Streptococcus satellite phage Javan727

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZUW6|A0A4D5ZUW6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan727 OX=2558821 GN=JavanS727_0023 PE=4 SV=1
MM1 pKa = 7.87EE2 pKa = 4.51EE3 pKa = 3.56TTYY6 pKa = 11.03DD7 pKa = 3.82IIAKK11 pKa = 9.74SLDD14 pKa = 4.0RR15 pKa = 11.84ISMEE19 pKa = 3.74LHH21 pKa = 5.91QADD24 pKa = 4.33EE25 pKa = 4.59NNDD28 pKa = 3.14FLEE31 pKa = 4.3YY32 pKa = 10.95GFYY35 pKa = 10.31QDD37 pKa = 3.87NN38 pKa = 3.58

Molecular weight: 4.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZUW0|A0A4D5ZUW0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan727 OX=2558821 GN=JavanS727_0016 PE=4 SV=1
MM1 pKa = 7.38GKK3 pKa = 9.59SATNMKK9 pKa = 10.18RR10 pKa = 11.84IATIFLLGLPKK21 pKa = 9.96VPKK24 pKa = 8.88MRR26 pKa = 11.84PIAYY30 pKa = 8.55PRR32 pKa = 11.84ATISNNPEE40 pKa = 3.14ARR42 pKa = 11.84GIYY45 pKa = 10.11LEE47 pKa = 4.06NKK49 pKa = 9.02KK50 pKa = 10.62

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

3851

38

492

148.1

17.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.375 ± 0.21

0.831 ± 0.161

5.895 ± 0.451

8.517 ± 0.533

4.051 ± 0.288

5.09 ± 0.327

1.532 ± 0.225

6.57 ± 0.4

9.14 ± 0.528

9.894 ± 0.478

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.856 ± 0.3

5.635 ± 0.468

3.376 ± 0.465

3.973 ± 0.319

5.505 ± 0.379

5.09 ± 0.4

6.388 ± 0.339

5.012 ± 0.366

0.857 ± 0.168

4.414 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski