Human coronavirus 229E (HCoV-229E)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Duvinacovirus

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P19739|NS4A_CVH22 Non-structural protein 4a OS=Human coronavirus 229E OX=11137 GN=4a PE=3 SV=1
MM1 pKa = 7.16FVLLVAYY8 pKa = 9.84ALLHH12 pKa = 6.24IAGCQTTNGLNTSYY26 pKa = 10.55SVCNGCVGYY35 pKa = 10.09SEE37 pKa = 5.49NVFAVEE43 pKa = 4.01SGGYY47 pKa = 9.24IPSDD51 pKa = 3.14FAFNNWFLLTNTSSVVDD68 pKa = 3.68GVVRR72 pKa = 11.84SFQPLLLNCLWSVSGLRR89 pKa = 11.84FTTGFVYY96 pKa = 10.41FNGTGRR102 pKa = 11.84GDD104 pKa = 3.68CKK106 pKa = 10.91GFSSDD111 pKa = 3.57VLSDD115 pKa = 3.58VIRR118 pKa = 11.84YY119 pKa = 8.08NLNFEE124 pKa = 4.23EE125 pKa = 4.14NLRR128 pKa = 11.84RR129 pKa = 11.84GTILFKK135 pKa = 10.17TSYY138 pKa = 9.79GVVVFYY144 pKa = 8.57CTNNTLVSGDD154 pKa = 3.18AHH156 pKa = 7.49IPFGTVLGNFYY167 pKa = 10.84CFVNTTIGNEE177 pKa = 4.05TTSAFVGALPKK188 pKa = 9.64TVRR191 pKa = 11.84EE192 pKa = 4.1FVISRR197 pKa = 11.84TGHH200 pKa = 6.13FYY202 pKa = 10.78INGYY206 pKa = 10.46RR207 pKa = 11.84YY208 pKa = 7.33FTLGNVEE215 pKa = 3.93AVNFNVTTAEE225 pKa = 4.07TTDD228 pKa = 3.8FCTVALASYY237 pKa = 11.29ADD239 pKa = 3.47VLVNVSQTSIANIIYY254 pKa = 9.55CNSVINRR261 pKa = 11.84LRR263 pKa = 11.84CDD265 pKa = 3.35QLSFDD270 pKa = 4.48VPDD273 pKa = 4.15GFYY276 pKa = 9.65STSPIQSVEE285 pKa = 4.08LPVSIVSLPVYY296 pKa = 9.8HH297 pKa = 6.85KK298 pKa = 9.52HH299 pKa = 4.94TFIVLYY305 pKa = 10.81VDD307 pKa = 5.79FKK309 pKa = 10.03PQSGGGKK316 pKa = 9.91CFNCYY321 pKa = 8.96PAGVNITLANFNEE334 pKa = 4.85TKK336 pKa = 10.79GPLCVDD342 pKa = 4.0TSHH345 pKa = 6.91FTTKK349 pKa = 10.36YY350 pKa = 7.47VAVYY354 pKa = 10.46ANVGRR359 pKa = 11.84WSASINTGNCPFSFGKK375 pKa = 10.56VNNFVKK381 pKa = 10.67FGSVCFSLKK390 pKa = 10.45DD391 pKa = 3.56IPGGCAMPIVANWAYY406 pKa = 10.72SKK408 pKa = 11.02YY409 pKa = 8.53YY410 pKa = 10.08TIGSLYY416 pKa = 10.64VSWSDD421 pKa = 3.16GDD423 pKa = 4.5GITGVPQPVEE433 pKa = 4.29GVSSFMNVTLDD444 pKa = 2.87KK445 pKa = 9.64CTKK448 pKa = 9.1YY449 pKa = 10.83NIYY452 pKa = 10.19DD453 pKa = 3.42VSGVGVIRR461 pKa = 11.84VSNDD465 pKa = 2.51TFLNGITYY473 pKa = 9.16TSTSGNLLGFKK484 pKa = 10.26DD485 pKa = 3.78VTKK488 pKa = 9.86GTIYY492 pKa = 10.61SITPCNPPDD501 pKa = 3.49QLVVYY506 pKa = 8.2QQAVVGAMLSEE517 pKa = 4.28NFTSYY522 pKa = 11.02GFSNVVEE529 pKa = 4.31LPKK532 pKa = 10.59FFYY535 pKa = 10.75ASNGTYY541 pKa = 10.3NCTDD545 pKa = 2.86AVLTYY550 pKa = 11.05SSFGVCADD558 pKa = 3.43GSIIAVQPRR567 pKa = 11.84NVSYY571 pKa = 11.0DD572 pKa = 3.48SVSAIVTANLSIPSNWTTSVQVEE595 pKa = 4.12YY596 pKa = 10.83LQITSTPIVVDD607 pKa = 3.85CSTYY611 pKa = 9.47VCNGNVRR618 pKa = 11.84CVEE621 pKa = 4.02LLKK624 pKa = 10.78QYY626 pKa = 10.53TSACKK631 pKa = 9.83TIEE634 pKa = 4.11DD635 pKa = 4.03ALRR638 pKa = 11.84NSARR642 pKa = 11.84LEE644 pKa = 4.09SADD647 pKa = 3.53VSEE650 pKa = 5.05MLTFDD655 pKa = 3.73KK656 pKa = 11.09KK657 pKa = 11.1AFTLANVSSFGDD669 pKa = 3.69YY670 pKa = 10.6NLSSVIPSLPTSGSRR685 pKa = 11.84VAGRR689 pKa = 11.84SAIEE693 pKa = 5.09DD694 pKa = 3.32ILFSKK699 pKa = 10.7LVTSGLGTVDD709 pKa = 4.5ADD711 pKa = 3.86YY712 pKa = 11.22KK713 pKa = 11.03KK714 pKa = 9.54CTKK717 pKa = 10.13GLSIADD723 pKa = 4.44LACAQYY729 pKa = 11.34YY730 pKa = 9.2NGIMVLPGVADD741 pKa = 4.01AEE743 pKa = 4.12RR744 pKa = 11.84MAMYY748 pKa = 9.17TGSLIGGIALGGLTSAVSIPFSLAIQARR776 pKa = 11.84LNYY779 pKa = 9.7VALQTDD785 pKa = 4.22VLQEE789 pKa = 3.94NQKK792 pKa = 9.9ILAASFNKK800 pKa = 10.34AMTNIVDD807 pKa = 3.87AFTGVNDD814 pKa = 6.2AITQTSQALQTVATALNKK832 pKa = 10.09IQDD835 pKa = 3.83VVNQQGNSLNHH846 pKa = 6.06LTSQLRR852 pKa = 11.84QNFQAISSSIQAIYY866 pKa = 10.75DD867 pKa = 3.62RR868 pKa = 11.84LDD870 pKa = 3.71TIQADD875 pKa = 3.81QQVDD879 pKa = 3.53RR880 pKa = 11.84LITGRR885 pKa = 11.84LAALNVFVSHH895 pKa = 7.02TLTKK899 pKa = 8.3YY900 pKa = 8.64TEE902 pKa = 4.22VRR904 pKa = 11.84ASRR907 pKa = 11.84QLAQQKK913 pKa = 10.1VNEE916 pKa = 4.44CVKK919 pKa = 10.49SQSKK923 pKa = 10.49RR924 pKa = 11.84YY925 pKa = 8.36GFCGNGTHH933 pKa = 6.96IFSIVNAAPEE943 pKa = 4.27GLVFLHH949 pKa = 6.06TVLLPTQYY957 pKa = 11.19KK958 pKa = 10.38DD959 pKa = 3.27VEE961 pKa = 4.35AWSGLCVDD969 pKa = 4.6GTNGYY974 pKa = 7.64VLRR977 pKa = 11.84QPNLALYY984 pKa = 10.59KK985 pKa = 9.95EE986 pKa = 4.32GNYY989 pKa = 10.11YY990 pKa = 10.72RR991 pKa = 11.84ITSRR995 pKa = 11.84IMFEE999 pKa = 3.71PRR1001 pKa = 11.84IPTMADD1007 pKa = 3.54FVQIEE1012 pKa = 4.38NCNVTFVNISRR1023 pKa = 11.84SEE1025 pKa = 3.96LQTIVPEE1032 pKa = 4.33YY1033 pKa = 10.5IDD1035 pKa = 3.57VNKK1038 pKa = 9.33TLQEE1042 pKa = 3.95LSYY1045 pKa = 10.83KK1046 pKa = 10.05LPNYY1050 pKa = 7.31TVPDD1054 pKa = 3.84LVVEE1058 pKa = 4.42QYY1060 pKa = 11.18NQTILNLTSEE1070 pKa = 4.16ISTLEE1075 pKa = 4.02NKK1077 pKa = 9.98SAEE1080 pKa = 4.16LNYY1083 pKa = 9.73TVQKK1087 pKa = 10.55LQTLIDD1093 pKa = 4.68NINSTLVDD1101 pKa = 4.5LKK1103 pKa = 10.17WLNRR1107 pKa = 11.84VEE1109 pKa = 5.36TYY1111 pKa = 10.41IKK1113 pKa = 9.23WPWWVWLCISVVLIFVVSMLLLCCCSTGCCGFFSCFASSIRR1154 pKa = 11.84GCCEE1158 pKa = 3.71STKK1161 pKa = 10.6LPYY1164 pKa = 10.38YY1165 pKa = 10.29DD1166 pKa = 3.56VEE1168 pKa = 5.49KK1169 pKa = 10.86IHH1171 pKa = 6.47IQQ1173 pKa = 3.23

Molecular weight: 128.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P15422|VME1_CVH22 Membrane protein OS=Human coronavirus 229E OX=11137 GN=M PE=3 SV=3
MM1 pKa = 7.31ATVKK5 pKa = 9.76WADD8 pKa = 2.98ASEE11 pKa = 4.15PQRR14 pKa = 11.84GRR16 pKa = 11.84QGRR19 pKa = 11.84IPYY22 pKa = 9.97SLYY25 pKa = 10.99SPLLVDD31 pKa = 5.73SEE33 pKa = 4.75QPWKK37 pKa = 10.7VIPRR41 pKa = 11.84NLVPINKK48 pKa = 8.93KK49 pKa = 10.4DD50 pKa = 3.45KK51 pKa = 11.0NKK53 pKa = 10.73LIGYY57 pKa = 7.6WNVQKK62 pKa = 10.81RR63 pKa = 11.84FRR65 pKa = 11.84TRR67 pKa = 11.84KK68 pKa = 8.8GKK70 pKa = 10.37RR71 pKa = 11.84VDD73 pKa = 4.23LSPKK77 pKa = 9.28LHH79 pKa = 6.82FYY81 pKa = 11.19YY82 pKa = 10.7LGTGPHH88 pKa = 6.76KK89 pKa = 10.21DD90 pKa = 2.84AKK92 pKa = 10.2FRR94 pKa = 11.84EE95 pKa = 4.43RR96 pKa = 11.84VEE98 pKa = 4.13GVVWVAVDD106 pKa = 3.79GAKK109 pKa = 9.47TEE111 pKa = 3.99PTGYY115 pKa = 10.26GVRR118 pKa = 11.84RR119 pKa = 11.84KK120 pKa = 10.01NSEE123 pKa = 3.64PEE125 pKa = 3.74IPHH128 pKa = 6.81FNQKK132 pKa = 9.87LPNGVTVVEE141 pKa = 4.8EE142 pKa = 4.2PDD144 pKa = 3.07SRR146 pKa = 11.84APSRR150 pKa = 11.84SQSRR154 pKa = 11.84SQSRR158 pKa = 11.84GRR160 pKa = 11.84GEE162 pKa = 4.47SKK164 pKa = 9.89PQSRR168 pKa = 11.84NPSSDD173 pKa = 3.46RR174 pKa = 11.84NHH176 pKa = 6.45NSQDD180 pKa = 3.98DD181 pKa = 3.55IMKK184 pKa = 10.59AVAAALKK191 pKa = 10.67SLGFDD196 pKa = 3.67KK197 pKa = 10.89PQEE200 pKa = 4.05KK201 pKa = 10.13DD202 pKa = 3.04KK203 pKa = 11.44KK204 pKa = 9.11SAKK207 pKa = 9.64TGTPKK212 pKa = 10.47PSRR215 pKa = 11.84NQSPASSQTSAKK227 pKa = 10.2SLARR231 pKa = 11.84SQSSEE236 pKa = 3.88TKK238 pKa = 7.93EE239 pKa = 4.32QKK241 pKa = 10.46HH242 pKa = 5.47EE243 pKa = 4.05MQKK246 pKa = 10.2PRR248 pKa = 11.84WKK250 pKa = 10.22RR251 pKa = 11.84QPNDD255 pKa = 3.49DD256 pKa = 3.43VTSNVTQCFGPRR268 pKa = 11.84DD269 pKa = 3.6LDD271 pKa = 3.95HH272 pKa = 7.0NFGSAGVVANGVKK285 pKa = 10.53AKK287 pKa = 10.29GYY289 pKa = 8.25PQFAEE294 pKa = 4.56LVPSTAAMLFDD305 pKa = 3.29SHH307 pKa = 7.6IVSKK311 pKa = 10.89EE312 pKa = 3.77SGNTVVLTFTTRR324 pKa = 11.84VTVPKK329 pKa = 10.31DD330 pKa = 3.33HH331 pKa = 6.76PHH333 pKa = 6.16LGKK336 pKa = 10.32FLEE339 pKa = 4.34EE340 pKa = 3.99LNAFTRR346 pKa = 11.84EE347 pKa = 4.19MQQHH351 pKa = 6.7PLLNPSALEE360 pKa = 3.97FNPSQTSPATAEE372 pKa = 3.97PVRR375 pKa = 11.84DD376 pKa = 3.59EE377 pKa = 4.32VSIEE381 pKa = 3.68TDD383 pKa = 3.46IIDD386 pKa = 3.84EE387 pKa = 4.46VNN389 pKa = 2.86

Molecular weight: 43.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

12928

77

6758

1616.0

180.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.799 ± 0.205

3.349 ± 0.316

5.399 ± 0.411

4.223 ± 0.335

5.832 ± 0.2

6.443 ± 0.16

1.756 ± 0.143

5.268 ± 0.196

6.002 ± 0.602

8.609 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.242

5.716 ± 0.227

3.233 ± 0.316

2.924 ± 0.325

3.311 ± 0.264

7.008 ± 0.505

6.026 ± 0.477

10.164 ± 0.225

1.392 ± 0.185

4.409 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski