protein 8.551
IPC_protein 8.448
Toseland 8.887
ProMoST 8.916
Dawson 9.297
Bjellqvist 9.224
Wikipedia 9.458
Rodwell 9.414
Grimsley 9.297
Solomon 9.355
Lehninger 9.311
Nozaki 9.311
DTASelect 9.107
Thurlkill 9.18
EMBOSS 9.414
Sillero 9.37
Patrickios 4.393
IPC_peptide 9.341
IPC2_peptide 8.053
IPC2.peptide.svr19 7.75
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1655
66
583
236.4
26.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.459 ± 0.54
1.269 ± 0.489
6.224 ± 0.222
4.894 ± 0.646
4.592 ± 0.557
5.801 ± 0.588
1.39 ± 0.276
5.74 ± 0.576
4.23 ± 1.04
8.882 ± 0.819
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.142 ± 0.292
5.559 ± 0.589
4.713 ± 0.667
3.565 ± 0.972
6.042 ± 0.291
8.157 ± 0.531
6.405 ± 0.514
5.378 ± 0.735
0.906 ± 0.247
4.653 ± 0.709
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here