Potato mop-top virus (isolate Potato/Sweden/Sw) (PMTV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Pomovirus; Potato mop-top virus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9IV53|TGB2_PMTVS Movement protein TGB2 OS=Potato mop-top virus (isolate Potato/Sweden/Sw) OX=652839 PE=1 SV=1
MM1 pKa = 8.07DD2 pKa = 5.64PPVILHH8 pKa = 6.49SPNCSCQFCSSEE20 pKa = 4.21LPSTHH25 pKa = 6.27TCGSQDD31 pKa = 2.88RR32 pKa = 11.84TVPLHH37 pKa = 6.32VEE39 pKa = 4.07ATAAGHH45 pKa = 5.8MEE47 pKa = 4.3AKK49 pKa = 10.27NFSLQYY55 pKa = 8.82VLLVAFVSVLLGFSFCVYY73 pKa = 10.03LKK75 pKa = 11.15SMSNDD80 pKa = 3.03EE81 pKa = 4.61ASDD84 pKa = 3.29MTYY87 pKa = 10.73YY88 pKa = 10.85YY89 pKa = 10.77QDD91 pKa = 3.82LNSVEE96 pKa = 4.15IKK98 pKa = 10.47LGKK101 pKa = 10.1NPLDD105 pKa = 3.71PEE107 pKa = 4.51VIKK110 pKa = 10.69AIHH113 pKa = 6.22SFQEE117 pKa = 4.18FPYY120 pKa = 10.9GNIPSIRR127 pKa = 11.84RR128 pKa = 11.84EE129 pKa = 4.08AEE131 pKa = 3.35FDD133 pKa = 3.75VQNDD137 pKa = 3.22EE138 pKa = 4.2SSAVVLSGSNNNRR151 pKa = 11.84RR152 pKa = 11.84QVASTPCEE160 pKa = 3.87NNVLLKK166 pKa = 10.41LWKK169 pKa = 9.97DD170 pKa = 3.56DD171 pKa = 4.14LSFTIIAVTVLVGAMLARR189 pKa = 11.84CC190 pKa = 4.07

Molecular weight: 21.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9IV52|TGB3_PMTVS Movement protein TGB3 OS=Potato mop-top virus (isolate Potato/Sweden/Sw) OX=652839 PE=1 SV=1
MM1 pKa = 7.36EE2 pKa = 4.88RR3 pKa = 11.84RR4 pKa = 11.84FIVYY8 pKa = 7.43YY9 pKa = 10.4NCSDD13 pKa = 4.15CACGRR18 pKa = 11.84YY19 pKa = 9.28VSSLLTMSGAYY30 pKa = 10.46VNVCVCIVFFILVYY44 pKa = 10.76LSSCYY49 pKa = 10.44NMRR52 pKa = 11.84VLGFLRR58 pKa = 11.84VCIYY62 pKa = 10.56LYY64 pKa = 10.62KK65 pKa = 10.53LCRR68 pKa = 3.58

Molecular weight: 7.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3653

68

1812

521.9

58.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.035 ± 1.022

2.053 ± 0.609

5.886 ± 0.718

7.282 ± 0.732

3.942 ± 0.452

6.488 ± 0.746

1.588 ± 0.12

4.9 ± 0.334

6.022 ± 1.209

9.143 ± 0.473

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.429

5.174 ± 0.561

3.23 ± 0.482

3.641 ± 0.285

6.406 ± 0.826

7.911 ± 0.606

4.708 ± 0.214

7.72 ± 0.41

1.04 ± 0.152

3.531 ± 0.526
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski