Amino acid dipepetide frequency for Streptomyces sp. SA15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.882AlaAla: 19.882 ± 0.123	1.099AlaCys: 1.099 ± 0.024	8.297AlaAsp: 8.297 ± 0.054	8.684AlaGlu: 8.684 ± 0.081	3.446AlaPhe: 3.446 ± 0.037	12.112AlaGly: 12.112 ± 0.086	2.842AlaHis: 2.842 ± 0.035	3.542AlaIle: 3.542 ± 0.043	2.977AlaLys: 2.977 ± 0.046	14.153AlaLeu: 14.153 ± 0.093	2.501AlaMet: 2.501 ± 0.03	1.981AlaAsn: 1.981 ± 0.032	6.673AlaPro: 6.673 ± 0.065	3.806AlaGln: 3.806 ± 0.042	9.88AlaArg: 9.88 ± 0.071	5.891AlaSer: 5.891 ± 0.051	7.029AlaThr: 7.029 ± 0.063	11.909AlaVal: 11.909 ± 0.067	1.884AlaTrp: 1.884 ± 0.028	2.833AlaTyr: 2.833 ± 0.031	0.0AlaXaa: 0.0 ± 0.0
Cys	1.074CysAla: 1.074 ± 0.025	0.095CysCys: 0.095 ± 0.006	0.48CysAsp: 0.48 ± 0.014	0.423CysGlu: 0.423 ± 0.011	0.234CysPhe: 0.234 ± 0.01	0.948CysGly: 0.948 ± 0.019	0.193CysHis: 0.193 ± 0.009	0.161CysIle: 0.161 ± 0.008	0.129CysLys: 0.129 ± 0.007	0.745CysLeu: 0.745 ± 0.016	0.119CysMet: 0.119 ± 0.007	0.133CysAsn: 0.133 ± 0.007	0.502CysPro: 0.502 ± 0.014	0.173CysGln: 0.173 ± 0.007	0.626CysArg: 0.626 ± 0.017	0.423CysSer: 0.423 ± 0.013	0.522CysThr: 0.522 ± 0.014	0.706CysVal: 0.706 ± 0.017	0.136CysTrp: 0.136 ± 0.007	0.161CysTyr: 0.161 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	7.55AspAla: 7.55 ± 0.06	0.441AspCys: 0.441 ± 0.014	3.712AspAsp: 3.712 ± 0.044	3.95AspGlu: 3.95 ± 0.038	1.678AspPhe: 1.678 ± 0.024	6.426AspGly: 6.426 ± 0.058	1.442AspHis: 1.442 ± 0.023	2.012AspIle: 2.012 ± 0.03	1.351AspLys: 1.351 ± 0.029	6.233AspLeu: 6.233 ± 0.051	0.862AspMet: 0.862 ± 0.02	1.04AspAsn: 1.04 ± 0.022	4.503AspPro: 4.503 ± 0.044	1.616AspGln: 1.616 ± 0.027	4.873AspArg: 4.873 ± 0.053	2.699AspSer: 2.699 ± 0.035	3.357AspThr: 3.357 ± 0.038	4.822AspVal: 4.822 ± 0.036	1.064AspTrp: 1.064 ± 0.02	1.176AspTyr: 1.176 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.426GluAla: 7.426 ± 0.069	0.383GluCys: 0.383 ± 0.012	2.959GluAsp: 2.959 ± 0.033	3.681GluGlu: 3.681 ± 0.05	1.509GluPhe: 1.509 ± 0.023	4.421GluGly: 4.421 ± 0.042	1.555GluHis: 1.555 ± 0.025	2.272GluIle: 2.272 ± 0.028	1.557GluLys: 1.557 ± 0.027	6.905GluLeu: 6.905 ± 0.056	0.913GluMet: 0.913 ± 0.017	1.071GluAsn: 1.071 ± 0.021	3.473GluPro: 3.473 ± 0.046	2.332GluGln: 2.332 ± 0.033	5.483GluArg: 5.483 ± 0.047	2.609GluSer: 2.609 ± 0.036	2.995GluThr: 2.995 ± 0.031	4.494GluVal: 4.494 ± 0.047	0.819GluTrp: 0.819 ± 0.015	1.192GluTyr: 1.192 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.61PheAla: 3.61 ± 0.04	0.257PheCys: 0.257 ± 0.009	2.002PheAsp: 2.002 ± 0.028	1.449PheGlu: 1.449 ± 0.025	0.901PhePhe: 0.901 ± 0.021	2.969PheGly: 2.969 ± 0.039	0.62PheHis: 0.62 ± 0.014	0.726PheIle: 0.726 ± 0.017	0.557PheLys: 0.557 ± 0.016	2.624PheLeu: 2.624 ± 0.034	0.42PheMet: 0.42 ± 0.012	0.602PheAsn: 0.602 ± 0.014	1.389PhePro: 1.389 ± 0.024	0.74PheGln: 0.74 ± 0.015	1.841PheArg: 1.841 ± 0.026	1.46PheSer: 1.46 ± 0.023	2.083PheThr: 2.083 ± 0.029	2.256PheVal: 2.256 ± 0.033	0.447PheTrp: 0.447 ± 0.013	0.593PheTyr: 0.593 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	10.369GlyAla: 10.369 ± 0.069	0.835GlyCys: 0.835 ± 0.018	5.168GlyAsp: 5.168 ± 0.043	5.164GlyGlu: 5.164 ± 0.048	2.846GlyPhe: 2.846 ± 0.036	8.452GlyGly: 8.452 ± 0.071	2.327GlyHis: 2.327 ± 0.031	3.58GlyIle: 3.58 ± 0.039	2.527GlyLys: 2.527 ± 0.038	9.216GlyLeu: 9.216 ± 0.064	1.996GlyMet: 1.996 ± 0.028	1.835GlyAsn: 1.835 ± 0.032	4.861GlyPro: 4.861 ± 0.048	2.685GlyGln: 2.685 ± 0.036	7.467GlyArg: 7.467 ± 0.06	5.327GlySer: 5.327 ± 0.056	6.335GlyThr: 6.335 ± 0.06	7.426GlyVal: 7.426 ± 0.059	1.692GlyTrp: 1.692 ± 0.026	2.289GlyTyr: 2.289 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	2.662HisAla: 2.662 ± 0.03	0.216HisCys: 0.216 ± 0.01	1.363HisAsp: 1.363 ± 0.021	1.251HisGlu: 1.251 ± 0.018	0.669HisPhe: 0.669 ± 0.014	2.382HisGly: 2.382 ± 0.029	0.724HisHis: 0.724 ± 0.015	0.721HisIle: 0.721 ± 0.017	0.393HisLys: 0.393 ± 0.012	2.43HisLeu: 2.43 ± 0.031	0.347HisMet: 0.347 ± 0.012	0.395HisAsn: 0.395 ± 0.01	1.783HisPro: 1.783 ± 0.025	0.643HisGln: 0.643 ± 0.014	2.075HisArg: 2.075 ± 0.029	1.062HisSer: 1.062 ± 0.018	1.367HisThr: 1.367 ± 0.019	1.734HisVal: 1.734 ± 0.024	0.376HisTrp: 0.376 ± 0.013	0.499HisTyr: 0.499 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	4.828IleAla: 4.828 ± 0.048	0.29IleCys: 0.29 ± 0.01	2.364IleAsp: 2.364 ± 0.028	2.018IleGlu: 2.018 ± 0.03	0.736IlePhe: 0.736 ± 0.02	3.59IleGly: 3.59 ± 0.041	0.644IleHis: 0.644 ± 0.016	0.914IleIle: 0.914 ± 0.019	0.794IleLys: 0.794 ± 0.021	2.546IleLeu: 2.546 ± 0.034	0.459IleMet: 0.459 ± 0.016	0.733IleAsn: 0.733 ± 0.019	1.889IlePro: 1.889 ± 0.027	0.78IleGln: 0.78 ± 0.017	2.349IleArg: 2.349 ± 0.031	1.742IleSer: 1.742 ± 0.025	2.321IleThr: 2.321 ± 0.03	2.777IleVal: 2.777 ± 0.034	0.386IleTrp: 0.386 ± 0.011	0.55IleTyr: 0.55 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	3.164LysAla: 3.164 ± 0.044	0.125LysCys: 0.125 ± 0.007	1.442LysAsp: 1.442 ± 0.029	1.293LysGlu: 1.293 ± 0.025	0.505LysPhe: 0.505 ± 0.015	1.993LysGly: 1.993 ± 0.03	0.458LysHis: 0.458 ± 0.014	0.879LysIle: 0.879 ± 0.021	0.924LysLys: 0.924 ± 0.027	2.159LysLeu: 2.159 ± 0.032	0.403LysMet: 0.403 ± 0.012	0.593LysAsn: 0.593 ± 0.017	1.461LysPro: 1.461 ± 0.026	0.76LysGln: 0.76 ± 0.019	1.493LysArg: 1.493 ± 0.021	1.275LysSer: 1.275 ± 0.024	1.423LysThr: 1.423 ± 0.025	2.033LysVal: 2.033 ± 0.034	0.321LysTrp: 0.321 ± 0.01	0.503LysTyr: 0.503 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.65LeuAla: 14.65 ± 0.092	0.852LeuCys: 0.852 ± 0.019	6.662LeuAsp: 6.662 ± 0.052	4.847LeuGlu: 4.847 ± 0.044	2.641LeuPhe: 2.641 ± 0.034	9.055LeuGly: 9.055 ± 0.064	2.288LeuHis: 2.288 ± 0.032	3.362LeuIle: 3.362 ± 0.04	2.239LeuLys: 2.239 ± 0.03	11.118LeuLeu: 11.118 ± 0.093	1.7LeuMet: 1.7 ± 0.027	1.776LeuAsn: 1.776 ± 0.026	6.474LeuPro: 6.474 ± 0.056	2.237LeuGln: 2.237 ± 0.026	8.498LeuArg: 8.498 ± 0.067	5.352LeuSer: 5.352 ± 0.047	6.96LeuThr: 6.96 ± 0.054	8.697LeuVal: 8.697 ± 0.068	1.315LeuTrp: 1.315 ± 0.022	1.935LeuTyr: 1.935 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	2.273MetAla: 2.273 ± 0.03	0.135MetCys: 0.135 ± 0.007	0.907MetAsp: 0.907 ± 0.017	0.773MetGlu: 0.773 ± 0.014	0.472MetPhe: 0.472 ± 0.013	1.339MetGly: 1.339 ± 0.026	0.362MetHis: 0.362 ± 0.011	0.668MetIle: 0.668 ± 0.017	0.433MetLys: 0.433 ± 0.012	1.659MetLeu: 1.659 ± 0.025	0.301MetMet: 0.301 ± 0.012	0.455MetAsn: 0.455 ± 0.014	1.147MetPro: 1.147 ± 0.02	0.445MetGln: 0.445 ± 0.012	1.47MetArg: 1.47 ± 0.024	1.339MetSer: 1.339 ± 0.02	1.575MetThr: 1.575 ± 0.024	1.341MetVal: 1.341 ± 0.023	0.23MetTrp: 0.23 ± 0.01	0.338MetTyr: 0.338 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.289AsnAla: 2.289 ± 0.03	0.18AsnCys: 0.18 ± 0.008	1.023AsnAsp: 1.023 ± 0.021	0.881AsnGlu: 0.881 ± 0.017	0.529AsnPhe: 0.529 ± 0.015	1.93AsnGly: 1.93 ± 0.03	0.43AsnHis: 0.43 ± 0.012	0.706AsnIle: 0.706 ± 0.017	0.466AsnLys: 0.466 ± 0.014	1.728AsnLeu: 1.728 ± 0.027	0.297AsnMet: 0.297 ± 0.01	0.482AsnAsn: 0.482 ± 0.015	1.407AsnPro: 1.407 ± 0.025	0.571AsnGln: 0.571 ± 0.016	1.307AsnArg: 1.307 ± 0.022	1.042AsnSer: 1.042 ± 0.022	1.213AsnThr: 1.213 ± 0.025	1.447AsnVal: 1.447 ± 0.027	0.328AsnTrp: 0.328 ± 0.012	0.45AsnTyr: 0.45 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.058ProAla: 8.058 ± 0.058	0.339ProCys: 0.339 ± 0.012	4.543ProAsp: 4.543 ± 0.042	4.409ProGlu: 4.409 ± 0.043	1.536ProPhe: 1.536 ± 0.025	6.212ProGly: 6.212 ± 0.05	1.425ProHis: 1.425 ± 0.021	1.373ProIle: 1.373 ± 0.025	1.316ProLys: 1.316 ± 0.025	5.368ProLeu: 5.368 ± 0.048	0.983ProMet: 0.983 ± 0.019	0.983ProAsn: 0.983 ± 0.019	3.512ProPro: 3.512 ± 0.054	1.799ProGln: 1.799 ± 0.034	3.837ProArg: 3.837 ± 0.042	3.275ProSer: 3.275 ± 0.044	3.447ProThr: 3.447 ± 0.044	5.425ProVal: 5.425 ± 0.049	0.903ProTrp: 0.903 ± 0.017	1.465ProTyr: 1.465 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.793GlnAla: 3.793 ± 0.041	0.176GlnCys: 0.176 ± 0.008	1.477GlnAsp: 1.477 ± 0.024	1.566GlnGlu: 1.566 ± 0.025	0.71GlnPhe: 0.71 ± 0.015	2.313GlnGly: 2.313 ± 0.032	0.672GlnHis: 0.672 ± 0.017	1.117GlnIle: 1.117 ± 0.023	0.664GlnLys: 0.664 ± 0.018	3.117GlnLeu: 3.117 ± 0.036	0.511GlnMet: 0.511 ± 0.013	0.534GlnAsn: 0.534 ± 0.016	1.737GlnPro: 1.737 ± 0.035	1.304GlnGln: 1.304 ± 0.027	2.378GlnArg: 2.378 ± 0.032	1.33GlnSer: 1.33 ± 0.023	1.423GlnThr: 1.423 ± 0.023	2.411GlnVal: 2.411 ± 0.033	0.511GlnTrp: 0.511 ± 0.014	0.662GlnTyr: 0.662 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.438ArgAla: 9.438 ± 0.07	0.588ArgCys: 0.588 ± 0.014	4.214ArgAsp: 4.214 ± 0.043	4.824ArgGlu: 4.824 ± 0.046	2.306ArgPhe: 2.306 ± 0.032	5.638ArgGly: 5.638 ± 0.052	2.116ArgHis: 2.116 ± 0.028	3.276ArgIle: 3.276 ± 0.034	1.722ArgLys: 1.722 ± 0.025	8.799ArgLeu: 8.799 ± 0.071	1.738ArgMet: 1.738 ± 0.023	1.359ArgAsn: 1.359 ± 0.022	4.878ArgPro: 4.878 ± 0.046	2.364ArgGln: 2.364 ± 0.032	7.539ArgArg: 7.539 ± 0.065	3.938ArgSer: 3.938 ± 0.037	5.327ArgThr: 5.327 ± 0.049	5.767ArgVal: 5.767 ± 0.047	1.376ArgTrp: 1.376 ± 0.022	1.82ArgTyr: 1.82 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.664SerAla: 6.664 ± 0.059	0.417SerCys: 0.417 ± 0.012	2.792SerAsp: 2.792 ± 0.034	2.554SerGlu: 2.554 ± 0.036	1.525SerPhe: 1.525 ± 0.024	5.975SerGly: 5.975 ± 0.055	1.018SerHis: 1.018 ± 0.019	1.502SerIle: 1.502 ± 0.027	1.144SerLys: 1.144 ± 0.022	4.883SerLeu: 4.883 ± 0.039	1.108SerMet: 1.108 ± 0.023	0.953SerAsn: 0.953 ± 0.018	3.222SerPro: 3.222 ± 0.04	1.29SerGln: 1.29 ± 0.024	3.669SerArg: 3.669 ± 0.038	3.002SerSer: 3.002 ± 0.05	3.213SerThr: 3.213 ± 0.044	4.367SerVal: 4.367 ± 0.041	0.918SerTrp: 0.918 ± 0.021	1.313SerTyr: 1.313 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	8.852ThrAla: 8.852 ± 0.067	0.459ThrCys: 0.459 ± 0.012	3.864ThrAsp: 3.864 ± 0.041	3.345ThrGlu: 3.345 ± 0.034	1.697ThrPhe: 1.697 ± 0.027	6.618ThrGly: 6.618 ± 0.06	1.263ThrHis: 1.263 ± 0.022	1.803ThrIle: 1.803 ± 0.027	1.328ThrLys: 1.328 ± 0.029	5.733ThrLeu: 5.733 ± 0.046	0.968ThrMet: 0.968 ± 0.02	1.115ThrAsn: 1.115 ± 0.024	4.27ThrPro: 4.27 ± 0.045	1.439ThrGln: 1.439 ± 0.022	3.931ThrArg: 3.931 ± 0.037	3.351ThrSer: 3.351 ± 0.04	4.113ThrThr: 4.113 ± 0.06	6.125ThrVal: 6.125 ± 0.052	0.98ThrTrp: 0.98 ± 0.022	1.512ThrTyr: 1.512 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.405ValAla: 10.405 ± 0.069	0.762ValCys: 0.762 ± 0.021	4.98ValAsp: 4.98 ± 0.046	4.79ValGlu: 4.79 ± 0.051	2.414ValPhe: 2.414 ± 0.025	6.526ValGly: 6.526 ± 0.051	1.928ValHis: 1.928 ± 0.028	2.974ValIle: 2.974 ± 0.036	1.844ValLys: 1.844 ± 0.031	9.332ValLeu: 9.332 ± 0.071	1.455ValMet: 1.455 ± 0.024	1.759ValAsn: 1.759 ± 0.026	5.156ValPro: 5.156 ± 0.046	2.116ValGln: 2.116 ± 0.031	7.126ValArg: 7.126 ± 0.056	4.418ValSer: 4.418 ± 0.038	5.697ValThr: 5.697 ± 0.047	7.944ValVal: 7.944 ± 0.071	1.183ValTrp: 1.183 ± 0.022	1.624ValTyr: 1.624 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.658TrpAla: 1.658 ± 0.026	0.146TrpCys: 0.146 ± 0.007	0.878TrpAsp: 0.878 ± 0.018	0.78TrpGlu: 0.78 ± 0.017	0.519TrpPhe: 0.519 ± 0.015	1.11TrpGly: 1.11 ± 0.022	0.383TrpHis: 0.383 ± 0.014	0.579TrpIle: 0.579 ± 0.015	0.402TrpLys: 0.402 ± 0.012	1.797TrpLeu: 1.797 ± 0.031	0.302TrpMet: 0.302 ± 0.011	0.445TrpAsn: 0.445 ± 0.012	0.796TrpPro: 0.796 ± 0.016	0.661TrpGln: 0.661 ± 0.016	1.36TrpArg: 1.36 ± 0.021	0.974TrpSer: 0.974 ± 0.022	1.093TrpThr: 1.093 ± 0.021	0.987TrpVal: 0.987 ± 0.018	0.342TrpTrp: 0.342 ± 0.011	0.382TrpTyr: 0.382 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.776TyrAla: 2.776 ± 0.033	0.187TyrCys: 0.187 ± 0.008	1.68TyrAsp: 1.68 ± 0.03	1.366TyrGlu: 1.366 ± 0.024	0.675TyrPhe: 0.675 ± 0.017	2.386TyrGly: 2.386 ± 0.03	0.402TyrHis: 0.402 ± 0.014	0.544TyrIle: 0.544 ± 0.016	0.44TyrLys: 0.44 ± 0.015	2.113TyrLeu: 2.113 ± 0.031	0.28TyrMet: 0.28 ± 0.01	0.461TyrAsn: 0.461 ± 0.014	1.074TyrPro: 1.074 ± 0.021	0.646TyrGln: 0.646 ± 0.015	1.825TyrArg: 1.825 ± 0.026	1.02TyrSer: 1.02 ± 0.019	1.276TyrThr: 1.276 ± 0.026	1.764TyrVal: 1.764 ± 0.024	0.383TyrTrp: 0.383 ± 0.012	0.501TyrTyr: 0.501 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8803 proteins (2861888 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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