Listeria phage PSU-VKH-LP040

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UUU2|A0A2U8UUU2_9CAUD DnaD domain protein OS=Listeria phage PSU-VKH-LP040 OX=2202247 PE=4 SV=1
MM1 pKa = 7.45TKK3 pKa = 9.88QEE5 pKa = 4.21SVNCKK10 pKa = 9.98EE11 pKa = 4.1CDD13 pKa = 3.45EE14 pKa = 4.87TFTQYY19 pKa = 11.66DD20 pKa = 4.01EE21 pKa = 5.86IIVFDD26 pKa = 3.95GNAWHH31 pKa = 7.41KK32 pKa = 11.18DD33 pKa = 3.58CLDD36 pKa = 4.09KK37 pKa = 11.34FPATYY42 pKa = 10.07AYY44 pKa = 10.38YY45 pKa = 10.54EE46 pKa = 4.15KK47 pKa = 11.15GKK49 pKa = 10.44DD50 pKa = 3.61GEE52 pKa = 4.25FLNTGEE58 pKa = 4.47HH59 pKa = 7.09ADD61 pKa = 3.95FEE63 pKa = 5.74GDD65 pKa = 3.33AYY67 pKa = 10.63EE68 pKa = 4.92HH69 pKa = 6.91FDD71 pKa = 3.84GLEE74 pKa = 3.88EE75 pKa = 4.03EE76 pKa = 4.61

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UUZ1|A0A2U8UUZ1_9CAUD Recombinational DNA repair protein OS=Listeria phage PSU-VKH-LP040 OX=2202247 PE=4 SV=1
MM1 pKa = 7.58LSEE4 pKa = 4.15KK5 pKa = 10.52AKK7 pKa = 9.43EE8 pKa = 3.8ARR10 pKa = 11.84RR11 pKa = 11.84VYY13 pKa = 8.89QQQWRR18 pKa = 11.84DD19 pKa = 3.15KK20 pKa = 9.18NRR22 pKa = 11.84EE23 pKa = 3.69HH24 pKa = 7.2VRR26 pKa = 11.84EE27 pKa = 4.14YY28 pKa = 9.54SRR30 pKa = 11.84KK31 pKa = 7.94WRR33 pKa = 11.84EE34 pKa = 3.59EE35 pKa = 3.75NQEE38 pKa = 4.03KK39 pKa = 10.32QEE41 pKa = 4.0AAINRR46 pKa = 11.84YY47 pKa = 7.15WEE49 pKa = 4.05RR50 pKa = 11.84KK51 pKa = 9.08ANEE54 pKa = 4.42LIANN58 pKa = 4.58

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12180

48

1788

181.8

20.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.079 ± 1.096

0.681 ± 0.11

5.878 ± 0.376

7.931 ± 0.604

3.793 ± 0.245

5.739 ± 0.458

1.182 ± 0.183

6.814 ± 0.33

9.475 ± 0.378

8.259 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.152

6.396 ± 0.297

2.438 ± 0.233

3.678 ± 0.193

3.571 ± 0.44

6.297 ± 0.455

5.92 ± 0.244

6.478 ± 0.264

1.067 ± 0.151

3.834 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski