Amino acid dipepetide frequency for Bacillus phage SPG24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.62AlaAla: 3.62 ± 0.793
0.226AlaCys: 0.226 ± 0.23
4.072AlaAsp: 4.072 ± 1.519
4.072AlaGlu: 4.072 ± 1.057
1.357AlaPhe: 1.357 ± 0.636
3.62AlaGly: 3.62 ± 0.939
1.81AlaHis: 1.81 ± 0.835
3.167AlaIle: 3.167 ± 1.052
4.751AlaLys: 4.751 ± 1.025
5.204AlaLeu: 5.204 ± 0.958
1.357AlaMet: 1.357 ± 0.627
1.81AlaAsn: 1.81 ± 0.546
1.584AlaPro: 1.584 ± 0.583
3.167AlaGln: 3.167 ± 0.927
2.262AlaArg: 2.262 ± 0.596
3.62AlaSer: 3.62 ± 1.042
2.941AlaThr: 2.941 ± 0.82
2.715AlaVal: 2.715 ± 0.78
0.0AlaTrp: 0.0 ± 0.072
1.584AlaTyr: 1.584 ± 0.561
0.0AlaXaa: 0.0 ± 0.0
Cys
0.452CysAla: 0.452 ± 0.307
0.226CysCys: 0.226 ± 0.278
0.679CysAsp: 0.679 ± 0.356
0.226CysGlu: 0.226 ± 0.176
0.452CysPhe: 0.452 ± 0.312
0.679CysGly: 0.679 ± 0.313
0.226CysHis: 0.226 ± 0.278
1.357CysIle: 1.357 ± 0.711
0.679CysLys: 0.679 ± 0.439
0.679CysLeu: 0.679 ± 0.43
0.452CysMet: 0.452 ± 0.297
0.226CysAsn: 0.226 ± 0.263
1.131CysPro: 1.131 ± 0.42
0.0CysGln: 0.0 ± 0.0
1.131CysArg: 1.131 ± 0.63
1.131CysSer: 1.131 ± 0.57
0.0CysThr: 0.0 ± 0.0
0.452CysVal: 0.452 ± 0.316
0.452CysTrp: 0.452 ± 0.329
0.679CysTyr: 0.679 ± 0.365
0.0CysXaa: 0.0 ± 0.0
Asp
2.262AspAla: 2.262 ± 0.682
0.452AspCys: 0.452 ± 0.356
3.846AspAsp: 3.846 ± 0.775
3.394AspGlu: 3.394 ± 0.739
3.846AspPhe: 3.846 ± 1.184
2.489AspGly: 2.489 ± 1.056
0.452AspHis: 0.452 ± 0.354
2.262AspIle: 2.262 ± 0.707
4.977AspLys: 4.977 ± 1.191
6.335AspLeu: 6.335 ± 1.546
1.584AspMet: 1.584 ± 0.534
2.036AspAsn: 2.036 ± 0.883
1.584AspPro: 1.584 ± 0.704
0.905AspGln: 0.905 ± 0.581
2.262AspArg: 2.262 ± 0.563
3.62AspSer: 3.62 ± 1.312
4.525AspThr: 4.525 ± 1.164
5.204AspVal: 5.204 ± 0.831
0.452AspTrp: 0.452 ± 0.305
2.941AspTyr: 2.941 ± 0.558
0.0AspXaa: 0.0 ± 0.0
Glu
4.299GluAla: 4.299 ± 0.917
0.226GluCys: 0.226 ± 0.177
3.846GluAsp: 3.846 ± 0.532
7.919GluGlu: 7.919 ± 1.338
2.262GluPhe: 2.262 ± 1.07
3.62GluGly: 3.62 ± 0.779
2.036GluHis: 2.036 ± 0.449
4.751GluIle: 4.751 ± 0.832
6.335GluLys: 6.335 ± 1.05
5.204GluLeu: 5.204 ± 1.257
1.131GluMet: 1.131 ± 0.469
4.072GluAsn: 4.072 ± 0.839
2.489GluPro: 2.489 ± 0.803
2.941GluGln: 2.941 ± 0.801
3.846GluArg: 3.846 ± 1.052
2.941GluSer: 2.941 ± 0.553
3.846GluThr: 3.846 ± 0.978
4.751GluVal: 4.751 ± 1.134
0.905GluTrp: 0.905 ± 0.6
1.584GluTyr: 1.584 ± 0.469
0.0GluXaa: 0.0 ± 0.0
Phe
2.262PheAla: 2.262 ± 0.761
0.679PheCys: 0.679 ± 0.348
3.167PheAsp: 3.167 ± 0.701
2.489PheGlu: 2.489 ± 0.669
1.357PhePhe: 1.357 ± 0.63
1.81PheGly: 1.81 ± 0.595
1.131PheHis: 1.131 ± 0.661
3.62PheIle: 3.62 ± 1.124
3.167PheLys: 3.167 ± 0.932
3.167PheLeu: 3.167 ± 0.869
0.679PheMet: 0.679 ± 0.457
2.262PheAsn: 2.262 ± 0.845
1.584PhePro: 1.584 ± 0.661
1.131PheGln: 1.131 ± 0.466
1.81PheArg: 1.81 ± 0.616
3.394PheSer: 3.394 ± 0.74
1.81PheThr: 1.81 ± 0.509
2.489PheVal: 2.489 ± 0.655
0.226PheTrp: 0.226 ± 0.232
2.941PheTyr: 2.941 ± 1.061
0.0PheXaa: 0.0 ± 0.0
Gly
1.81GlyAla: 1.81 ± 0.378
0.679GlyCys: 0.679 ± 0.449
3.394GlyAsp: 3.394 ± 0.972
3.167GlyGlu: 3.167 ± 0.741
1.81GlyPhe: 1.81 ± 0.426
3.846GlyGly: 3.846 ± 0.767
1.131GlyHis: 1.131 ± 0.447
2.715GlyIle: 2.715 ± 0.829
5.204GlyLys: 5.204 ± 1.838
2.941GlyLeu: 2.941 ± 1.145
1.131GlyMet: 1.131 ± 0.434
4.299GlyAsn: 4.299 ± 1.036
0.679GlyPro: 0.679 ± 0.406
2.489GlyGln: 2.489 ± 0.803
3.62GlyArg: 3.62 ± 0.895
4.751GlySer: 4.751 ± 0.675
3.846GlyThr: 3.846 ± 1.055
4.072GlyVal: 4.072 ± 0.777
0.452GlyTrp: 0.452 ± 0.359
1.357GlyTyr: 1.357 ± 0.55
0.0GlyXaa: 0.0 ± 0.0
His
1.131HisAla: 1.131 ± 0.371
0.452HisCys: 0.452 ± 0.307
0.452HisAsp: 0.452 ± 0.289
0.679HisGlu: 0.679 ± 0.426
1.131HisPhe: 1.131 ± 0.616
1.131HisGly: 1.131 ± 0.432
0.452HisHis: 0.452 ± 0.284
2.036HisIle: 2.036 ± 0.592
1.584HisLys: 1.584 ± 0.776
2.036HisLeu: 2.036 ± 0.675
0.226HisMet: 0.226 ± 0.224
0.905HisAsn: 0.905 ± 0.386
0.452HisPro: 0.452 ± 0.255
0.679HisGln: 0.679 ± 0.314
0.679HisArg: 0.679 ± 0.374
1.81HisSer: 1.81 ± 0.785
2.262HisThr: 2.262 ± 0.71
2.036HisVal: 2.036 ± 0.718
0.226HisTrp: 0.226 ± 0.237
0.679HisTyr: 0.679 ± 0.372
0.0HisXaa: 0.0 ± 0.0
Ile
2.715IleAla: 2.715 ± 0.959
0.452IleCys: 0.452 ± 0.369
3.167IleAsp: 3.167 ± 0.836
3.394IleGlu: 3.394 ± 0.658
1.357IlePhe: 1.357 ± 0.444
2.262IleGly: 2.262 ± 0.638
0.679IleHis: 0.679 ± 0.371
3.167IleIle: 3.167 ± 0.774
4.751IleLys: 4.751 ± 1.007
5.882IleLeu: 5.882 ± 1.234
1.131IleMet: 1.131 ± 0.401
3.846IleAsn: 3.846 ± 0.891
2.036IlePro: 2.036 ± 0.466
2.715IleGln: 2.715 ± 0.681
3.62IleArg: 3.62 ± 0.801
4.072IleSer: 4.072 ± 0.799
3.846IleThr: 3.846 ± 0.812
4.072IleVal: 4.072 ± 0.598
0.226IleTrp: 0.226 ± 0.246
1.357IleTyr: 1.357 ± 0.585
0.0IleXaa: 0.0 ± 0.0
Lys
6.561LysAla: 6.561 ± 1.97
0.452LysCys: 0.452 ± 0.329
1.584LysAsp: 1.584 ± 0.367
7.466LysGlu: 7.466 ± 1.569
4.525LysPhe: 4.525 ± 0.944
5.204LysGly: 5.204 ± 0.769
1.357LysHis: 1.357 ± 0.506
3.846LysIle: 3.846 ± 0.86
7.919LysLys: 7.919 ± 1.89
4.977LysLeu: 4.977 ± 0.716
1.131LysMet: 1.131 ± 0.711
4.299LysAsn: 4.299 ± 1.177
3.846LysPro: 3.846 ± 0.723
5.43LysGln: 5.43 ± 1.269
3.394LysArg: 3.394 ± 1.125
4.525LysSer: 4.525 ± 0.917
5.204LysThr: 5.204 ± 1.088
8.145LysVal: 8.145 ± 1.462
0.452LysTrp: 0.452 ± 0.339
2.036LysTyr: 2.036 ± 0.628
0.0LysXaa: 0.0 ± 0.0
Leu
4.977LeuAla: 4.977 ± 0.735
1.131LeuCys: 1.131 ± 0.551
6.335LeuAsp: 6.335 ± 1.491
6.109LeuGlu: 6.109 ± 1.134
3.62LeuPhe: 3.62 ± 0.542
3.846LeuGly: 3.846 ± 1.01
2.036LeuHis: 2.036 ± 0.646
2.715LeuIle: 2.715 ± 0.722
8.145LeuLys: 8.145 ± 1.256
5.43LeuLeu: 5.43 ± 1.053
1.357LeuMet: 1.357 ± 0.765
3.62LeuAsn: 3.62 ± 1.006
3.167LeuPro: 3.167 ± 0.857
4.299LeuGln: 4.299 ± 0.973
4.072LeuArg: 4.072 ± 0.955
6.787LeuSer: 6.787 ± 1.284
7.692LeuThr: 7.692 ± 1.568
5.43LeuVal: 5.43 ± 0.92
0.905LeuTrp: 0.905 ± 0.51
4.299LeuTyr: 4.299 ± 0.734
0.0LeuXaa: 0.0 ± 0.0
Met
1.357MetAla: 1.357 ± 0.531
0.452MetCys: 0.452 ± 0.242
0.679MetAsp: 0.679 ± 0.467
2.036MetGlu: 2.036 ± 0.875
0.679MetPhe: 0.679 ± 0.331
0.452MetGly: 0.452 ± 0.275
0.452MetHis: 0.452 ± 0.332
0.905MetIle: 0.905 ± 0.317
1.357MetLys: 1.357 ± 0.588
1.584MetLeu: 1.584 ± 0.658
0.679MetMet: 0.679 ± 0.371
1.131MetAsn: 1.131 ± 0.649
0.452MetPro: 0.452 ± 0.288
0.679MetGln: 0.679 ± 0.369
0.679MetArg: 0.679 ± 0.43
1.81MetSer: 1.81 ± 0.87
1.357MetThr: 1.357 ± 0.51
0.905MetVal: 0.905 ± 0.374
0.226MetTrp: 0.226 ± 0.23
1.131MetTyr: 1.131 ± 0.486
0.0MetXaa: 0.0 ± 0.0
Asn
2.489AsnAla: 2.489 ± 0.656
0.452AsnCys: 0.452 ± 0.422
1.584AsnAsp: 1.584 ± 0.603
1.584AsnGlu: 1.584 ± 0.64
3.167AsnPhe: 3.167 ± 0.57
3.167AsnGly: 3.167 ± 1.087
0.679AsnHis: 0.679 ± 0.399
2.489AsnIle: 2.489 ± 0.483
5.656AsnLys: 5.656 ± 1.258
3.394AsnLeu: 3.394 ± 0.714
0.905AsnMet: 0.905 ± 0.449
4.072AsnAsn: 4.072 ± 1.054
1.584AsnPro: 1.584 ± 0.739
2.036AsnGln: 2.036 ± 0.673
2.941AsnArg: 2.941 ± 0.809
2.941AsnSer: 2.941 ± 0.642
5.882AsnThr: 5.882 ± 0.821
2.941AsnVal: 2.941 ± 0.646
0.452AsnTrp: 0.452 ± 0.291
2.036AsnTyr: 2.036 ± 0.524
0.0AsnXaa: 0.0 ± 0.0
Pro
1.131ProAla: 1.131 ± 0.43
0.679ProCys: 0.679 ± 0.495
2.262ProAsp: 2.262 ± 0.524
1.81ProGlu: 1.81 ± 0.631
1.357ProPhe: 1.357 ± 0.548
1.131ProGly: 1.131 ± 0.476
0.226ProHis: 0.226 ± 0.224
2.262ProIle: 2.262 ± 0.639
2.715ProLys: 2.715 ± 0.873
4.525ProLeu: 4.525 ± 0.982
0.679ProMet: 0.679 ± 0.373
1.357ProAsn: 1.357 ± 0.473
1.357ProPro: 1.357 ± 0.595
1.584ProGln: 1.584 ± 0.408
1.81ProArg: 1.81 ± 0.651
0.905ProSer: 0.905 ± 0.458
3.394ProThr: 3.394 ± 1.122
1.81ProVal: 1.81 ± 0.674
0.452ProTrp: 0.452 ± 0.294
1.81ProTyr: 1.81 ± 0.664
0.0ProXaa: 0.0 ± 0.0
Gln
1.357GlnAla: 1.357 ± 0.638
0.679GlnCys: 0.679 ± 0.348
2.715GlnAsp: 2.715 ± 0.717
4.751GlnGlu: 4.751 ± 0.912
2.262GlnPhe: 2.262 ± 0.736
1.81GlnGly: 1.81 ± 0.746
0.452GlnHis: 0.452 ± 0.317
2.941GlnIle: 2.941 ± 0.743
3.167GlnLys: 3.167 ± 0.813
3.846GlnLeu: 3.846 ± 0.999
1.131GlnMet: 1.131 ± 0.429
2.489GlnAsn: 2.489 ± 0.567
1.131GlnPro: 1.131 ± 0.629
3.846GlnGln: 3.846 ± 1.318
2.489GlnArg: 2.489 ± 0.661
3.846GlnSer: 3.846 ± 0.878
1.81GlnThr: 1.81 ± 0.645
2.262GlnVal: 2.262 ± 0.551
0.0GlnTrp: 0.0 ± 0.031
2.489GlnTyr: 2.489 ± 0.913
0.0GlnXaa: 0.0 ± 0.0
Arg
4.072ArgAla: 4.072 ± 0.963
0.905ArgCys: 0.905 ± 0.464
2.941ArgAsp: 2.941 ± 0.763
1.81ArgGlu: 1.81 ± 0.635
3.167ArgPhe: 3.167 ± 1.047
3.846ArgGly: 3.846 ± 0.942
2.036ArgHis: 2.036 ± 0.665
3.167ArgIle: 3.167 ± 0.801
3.167ArgLys: 3.167 ± 1.058
4.977ArgLeu: 4.977 ± 1.001
0.905ArgMet: 0.905 ± 0.437
2.262ArgAsn: 2.262 ± 0.834
0.679ArgPro: 0.679 ± 0.42
2.262ArgGln: 2.262 ± 0.744
3.846ArgArg: 3.846 ± 1.251
3.394ArgSer: 3.394 ± 0.991
4.072ArgThr: 4.072 ± 0.974
3.394ArgVal: 3.394 ± 1.003
0.905ArgTrp: 0.905 ± 0.429
4.072ArgTyr: 4.072 ± 1.139
0.0ArgXaa: 0.0 ± 0.0
Ser
2.941SerAla: 2.941 ± 0.746
1.131SerCys: 1.131 ± 0.558
4.072SerAsp: 4.072 ± 0.847
4.299SerGlu: 4.299 ± 1.124
1.81SerPhe: 1.81 ± 0.575
4.072SerGly: 4.072 ± 0.958
1.131SerHis: 1.131 ± 0.484
4.072SerIle: 4.072 ± 1.119
4.525SerLys: 4.525 ± 1.012
7.24SerLeu: 7.24 ± 1.351
1.584SerMet: 1.584 ± 0.605
2.715SerAsn: 2.715 ± 0.771
1.131SerPro: 1.131 ± 0.537
2.036SerGln: 2.036 ± 0.774
5.882SerArg: 5.882 ± 1.46
7.466SerSer: 7.466 ± 1.331
5.43SerThr: 5.43 ± 1.941
4.072SerVal: 4.072 ± 1.368
0.905SerTrp: 0.905 ± 0.383
3.167SerTyr: 3.167 ± 0.779
0.0SerXaa: 0.0 ± 0.0
Thr
4.072ThrAla: 4.072 ± 0.669
0.452ThrCys: 0.452 ± 0.363
4.525ThrAsp: 4.525 ± 1.229
6.109ThrGlu: 6.109 ± 1.547
1.584ThrPhe: 1.584 ± 0.5
4.525ThrGly: 4.525 ± 1.219
1.357ThrHis: 1.357 ± 0.476
3.62ThrIle: 3.62 ± 0.74
6.335ThrLys: 6.335 ± 0.982
7.919ThrLeu: 7.919 ± 2.214
0.679ThrMet: 0.679 ± 0.428
2.262ThrAsn: 2.262 ± 0.801
3.167ThrPro: 3.167 ± 1.078
3.394ThrGln: 3.394 ± 0.771
4.072ThrArg: 4.072 ± 1.06
4.072ThrSer: 4.072 ± 0.829
8.145ThrThr: 8.145 ± 3.366
7.014ThrVal: 7.014 ± 1.585
1.584ThrTrp: 1.584 ± 0.669
2.489ThrTyr: 2.489 ± 0.642
0.0ThrXaa: 0.0 ± 0.0
Val
2.941ValAla: 2.941 ± 0.851
0.452ValCys: 0.452 ± 0.278
3.167ValAsp: 3.167 ± 0.898
4.299ValGlu: 4.299 ± 0.794
4.072ValPhe: 4.072 ± 1.185
3.394ValGly: 3.394 ± 0.799
2.489ValHis: 2.489 ± 0.977
3.167ValIle: 3.167 ± 0.722
4.299ValLys: 4.299 ± 1.078
5.656ValLeu: 5.656 ± 1.669
0.905ValMet: 0.905 ± 0.418
3.394ValAsn: 3.394 ± 0.962
4.072ValPro: 4.072 ± 1.152
2.489ValGln: 2.489 ± 0.683
3.62ValArg: 3.62 ± 1.026
4.525ValSer: 4.525 ± 0.923
7.692ValThr: 7.692 ± 2.98
3.846ValVal: 3.846 ± 1.269
0.226ValTrp: 0.226 ± 0.228
3.846ValTyr: 3.846 ± 0.687
0.0ValXaa: 0.0 ± 0.0
Trp
0.226TrpAla: 0.226 ± 0.176
0.226TrpCys: 0.226 ± 0.077
0.0TrpAsp: 0.0 ± 0.0
0.905TrpGlu: 0.905 ± 0.326
0.452TrpPhe: 0.452 ± 0.299
0.226TrpGly: 0.226 ± 0.238
0.0TrpHis: 0.0 ± 0.0
0.452TrpIle: 0.452 ± 0.348
0.905TrpLys: 0.905 ± 0.371
0.905TrpLeu: 0.905 ± 0.374
0.452TrpMet: 0.452 ± 0.33
0.679TrpAsn: 0.679 ± 0.471
0.0TrpPro: 0.0 ± 0.032
0.679TrpGln: 0.679 ± 0.277
0.905TrpArg: 0.905 ± 0.521
0.452TrpSer: 0.452 ± 0.34
0.452TrpThr: 0.452 ± 0.364
0.905TrpVal: 0.905 ± 0.48
0.0TrpTrp: 0.0 ± 0.038
0.452TrpTyr: 0.452 ± 0.326
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.262TyrAla: 2.262 ± 0.597
0.905TyrCys: 0.905 ± 0.415
2.941TyrAsp: 2.941 ± 0.547
2.489TyrGlu: 2.489 ± 0.636
0.905TyrPhe: 0.905 ± 0.409
2.262TyrGly: 2.262 ± 0.741
0.905TyrHis: 0.905 ± 0.534
2.036TyrIle: 2.036 ± 0.591
2.715TyrLys: 2.715 ± 0.629
4.072TyrLeu: 4.072 ± 1.1
0.905TyrMet: 0.905 ± 0.376
2.715TyrAsn: 2.715 ± 0.916
1.131TyrPro: 1.131 ± 0.413
2.715TyrGln: 2.715 ± 0.773
2.941TyrArg: 2.941 ± 0.86
3.846TyrSer: 3.846 ± 1.257
3.167TyrThr: 3.167 ± 1.14
2.036TyrVal: 2.036 ± 0.805
0.226TyrTrp: 0.226 ± 0.266
2.489TyrTyr: 2.489 ± 1.073
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 34 proteins (4421 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski