Cellulophaga phage phi38:1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0A1M1|S0A1M1_9CAUD Uncharacterized protein OS=Cellulophaga phage phi38:1 OX=1327977 GN=Phi38:1_gp068 PE=4 SV=1
MM1 pKa = 7.73SDD3 pKa = 3.44KK4 pKa = 11.07LLGAIEE10 pKa = 4.76LKK12 pKa = 9.83TIRR15 pKa = 11.84IDD17 pKa = 3.57TVFYY21 pKa = 10.5DD22 pKa = 5.02YY23 pKa = 11.2EE24 pKa = 4.55GQDD27 pKa = 3.43LGDD30 pKa = 3.63EE31 pKa = 4.38EE32 pKa = 4.56VRR34 pKa = 11.84EE35 pKa = 4.35SILGGDD41 pKa = 3.74YY42 pKa = 11.05GAVSVYY48 pKa = 10.88VNDD51 pKa = 3.77VLASSYY57 pKa = 11.12GDD59 pKa = 3.71YY60 pKa = 10.79YY61 pKa = 10.91HH62 pKa = 7.48EE63 pKa = 5.25KK64 pKa = 10.81GDD66 pKa = 3.65VAADD70 pKa = 3.91AFASGLLFALNAPEE84 pKa = 4.83DD85 pKa = 4.03FEE87 pKa = 4.84ILYY90 pKa = 9.33TNSIEE95 pKa = 4.41VYY97 pKa = 10.27

Molecular weight: 10.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9ZY54|R9ZY54_9CAUD Uncharacterized protein OS=Cellulophaga phage phi38:1 OX=1327977 GN=Phi38:1_gp011 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.37GIRR5 pKa = 11.84LNTPYY10 pKa = 10.35TKK12 pKa = 10.36AYY14 pKa = 10.16DD15 pKa = 3.86GEE17 pKa = 4.65GNLINEE23 pKa = 4.13ILFGYY28 pKa = 10.17RR29 pKa = 11.84SLGPNRR35 pKa = 11.84RR36 pKa = 11.84QRR38 pKa = 11.84RR39 pKa = 11.84IDD41 pKa = 3.38SGTSSSRR48 pKa = 11.84PFSNKK53 pKa = 9.07KK54 pKa = 9.43GRR56 pKa = 11.84QIVITKK62 pKa = 9.08YY63 pKa = 11.01GPFHH67 pKa = 6.29FLKK70 pKa = 9.57TEE72 pKa = 3.72KK73 pKa = 10.44VVRR76 pKa = 11.84QGYY79 pKa = 9.97KK80 pKa = 8.1NTRR83 pKa = 11.84LEE85 pKa = 4.2VKK87 pKa = 10.01ILNN90 pKa = 3.92

Molecular weight: 10.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

22005

33

1691

217.9

24.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.817 ± 0.327

0.936 ± 0.124

6.912 ± 0.196

7.566 ± 0.29

4.035 ± 0.138

7.112 ± 0.303

1.377 ± 0.125

6.848 ± 0.188

8.466 ± 0.44

8.071 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.313 ± 0.123

5.285 ± 0.243

2.904 ± 0.19

2.681 ± 0.149

4.485 ± 0.139

7.648 ± 0.361

5.667 ± 0.275

6.344 ± 0.211

1.054 ± 0.102

4.476 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski