Amino acid dipepetide frequency for Mycobacterium phage Nilo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.982AlaAla: 10.982 ± 1.184
0.706AlaCys: 0.706 ± 0.159
5.204AlaAsp: 5.204 ± 0.503
5.469AlaGlu: 5.469 ± 0.56
3.44AlaPhe: 3.44 ± 0.473
8.203AlaGly: 8.203 ± 0.81
1.323AlaHis: 1.323 ± 0.31
4.058AlaIle: 4.058 ± 0.5
6.086AlaLys: 6.086 ± 0.69
8.203AlaLeu: 8.203 ± 0.599
2.249AlaMet: 2.249 ± 0.29
3.528AlaAsn: 3.528 ± 0.508
3.837AlaPro: 3.837 ± 0.431
4.19AlaGln: 4.19 ± 0.515
5.557AlaArg: 5.557 ± 0.578
4.675AlaSer: 4.675 ± 0.535
6.307AlaThr: 6.307 ± 0.522
5.381AlaVal: 5.381 ± 0.45
1.367AlaTrp: 1.367 ± 0.279
2.734AlaTyr: 2.734 ± 0.337
0.0AlaXaa: 0.0 ± 0.0
Cys
0.617CysAla: 0.617 ± 0.19
0.088CysCys: 0.088 ± 0.057
0.441CysAsp: 0.441 ± 0.14
0.838CysGlu: 0.838 ± 0.217
0.397CysPhe: 0.397 ± 0.142
1.014CysGly: 1.014 ± 0.29
0.221CysHis: 0.221 ± 0.111
0.485CysIle: 0.485 ± 0.16
0.441CysLys: 0.441 ± 0.138
0.662CysLeu: 0.662 ± 0.188
0.132CysMet: 0.132 ± 0.083
0.441CysAsn: 0.441 ± 0.127
0.662CysPro: 0.662 ± 0.164
0.353CysGln: 0.353 ± 0.125
0.617CysArg: 0.617 ± 0.179
0.441CysSer: 0.441 ± 0.127
0.529CysThr: 0.529 ± 0.175
0.397CysVal: 0.397 ± 0.147
0.176CysTrp: 0.176 ± 0.081
0.353CysTyr: 0.353 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
4.631AspAla: 4.631 ± 0.689
0.838AspCys: 0.838 ± 0.253
5.116AspAsp: 5.116 ± 0.97
4.19AspGlu: 4.19 ± 0.581
2.558AspPhe: 2.558 ± 0.358
5.469AspGly: 5.469 ± 0.481
1.191AspHis: 1.191 ± 0.26
3.969AspIle: 3.969 ± 0.395
2.911AspLys: 2.911 ± 0.414
5.337AspLeu: 5.337 ± 0.372
1.411AspMet: 1.411 ± 0.212
2.382AspAsn: 2.382 ± 0.349
4.587AspPro: 4.587 ± 0.441
2.205AspGln: 2.205 ± 0.304
3.308AspArg: 3.308 ± 0.324
3.22AspSer: 3.22 ± 0.441
3.528AspThr: 3.528 ± 0.384
4.278AspVal: 4.278 ± 0.463
1.279AspTrp: 1.279 ± 0.231
1.985AspTyr: 1.985 ± 0.298
0.0AspXaa: 0.0 ± 0.0
Glu
6.263GluAla: 6.263 ± 0.441
0.485GluCys: 0.485 ± 0.152
4.675GluAsp: 4.675 ± 0.691
3.969GluGlu: 3.969 ± 0.344
1.985GluPhe: 1.985 ± 0.313
4.851GluGly: 4.851 ± 0.409
1.764GluHis: 1.764 ± 0.311
2.734GluIle: 2.734 ± 0.427
3.087GluLys: 3.087 ± 0.411
5.645GluLeu: 5.645 ± 0.446
1.588GluMet: 1.588 ± 0.246
1.764GluAsn: 1.764 ± 0.282
3.396GluPro: 3.396 ± 0.453
2.558GluGln: 2.558 ± 0.348
4.102GluArg: 4.102 ± 0.461
2.867GluSer: 2.867 ± 0.278
4.058GluThr: 4.058 ± 0.46
4.322GluVal: 4.322 ± 0.472
1.235GluTrp: 1.235 ± 0.232
2.293GluTyr: 2.293 ± 0.425
0.0GluXaa: 0.0 ± 0.0
Phe
3.484PheAla: 3.484 ± 0.385
0.353PheCys: 0.353 ± 0.124
2.161PheAsp: 2.161 ± 0.32
2.293PheGlu: 2.293 ± 0.326
1.147PhePhe: 1.147 ± 0.215
3.264PheGly: 3.264 ± 0.463
0.882PheHis: 0.882 ± 0.215
1.191PheIle: 1.191 ± 0.331
1.764PheLys: 1.764 ± 0.278
2.293PheLeu: 2.293 ± 0.318
0.617PheMet: 0.617 ± 0.203
1.411PheAsn: 1.411 ± 0.255
1.103PhePro: 1.103 ± 0.195
1.588PheGln: 1.588 ± 0.191
2.249PheArg: 2.249 ± 0.262
1.764PheSer: 1.764 ± 0.304
1.941PheThr: 1.941 ± 0.257
2.117PheVal: 2.117 ± 0.286
0.353PheTrp: 0.353 ± 0.106
0.706PheTyr: 0.706 ± 0.162
0.0PheXaa: 0.0 ± 0.0
Gly
5.248GlyAla: 5.248 ± 0.671
0.662GlyCys: 0.662 ± 0.156
5.072GlyAsp: 5.072 ± 0.475
4.278GlyGlu: 4.278 ± 0.459
2.117GlyPhe: 2.117 ± 0.311
7.409GlyGly: 7.409 ± 1.23
1.896GlyHis: 1.896 ± 0.306
4.763GlyIle: 4.763 ± 0.559
4.984GlyLys: 4.984 ± 0.631
5.954GlyLeu: 5.954 ± 0.577
2.161GlyMet: 2.161 ± 0.335
4.366GlyAsn: 4.366 ± 0.531
3.661GlyPro: 3.661 ± 0.419
2.337GlyGln: 2.337 ± 0.269
4.41GlyArg: 4.41 ± 0.375
5.028GlySer: 5.028 ± 0.704
6.704GlyThr: 6.704 ± 0.812
5.601GlyVal: 5.601 ± 0.605
2.205GlyTrp: 2.205 ± 0.289
3.131GlyTyr: 3.131 ± 0.354
0.0GlyXaa: 0.0 ± 0.0
His
1.235HisAla: 1.235 ± 0.274
0.176HisCys: 0.176 ± 0.086
1.279HisAsp: 1.279 ± 0.288
1.808HisGlu: 1.808 ± 0.3
0.397HisPhe: 0.397 ± 0.134
1.544HisGly: 1.544 ± 0.295
0.485HisHis: 0.485 ± 0.155
0.882HisIle: 0.882 ± 0.181
1.147HisLys: 1.147 ± 0.252
2.073HisLeu: 2.073 ± 0.299
0.75HisMet: 0.75 ± 0.16
0.485HisAsn: 0.485 ± 0.166
1.764HisPro: 1.764 ± 0.24
0.353HisGln: 0.353 ± 0.116
1.147HisArg: 1.147 ± 0.208
0.926HisSer: 0.926 ± 0.251
1.323HisThr: 1.323 ± 0.292
1.411HisVal: 1.411 ± 0.262
0.309HisTrp: 0.309 ± 0.108
0.882HisTyr: 0.882 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
4.587IleAla: 4.587 ± 0.539
0.353IleCys: 0.353 ± 0.136
3.616IleAsp: 3.616 ± 0.369
3.616IleGlu: 3.616 ± 0.378
1.058IlePhe: 1.058 ± 0.197
3.308IleGly: 3.308 ± 0.416
1.323IleHis: 1.323 ± 0.254
2.426IleIle: 2.426 ± 0.294
1.808IleLys: 1.808 ± 0.263
3.44IleLeu: 3.44 ± 0.399
0.926IleMet: 0.926 ± 0.195
1.411IleAsn: 1.411 ± 0.294
2.602IlePro: 2.602 ± 0.313
2.382IleGln: 2.382 ± 0.312
2.999IleArg: 2.999 ± 0.363
2.646IleSer: 2.646 ± 0.379
2.999IleThr: 2.999 ± 0.352
3.087IleVal: 3.087 ± 0.425
1.323IleTrp: 1.323 ± 0.178
0.926IleTyr: 0.926 ± 0.197
0.0IleXaa: 0.0 ± 0.0
Lys
6.616LysAla: 6.616 ± 0.829
0.397LysCys: 0.397 ± 0.122
2.646LysAsp: 2.646 ± 0.428
3.087LysGlu: 3.087 ± 0.432
2.205LysPhe: 2.205 ± 0.288
4.102LysGly: 4.102 ± 0.412
0.97LysHis: 0.97 ± 0.216
2.249LysIle: 2.249 ± 0.291
3.308LysLys: 3.308 ± 0.608
4.675LysLeu: 4.675 ± 0.476
1.235LysMet: 1.235 ± 0.25
1.896LysAsn: 1.896 ± 0.238
3.969LysPro: 3.969 ± 0.576
1.941LysGln: 1.941 ± 0.313
3.793LysArg: 3.793 ± 0.528
2.249LysSer: 2.249 ± 0.358
2.911LysThr: 2.911 ± 0.337
3.837LysVal: 3.837 ± 0.48
0.706LysTrp: 0.706 ± 0.206
1.5LysTyr: 1.5 ± 0.266
0.0LysXaa: 0.0 ± 0.0
Leu
7.542LeuAla: 7.542 ± 0.456
0.706LeuCys: 0.706 ± 0.193
5.292LeuAsp: 5.292 ± 0.579
6.13LeuGlu: 6.13 ± 0.484
2.205LeuPhe: 2.205 ± 0.233
6.704LeuGly: 6.704 ± 0.703
1.411LeuHis: 1.411 ± 0.238
3.22LeuIle: 3.22 ± 0.362
4.146LeuLys: 4.146 ± 0.472
6.748LeuLeu: 6.748 ± 0.606
1.191LeuMet: 1.191 ± 0.239
3.352LeuAsn: 3.352 ± 0.426
4.234LeuPro: 4.234 ± 0.442
3.528LeuGln: 3.528 ± 0.393
6.307LeuArg: 6.307 ± 0.659
5.16LeuSer: 5.16 ± 0.558
4.454LeuThr: 4.454 ± 0.503
5.16LeuVal: 5.16 ± 0.474
1.147LeuTrp: 1.147 ± 0.231
1.72LeuTyr: 1.72 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
2.426MetAla: 2.426 ± 0.36
0.309MetCys: 0.309 ± 0.105
1.588MetAsp: 1.588 ± 0.251
1.191MetGlu: 1.191 ± 0.258
0.882MetPhe: 0.882 ± 0.186
1.411MetGly: 1.411 ± 0.184
0.353MetHis: 0.353 ± 0.099
0.926MetIle: 0.926 ± 0.214
0.926MetLys: 0.926 ± 0.197
1.764MetLeu: 1.764 ± 0.218
0.485MetMet: 0.485 ± 0.121
0.75MetAsn: 0.75 ± 0.15
1.367MetPro: 1.367 ± 0.247
0.926MetGln: 0.926 ± 0.206
1.367MetArg: 1.367 ± 0.297
1.323MetSer: 1.323 ± 0.239
2.029MetThr: 2.029 ± 0.279
1.72MetVal: 1.72 ± 0.249
0.176MetTrp: 0.176 ± 0.079
0.441MetTyr: 0.441 ± 0.145
0.0MetXaa: 0.0 ± 0.0
Asn
4.41AsnAla: 4.41 ± 0.385
0.309AsnCys: 0.309 ± 0.12
2.029AsnAsp: 2.029 ± 0.31
1.896AsnGlu: 1.896 ± 0.283
1.191AsnPhe: 1.191 ± 0.257
3.352AsnGly: 3.352 ± 0.34
0.441AsnHis: 0.441 ± 0.128
1.808AsnIle: 1.808 ± 0.281
2.029AsnLys: 2.029 ± 0.343
2.999AsnLeu: 2.999 ± 0.37
0.926AsnMet: 0.926 ± 0.179
1.676AsnAsn: 1.676 ± 0.276
2.955AsnPro: 2.955 ± 0.311
1.632AsnGln: 1.632 ± 0.213
2.249AsnArg: 2.249 ± 0.294
2.205AsnSer: 2.205 ± 0.296
2.602AsnThr: 2.602 ± 0.467
3.352AsnVal: 3.352 ± 0.295
1.058AsnTrp: 1.058 ± 0.241
1.279AsnTyr: 1.279 ± 0.22
0.0AsnXaa: 0.0 ± 0.0
Pro
3.969ProAla: 3.969 ± 0.379
0.706ProCys: 0.706 ± 0.223
3.837ProAsp: 3.837 ± 0.484
4.278ProGlu: 4.278 ± 0.472
1.941ProPhe: 1.941 ± 0.29
4.102ProGly: 4.102 ± 0.457
1.014ProHis: 1.014 ± 0.246
2.337ProIle: 2.337 ± 0.381
3.22ProLys: 3.22 ± 0.397
4.19ProLeu: 4.19 ± 0.378
1.544ProMet: 1.544 ± 0.288
2.426ProAsn: 2.426 ± 0.313
2.602ProPro: 2.602 ± 0.386
1.411ProGln: 1.411 ± 0.227
2.734ProArg: 2.734 ± 0.372
3.705ProSer: 3.705 ± 0.477
3.22ProThr: 3.22 ± 0.381
3.44ProVal: 3.44 ± 0.412
0.926ProTrp: 0.926 ± 0.19
1.5ProTyr: 1.5 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
5.028GlnAla: 5.028 ± 0.661
0.662GlnCys: 0.662 ± 0.188
1.941GlnAsp: 1.941 ± 0.354
2.514GlnGlu: 2.514 ± 0.349
1.455GlnPhe: 1.455 ± 0.283
2.999GlnGly: 2.999 ± 0.567
0.529GlnHis: 0.529 ± 0.137
1.896GlnIle: 1.896 ± 0.274
1.544GlnLys: 1.544 ± 0.283
3.22GlnLeu: 3.22 ± 0.335
1.191GlnMet: 1.191 ± 0.221
1.544GlnAsn: 1.544 ± 0.339
1.764GlnPro: 1.764 ± 0.314
1.588GlnGln: 1.588 ± 0.256
3.264GlnArg: 3.264 ± 0.377
1.985GlnSer: 1.985 ± 0.301
1.764GlnThr: 1.764 ± 0.274
2.646GlnVal: 2.646 ± 0.379
0.706GlnTrp: 0.706 ± 0.215
1.279GlnTyr: 1.279 ± 0.225
0.0GlnXaa: 0.0 ± 0.0
Arg
5.513ArgAla: 5.513 ± 0.563
0.75ArgCys: 0.75 ± 0.192
3.793ArgAsp: 3.793 ± 0.334
3.837ArgGlu: 3.837 ± 0.442
2.337ArgPhe: 2.337 ± 0.366
3.881ArgGly: 3.881 ± 0.357
1.588ArgHis: 1.588 ± 0.314
3.44ArgIle: 3.44 ± 0.535
4.719ArgLys: 4.719 ± 0.567
5.072ArgLeu: 5.072 ± 0.424
1.367ArgMet: 1.367 ± 0.234
3.264ArgAsn: 3.264 ± 0.375
2.69ArgPro: 2.69 ± 0.432
2.602ArgGln: 2.602 ± 0.3
4.675ArgArg: 4.675 ± 0.596
2.779ArgSer: 2.779 ± 0.373
2.337ArgThr: 2.337 ± 0.353
4.146ArgVal: 4.146 ± 0.4
1.632ArgTrp: 1.632 ± 0.448
2.029ArgTyr: 2.029 ± 0.262
0.0ArgXaa: 0.0 ± 0.0
Ser
5.204SerAla: 5.204 ± 0.558
0.397SerCys: 0.397 ± 0.135
3.705SerAsp: 3.705 ± 0.436
3.396SerGlu: 3.396 ± 0.317
1.455SerPhe: 1.455 ± 0.256
5.292SerGly: 5.292 ± 0.482
0.926SerHis: 0.926 ± 0.181
2.337SerIle: 2.337 ± 0.288
2.734SerLys: 2.734 ± 0.461
4.587SerLeu: 4.587 ± 0.475
1.058SerMet: 1.058 ± 0.198
2.734SerAsn: 2.734 ± 0.37
1.941SerPro: 1.941 ± 0.251
2.205SerGln: 2.205 ± 0.392
2.646SerArg: 2.646 ± 0.36
3.661SerSer: 3.661 ± 0.405
3.881SerThr: 3.881 ± 0.471
3.44SerVal: 3.44 ± 0.354
1.544SerTrp: 1.544 ± 0.253
2.161SerTyr: 2.161 ± 0.329
0.0SerXaa: 0.0 ± 0.0
Thr
5.425ThrAla: 5.425 ± 0.605
0.397ThrCys: 0.397 ± 0.154
3.572ThrAsp: 3.572 ± 0.468
2.734ThrGlu: 2.734 ± 0.381
2.293ThrPhe: 2.293 ± 0.337
6.219ThrGly: 6.219 ± 0.655
1.147ThrHis: 1.147 ± 0.218
3.264ThrIle: 3.264 ± 0.308
3.131ThrLys: 3.131 ± 0.378
5.248ThrLeu: 5.248 ± 0.403
0.97ThrMet: 0.97 ± 0.223
2.382ThrAsn: 2.382 ± 0.314
3.705ThrPro: 3.705 ± 0.501
3.131ThrGln: 3.131 ± 0.335
3.661ThrArg: 3.661 ± 0.456
3.44ThrSer: 3.44 ± 0.442
4.013ThrThr: 4.013 ± 0.449
4.631ThrVal: 4.631 ± 0.492
1.147ThrTrp: 1.147 ± 0.214
1.455ThrTyr: 1.455 ± 0.233
0.0ThrXaa: 0.0 ± 0.0
Val
6.174ValAla: 6.174 ± 0.462
0.529ValCys: 0.529 ± 0.19
4.807ValAsp: 4.807 ± 0.442
4.895ValGlu: 4.895 ± 0.422
1.985ValPhe: 1.985 ± 0.285
4.719ValGly: 4.719 ± 0.45
1.676ValHis: 1.676 ± 0.284
2.646ValIle: 2.646 ± 0.366
3.44ValLys: 3.44 ± 0.453
5.028ValLeu: 5.028 ± 0.517
1.5ValMet: 1.5 ± 0.242
2.734ValAsn: 2.734 ± 0.274
3.793ValPro: 3.793 ± 0.442
2.69ValGln: 2.69 ± 0.379
4.278ValArg: 4.278 ± 0.343
4.013ValSer: 4.013 ± 0.405
4.851ValThr: 4.851 ± 0.464
5.028ValVal: 5.028 ± 0.574
0.97ValTrp: 0.97 ± 0.253
2.337ValTyr: 2.337 ± 0.336
0.0ValXaa: 0.0 ± 0.0
Trp
1.72TrpAla: 1.72 ± 0.257
0.309TrpCys: 0.309 ± 0.126
1.279TrpAsp: 1.279 ± 0.283
1.279TrpGlu: 1.279 ± 0.22
0.353TrpPhe: 0.353 ± 0.108
1.455TrpGly: 1.455 ± 0.212
0.662TrpHis: 0.662 ± 0.154
0.706TrpIle: 0.706 ± 0.171
1.058TrpLys: 1.058 ± 0.213
1.5TrpLeu: 1.5 ± 0.291
0.397TrpMet: 0.397 ± 0.131
0.617TrpAsn: 0.617 ± 0.138
0.706TrpPro: 0.706 ± 0.169
0.706TrpGln: 0.706 ± 0.125
0.97TrpArg: 0.97 ± 0.172
1.367TrpSer: 1.367 ± 0.247
1.455TrpThr: 1.455 ± 0.225
1.455TrpVal: 1.455 ± 0.338
0.485TrpTrp: 0.485 ± 0.179
0.706TrpTyr: 0.706 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.426TyrAla: 2.426 ± 0.379
0.176TyrCys: 0.176 ± 0.108
2.337TyrAsp: 2.337 ± 0.317
1.941TyrGlu: 1.941 ± 0.316
1.279TyrPhe: 1.279 ± 0.204
2.69TyrGly: 2.69 ± 0.352
0.573TyrHis: 0.573 ± 0.176
1.5TyrIle: 1.5 ± 0.218
1.808TyrLys: 1.808 ± 0.278
1.72TyrLeu: 1.72 ± 0.24
0.617TyrMet: 0.617 ± 0.155
1.235TyrAsn: 1.235 ± 0.251
1.764TyrPro: 1.764 ± 0.338
1.191TyrGln: 1.191 ± 0.225
2.117TyrArg: 2.117 ± 0.318
1.896TyrSer: 1.896 ± 0.271
1.058TyrThr: 1.058 ± 0.21
2.646TyrVal: 2.646 ± 0.363
0.441TyrTrp: 0.441 ± 0.168
0.838TyrTyr: 0.838 ± 0.207
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (22675 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski