Streptococcus satellite phage Javan720

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZW38|A0A4D5ZW38_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan720 OX=2558814 GN=JavanS720_0023 PE=4 SV=1
MM1 pKa = 7.22TLDD4 pKa = 4.01LGKK7 pKa = 8.39MTQAEE12 pKa = 4.45FDD14 pKa = 3.72EE15 pKa = 4.66VMADD19 pKa = 3.21IKK21 pKa = 11.13ARR23 pKa = 11.84NPNLLQLITDD33 pKa = 4.52FLDD36 pKa = 3.62RR37 pKa = 11.84KK38 pKa = 8.24VTPEE42 pKa = 4.1EE43 pKa = 4.0VDD45 pKa = 3.88DD46 pKa = 4.23LLNMEE51 pKa = 4.45RR52 pKa = 11.84AEE54 pKa = 3.75QVEE57 pKa = 4.55YY58 pKa = 10.62IKK60 pKa = 10.79NYY62 pKa = 6.85QARR65 pKa = 11.84AA66 pKa = 3.37

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZYU9|A0A4D5ZYU9_9VIRU Transcriptional regulator OS=Streptococcus satellite phage Javan720 OX=2558814 GN=JavanS720_0004 PE=4 SV=1
MM1 pKa = 7.27GRR3 pKa = 11.84YY4 pKa = 7.19NTHH7 pKa = 6.77PAQGGGHH14 pKa = 4.78YY15 pKa = 10.27HH16 pKa = 7.73DD17 pKa = 4.02IKK19 pKa = 10.7LYY21 pKa = 8.35KK22 pKa = 9.91HH23 pKa = 6.74RR24 pKa = 11.84GRR26 pKa = 11.84SLEE29 pKa = 3.76QFQEE33 pKa = 3.91QKK35 pKa = 10.86LLKK38 pKa = 10.2KK39 pKa = 10.55FKK41 pKa = 10.35KK42 pKa = 9.66KK43 pKa = 9.86RR44 pKa = 11.84KK45 pKa = 9.08KK46 pKa = 10.61GRR48 pKa = 3.36

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

3808

35

496

146.5

17.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.2 ± 0.315

0.919 ± 0.144

5.567 ± 0.291

8.64 ± 0.517

4.307 ± 0.422

5.068 ± 0.418

1.681 ± 0.248

6.486 ± 0.398

9.27 ± 0.614

10.373 ± 0.501

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.252

5.488 ± 0.428

3.204 ± 0.386

3.834 ± 0.366

5.83 ± 0.35

5.252 ± 0.342

5.593 ± 0.612

5.357 ± 0.408

0.788 ± 0.218

4.517 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski