Erysipelothrix sp. HDW6A
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1961 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G7WWU2|A0A6G7WWU2_9FIRM Uncharacterized protein OS=Erysipelothrix sp. HDW6A OX=2714928 GN=G7059_01810 PE=4 SV=1
MM1 pKa = 7.65 AGKK4 pKa = 9.87 QKK6 pKa = 10.45 FCEE9 pKa = 4.42 CEE11 pKa = 4.27 SPSSVYY17 pKa = 10.58 TEE19 pKa = 4.28 VVANDD24 pKa = 3.8 TAHH27 pKa = 6.89 VDD29 pKa = 3.61 YY30 pKa = 11.23 CSDD33 pKa = 3.73 CNLPIEE39 pKa = 4.4 DD40 pKa = 3.6 TFEE43 pKa = 3.84 YY44 pKa = 9.84 FQYY47 pKa = 10.98 GSEE50 pKa = 4.25 DD51 pKa = 4.06 YY52 pKa = 11.24 YY53 pKa = 11.89 GG54 pKa = 3.56
Molecular weight: 6.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.917
IPC2_protein 3.872
IPC_protein 3.732
Toseland 3.554
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.478
Solomon 3.681
Lehninger 3.63
Nozaki 3.859
DTASelect 3.999
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.846
Patrickios 0.006
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.775
Protein with the highest isoelectric point:
>tr|A0A6G7WWU7|A0A6G7WWU7_9FIRM tRNA-specific adenosine deaminase OS=Erysipelothrix sp. HDW6A OX=2714928 GN=tadA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSKK9 pKa = 9.95 RR10 pKa = 11.84 KK11 pKa = 7.81 HH12 pKa = 5.11 QKK14 pKa = 8.48 VHH16 pKa = 5.53 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 8.28 TVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.59 GRR39 pKa = 11.84 KK40 pKa = 8.44 VLSAA44 pKa = 4.05
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1961
0
1961
589313
25
2195
300.5
33.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.055 ± 0.059
0.665 ± 0.015
6.053 ± 0.045
7.153 ± 0.063
4.519 ± 0.041
6.154 ± 0.051
1.938 ± 0.028
8.53 ± 0.056
6.711 ± 0.05
9.345 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.762 ± 0.03
5.293 ± 0.049
3.052 ± 0.034
3.232 ± 0.032
3.902 ± 0.044
6.672 ± 0.046
5.604 ± 0.054
7.369 ± 0.048
0.755 ± 0.018
4.235 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here