Cellulophaga phage phi12:3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Helsingorvirus; Cellulophaga virus Cba121; Cellulophaga phage phi12:1

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZY40|R9ZY40_9CAUD N-acetylmuramoyl-L-alanine amidase OS=Cellulophaga phage phi12:3 OX=1327979 GN=Phi12:3_gp63 PE=4 SV=1
MM1 pKa = 7.7AYY3 pKa = 9.42IEE5 pKa = 4.88KK6 pKa = 10.32YY7 pKa = 8.06YY8 pKa = 11.0HH9 pKa = 6.52NYY11 pKa = 9.48CDD13 pKa = 4.92SFGTACKK20 pKa = 10.56VSILEE25 pKa = 3.88RR26 pKa = 11.84DD27 pKa = 3.82YY28 pKa = 11.38EE29 pKa = 4.46GSAIEE34 pKa = 5.48LEE36 pKa = 4.38AQFPPFKK43 pKa = 9.76KK44 pKa = 9.23TYY46 pKa = 10.67DD47 pKa = 3.41SDD49 pKa = 4.29SDD51 pKa = 4.0FKK53 pKa = 11.37FEE55 pKa = 5.35PIRR58 pKa = 11.84STSGEE63 pKa = 3.92FNIVFGTGNGIDD75 pKa = 3.8LSDD78 pKa = 4.29IEE80 pKa = 4.59TADD83 pKa = 3.23EE84 pKa = 4.0RR85 pKa = 11.84QYY87 pKa = 12.32KK88 pKa = 8.56MIHH91 pKa = 5.61YY92 pKa = 10.39VDD94 pKa = 5.44GSVDD98 pKa = 3.2WIGYY102 pKa = 6.7VVPDD106 pKa = 4.36GFSHH110 pKa = 6.42EE111 pKa = 4.3LRR113 pKa = 11.84GGIYY117 pKa = 10.11FGSLIATDD125 pKa = 4.38GLKK128 pKa = 10.0QLQSLPFVDD137 pKa = 4.77ISGDD141 pKa = 3.47NYY143 pKa = 11.0GLQDD147 pKa = 3.33LTYY150 pKa = 9.27NNGYY154 pKa = 9.15EE155 pKa = 4.37FPFSLVLTEE164 pKa = 4.48ILRR167 pKa = 11.84KK168 pKa = 9.71LDD170 pKa = 3.77LDD172 pKa = 4.49LNTWTAVDD180 pKa = 4.03VYY182 pKa = 9.62EE183 pKa = 5.82KK184 pKa = 11.49NMTQLGNSRR193 pKa = 11.84DD194 pKa = 4.0SDD196 pKa = 4.3PLSQAYY202 pKa = 10.27VNVKK206 pKa = 9.55TYY208 pKa = 10.92INDD211 pKa = 3.56TTRR214 pKa = 11.84KK215 pKa = 10.04DD216 pKa = 3.07IPYY219 pKa = 9.32WADD222 pKa = 2.85AQEE225 pKa = 4.08AMNCFEE231 pKa = 5.04VLNNMCTLFGARR243 pKa = 11.84VSQNKK248 pKa = 9.11GVWTFKK254 pKa = 10.45RR255 pKa = 11.84VNIDD259 pKa = 3.1ANYY262 pKa = 10.74GSGDD266 pKa = 3.46TQRR269 pKa = 11.84YY270 pKa = 3.85WRR272 pKa = 11.84KK273 pKa = 9.08YY274 pKa = 7.57NTLSVYY280 pKa = 10.79LGRR283 pKa = 11.84EE284 pKa = 4.46AINDD288 pKa = 3.89TVNIPCSDD296 pKa = 3.25IDD298 pKa = 3.77EE299 pKa = 4.4VLIGTDD305 pKa = 3.53HH306 pKa = 6.8QISISDD312 pKa = 3.29VYY314 pKa = 10.65AAYY317 pKa = 10.75RR318 pKa = 11.84MNYY321 pKa = 8.79KK322 pKa = 10.04FQLVRR327 pKa = 11.84EE328 pKa = 4.28GDD330 pKa = 3.65TPLNLLTNGNFSVFNNTSPFAAPQDD355 pKa = 3.26WFLWSEE361 pKa = 4.01GTRR364 pKa = 11.84WVPRR368 pKa = 11.84ISPVTLSPTDD378 pKa = 3.27AGGITTAVEE387 pKa = 4.72FGTLTPGLSTNNTDD401 pKa = 3.71PVGQVWASLRR411 pKa = 11.84YY412 pKa = 9.62YY413 pKa = 10.17EE414 pKa = 4.36NKK416 pKa = 10.15SVNEE420 pKa = 3.55GDD422 pKa = 3.84ALYY425 pKa = 8.37FTAWAKK431 pKa = 10.77FGFNNATNPEE441 pKa = 3.88NVYY444 pKa = 11.02APFLRR449 pKa = 11.84CVIVGASGKK458 