Thermus phage phiOH16

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S7J239|A0A1S7J239_9VIRU Minor coat protein OS=Thermus phage phiOH16 OX=1968340 GN=VI PE=4 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84LALPILLVLLSLGWGGPAFAQEE25 pKa = 4.27HH26 pKa = 5.76FKK28 pKa = 11.17GAIRR32 pKa = 11.84STVQGMTLDD41 pKa = 5.61EE42 pKa = 4.86IIQWSDD48 pKa = 2.68AQFRR52 pKa = 11.84RR53 pKa = 11.84QATIGRR59 pKa = 11.84YY60 pKa = 7.9VRR62 pKa = 11.84WGGAIVLGSALVYY75 pKa = 9.11TALDD79 pKa = 3.25YY80 pKa = 10.78FYY82 pKa = 11.29NVLKK86 pKa = 10.73AQTGTSLDD94 pKa = 2.93RR95 pKa = 11.84WYY97 pKa = 10.45FWSNPYY103 pKa = 10.18VEE105 pKa = 4.75VGRR108 pKa = 11.84CIDD111 pKa = 3.37RR112 pKa = 11.84GSYY115 pKa = 10.46RR116 pKa = 11.84DD117 pKa = 4.79FIAVAYY123 pKa = 10.47VNGRR127 pKa = 11.84QVNYY131 pKa = 10.16YY132 pKa = 8.52ATSHH136 pKa = 6.64FGKK139 pKa = 10.31CDD141 pKa = 3.49VPSLQSFTEE150 pKa = 4.08YY151 pKa = 9.95WYY153 pKa = 10.96GLHH156 pKa = 7.07LVRR159 pKa = 11.84NYY161 pKa = 11.07SEE163 pKa = 4.26LQGVPTQYY171 pKa = 10.77VSPPPGTCPSGWTCIAVAPQLDD193 pKa = 4.49RR194 pKa = 11.84PPLPDD199 pKa = 3.39WLQSHH204 pKa = 7.6PDD206 pKa = 3.16AADD209 pKa = 3.53GVKK212 pKa = 10.1QAVTTYY218 pKa = 11.07LDD220 pKa = 3.8RR221 pKa = 11.84NPIGSPYY228 pKa = 10.58APYY231 pKa = 10.19PGVQLEE237 pKa = 4.97PIPNPNQWTDD247 pKa = 3.29NPFTRR252 pKa = 11.84PDD254 pKa = 3.46IDD256 pKa = 3.52TDD258 pKa = 3.77GDD260 pKa = 3.69GWPDD264 pKa = 3.31PVEE267 pKa = 3.51WRR269 pKa = 11.84EE270 pKa = 3.72ANRR273 pKa = 11.84RR274 pKa = 11.84GVPWPDD280 pKa = 5.02LINNPQAYY288 pKa = 7.88PDD290 pKa = 4.32PNGDD294 pKa = 3.58PDD296 pKa = 4.77GDD298 pKa = 3.67GFTNLEE304 pKa = 4.12EE305 pKa = 4.42VQQGTDD311 pKa = 3.17PYY313 pKa = 10.74DD314 pKa = 3.9PTSHH318 pKa = 7.06PVRR321 pKa = 11.84RR322 pKa = 11.84SPTSPWVDD330 pKa = 3.05TDD332 pKa = 3.34GDD334 pKa = 4.29GYY336 pKa = 11.18SDD338 pKa = 3.59EE339 pKa = 4.36EE340 pKa = 4.49EE341 pKa = 3.98IRR343 pKa = 11.84KK344 pKa = 7.8GTNPNDD350 pKa = 3.54PASYY354 pKa = 10.12PDD356 pKa = 4.25TPPEE360 pKa = 3.93QQPEE364 pKa = 4.26EE365 pKa = 4.51NPDD368 pKa = 3.22EE369 pKa = 4.5PQWPGGPPSGRR380 pKa = 11.84IDD382 pKa = 3.4PVQLPEE388 pKa = 3.98VEE390 pKa = 4.04QVEE393 pKa = 4.5RR394 pKa = 11.84EE395 pKa = 4.33KK396 pKa = 11.31LPEE399 pKa = 3.83WDD401 pKa = 3.96KK402 pKa = 11.71LNPLAEE408 pKa = 4.1AWRR411 pKa = 11.84QQVVDD416 pKa = 3.9RR417 pKa = 11.84VSQKK421 pKa = 10.05FAEE424 pKa = 4.35VQNILKK430 pKa = 10.46DD431 pKa = 3.55RR432 pKa = 11.84FPFGIIAAIRR442 pKa = 11.84QRR444 pKa = 11.84VSFADD449 pKa = 3.68AQCAIQLSLPPLGTLAVDD467 pKa = 3.84ICSTPVWQLATSFRR481 pKa = 11.84PVLAGLVWVAFGFAVIRR498 pKa = 11.84RR499 pKa = 11.84ALDD502 pKa = 3.3VQKK505 pKa = 11.3

Molecular weight: 56.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S7J233|A0A1S7J233_9VIRU Replication endonuclease OS=Thermus phage phiOH16 OX=1968340 GN=II PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 4.46FVGVDD7 pKa = 3.69VSAKK11 pKa = 10.25RR12 pKa = 11.84LDD14 pKa = 3.47VALGGKK20 pKa = 9.25LLSFPNPEE28 pKa = 4.87GIPDD32 pKa = 5.34LIAALPPGAVVGLEE46 pKa = 3.73ATGVYY51 pKa = 9.63GRR53 pKa = 11.84PLAFALHH60 pKa = 6.37RR61 pKa = 11.84AGFHH65 pKa = 6.18VYY67 pKa = 9.79VLNPFAVKK75 pKa = 10.18SYY77 pKa = 10.94ARR79 pKa = 11.84SLLLRR84 pKa = 11.84SKK86 pKa = 9.48TDD88 pKa = 3.15RR89 pKa = 11.84VDD91 pKa = 2.92ARR93 pKa = 11.84LIARR97 pKa = 11.84YY98 pKa = 8.75LADD101 pKa = 5.26RR102 pKa = 11.84YY103 pKa = 10.2PDD105 pKa = 3.52LPLYY109 pKa = 10.24EE110 pKa = 4.63PAPEE114 pKa = 4.28VIYY117 pKa = 10.57QAGLLVRR124 pKa = 11.84FARR127 pKa = 11.84GLVVQRR133 pKa = 11.84VAVLNRR139 pKa = 11.84LHH141 pKa = 6.74AWQYY145 pKa = 10.6AWPQGLEE152 pKa = 4.35VVAHH156 pKa = 5.65VPRR159 pKa = 11.84TLEE162 pKa = 3.76ALRR165 pKa = 11.84GNLEE169 pKa = 3.95ALAVDD174 pKa = 5.05LLRR177 pKa = 11.84SDD179 pKa = 3.9PEE181 pKa = 3.29AWRR184 pKa = 11.84QFRR187 pKa = 11.84ALQTLPGIGPSSALTILAYY206 pKa = 10.7SGDD209 pKa = 3.32LRR211 pKa = 11.84RR212 pKa = 11.84FKK214 pKa = 10.7SARR217 pKa = 11.84AYY219 pKa = 10.54AAFTGLAPVVHH230 pKa = 6.12QSGALPEE237 pKa = 3.98VGRR240 pKa = 11.84ISRR243 pKa = 11.84AGPAPLRR250 pKa = 11.84ATFWLASLHH259 pKa = 5.32AVKK262 pKa = 10.69VEE264 pKa = 4.65PYY266 pKa = 9.19ASLYY270 pKa = 10.91ARR272 pKa = 11.84LLQAGKK278 pKa = 8.58PPQVARR284 pKa = 11.84VAVAHH289 pKa = 6.35RR290 pKa = 11.84LARR293 pKa = 11.84AAWAVVVRR301 pKa = 11.84SVV303 pKa = 3.24

Molecular weight: 32.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1921

30

505

213.4

23.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.984 ± 1.497

0.469 ± 0.262

5.362 ± 0.817

4.945 ± 0.742

3.852 ± 0.288

7.86 ± 0.493

1.51 ± 0.251

3.644 ± 0.804

2.811 ± 0.455

10.828 ± 1.499

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.406 ± 0.411

2.134 ± 0.507

7.236 ± 1.129

3.54 ± 0.853

7.496 ± 0.791

5.414 ± 0.364

3.8 ± 0.526

9.682 ± 0.856

3.227 ± 0.446

3.8 ± 0.332
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski