Shahe narna-like virus 7
Average proteome isoelectric point is 5.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIK7|A0A1L3KIK7_9VIRU RNA-dependent RNA polymerase OS=Shahe narna-like virus 7 OX=1923435 PE=4 SV=1
MM1 pKa = 7.68 PSWLFTSHH9 pKa = 5.88 EE10 pKa = 4.32 SVRR13 pKa = 11.84 IGMTVLTISSGFNLPVSVNLDD34 pKa = 3.81 PIMTPSKK41 pKa = 10.39 SIPSSMEE48 pKa = 3.11 SWEE51 pKa = 3.82 IDD53 pKa = 3.63 LVCRR57 pKa = 11.84 FLGIKK62 pKa = 9.68 EE63 pKa = 4.62 EE64 pKa = 4.14 YY65 pKa = 10.36 VEE67 pKa = 3.91 WFMFHH72 pKa = 7.14 HH73 pKa = 5.95 STKK76 pKa = 10.41 KK77 pKa = 10.67 GPNGQALMSSTHH89 pKa = 7.21 DD90 pKa = 3.65 FTLMPSWLKK99 pKa = 10.84 NKK101 pKa = 9.77 LMSLAGVGLKK111 pKa = 9.85 QCFDD115 pKa = 5.1 LIDD118 pKa = 4.04 SVSMNGISVNSWWNRR133 pKa = 11.84 IYY135 pKa = 10.32 PVRR138 pKa = 11.84 SSTISKK144 pKa = 8.41 LTGMSDD150 pKa = 3.45 KK151 pKa = 10.8 EE152 pKa = 4.46 GKK154 pKa = 7.96 TSVMGVVDD162 pKa = 3.97 YY163 pKa = 10.35 WSQTALKK170 pKa = 8.66 PLHH173 pKa = 6.41 DD174 pKa = 3.71 TLMRR178 pKa = 11.84 ILSGIGPDD186 pKa = 3.79 CTHH189 pKa = 7.09 DD190 pKa = 3.44 QQSFLTKK197 pKa = 9.89 VPSSGVTFYY206 pKa = 11.21 SYY208 pKa = 11.81 DD209 pKa = 3.41 LTNATDD215 pKa = 5.34 SMPLWLQGEE224 pKa = 4.61 IISRR228 pKa = 11.84 MIGKK232 pKa = 9.22 DD233 pKa = 3.11 RR234 pKa = 11.84 AEE236 pKa = 4.01 DD237 pKa = 3.29 WKK239 pKa = 11.19 DD240 pKa = 3.11 ILVKK244 pKa = 10.93 EE245 pKa = 4.14 EE246 pKa = 4.46 FSLKK250 pKa = 10.1 GHH252 pKa = 5.72 PAKK255 pKa = 10.47 IKK257 pKa = 10.37 FSAGQPMGAYY267 pKa = 10.15 SSWPSMALTHH277 pKa = 6.21 HH278 pKa = 7.25 AIVQLAWISLGNKK291 pKa = 9.66 SPTRR295 pKa = 11.84 DD296 pKa = 3.54 YY297 pKa = 11.85 VLLGDD302 pKa = 6.2 DD303 pKa = 3.49 MVIWNDD309 pKa = 3.38 KK310 pKa = 10.56 LAMSYY315 pKa = 8.44 RR316 pKa = 11.84 TLLSSLDD323 pKa = 3.77 MPISDD328 pKa = 3.73 QKK330 pKa = 8.95 THH332 pKa = 6.42 ISGSTFEE339 pKa = 4.73 FCKK342 pKa = 10.24 SWFVNGVEE350 pKa = 4.24 VTGFSVPGLWEE361 pKa = 3.99 VSSSYY366 pKa = 11.51 SKK368 pKa = 10.75 LANFLEE374 pKa = 4.55 NQEE377 pKa = 4.1 SHH379 pKa = 5.15 GWKK382 pKa = 10.02 PSDD385 pKa = 3.55 ASAIPLIMSLYY396 pKa = 9.69 NCLGLHH402 pKa = 5.43 SQGKK406 pKa = 8.84 SVYY409 pKa = 10.1 KK410 pKa = 10.51 LYY412 pKa = 10.4 TVFQTLVKK420 pKa = 9.68 TKK422 pKa = 9.52 VTGEE426 pKa = 3.99 YY427 pKa = 9.57 STMAKK432 pKa = 10.65 AMTEE436 pKa = 3.82 AFRR439 pKa = 11.84 WPFYY443 pKa = 8.21 EE444 pKa = 3.93 TSNEE448 pKa = 3.54 IFASILGKK456 pKa = 9.84 MKK458 pKa = 10.52 EE459 pKa = 4.05 EE460 pKa = 3.98 QVWKK464 pKa = 10.71 DD465 pKa = 3.45 MEE467 pKa = 4.41 SLQTNVYY474 pKa = 10.02 LFHH477 pKa = 8.04 DD478 pKa = 4.51 EE479 pKa = 4.11 FNKK482 pKa = 10.33 RR483 pKa = 11.84 MSTVNPLWASEE494 pKa = 4.4 SYY496 pKa = 8.59 KK497 pKa = 9.71 TAYY500 pKa = 8.48 TEE502 pKa = 3.95 RR503 pKa = 11.84 VPMLIMAEE511 pKa = 4.13 YY512 pKa = 8.96 LTMEE516 pKa = 4.58 LEE518 pKa = 4.35 EE519 pKa = 5.01 KK520 pKa = 10.99 LMILTGDD527 pKa = 3.66 NSSDD531 pKa = 3.45 EE532 pKa = 4.12 YY533 pKa = 11.42 QEE535 pKa = 5.79 LIFNEE540 pKa = 4.23 SLGSYY545 pKa = 9.84 KK546 pKa = 10.4 LSKK549 pKa = 10.65 HH550 pKa = 5.26 FFGLKK555 pKa = 10.35 DD556 pKa = 3.41 EE557 pKa = 4.83 ASSVRR562 pKa = 11.84 DD563 pKa = 3.14 LSRR566 pKa = 11.84 LVKK569 pKa = 10.1 PLINKK574 pKa = 9.2 VSEE577 pKa = 4.8 IINKK581 pKa = 9.58 SDD583 pKa = 3.13 PTTSVILTTTEE594 pKa = 3.58 QMFVKK599 pKa = 9.87 DD600 pKa = 3.54 TATFGPP606 pKa = 4.18
Molecular weight: 68.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.617
IPC2_protein 5.563
IPC_protein 5.601
Toseland 5.97
ProMoST 5.906
Dawson 5.804
Bjellqvist 5.83
Wikipedia 5.792
Rodwell 5.779
Grimsley 6.135
Solomon 5.804
Lehninger 5.792
Nozaki 6.046
DTASelect 6.237
Thurlkill 6.211
EMBOSS 6.173
Sillero 6.148
Patrickios 4.164
IPC_peptide 5.817
IPC2_peptide 6.135
IPC2.peptide.svr19 6.127
Protein with the highest isoelectric point:
>tr|A0A1L3KIK7|A0A1L3KIK7_9VIRU RNA-dependent RNA polymerase OS=Shahe narna-like virus 7 OX=1923435 PE=4 SV=1
MM1 pKa = 7.68 PSWLFTSHH9 pKa = 5.88 EE10 pKa = 4.32 SVRR13 pKa = 11.84 IGMTVLTISSGFNLPVSVNLDD34 pKa = 3.81 PIMTPSKK41 pKa = 10.39 SIPSSMEE48 pKa = 3.11 SWEE51 pKa = 3.82 IDD53 pKa = 3.63 LVCRR57 pKa = 11.84 FLGIKK62 pKa = 9.68 EE63 pKa = 4.62 EE64 pKa = 4.14 YY65 pKa = 10.36 VEE67 pKa = 3.91 WFMFHH72 pKa = 7.14 HH73 pKa = 5.95 STKK76 pKa = 10.41 KK77 pKa = 10.67 GPNGQALMSSTHH89 pKa = 7.21 DD90 pKa = 3.65 FTLMPSWLKK99 pKa = 10.84 NKK101 pKa = 9.77 LMSLAGVGLKK111 pKa = 9.85 QCFDD115 pKa = 5.1 LIDD118 pKa = 4.04 SVSMNGISVNSWWNRR133 pKa = 11.84 IYY135 pKa = 10.32 PVRR138 pKa = 11.84 SSTISKK144 pKa = 8.41 LTGMSDD150 pKa = 3.45 KK151 pKa = 10.8 EE152 pKa = 4.46 GKK154 pKa = 7.96 TSVMGVVDD162 pKa = 3.97 YY163 pKa = 10.35 WSQTALKK170 pKa = 8.66 PLHH173 pKa = 6.41 DD174 pKa = 3.71 TLMRR178 pKa = 11.84 ILSGIGPDD186 pKa = 3.79 CTHH189 pKa = 7.09 DD190 pKa = 3.44 QQSFLTKK197 pKa = 9.89 VPSSGVTFYY206 pKa = 11.21 SYY208 pKa = 11.81 DD209 pKa = 3.41 LTNATDD215 pKa = 5.34 SMPLWLQGEE224 pKa = 4.61 IISRR228 pKa = 11.84 MIGKK232 pKa = 9.22 DD233 pKa = 3.11 RR234 pKa = 11.84 AEE236 pKa = 4.01 DD237 pKa = 3.29 WKK239 pKa = 11.19 DD240 pKa = 3.11 ILVKK244 pKa = 10.93 EE245 pKa = 4.14 EE246 pKa = 4.46 FSLKK250 pKa = 10.1 GHH252 pKa = 5.72 PAKK255 pKa = 10.47 IKK257 pKa = 10.37 FSAGQPMGAYY267 pKa = 10.15 SSWPSMALTHH277 pKa = 6.21 HH278 pKa = 7.25 AIVQLAWISLGNKK291 pKa = 9.66 SPTRR295 pKa = 11.84 DD296 pKa = 3.54 YY297 pKa = 11.85 VLLGDD302 pKa = 6.2 DD303 pKa = 3.49 MVIWNDD309 pKa = 3.38 KK310 pKa = 10.56 LAMSYY315 pKa = 8.44 RR316 pKa = 11.84 TLLSSLDD323 pKa = 3.77 MPISDD328 pKa = 3.73 QKK330 pKa = 8.95 THH332 pKa = 6.42 ISGSTFEE339 pKa = 4.73 FCKK342 pKa = 10.24 SWFVNGVEE350 pKa = 4.24 VTGFSVPGLWEE361 pKa = 3.99 VSSSYY366 pKa = 11.51 SKK368 pKa = 10.75 LANFLEE374 pKa = 4.55 NQEE377 pKa = 4.1 SHH379 pKa = 5.15 GWKK382 pKa = 10.02 PSDD385 pKa = 3.55 ASAIPLIMSLYY396 pKa = 9.69 NCLGLHH402 pKa = 5.43 SQGKK406 pKa = 8.84 SVYY409 pKa = 10.1 KK410 pKa = 10.51 LYY412 pKa = 10.4 TVFQTLVKK420 pKa = 9.68 TKK422 pKa = 9.52 VTGEE426 pKa = 3.99 YY427 pKa = 9.57 STMAKK432 pKa = 10.65 AMTEE436 pKa = 3.82 AFRR439 pKa = 11.84 WPFYY443 pKa = 8.21 EE444 pKa = 3.93 TSNEE448 pKa = 3.54 IFASILGKK456 pKa = 9.84 MKK458 pKa = 10.52 EE459 pKa = 4.05 EE460 pKa = 3.98 QVWKK464 pKa = 10.71 DD465 pKa = 3.45 MEE467 pKa = 4.41 SLQTNVYY474 pKa = 10.02 LFHH477 pKa = 8.04 DD478 pKa = 4.51 EE479 pKa = 4.11 FNKK482 pKa = 10.33 RR483 pKa = 11.84 MSTVNPLWASEE494 pKa = 4.4 SYY496 pKa = 8.59 KK497 pKa = 9.71 TAYY500 pKa = 8.48 TEE502 pKa = 3.95 RR503 pKa = 11.84 VPMLIMAEE511 pKa = 4.13 YY512 pKa = 8.96 LTMEE516 pKa = 4.58 LEE518 pKa = 4.35 EE519 pKa = 5.01 KK520 pKa = 10.99 LMILTGDD527 pKa = 3.66 NSSDD531 pKa = 3.45 EE532 pKa = 4.12 YY533 pKa = 11.42 QEE535 pKa = 5.79 LIFNEE540 pKa = 4.23 SLGSYY545 pKa = 9.84 KK546 pKa = 10.4 LSKK549 pKa = 10.65 HH550 pKa = 5.26 FFGLKK555 pKa = 10.35 DD556 pKa = 3.41 EE557 pKa = 4.83 ASSVRR562 pKa = 11.84 DD563 pKa = 3.14 LSRR566 pKa = 11.84 LVKK569 pKa = 10.1 PLINKK574 pKa = 9.2 VSEE577 pKa = 4.8 IINKK581 pKa = 9.58 SDD583 pKa = 3.13 PTTSVILTTTEE594 pKa = 3.58 QMFVKK599 pKa = 9.87 DD600 pKa = 3.54 TATFGPP606 pKa = 4.18
Molecular weight: 68.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.617
IPC2_protein 5.563
IPC_protein 5.601
Toseland 5.97
ProMoST 5.906
Dawson 5.804
Bjellqvist 5.83
Wikipedia 5.792
Rodwell 5.779
Grimsley 6.135
Solomon 5.804
Lehninger 5.792
Nozaki 6.046
DTASelect 6.237
Thurlkill 6.211
EMBOSS 6.173
Sillero 6.148
Patrickios 4.164
IPC_peptide 5.817
IPC2_peptide 6.135
IPC2.peptide.svr19 6.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
606
606
606
606.0
68.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.96 ± 0.0
0.825 ± 0.0
4.95 ± 0.0
5.941 ± 0.0
4.455 ± 0.0
5.611 ± 0.0
2.31 ± 0.0
5.446 ± 0.0
6.931 ± 0.0
9.736 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.95 ± 0.0
3.63 ± 0.0
4.29 ± 0.0
2.64 ± 0.0
2.31 ± 0.0
12.541 ± 0.0
7.096 ± 0.0
6.106 ± 0.0
2.97 ± 0.0
3.3 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here