Hubei picorna-like virus 74
Average proteome isoelectric point is 5.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLY4|A0A1L3KLY4_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 74 OX=1923158 PE=4 SV=1
MM1 pKa = 7.67 AGFAAAGAAALGIGASFASPFINQSLDD28 pKa = 3.23 YY29 pKa = 11.27 QNFLKK34 pKa = 9.6 QQNYY38 pKa = 6.76 MQSQYY43 pKa = 10.8 EE44 pKa = 4.51 KK45 pKa = 10.73 YY46 pKa = 10.16 GLPYY50 pKa = 10.8 LPGFDD55 pKa = 4.66 FKK57 pKa = 11.12 NAPPSSFVNHH67 pKa = 6.42 SSGTNIEE74 pKa = 4.11 ISHH77 pKa = 6.08 MPFMIGGAFGTMFGTNDD94 pKa = 3.13 WGNQGSSGDD103 pKa = 3.92 GVNSSRR109 pKa = 11.84 DD110 pKa = 3.33 SGSAYY115 pKa = 9.76 PGLGYY120 pKa = 10.72 GNNYY124 pKa = 9.51 SAVPPPPSYY133 pKa = 11.15 SSGGQRR139 pKa = 11.84 DD140 pKa = 4.01 LVQGKK145 pKa = 8.69 PGLSGGIQYY154 pKa = 11.12 SLNTLNTPDD163 pKa = 3.43 EE164 pKa = 4.39 SEE166 pKa = 4.14 YY167 pKa = 11.18 AGPINTRR174 pKa = 11.84 SVYY177 pKa = 9.84 TSALVRR183 pKa = 11.84 PPALNFSHH191 pKa = 6.71 EE192 pKa = 4.28 VADD195 pKa = 4.11 LSGASGVHH203 pKa = 4.9 RR204 pKa = 11.84 TTLNTQYY211 pKa = 10.46 FSSTRR216 pKa = 11.84 PVPTTFSVQADD227 pKa = 3.7 VHH229 pKa = 6.14 TPIQLNSGVKK239 pKa = 9.72 PDD241 pKa = 4.14 FGQNLGLPSRR251 pKa = 11.84 PFSSVSRR258 pKa = 11.84 VTRR261 pKa = 11.84 GSSVATDD268 pKa = 3.34 SDD270 pKa = 4.64 EE271 pKa = 4.51 ILPDD275 pKa = 4.47 PDD277 pKa = 3.83 TTDD280 pKa = 3.37 SANLSSDD287 pKa = 3.94 FQSSTADD294 pKa = 3.17 SEE296 pKa = 4.66 ANNNIVSEE304 pKa = 4.26 DD305 pKa = 3.72 APIGAVDD312 pKa = 4.57 DD313 pKa = 4.35 SADD316 pKa = 3.37 IDD318 pKa = 3.85 AEE320 pKa = 4.51 SILGSSAGIPLL331 pKa = 3.79
Molecular weight: 34.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.135
IPC2_protein 4.24
IPC_protein 4.228
Toseland 4.024
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.368
Wikipedia 4.164
Rodwell 4.062
Grimsley 3.935
Solomon 4.215
Lehninger 4.177
Nozaki 4.329
DTASelect 4.596
Thurlkill 4.062
EMBOSS 4.177
Sillero 4.355
Patrickios 3.032
IPC_peptide 4.215
IPC2_peptide 4.329
IPC2.peptide.svr19 4.261
Protein with the highest isoelectric point:
>tr|A0A1L3KLX7|A0A1L3KLX7_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 74 OX=1923158 PE=4 SV=1
MM1 pKa = 7.27 SCSKK5 pKa = 10.42 RR6 pKa = 11.84 ALIDD10 pKa = 3.45 CDD12 pKa = 3.93 VVKK15 pKa = 10.91 DD16 pKa = 3.98 CIVPNVEE23 pKa = 4.43 SRR25 pKa = 11.84 EE26 pKa = 3.92 TDD28 pKa = 2.97 LSSFYY33 pKa = 9.56 QLCFIEE39 pKa = 4.64 PNKK42 pKa = 10.4 QSTDD46 pKa = 3.37 VEE48 pKa = 4.73 DD49 pKa = 3.86 NSNLEE54 pKa = 3.99 EE55 pKa = 4.31 DD56 pKa = 4.26 LKK58 pKa = 11.42 SVSQCYY64 pKa = 10.0 NYY66 pKa = 10.6 SDD68 pKa = 4.13 SEE70 pKa = 4.29 EE71 pKa = 5.1 GDD73 pKa = 3.65 VLSSVLSLNSAVFSVLSRR91 pKa = 11.84 AKK93 pKa = 10.38 LLNLIDD99 pKa = 3.56 QLSNVFVADD108 pKa = 3.83 SFVSALAPLARR119 pKa = 11.84 ICEE122 pKa = 4.35 LYY124 pKa = 10.65 GYY126 pKa = 9.97 LWSDD130 pKa = 3.46 VNGQMGVLEE139 pKa = 4.38 ILWNVLVWMKK149 pKa = 11.04 DD150 pKa = 3.34 LIVPLFKK157 pKa = 10.61 GSKK160 pKa = 9.47 LVPDD164 pKa = 4.29 DD165 pKa = 3.86 VKK167 pKa = 11.43 SRR169 pKa = 11.84 LPDD172 pKa = 3.54 TEE174 pKa = 4.03 PVKK177 pKa = 10.61 QAFVKK182 pKa = 10.67 LSEE185 pKa = 4.19 DD186 pKa = 3.08 RR187 pKa = 11.84 KK188 pKa = 10.81 GKK190 pKa = 10.08 IKK192 pKa = 10.95 SFFEE196 pKa = 4.42 NIGCHH201 pKa = 4.91 EE202 pKa = 4.23 LAGRR206 pKa = 11.84 VPYY209 pKa = 10.21 LVPVLATVVTGALAFVAFRR228 pKa = 11.84 FGPAMLQQKK237 pKa = 9.14 EE238 pKa = 4.26 MNFVKK243 pKa = 10.49 SVTAMGMLARR253 pKa = 11.84 SSHH256 pKa = 5.5 FLRR259 pKa = 11.84 AEE261 pKa = 3.92 FASIWKK267 pKa = 9.73 VATDD271 pKa = 3.87 CFAEE275 pKa = 4.18 IFGLAWVEE283 pKa = 3.93 DD284 pKa = 3.65 ADD286 pKa = 4.62 IEE288 pKa = 4.58 VKK290 pKa = 10.51 KK291 pKa = 10.83 YY292 pKa = 10.72 ISDD295 pKa = 3.52 LHH297 pKa = 7.68 SLYY300 pKa = 10.89 KK301 pKa = 10.43 EE302 pKa = 3.65 FRR304 pKa = 11.84 QYY306 pKa = 10.31 QNRR309 pKa = 11.84 ILSDD313 pKa = 3.69 PYY315 pKa = 11.42 VVFGDD320 pKa = 3.79 QEE322 pKa = 4.11 FLPRR326 pKa = 11.84 LEE328 pKa = 4.55 LQLKK332 pKa = 10.24 RR333 pKa = 11.84 LDD335 pKa = 3.88 DD336 pKa = 4.22 FYY338 pKa = 11.88 LRR340 pKa = 11.84 LASAKK345 pKa = 10.56 KK346 pKa = 10.5 NMVNAKK352 pKa = 9.9 SALDD356 pKa = 3.59 SLRR359 pKa = 11.84 EE360 pKa = 3.79 MAKK363 pKa = 10.16 EE364 pKa = 3.77 SRR366 pKa = 11.84 LLVDD370 pKa = 4.23 NIKK373 pKa = 9.34 RR374 pKa = 11.84 TCTEE378 pKa = 3.77 KK379 pKa = 10.51 IEE381 pKa = 4.18 PVVIWLSGAAGIGKK395 pKa = 9.18 SRR397 pKa = 11.84 AVSKK401 pKa = 10.58 IVEE404 pKa = 4.35 EE405 pKa = 4.08 LGKK408 pKa = 10.49 LHH410 pKa = 7.13 GEE412 pKa = 4.11 VLHH415 pKa = 6.25 TYY417 pKa = 10.07 VRR419 pKa = 11.84 GTADD423 pKa = 3.78 QYY425 pKa = 9.4 WSGYY429 pKa = 8.9 SGQPVVIYY437 pKa = 10.72 DD438 pKa = 3.53 DD439 pKa = 4.15 FGNSKK444 pKa = 10.34 EE445 pKa = 4.08 KK446 pKa = 10.61 TEE448 pKa = 4.02 FTEE451 pKa = 4.11 LVSIKK456 pKa = 9.4 TCQAFLLNCAAVEE469 pKa = 4.16 EE470 pKa = 4.44 KK471 pKa = 11.11 GKK473 pKa = 10.58 LFTSKK478 pKa = 10.94 YY479 pKa = 10.81 VIICANMLDD488 pKa = 4.14 VVNTAQIPNVEE499 pKa = 4.14 VLNRR503 pKa = 11.84 RR504 pKa = 11.84 RR505 pKa = 11.84 DD506 pKa = 3.54 FLFVVEE512 pKa = 5.05 NPEE515 pKa = 3.78 LTNYY519 pKa = 8.91 VQVNGRR525 pKa = 11.84 EE526 pKa = 3.99 PTADD530 pKa = 3.48 CDD532 pKa = 2.95 IWKK535 pKa = 10.23 DD536 pKa = 3.85 DD537 pKa = 4.1 FSHH540 pKa = 5.42 LTIRR544 pKa = 11.84 RR545 pKa = 11.84 RR546 pKa = 11.84 AALPRR551 pKa = 11.84 DD552 pKa = 3.43 RR553 pKa = 11.84 DD554 pKa = 3.6 AVRR557 pKa = 11.84 DD558 pKa = 3.73 NSEE561 pKa = 3.48 WDD563 pKa = 3.48 VFRR566 pKa = 11.84 IAQEE570 pKa = 3.92 SYY572 pKa = 9.35 VRR574 pKa = 11.84 FRR576 pKa = 11.84 TAEE579 pKa = 3.75 QKK581 pKa = 10.35 FYY583 pKa = 11.01 HH584 pKa = 6.41 YY585 pKa = 10.3 LTDD588 pKa = 3.58 LQTRR592 pKa = 11.84 RR593 pKa = 11.84 QRR595 pKa = 11.84 LQDD598 pKa = 3.42 AVEE601 pKa = 4.86 DD602 pKa = 4.1 KK603 pKa = 11.13 DD604 pKa = 4.13 GLPSAPVCAVRR615 pKa = 11.84 GGGEE619 pKa = 4.14 SSVSDD624 pKa = 3.72 SEE626 pKa = 4.16 VLEE629 pKa = 4.09 RR630 pKa = 11.84 FGFLFDD636 pKa = 3.36 TDD638 pKa = 3.38 EE639 pKa = 4.54 PEE641 pKa = 3.82 RR642 pKa = 11.84 QFFHH646 pKa = 6.68 QFEE649 pKa = 4.53 RR650 pKa = 11.84 QIHH653 pKa = 4.7 MPILFVGEE661 pKa = 4.13 AGTGKK666 pKa = 8.2 TFTMHH671 pKa = 7.28 AIRR674 pKa = 11.84 RR675 pKa = 11.84 RR676 pKa = 11.84 FEE678 pKa = 3.74 QEE680 pKa = 3.49 KK681 pKa = 10.43 LKK683 pKa = 11.03 VRR685 pKa = 11.84 MFSGTQFQTFFDD697 pKa = 4.07 GGGKK701 pKa = 6.24 VTKK704 pKa = 10.3 YY705 pKa = 10.84 DD706 pKa = 3.57 VILIEE711 pKa = 4.93 DD712 pKa = 4.03 FTVTAEE718 pKa = 3.8 AMNAFRR724 pKa = 11.84 TFVNEE729 pKa = 3.97 AEE731 pKa = 4.74 DD732 pKa = 3.96 GFFGKK737 pKa = 10.34 KK738 pKa = 9.05 IVVATGNLSLVHH750 pKa = 6.31 DD751 pKa = 4.71 HH752 pKa = 6.75 AAGRR756 pKa = 11.84 GVDD759 pKa = 3.13 WLAAFEE765 pKa = 4.16 RR766 pKa = 11.84 RR767 pKa = 11.84 VRR769 pKa = 11.84 IYY771 pKa = 10.13 QFSFKK776 pKa = 10.45 RR777 pKa = 11.84 RR778 pKa = 11.84 WLFKK782 pKa = 8.65 YY783 pKa = 9.11 TPADD787 pKa = 3.39 VVANPEE793 pKa = 4.09 NYY795 pKa = 10.39 DD796 pKa = 3.3 VMVRR800 pKa = 11.84 ISASTLTTQEE810 pKa = 4.12 RR811 pKa = 11.84 SDD813 pKa = 3.61 LFQRR817 pKa = 11.84 DD818 pKa = 3.71 LIDD821 pKa = 3.81 FVVQATPRR829 pKa = 11.84 VALYY833 pKa = 8.09 QTFEE837 pKa = 4.22 SLPKK841 pKa = 9.79 IGEE844 pKa = 4.09 VEE846 pKa = 3.97 ATFEE850 pKa = 4.39 VVLKK854 pKa = 7.52 VTPSDD859 pKa = 3.33 FLKK862 pKa = 10.44 WMKK865 pKa = 10.24 SKK867 pKa = 10.74 KK868 pKa = 10.08 GVYY871 pKa = 8.8 TPAGVAAAIAEE882 pKa = 4.35 VHH884 pKa = 6.41 SMISVPFSKK893 pKa = 10.08 MVFFGRR899 pKa = 11.84 FLHH902 pKa = 6.98 SMAAKK907 pKa = 10.62 GIGLSLMSGWRR918 pKa = 11.84 TEE920 pKa = 4.3 RR921 pKa = 11.84 NMLTLRR927 pKa = 11.84 DD928 pKa = 3.57 ILVNVANLNIEE939 pKa = 4.24 FPTSEE944 pKa = 4.18 VVRR947 pKa = 11.84 FRR949 pKa = 11.84 YY950 pKa = 9.89 ADD952 pKa = 3.5 EE953 pKa = 5.76 AYY955 pKa = 10.45 LLCASDD961 pKa = 3.81 EE962 pKa = 4.13 GRR964 pKa = 11.84 LCILEE969 pKa = 4.23 EE970 pKa = 4.52 CPDD973 pKa = 3.44 GFVRR977 pKa = 11.84 QTGSFVGGVDD987 pKa = 3.31 YY988 pKa = 10.65 TKK990 pKa = 10.77 DD991 pKa = 3.22 EE992 pKa = 4.45 KK993 pKa = 11.41 EE994 pKa = 3.76 FMQRR998 pKa = 11.84 VSHH1001 pKa = 6.24 VIQRR1005 pKa = 11.84 QRR1007 pKa = 11.84 VAMKK1011 pKa = 9.97 EE1012 pKa = 4.03 KK1013 pKa = 10.14 QDD1015 pKa = 3.44 KK1016 pKa = 10.14 CGKK1019 pKa = 9.6 AFEE1022 pKa = 4.27 RR1023 pKa = 11.84 MIVSGGLKK1031 pKa = 8.94 TLPAWARR1038 pKa = 11.84 ALFEE1042 pKa = 4.31 AVNVLLLYY1050 pKa = 8.69 GTAVVTLQDD1059 pKa = 2.96 SYY1061 pKa = 11.74 VRR1063 pKa = 11.84 YY1064 pKa = 9.57 RR1065 pKa = 11.84 QLQDD1069 pKa = 3.43 LKK1071 pKa = 10.93 EE1072 pKa = 4.23 KK1073 pKa = 10.98 KK1074 pKa = 10.01 NLLDD1078 pKa = 3.78 EE1079 pKa = 4.91 FCTPEE1084 pKa = 5.13 LEE1086 pKa = 4.29 GQKK1089 pKa = 9.77 PSKK1092 pKa = 11.11 VEE1094 pKa = 3.54 ITEE1097 pKa = 4.21 TFVLKK1102 pKa = 10.56 DD1103 pKa = 3.95 PKK1105 pKa = 11.25 VKK1107 pKa = 10.36 GQPEE1111 pKa = 4.09 KK1112 pKa = 10.73 VSTVVHH1118 pKa = 5.84 VNGKK1122 pKa = 7.95 SYY1124 pKa = 11.12 RR1125 pKa = 11.84 EE1126 pKa = 3.86 AGMASWGSDD1135 pKa = 2.62 SSLSSNVSLPPMMRR1149 pKa = 11.84 APKK1152 pKa = 8.4 PTARR1156 pKa = 11.84 RR1157 pKa = 11.84 SAGGGGGTSVTLQDD1171 pKa = 3.46 TPTYY1175 pKa = 8.4 ATKK1178 pKa = 10.4 SYY1180 pKa = 10.93 SYY1182 pKa = 10.18 MRR1184 pKa = 11.84 NLEE1187 pKa = 3.92 VAKK1190 pKa = 10.35 PGLRR1194 pKa = 11.84 LRR1196 pKa = 11.84 EE1197 pKa = 3.89 NSVAPVTTVASSAQPIMAARR1217 pKa = 11.84 YY1218 pKa = 9.18 ADD1220 pKa = 3.63 VVQPSQYY1227 pKa = 11.26 DD1228 pKa = 3.52 LDD1230 pKa = 5.78 RR1231 pKa = 11.84 IANMQRR1237 pKa = 11.84 VYY1239 pKa = 10.85 EE1240 pKa = 4.19 KK1241 pKa = 10.94 NGLQRR1246 pKa = 11.84 TVLMPSPTLQKK1257 pKa = 10.58 KK1258 pKa = 10.4 LEE1260 pKa = 4.46 TIVEE1264 pKa = 4.2 SEE1266 pKa = 4.28 EE1267 pKa = 3.9 ISNEE1271 pKa = 3.71 VLDD1274 pKa = 4.29 QLNCEE1279 pKa = 4.09 EE1280 pKa = 5.15 FEE1282 pKa = 4.43 VQRR1285 pKa = 11.84 EE1286 pKa = 4.35 SQSDD1290 pKa = 3.81 PQAHH1294 pKa = 7.09 DD1295 pKa = 3.56 LAEE1298 pKa = 4.14 LCIVNSVSIVYY1309 pKa = 10.35 ADD1311 pKa = 3.74 SKK1313 pKa = 8.15 EE1314 pKa = 4.02 HH1315 pKa = 6.02 CVYY1318 pKa = 11.28 GLMIAEE1324 pKa = 4.23 DD1325 pKa = 3.55 VGVTVLHH1332 pKa = 6.67 CASACLNVEE1341 pKa = 4.05 ILSTPGVYY1349 pKa = 10.34 YY1350 pKa = 11.11 DD1351 pKa = 4.04 MVLLSKK1357 pKa = 10.9 DD1358 pKa = 3.06 RR1359 pKa = 11.84 TRR1361 pKa = 11.84 DD1362 pKa = 3.31 LMIFRR1367 pKa = 11.84 VQSCPAKK1374 pKa = 10.1 FRR1376 pKa = 11.84 NITNHH1381 pKa = 5.92 LRR1383 pKa = 11.84 SEE1385 pKa = 4.69 LYY1387 pKa = 7.56 TTEE1390 pKa = 4.14 IDD1392 pKa = 3.31 GHH1394 pKa = 6.5 DD1395 pKa = 3.93 AMLVTPSSRR1404 pKa = 11.84 NRR1406 pKa = 11.84 VGWKK1410 pKa = 8.33 RR1411 pKa = 11.84 WAVVRR1416 pKa = 11.84 ACVSRR1421 pKa = 11.84 KK1422 pKa = 9.08 YY1423 pKa = 10.54 AGSDD1427 pKa = 3.42 VVEE1430 pKa = 4.43 HH1431 pKa = 6.24 EE1432 pKa = 4.05 RR1433 pKa = 11.84 TYY1435 pKa = 11.44 VGMLAGYY1442 pKa = 9.81 KK1443 pKa = 9.67 IVTGVGTASGDD1454 pKa = 3.86 CGSSLILNSARR1465 pKa = 11.84 LPFKK1469 pKa = 10.93 LIGIHH1474 pKa = 6.91 KK1475 pKa = 10.11 AATDD1479 pKa = 3.66 KK1480 pKa = 11.19 VGYY1483 pKa = 9.26 AAEE1486 pKa = 4.14 IFRR1489 pKa = 11.84 EE1490 pKa = 4.18 DD1491 pKa = 3.2 FQEE1494 pKa = 3.93 FVPIKK1499 pKa = 10.0 EE1500 pKa = 4.43 PNRR1503 pKa = 11.84 QRR1505 pKa = 11.84 ALKK1508 pKa = 9.89 RR1509 pKa = 11.84 DD1510 pKa = 3.87 EE1511 pKa = 4.79 IEE1513 pKa = 4.73 LFQEE1517 pKa = 4.07 PMPVGRR1523 pKa = 11.84 TTQIVGRR1530 pKa = 11.84 AVFRR1534 pKa = 11.84 NHH1536 pKa = 6.14 QSVKK1540 pKa = 7.84 TQFYY1544 pKa = 9.49 RR1545 pKa = 11.84 CPLACPFLDD1554 pKa = 5.35 DD1555 pKa = 3.89 EE1556 pKa = 4.94 FEE1558 pKa = 4.2 PVILSHH1564 pKa = 6.89 QDD1566 pKa = 3.14 FRR1568 pKa = 11.84 CRR1570 pKa = 11.84 DD1571 pKa = 3.06 TDD1573 pKa = 4.49 FYY1575 pKa = 11.15 PEE1577 pKa = 4.0 DD1578 pKa = 5.12 AFLKK1582 pKa = 9.89 WDD1584 pKa = 3.91 GPTGFEE1590 pKa = 4.03 IDD1592 pKa = 4.32 CAPFSADD1599 pKa = 2.4 VRR1601 pKa = 11.84 