Caldalkalibacillus thermarum (strain TA2.A1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Caldalkalibacillus; Caldalkalibacillus thermarum

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2969 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5L525|F5L525_CALTT Uncharacterized protein OS=Caldalkalibacillus thermarum (strain TA2.A1) OX=986075 GN=CathTA2_0888 PE=4 SV=1
MM1 pKa = 7.33GRR3 pKa = 11.84ITLRR7 pKa = 11.84PEE9 pKa = 3.82LMTPGGQAASIMLDD23 pKa = 3.25DD24 pKa = 5.24CYY26 pKa = 11.24VGSFTMVYY34 pKa = 10.09RR35 pKa = 11.84EE36 pKa = 4.33NDD38 pKa = 3.9ALWGTIQLDD47 pKa = 3.92EE48 pKa = 4.65EE49 pKa = 4.28MLEE52 pKa = 4.13PDD54 pKa = 3.66EE55 pKa = 5.47KK56 pKa = 11.71GEE58 pKa = 3.98IDD60 pKa = 4.44LFMHH64 pKa = 6.06EE65 pKa = 5.05HH66 pKa = 6.75IEE68 pKa = 4.1DD69 pKa = 4.62LIEE72 pKa = 4.07ALQVRR77 pKa = 11.84EE78 pKa = 4.05CMITSTYY85 pKa = 10.87SDD87 pKa = 3.8YY88 pKa = 11.35DD89 pKa = 3.82YY90 pKa = 11.41VISTDD95 pKa = 4.16DD96 pKa = 3.21IVEE99 pKa = 3.97EE100 pKa = 4.21MIEE103 pKa = 3.93EE104 pKa = 4.31EE105 pKa = 4.13EE106 pKa = 4.23WVEE109 pKa = 4.11GEE111 pKa = 4.16EE112 pKa = 4.14AQEE115 pKa = 3.79LHH117 pKa = 6.44LSVVRR122 pKa = 11.84EE123 pKa = 3.8TRR125 pKa = 11.84HH126 pKa = 4.47RR127 pKa = 11.84VEE129 pKa = 4.13YY130 pKa = 10.28QIHH133 pKa = 6.92DD134 pKa = 3.87EE135 pKa = 4.03NHH137 pKa = 5.36EE138 pKa = 4.37VVAEE142 pKa = 3.9AVVYY146 pKa = 10.4KK147 pKa = 10.89SRR149 pKa = 11.84DD150 pKa = 3.49HH151 pKa = 5.54VTGEE155 pKa = 4.26VLWDD159 pKa = 4.01DD160 pKa = 4.45EE161 pKa = 4.3PTEE164 pKa = 4.8AEE166 pKa = 4.02MDD168 pKa = 3.74TVARR172 pKa = 11.84LIMDD176 pKa = 3.84EE177 pKa = 4.58CDD179 pKa = 3.65DD180 pKa = 4.3EE181 pKa = 4.68EE182 pKa = 4.76VEE184 pKa = 4.39SFSFVMYY191 pKa = 10.92VDD193 pKa = 3.91GEE195 pKa = 4.38EE196 pKa = 3.94IATIEE201 pKa = 4.4LTRR204 pKa = 11.84DD205 pKa = 3.38DD206 pKa = 5.28PIEE209 pKa = 4.01EE210 pKa = 4.71EE211 pKa = 3.9ITGEE215 pKa = 4.12EE216 pKa = 4.2VEE218 pKa = 4.81LDD220 pKa = 2.72IGQIEE225 pKa = 5.19GYY227 pKa = 10.63DD228 pKa = 3.48FTFDD232 pKa = 5.25LIRR235 pKa = 11.84EE236 pKa = 4.11EE237 pKa = 4.14MDD239 pKa = 3.36LLIYY243 pKa = 10.22HH244 pKa = 7.28IYY246 pKa = 10.73EE247 pKa = 4.06EE248 pKa = 4.33TDD250 pKa = 2.9EE251 pKa = 3.94EE252 pKa = 4.62RR253 pKa = 11.84IYY255 pKa = 11.04LGTATADD262 pKa = 3.98LSDD265 pKa = 4.99DD266 pKa = 3.88DD267 pKa = 4.16ATVLVEE273 pKa = 4.7FEE275 pKa = 4.16NPRR278 pKa = 11.84DD279 pKa = 3.43RR280 pKa = 11.84RR281 pKa = 11.84LRR283 pKa = 11.84EE284 pKa = 3.66QIVYY288 pKa = 10.26HH289 pKa = 6.85LIDD292 pKa = 3.86EE293 pKa = 4.9LEE295 pKa = 4.13EE296 pKa = 3.61EE297 pKa = 4.98AYY299 pKa = 10.62FDD301 pKa = 3.78TVTITMQYY309 pKa = 10.02DD310 pKa = 3.5DD311 pKa = 4.6EE312 pKa = 6.2VIDD315 pKa = 4.97EE316 pKa = 4.23YY317 pKa = 11.53HH318 pKa = 6.92FDD320 pKa = 3.81YY321 pKa = 11.04DD322 pKa = 3.89DD323 pKa = 3.65QPEE326 pKa = 4.06YY327 pKa = 11.47GLLDD331 pKa = 3.71GLTTEE336 pKa = 4.72NRR338 pKa = 11.84TEE340 pKa = 4.21TTVSSS345 pKa = 3.74

Molecular weight: 40.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5L555|F5L555_CALTT Spore cortex-lytic enzyme OS=Caldalkalibacillus thermarum (strain TA2.A1) OX=986075 GN=CathTA2_0918 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.8RR12 pKa = 11.84KK13 pKa = 8.27KK14 pKa = 8.7VHH16 pKa = 5.52GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.75VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.2KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2969

0

2969

837036

30

1541

281.9

31.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.946 ± 0.045

0.836 ± 0.015

4.847 ± 0.031

7.506 ± 0.052

4.024 ± 0.035

7.205 ± 0.044

2.53 ± 0.022

6.707 ± 0.038

5.717 ± 0.047

10.423 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.02

3.251 ± 0.027

4.245 ± 0.027

4.578 ± 0.038

5.38 ± 0.037

4.882 ± 0.03

5.058 ± 0.027

7.624 ± 0.04

1.181 ± 0.019

3.421 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski