Trifolium subterraneum (Subterranean clover)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina; IRL clade;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B5Z8I6|A0A1B5Z8I6_TRISU Uncharacterized protein (Fragment) OS=Trifolium subterraneum OX=3900 GN=TSUD_423450 PE=3 SV=1
MM1 pKa = 7.0EE2 pKa = 4.35QLVNRR7 pKa = 11.84LDD9 pKa = 3.41VAMFNAILRR18 pKa = 11.84EE19 pKa = 4.32SADD22 pKa = 3.51EE23 pKa = 4.06MPTYY27 pKa = 10.2PVVNNFVPDD36 pKa = 3.75EE37 pKa = 4.18FSPGPVPNAVYY48 pKa = 9.79EE49 pKa = 4.13ALNNEE54 pKa = 4.86DD55 pKa = 3.94IEE57 pKa = 5.29DD58 pKa = 4.27DD59 pKa = 3.94EE60 pKa = 5.18GCITSFPCTAGSTFYY75 pKa = 10.93APPPASSVVSMLKK88 pKa = 10.38EE89 pKa = 3.83VGTPLLRR96 pKa = 11.84SGSFVLKK103 pKa = 10.52KK104 pKa = 10.59LYY106 pKa = 10.46TSDD109 pKa = 5.68DD110 pKa = 3.97EE111 pKa = 5.34LDD113 pKa = 3.62EE114 pKa = 4.4

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B5Z7T2|A0A1B5Z7T2_TRISU Uncharacterized protein (Fragment) OS=Trifolium subterraneum OX=3900 GN=TSUD_423870 PE=4 SV=1
MM1 pKa = 7.61APQVPLKK8 pKa = 10.93VSILAWRR15 pKa = 11.84LLRR18 pKa = 11.84DD19 pKa = 3.74RR20 pKa = 11.84LPTKK24 pKa = 10.7SNLITRR30 pKa = 11.84GVLPVAAHH38 pKa = 6.22FCVSGCGEE46 pKa = 4.23AEE48 pKa = 4.31SAHH51 pKa = 6.71HH52 pKa = 6.88LFLSCSTFGSLWPLVGSWVGSSLVTTQTLPDD83 pKa = 3.88HH84 pKa = 6.84FVQFTSSAGGSRR96 pKa = 11.84SHH98 pKa = 7.18RR99 pKa = 11.84SFMQLLWLVSVWVVWTEE116 pKa = 3.7RR117 pKa = 11.84NHH119 pKa = 6.12RR120 pKa = 11.84LFRR123 pKa = 11.84GSANSLSHH131 pKa = 6.53MLDD134 pKa = 3.31KK135 pKa = 10.89IKK137 pKa = 9.99TFSYY141 pKa = 9.65RR142 pKa = 11.84WLRR145 pKa = 11.84VNSSTIALNYY155 pKa = 9.16HH156 pKa = 5.9SLWSSPMLCLGLVV169 pKa = 3.35

Molecular weight: 18.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

959

0

959

275433

50

1815

287.2

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.366 ± 0.069

1.861 ± 0.039

5.471 ± 0.046

7.27 ± 0.074

4.077 ± 0.042

6.036 ± 0.065

2.338 ± 0.034

5.327 ± 0.056

7.012 ± 0.078

8.975 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.453 ± 0.036

4.652 ± 0.051

5.306 ± 0.088

3.961 ± 0.059

5.753 ± 0.071

7.277 ± 0.078

4.817 ± 0.047

6.527 ± 0.066

1.581 ± 0.049

2.941 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski