Lactococcus virus KSY1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Chopinvirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6MAH6|A6MAH6_9CAUD Gp111 OS=Lactococcus virus KSY1 OX=388452 GN=KSY1p111 PE=4 SV=1
MM1 pKa = 8.3DD2 pKa = 3.86EE3 pKa = 4.07QKK5 pKa = 10.83LFKK8 pKa = 10.12TLQSLFADD16 pKa = 4.94FNIDD20 pKa = 3.46CCDD23 pKa = 3.27EE24 pKa = 4.75SEE26 pKa = 4.1VHH28 pKa = 6.84EE29 pKa = 4.76IYY31 pKa = 10.99EE32 pKa = 4.78EE33 pKa = 3.9IDD35 pKa = 3.25KK36 pKa = 11.3AAGTSLADD44 pKa = 4.0IYY46 pKa = 11.15RR47 pKa = 11.84KK48 pKa = 10.58DD49 pKa = 3.7YY50 pKa = 10.23MDD52 pKa = 4.16EE53 pKa = 4.46DD54 pKa = 4.01EE55 pKa = 5.44EE56 pKa = 4.73EE57 pKa = 4.74DD58 pKa = 4.33GEE60 pKa = 4.41EE61 pKa = 3.95

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6MA73|A6MA73_9CAUD Gp009 OS=Lactococcus virus KSY1 OX=388452 GN=KSY1p009 PE=4 SV=1
MM1 pKa = 7.16YY2 pKa = 10.54KK3 pKa = 8.74NTRR6 pKa = 11.84EE7 pKa = 4.03LSPEE11 pKa = 4.03IIALVNDD18 pKa = 3.9NFNTYY23 pKa = 10.52YY24 pKa = 11.56NMLISDD30 pKa = 4.32NKK32 pKa = 9.47YY33 pKa = 9.18STISRR38 pKa = 11.84IKK40 pKa = 10.47KK41 pKa = 8.12NAPFAWSLLSEE52 pKa = 4.27GQKK55 pKa = 8.73TRR57 pKa = 11.84LYY59 pKa = 9.47RR60 pKa = 11.84TAKK63 pKa = 9.39KK64 pKa = 9.69HH65 pKa = 6.22RR66 pKa = 11.84KK67 pKa = 7.97FF68 pKa = 3.58

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

130

0

130

24643

31

1437

189.6

21.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.432 ± 0.314

0.812 ± 0.068

6.813 ± 0.167

6.399 ± 0.284

3.827 ± 0.152

6.115 ± 0.245

1.769 ± 0.112

7.02 ± 0.206

7.256 ± 0.295

7.799 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.93 ± 0.108

6.253 ± 0.21

3.502 ± 0.125

3.506 ± 0.187

3.689 ± 0.117

7.272 ± 0.234

6.505 ± 0.187

6.379 ± 0.174

1.201 ± 0.079

4.521 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski