Penicillium chrysogenum virus (isolate Caston/2003) (PcV)
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q8JVC0|P3_PCVC Uncharacterized protein p3 OS=Penicillium chrysogenum virus (isolate Caston/2003) OX=654932 GN=p3 PE=4 SV=1
MM1 pKa = 7.24 AAEE4 pKa = 4.4 ARR6 pKa = 11.84 VIDD9 pKa = 4.24 YY10 pKa = 8.19 MKK12 pKa = 10.71 GLPQSYY18 pKa = 6.77 QHH20 pKa = 6.8 RR21 pKa = 11.84 EE22 pKa = 3.69 TYY24 pKa = 10.4 FEE26 pKa = 4.74 QFLEE30 pKa = 4.34 QNSEE34 pKa = 4.16 RR35 pKa = 11.84 LVEE38 pKa = 4.75 EE39 pKa = 3.44 IDD41 pKa = 3.91 GPVVEE46 pKa = 5.52 KK47 pKa = 11.11 GLISDD52 pKa = 4.37 GEE54 pKa = 4.23 KK55 pKa = 10.56 EE56 pKa = 4.42 IIRR59 pKa = 11.84 HH60 pKa = 5.96 LNVQDD65 pKa = 3.46 KK66 pKa = 8.36 FTINGFRR73 pKa = 11.84 KK74 pKa = 9.47 SVKK77 pKa = 8.0 ICHH80 pKa = 5.5 MVSLGATRR88 pKa = 11.84 QTKK91 pKa = 8.55 RR92 pKa = 11.84 TGTTEE97 pKa = 4.22 LDD99 pKa = 3.6 FMGAGPWTLYY109 pKa = 10.07 PSSARR114 pKa = 11.84 ALYY117 pKa = 10.02 SSKK120 pKa = 10.59 EE121 pKa = 4.27 STVHH125 pKa = 6.82 LVDD128 pKa = 3.38 EE129 pKa = 4.7 AQRR132 pKa = 11.84 RR133 pKa = 11.84 MIMSKK138 pKa = 9.89 CPTMAGPTSTGTYY151 pKa = 7.68 MAAIHH156 pKa = 6.28 EE157 pKa = 4.49 RR158 pKa = 11.84 LSTPSDD164 pKa = 3.29 LRR166 pKa = 11.84 HH167 pKa = 6.52 LFTKK171 pKa = 9.88 MVLYY175 pKa = 10.65 LYY177 pKa = 9.94 DD178 pKa = 3.32 YY179 pKa = 9.99 TLAVVSKK186 pKa = 10.44 DD187 pKa = 3.59 NTMHH191 pKa = 6.37 TADD194 pKa = 5.76 DD195 pKa = 3.47 ISFDD199 pKa = 4.2 LRR201 pKa = 11.84 PHH203 pKa = 6.74 LPSDD207 pKa = 3.56 ALLATVINHH216 pKa = 6.67 DD217 pKa = 3.83 MVIDD221 pKa = 4.23 GDD223 pKa = 4.43 LFTPEE228 pKa = 4.52 QISLLCLAGQQYY240 pKa = 10.23 PSVWYY245 pKa = 10.03 AGEE248 pKa = 3.98 GNIYY252 pKa = 10.31 NSCNMVADD260 pKa = 4.24 DD261 pKa = 4.62 LVVVSSGRR269 pKa = 11.84 LTTDD273 pKa = 3.3 SAFTWGSPDD282 pKa = 3.46 KK283 pKa = 10.03 LYY285 pKa = 11.74 NMMWTIAQKK294 pKa = 11.04 LNGVSCLMYY303 pKa = 10.62 ALEE306 pKa = 4.5 SMRR309 pKa = 11.84 GKK311 pKa = 10.46 CKK313 pKa = 9.91 MMSDD317 pKa = 3.21 IVAKK321 pKa = 9.49 TDD323 pKa = 3.41 CRR325 pKa = 11.84 EE326 pKa = 3.92 VNAMIPRR333 pKa = 11.84 SYY335 pKa = 11.26 CMSTAFGQIRR345 pKa = 11.84 EE346 pKa = 4.11 KK347 pKa = 10.87 QIVVKK352 pKa = 10.0 MPGYY356 pKa = 9.76 FSTSIGMLSDD366 pKa = 3.81 LMYY369 pKa = 11.34 GMTFKK374 pKa = 10.92 AVASCVAEE382 pKa = 4.01 TLGAMGTIVSSSTPRR397 pKa = 11.84 TNPTINGLMRR407 pKa = 11.84 DD408 pKa = 3.64 YY409 pKa = 11.49 GLQHH413 pKa = 5.99 TNAWDD418 pKa = 3.47 NFMLRR423 pKa = 11.84 NFEE426 pKa = 4.2 MVTRR430 pKa = 11.84 RR431 pKa = 11.84 PTQWDD436 pKa = 2.82 IGQHH440 pKa = 4.05 MKK442 pKa = 10.57 EE443 pKa = 4.13 YY444 pKa = 10.66 ALALAEE450 pKa = 4.29 HH451 pKa = 6.36 VMLGYY456 pKa = 10.44 DD457 pKa = 3.06 IEE459 pKa = 4.5 MPSILLTIPALTAVNTAYY477 pKa = 10.73 GLTRR481 pKa = 11.84 GWYY484 pKa = 9.93 GGGSTLDD491 pKa = 3.28 MDD493 pKa = 4.11 KK494 pKa = 10.81 KK495 pKa = 10.25 QRR497 pKa = 11.84 KK498 pKa = 8.33 EE499 pKa = 3.92 STDD502 pKa = 3.38 ALCAVGWMCGLRR514 pKa = 11.84 QCRR517 pKa = 11.84 PQVFRR522 pKa = 11.84 NRR524 pKa = 11.84 AGKK527 pKa = 8.94 KK528 pKa = 7.3 QVMVNAAEE536 pKa = 4.0 RR537 pKa = 11.84 KK538 pKa = 9.23 LRR540 pKa = 11.84 AEE542 pKa = 4.56 AGDD545 pKa = 3.65 DD546 pKa = 3.42 CRR548 pKa = 11.84 IRR550 pKa = 11.84 DD551 pKa = 3.56 VEE553 pKa = 4.57 FWLEE557 pKa = 3.88 DD558 pKa = 3.74 TPGGRR563 pKa = 11.84 VDD565 pKa = 4.04 EE566 pKa = 4.82 NEE568 pKa = 3.72 EE569 pKa = 4.19 SAPNLYY575 pKa = 8.59 KK576 pKa = 10.56 TEE578 pKa = 4.18 FSGTKK583 pKa = 9.44 CAMVFNYY590 pKa = 9.94 EE591 pKa = 3.38 MGMWIEE597 pKa = 4.21 ARR599 pKa = 11.84 QMDD602 pKa = 4.49 YY603 pKa = 11.52 DD604 pKa = 3.7 RR605 pKa = 11.84 LKK607 pKa = 11.0 RR608 pKa = 11.84 EE609 pKa = 4.2 TFSGDD614 pKa = 2.95 LTKK617 pKa = 10.53 KK618 pKa = 9.89 EE619 pKa = 4.38 RR620 pKa = 11.84 YY621 pKa = 8.06 TMSKK625 pKa = 9.43 VSAMPIHH632 pKa = 6.97 WGPPPNHH639 pKa = 6.72 KK640 pKa = 10.61 AKK642 pKa = 10.91 LEE644 pKa = 4.01 ASLEE648 pKa = 4.18 HH649 pKa = 6.36 MKK651 pKa = 10.75 SISRR655 pKa = 11.84 GNAIVPTRR663 pKa = 11.84 EE664 pKa = 4.02 PKK666 pKa = 9.85 HH667 pKa = 6.69 VRR669 pKa = 11.84 INSQSMAVVPKK680 pKa = 9.34 YY681 pKa = 11.0 VKK683 pKa = 10.75 DD684 pKa = 3.55 GVEE687 pKa = 3.83 EE688 pKa = 4.02 EE689 pKa = 4.9 KK690 pKa = 10.75 YY691 pKa = 9.68 VHH693 pKa = 5.89 YY694 pKa = 9.71 EE695 pKa = 3.75 RR696 pKa = 11.84 PAIEE700 pKa = 4.4 EE701 pKa = 4.1 GDD703 pKa = 3.63 TIRR706 pKa = 11.84 FSEE709 pKa = 4.23 IDD711 pKa = 3.48 VPGDD715 pKa = 3.73 GSCGIHH721 pKa = 7.4 AMVKK725 pKa = 10.22 DD726 pKa = 3.73 LTVHH730 pKa = 6.1 GRR732 pKa = 11.84 LSPHH736 pKa = 6.23 EE737 pKa = 3.98 AAKK740 pKa = 9.44 ATEE743 pKa = 4.38 LFSTDD748 pKa = 2.93 TASKK752 pKa = 9.99 KK753 pKa = 10.2 FHH755 pKa = 7.22 DD756 pKa = 4.48 AAEE759 pKa = 4.33 LAAQCQLWGMGMDD772 pKa = 5.87 LIDD775 pKa = 4.05 KK776 pKa = 10.11 GSNRR780 pKa = 11.84 VTRR783 pKa = 11.84 YY784 pKa = 9.86 GPEE787 pKa = 3.82 DD788 pKa = 3.44 SEE790 pKa = 4.49 YY791 pKa = 10.65 SITIIRR797 pKa = 11.84 DD798 pKa = 3.19 GGHH801 pKa = 5.81 FRR803 pKa = 11.84 AGLIGEE809 pKa = 5.04 GANEE813 pKa = 3.8 MTVEE817 pKa = 4.06 HH818 pKa = 7.08 LEE820 pKa = 4.15 QQTRR824 pKa = 11.84 APEE827 pKa = 3.92 EE828 pKa = 3.7 FVRR831 pKa = 11.84 DD832 pKa = 4.06 VKK834 pKa = 11.38 SLGSLFGGSPILQQ847 pKa = 3.42
Molecular weight: 94.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.725
IPC2_protein 5.703
IPC_protein 5.753
Toseland 6.109
ProMoST 6.084
Dawson 5.995
Bjellqvist 6.02
Wikipedia 5.995
Rodwell 5.982
Grimsley 6.249
Solomon 5.995
Lehninger 5.982
Nozaki 6.262
DTASelect 6.44
Thurlkill 6.453
EMBOSS 6.415
Sillero 6.364
Patrickios 4.113
IPC_peptide 6.008
IPC2_peptide 6.351
IPC2.peptide.svr19 6.301
Protein with the highest isoelectric point:
>sp|Q8JVC2|RDRP_PCVC RNA-directed RNA polymerase OS=Penicillium chrysogenum virus (isolate Caston/2003) OX=654932 GN=p1 PE=4 SV=1
MM1 pKa = 7.7 AAPVLYY7 pKa = 10.52 GGAGGTATGPGDD19 pKa = 3.44 MRR21 pKa = 11.84 RR22 pKa = 11.84 SLMHH26 pKa = 6.44 EE27 pKa = 4.25 KK28 pKa = 10.35 KK29 pKa = 10.2 QVFAEE34 pKa = 4.16 LRR36 pKa = 11.84 RR37 pKa = 11.84 EE38 pKa = 4.05 AQALRR43 pKa = 11.84 VAKK46 pKa = 9.6 EE47 pKa = 3.81 ARR49 pKa = 11.84 GKK51 pKa = 9.94 MSVWDD56 pKa = 3.73 PSTRR60 pKa = 11.84 EE61 pKa = 3.49 GARR64 pKa = 11.84 GYY66 pKa = 9.98 RR67 pKa = 11.84 EE68 pKa = 3.61 KK69 pKa = 10.82 VVRR72 pKa = 11.84 FGRR75 pKa = 11.84 QIASLLQYY83 pKa = 9.67 FEE85 pKa = 5.33 NMHH88 pKa = 6.43 SPALDD93 pKa = 4.05 IIACDD98 pKa = 3.61 KK99 pKa = 10.96 FLLKK103 pKa = 10.4 YY104 pKa = 10.25 QIYY107 pKa = 10.69 GDD109 pKa = 3.91 IDD111 pKa = 3.75 RR112 pKa = 11.84 DD113 pKa = 3.56 PAFGEE118 pKa = 3.91 NTMTAEE124 pKa = 4.73 VPVVWDD130 pKa = 3.31 KK131 pKa = 12.04 CEE133 pKa = 4.24 VEE135 pKa = 4.4 VKK137 pKa = 10.38 LYY139 pKa = 10.69 AGPLQKK145 pKa = 10.84 LMSRR149 pKa = 11.84 AKK151 pKa = 10.29 LVGAARR157 pKa = 11.84 EE158 pKa = 4.54 GIPNRR163 pKa = 11.84 NDD165 pKa = 2.98 VAKK168 pKa = 9.95 STGWNQDD175 pKa = 3.23 QVQKK179 pKa = 11.0 FPDD182 pKa = 3.4 NRR184 pKa = 11.84 MDD186 pKa = 3.8 SLISLLEE193 pKa = 3.85 QMQTGQSKK201 pKa = 8.36 LTRR204 pKa = 11.84 LVKK207 pKa = 10.65 GFLILLEE214 pKa = 3.88 MAEE217 pKa = 4.0 RR218 pKa = 11.84 KK219 pKa = 9.51 EE220 pKa = 4.04 VDD222 pKa = 4.05 FHH224 pKa = 7.73 VGNHH228 pKa = 4.56 IHH230 pKa = 5.23 VTYY233 pKa = 10.51 AIAPVCDD240 pKa = 4.3 SYY242 pKa = 12.03 DD243 pKa = 3.62 LPGRR247 pKa = 11.84 CYY249 pKa = 10.96 VFNSKK254 pKa = 8.49 PTSEE258 pKa = 3.76 AHH260 pKa = 6.17 AAVLLAMCRR269 pKa = 11.84 EE270 pKa = 4.1 YY271 pKa = 10.51 PPPQFASHH279 pKa = 5.98 VSVPADD285 pKa = 3.53 AEE287 pKa = 4.39 DD288 pKa = 3.6 VCIVSQGRR296 pKa = 11.84 QIQPGSAVTLNPGLVYY312 pKa = 10.76 SSILTYY318 pKa = 11.49 AMDD321 pKa = 4.19 TSCTDD326 pKa = 4.28 LLQEE330 pKa = 4.33 AQIIACSLQEE340 pKa = 3.53 NRR342 pKa = 11.84 YY343 pKa = 8.48 FSRR346 pKa = 11.84 IGLPTVVSLYY356 pKa = 11.21 DD357 pKa = 3.44 LMVPAFIAQNSALEE371 pKa = 4.11 GARR374 pKa = 11.84 LSGDD378 pKa = 3.11 LSKK381 pKa = 11.24 AVGRR385 pKa = 11.84 VHH387 pKa = 6.94 QMLGMVAAKK396 pKa = 10.23 DD397 pKa = 3.88 IISATHH403 pKa = 4.66 MQSRR407 pKa = 11.84 TGFDD411 pKa = 3.32 PSHH414 pKa = 7.51 GIRR417 pKa = 11.84 QYY419 pKa = 11.54 LNSNSRR425 pKa = 11.84 LVTQMASKK433 pKa = 9.23 LTGIGLFDD441 pKa = 4.12 ATPQMRR447 pKa = 11.84 IFSEE451 pKa = 4.18 MDD453 pKa = 2.86 TADD456 pKa = 3.91 YY457 pKa = 11.52 ADD459 pKa = 4.38 MLHH462 pKa = 5.81 LTIFEE467 pKa = 4.43 GLWLVQDD474 pKa = 4.92 ASVCTDD480 pKa = 3.24 NGPISFLVNGEE491 pKa = 4.28 KK492 pKa = 10.71 LLSADD497 pKa = 3.57 RR498 pKa = 11.84 AGYY501 pKa = 10.0 DD502 pKa = 3.29 VLVEE506 pKa = 4.22 EE507 pKa = 4.84 LTLANIRR514 pKa = 11.84 IEE516 pKa = 4.04 HH517 pKa = 6.44 HH518 pKa = 6.52 KK519 pKa = 10.08 MPTGAFTTRR528 pKa = 11.84 WVAAKK533 pKa = 9.85 RR534 pKa = 11.84 DD535 pKa = 3.59 SALRR539 pKa = 11.84 LTPRR543 pKa = 11.84 SRR545 pKa = 11.84 TAHH548 pKa = 5.67 RR549 pKa = 11.84 VDD551 pKa = 3.56 MVRR554 pKa = 11.84 EE555 pKa = 4.04 CDD557 pKa = 4.52 FNPTMNLKK565 pKa = 10.35 AAGPKK570 pKa = 9.93 ARR572 pKa = 11.84 LRR574 pKa = 11.84 GSGVKK579 pKa = 9.52 SRR581 pKa = 11.84 RR582 pKa = 11.84 RR583 pKa = 11.84 VSEE586 pKa = 3.93 VPLAHH591 pKa = 6.81 VFRR594 pKa = 11.84 SPPRR598 pKa = 11.84 RR599 pKa = 11.84 EE600 pKa = 4.09 STTTTDD606 pKa = 5.2 DD607 pKa = 3.38 SPRR610 pKa = 11.84 WLTRR614 pKa = 11.84 EE615 pKa = 4.27 GPQLTRR621 pKa = 11.84 RR622 pKa = 11.84 VPIIDD627 pKa = 3.98 EE628 pKa = 4.35 PPAYY632 pKa = 9.82 EE633 pKa = 4.27 SGRR636 pKa = 11.84 SSSPVTSSISEE647 pKa = 3.97 GTSQHH652 pKa = 5.41 EE653 pKa = 4.35 EE654 pKa = 3.86 EE655 pKa = 5.02 MGLFDD660 pKa = 5.81 AEE662 pKa = 4.47 EE663 pKa = 4.97 LPMQQTVIATEE674 pKa = 3.56 ARR676 pKa = 11.84 RR677 pKa = 11.84 RR678 pKa = 11.84 LGRR681 pKa = 11.84 GTLEE685 pKa = 4.83 RR686 pKa = 11.84 IQEE689 pKa = 4.15 AALEE693 pKa = 4.28 GQVAQGEE700 pKa = 4.59 VTAEE704 pKa = 3.68 KK705 pKa = 10.46 NRR707 pKa = 11.84 RR708 pKa = 11.84 IEE710 pKa = 4.09 AMLSARR716 pKa = 11.84 DD717 pKa = 3.58 PQFTGRR723 pKa = 11.84 EE724 pKa = 4.2 QITKK728 pKa = 8.74 MLSDD732 pKa = 3.68 GGLGVRR738 pKa = 11.84 EE739 pKa = 3.84 RR740 pKa = 11.84 EE741 pKa = 3.8 EE742 pKa = 3.74 WLEE745 pKa = 3.96 LVDD748 pKa = 3.61 KK749 pKa = 10.3 TVGVKK754 pKa = 9.91 GLKK757 pKa = 8.54 EE758 pKa = 3.81 VRR760 pKa = 11.84 SIDD763 pKa = 3.7 GIRR766 pKa = 11.84 RR767 pKa = 11.84 HH768 pKa = 5.5 LEE770 pKa = 3.71 EE771 pKa = 4.71 YY772 pKa = 10.87 GEE774 pKa = 4.3 RR775 pKa = 11.84 EE776 pKa = 3.81 GFAVVRR782 pKa = 11.84 TLLSGNSKK790 pKa = 9.74 HH791 pKa = 6.25 VRR793 pKa = 11.84 RR794 pKa = 11.84 INQLIRR800 pKa = 11.84 EE801 pKa = 4.45 SNPSAFEE808 pKa = 4.16 TEE810 pKa = 3.93 ASRR813 pKa = 11.84 MRR815 pKa = 11.84 RR816 pKa = 11.84 LRR818 pKa = 11.84 ADD820 pKa = 3.02 WDD822 pKa = 3.69 GDD824 pKa = 3.73 AGSAPVNALHH834 pKa = 6.79 FVGNSPGWKK843 pKa = 9.09 RR844 pKa = 11.84 WLEE847 pKa = 4.04 NNNIPSDD854 pKa = 3.49 IQVAGKK860 pKa = 10.47 KK861 pKa = 9.93 RR862 pKa = 11.84 MCSYY866 pKa = 10.25 LAEE869 pKa = 4.29 VLSHH873 pKa = 6.67 GNLKK877 pKa = 10.79 LSDD880 pKa = 3.43 ATKK883 pKa = 10.54 LGRR886 pKa = 11.84 LVEE889 pKa = 4.32 GTSLDD894 pKa = 3.74 LFPPQLSSEE903 pKa = 4.22 EE904 pKa = 4.17 FSTCSEE910 pKa = 3.94 ATLAWRR916 pKa = 11.84 NAPSSLGVRR925 pKa = 11.84 PFAQEE930 pKa = 3.62 DD931 pKa = 4.09 SRR933 pKa = 11.84 WLVMAATCGGGSFGIGKK950 pKa = 9.71 LKK952 pKa = 10.54 SLCKK956 pKa = 10.02 EE957 pKa = 3.72 FSVPKK962 pKa = 9.77 EE963 pKa = 3.65 LRR965 pKa = 11.84 DD966 pKa = 3.7 ALRR969 pKa = 11.84 VKK971 pKa = 10.86 YY972 pKa = 10.54 GLFGGKK978 pKa = 9.8 DD979 pKa = 3.26 SLEE982 pKa = 4.0
Molecular weight: 108.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.904
IPC2_protein 7.307
IPC_protein 7.176
Toseland 6.971
ProMoST 7.717
Dawson 7.863
Bjellqvist 8.244
Wikipedia 7.775
Rodwell 7.878
Grimsley 7.059
Solomon 7.907
Lehninger 7.922
Nozaki 8.478
DTASelect 8.009
Thurlkill 8.068
EMBOSS 8.097
Sillero 8.375
Patrickios 4.673
IPC_peptide 7.907
IPC2_peptide 7.351
IPC2.peptide.svr19 7.267
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3858
847
1117
964.5
108.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.491 ± 0.493
1.788 ± 0.122
5.677 ± 0.143
7.284 ± 0.22
3.37 ± 0.594
7.517 ± 0.438
2.54 ± 0.15
4.303 ± 0.152
5.21 ± 0.252
9.253 ± 0.505
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.733 ± 0.508
3.344 ± 0.338
4.044 ± 0.331
3.24 ± 0.236
7.102 ± 0.408
6.713 ± 0.418
5.469 ± 0.337
7.154 ± 0.47
1.788 ± 0.218
2.981 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here