AQIFEE1606 pKa = 4.28 MVSDD1610 pKa = 4.14 QICDD1614 pKa = 2.88 KK1615 pKa = 10.68 WIFEE1619 pKa = 4.53 CEE1621 pKa = 3.81 RR1622 pKa = 11.84 LHH1624 pKa = 7.5 RR1625 pKa = 11.84 PLFKK1629 pKa = 10.05 LTKK1632 pKa = 9.12 TEE1634 pKa = 4.63 AINRR1638 pKa = 11.84 PSQCIGLSNSLNRR1651 pKa = 11.84 ASSPGYY1657 pKa = 8.95 PFRR1660 pKa = 11.84 HH1661 pKa = 6.01 FGARR1665 pKa = 11.84 RR1666 pKa = 11.84 GKK1668 pKa = 10.62 ADD1670 pKa = 3.5 FLEE1673 pKa = 4.53 FDD1675 pKa = 4.04 EE1676 pKa = 5.72 GQQIWMLAKK1685 pKa = 10.38 NEE1687 pKa = 4.1 HH1688 pKa = 5.83 GRR1690 pKa = 11.84 LLNHH1694 pKa = 6.98 SIDD1697 pKa = 3.9 LFVDD1701 pKa = 4.13 EE1702 pKa = 4.96 ARR1704 pKa = 11.84 RR1705 pKa = 11.84 GNVPPIVYY1713 pKa = 9.48 VANLKK1718 pKa = 10.94 DD1719 pKa = 3.45 EE1720 pKa = 4.28 TRR1722 pKa = 11.84 KK1723 pKa = 9.91 KK1724 pKa = 10.77 DD1725 pKa = 3.95 RR1726 pKa = 11.84 IYY1728 pKa = 10.26 EE1729 pKa = 4.06 QPKK1732 pKa = 8.41 TRR1734 pKa = 11.84 GFAAAPVHH1742 pKa = 5.44 YY1743 pKa = 10.12 VIAGRR1748 pKa = 11.84 MYY1750 pKa = 10.67 NYY1752 pKa = 10.95 AAMALCAEE1760 pKa = 4.36 ARR1762 pKa = 11.84 AQLGHH1767 pKa = 6.13 VLGMDD1772 pKa = 3.69 PNSLEE1777 pKa = 4.05 WHH1779 pKa = 6.13 SMTLRR1784 pKa = 11.84 LVSKK1788 pKa = 9.59 SNCIINGDD1796 pKa = 3.87 YY1797 pKa = 10.99 KK1798 pKa = 11.32 DD1799 pKa = 3.75 FDD1801 pKa = 3.77 SGHH1804 pKa = 5.6 FRR1806 pKa = 11.84 EE1807 pKa = 5.05 ALALPTRR1814 pKa = 11.84 IRR1816 pKa = 11.84 CEE1818 pKa = 3.58 LYY1820 pKa = 10.39 RR1821 pKa = 11.84 RR1822 pKa = 11.84 LDD1824 pKa = 3.8 PGWRR1828 pKa = 11.84 PEE1830 pKa = 3.8 DD1831 pKa = 3.48 TIIRR1835 pKa = 11.84 EE1836 pKa = 4.32 NIDD1839 pKa = 3.1 RR1840 pKa = 11.84 ASQYY1844 pKa = 11.28 GLLLRR1849 pKa = 11.84 GHH1851 pKa = 7.55 LLFQHH1856 pKa = 6.34 SKK1858 pKa = 11.24 GIFSGQFDD1866 pKa = 3.97 TTFNNCVLNQLYY1878 pKa = 9.56 IVYY1881 pKa = 9.77 AWFVTMFEE1889 pKa = 4.54 KK1890 pKa = 10.72 KK1891 pKa = 10.3 RR1892 pKa = 11.84 FDD1894 pKa = 3.71 LMSLGGFDD1902 pKa = 4.25 KK1903 pKa = 10.24 MCHH1906 pKa = 6.39 LEE1908 pKa = 4.04 VCGDD1912 pKa = 4.03 DD1913 pKa = 4.0 FNLSISDD1920 pKa = 3.93 LDD1922 pKa = 4.14 LFGLQDD1928 pKa = 3.36 IVRR1931 pKa = 11.84 VLSQHH1936 pKa = 5.94 FGIVVTSASAKK1947 pKa = 10.63 DD1948 pKa = 3.59 STQSVLPYY1956 pKa = 10.2 EE1957 pKa = 5.22 DD1958 pKa = 3.62 IYY1960 pKa = 11.49 EE1961 pKa = 4.01 VDD1963 pKa = 3.8 FLKK1966 pKa = 10.6 RR1967 pKa = 11.84 QYY1969 pKa = 11.1 VCIGGRR1975 pKa = 11.84 WCGRR1979 pKa = 11.84 VKK1981 pKa = 10.46 MSVFDD1986 pKa = 5.97 KK1987 pKa = 10.56 MLNWCVGPKK1996 pKa = 9.87 RR1997 pKa = 11.84 CLTPEE2002 pKa = 3.71 NLYY2005 pKa = 11.18 DD2006 pKa = 4.11 EE2007 pKa = 5.2 FFDD2010 pKa = 3.85 MTNMCALMDD2019 pKa = 3.77 VALIEE2024 pKa = 4.05 AVFWGRR2030 pKa = 11.84 EE2031 pKa = 3.97 LYY2033 pKa = 9.97 EE2034 pKa = 4.01 KK2035 pKa = 10.67 LVAHH2039 pKa = 6.64 FRR2041 pKa = 11.84 SFLCQPGIDD2050 pKa = 3.7 IQKK2053 pKa = 10.22 DD2054 pKa = 3.68 LPSYY2058 pKa = 12.02 DD2059 pKa = 3.35 MMCILFEE2066 pKa = 4.39 LVPSDD2071 pKa = 3.13 MRR2073 pKa = 11.84 VFGNVCRR2080 pKa = 11.84 NQIAVTDD2087 pKa = 3.91 KK2088 pKa = 11.35 QMATVKK2094 pKa = 10.09 NAKK2097 pKa = 9.47 PSAAGVEE2104 pKa = 4.13 PQNINVPDD2112 pKa = 4.01 VPVSPPDD2119 pKa = 3.26 QMSPYY2124 pKa = 10.86 APTATNATQQASQGIGCVPLSVPHH2148 pKa = 6.7 IVGKK2152 pKa = 8.3 YY2153 pKa = 6.67 VWNEE2157 pKa = 4.0 TISITTNDD2165 pKa = 3.63 EE2166 pKa = 4.01 PGKK2169 pKa = 8.86 VLWWRR2174 pKa = 11.84 ALHH2177 pKa = 6.72 PSTNPYY2183 pKa = 10.75 ADD2185 pKa = 3.54 WLTKK2189 pKa = 10.21 PFNAWAGGMLFRR2201 pKa = 11.84 ATICANFLCAGKK2213 pKa = 10.33 VVLVHH2218 pKa = 6.53 WMPNQNPTLEE2228 pKa = 4.22 RR2229 pKa = 11.84 TLEE2232 pKa = 4.0 EE2233 pKa = 4.73 VTCQPHH2239 pKa = 5.75 MMCDD2243 pKa = 3.91 LKK2245 pKa = 11.64 NMEE2248 pKa = 4.3 TTEE2251 pKa = 4.61 LLVEE2255 pKa = 4.79 DD2256 pKa = 4.44 EE2257 pKa = 5.81 NPTKK2261 pKa = 8.91 WHH2263 pKa = 6.81 YY2264 pKa = 7.72 YY2265 pKa = 9.84 QPMPQMNTDD2274 pKa = 3.45 PDD2276 pKa = 4.28 GNITPSEE2283 pKa = 4.2 WFSSLGVGGYY2293 pKa = 9.64 IAMYY2297 pKa = 10.0 VYY2299 pKa = 11.0 GRR2301 pKa = 11.84 FNMGEE2306 pKa = 3.99 GSLNSLDD2313 pKa = 4.35 IIVEE2317 pKa = 4.29 SAVAPSFTVNYY2328 pKa = 8.72 LKK2330 pKa = 10.41 SVRR2333 pKa = 11.84 SSDD2336 pKa = 3.55 TPKK2339 pKa = 10.64 PGVAQEE2345 pKa = 3.69 AWQTLKK2351 pKa = 10.92 DD2352 pKa = 3.75 ASIPFCPTSPHH2363 pKa = 6.2 PNKK2366 pKa = 10.32 IIVTPQPVFGFDD2378 pKa = 2.82 TVAQMTCGGTFWDD2391 pKa = 4.27 TNWWKK2396 pKa = 11.28 GDD2398 pKa = 3.52 FEE2400 pKa = 4.45 LAADD2404 pKa = 4.2 RR2405 pKa = 11.84 ATNVGQVNLIPTGTTQSGPTFTATYY2430 pKa = 8.29 DD2431 pKa = 3.4 TFVNEE2436 pKa = 4.1 FVPNKK2441 pKa = 9.45 ISRR2444 pKa = 11.84 VLYY2447 pKa = 9.5 CIRR2450 pKa = 11.84 GGKK2453 pKa = 9.65 FYY2455 pKa = 10.86 DD2456 pKa = 3.55 WTVGSYY2462 pKa = 9.87 FNWNIEE2468 pKa = 3.89 TRR2470 pKa = 11.84 KK2471 pKa = 10.26 YY2472 pKa = 10.39 EE2473 pKa = 4.18 GAKK2476 pKa = 7.83 MTYY2479 pKa = 8.37 TMEE2482 pKa = 4.99 DD2483 pKa = 2.95 AGLPASDD2490 pKa = 4.68 NRR2492 pKa = 11.84 PHH2494 pKa = 7.73 LIGTYY2499 pKa = 10.95 SMDD2502 pKa = 5.39 DD2503 pKa = 3.37 EE2504 pKa = 4.99 FEE2506 pKa = 4.28 YY2507 pKa = 10.01 PAYY2510 pKa = 10.0 PEE2512 pKa = 4.07 DD2513 pKa = 3.55 TKK2515 pKa = 11.41 KK2516 pKa = 10.48 FVPGVPEE2523 pKa = 3.85 SLIMFGRR2530 pKa = 11.84 YY2531 pKa = 8.19 DD2532 pKa = 3.31 QNYY2535 pKa = 7.71 PFPSASFQTQEE2546 pKa = 3.98 MVKK2549 pKa = 10.49 AFYY2552 pKa = 11.11 SKK2554 pKa = 9.34 TSKK2557 pKa = 10.09 IASQITPTTAGLFMIYY2573 pKa = 8.74 DD2574 pKa = 4.24 TKK2576 pKa = 11.35 NRR2578 pKa = 11.84 LPVRR2582 pKa = 11.84 QCKK2585 pKa = 10.01 LYY2587 pKa = 10.6 YY2588 pKa = 10.29 EE2589 pKa = 4.81 GFLTVMASDD2598 pKa = 4.42 KK2599 pKa = 11.17 YY2600 pKa = 11.18 EE2601 pKa = 4.42 IMDD2604 pKa = 4.18 SDD2606 pKa = 4.43 YY2607 pKa = 10.82 EE2608 pKa = 4.18 LQFMSIVDD2616 pKa = 3.81 RR2617 pKa = 11.84 NYY2619 pKa = 10.63 VIKK2622 pKa = 10.67 DD2623 pKa = 3.33 KK2624 pKa = 11.51 QNLIEE2629 pKa = 4.22 NSEE2632 pKa = 4.03 FAKK2635 pKa = 10.78 NQDD2638 pKa = 4.03 RR2639 pKa = 11.84 IRR2641 pKa = 11.84 KK2642 pKa = 7.46 ATAFLEE2648 pKa = 4.37 RR2649 pKa = 11.84 TDD2651 pKa = 3.61 LTKK2654 pKa = 10.74 LEE2656 pKa = 4.18 RR2657 pKa = 11.84 LAGIVTQHH2665 pKa = 6.21 WGSLTRR2671 pKa = 11.84 RR2672 pKa = 3.64
Molecular weight: 302.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.633
IPC2_protein 5.664
IPC_protein 5.703
Toseland 5.944
ProMoST 6.008
Dawson 5.906
Bjellqvist 5.944
Wikipedia 5.881
Rodwell 5.881
Grimsley 6.033
Solomon 5.906
Lehninger 5.893
Nozaki 6.148
DTASelect 6.326
Thurlkill 6.287
EMBOSS 6.249
Sillero 6.237
Patrickios 4.126
IPC_peptide 5.919
IPC2_peptide 6.224
IPC2.peptide.svr19 6.224
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3450
331
2672
1150.0
129.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.928 ± 0.431
1.913 ± 0.503
6.232 ± 0.116
5.739 ± 1.132
5.565 ± 0.311
5.971 ± 1.117
1.681 ± 0.068
4.725 ± 0.293
4.87 ± 1.038
8.319 ± 0.487
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.551 ± 0.43
4.377 ± 0.697
4.986 ± 0.765
4.058 ± 0.213
5.623 ± 0.786
7.42 ± 1.907
5.536 ± 0.105
8.087 ± 0.772
1.362 ± 0.324
4.058 ± 0.974
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here