pKa = 10.22KK459 pKa = 10.03YY460 pKa = 10.57YY461 pKa = 10.3LANNLSDD468 pKa = 5.85EE469 pKa = 4.06PLKK472 pKa = 10.47WINNDD477 pKa = 4.01DD478 pKa = 3.78FDD480 pKa = 4.31TAKK483 pKa = 11.0DD484 pKa = 3.53DD485 pKa = 4.13LFFVLLYY492 pKa = 10.23GFAVGAPEE500 pKa = 4.18SSDD503 pKa = 2.84ISSEE507 pKa = 3.81FGVYY511 pKa = 8.04GWKK514 pKa = 10.26EE515 pKa = 3.62FALTLPEE522 pKa = 4.22IPEE525 pKa = 4.76DD526 pKa = 3.67GLLTFDD532 pKa = 3.37VHH534 pKa = 7.53GLAKK538 pKa = 10.6FEE540 pKa = 4.33GKK542 pKa = 8.45ATNSFAKK549 pKa = 10.2IKK551 pKa = 10.87LNEE554 pKa = 3.94QKK556 pKa = 10.72SNKK559 pKa = 8.9FIYY562 pKa = 9.23WYY564 pKa = 9.32PVRR567 pKa = 11.84EE568 pKa = 4.09GNYY571 pKa = 9.77DD572 pKa = 3.44NGVGRR577 pKa = 11.84LQITGLSLGTIPDD590 pKa = 3.86PNEE593 pKa = 3.41NAEE596 pKa = 4.13AQDD599 pKa = 3.54YY600 pKa = 11.01VYY602 pKa = 11.45YY603 pKa = 10.34NQNKK607 pKa = 9.4NYY609 pKa = 9.67SLEE612 pKa = 4.06VDD614 pKa = 3.52PVEE617 pKa = 4.47VLNGDD622 pKa = 3.72ILDD625 pKa = 3.82QNHH628 pKa = 5.65VSRR631 pKa = 11.84IIVPSNTTGNKK642 pKa = 9.68NFWDD646 pKa = 4.61TIDD649 pKa = 3.63NKK651 pKa = 11.1YY652 pKa = 10.93GGASLGLITVKK663 pKa = 10.59SIMNLYY669 pKa = 9.65FKK671 pKa = 10.42PFQLLEE677 pKa = 4.6GDD679 pKa = 4.38LKK681 pKa = 11.37SQFGNADD688 pKa = 3.18TVYY691 pKa = 9.66TFEE694 pKa = 5.67AIPGKK699 pKa = 9.21SFCLLRR705 pKa = 11.84GTFIQKK711 pKa = 9.98NNYY714 pKa = 8.86IEE716 pKa = 4.41GATFFEE722 pKa = 4.8ITSEE726 pKa = 4.56DD727 pKa = 3.39IPAGGTEE734 pKa = 4.59GGNSLDD740 pKa = 3.55AEE742 pKa = 4.5YY743 pKa = 11.33VLTGRR748 pKa = 11.84KK749 pKa = 8.99RR750 pKa = 11.84CQKK753 pKa = 10.16VDD755 pKa = 3.44NLNTGLAEE763 pKa = 4.46VEE765 pKa = 4.03LDD767 pKa = 3.54DD768 pKa = 5.54VNINSEE774 pKa = 4.6TYY776 pKa = 10.37GQTIWEE782 pKa = 4.31TSAVLGLTLCPIGEE796 pKa = 4.07PDD798 pKa = 3.16KK799 pKa = 11.19YY800 pKa = 11.47YY801 pKa = 10.28WGTDD805 pKa = 3.06SAVYY809 pKa = 10.31DD810 pKa = 3.59SSTFDD815 pKa = 2.93SVGFYY820 pKa = 10.99EE821 pKa = 4.5EE822 pKa = 5.22DD823 pKa = 3.5GGDD826 pKa = 3.46EE827 pKa = 4.31VSCEE831 pKa = 3.81FTNPGGEE838 pKa = 3.95YY839 pKa = 10.59VYY841 pKa = 10.45FLHH844 pKa = 6.9LASLGLVEE852 pKa = 5.44SIEE855 pKa = 4.29TEE857 pKa = 3.93AQDD860 pKa = 4.91EE861 pKa = 4.67IISDD865 pKa = 4.07FQYY868 pKa = 10.26LTDD871 pKa = 3.63VTINSYY877 pKa = 10.54LYY879 pKa = 9.95RR880 pKa = 11.84VLRR883 pKa = 11.84QNYY886 pKa = 5.34VTSVFNDD893 pKa = 3.04IQITFKK899 pKa = 11.02FSS901 pKa = 2.78

Molecular weight: 101.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A0Y5|S0A0Y5_9CAUD VirE N-domain protein OS=Cellulophaga phage phi12:3 OX=1327979 GN=Phi12:3_gp21 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.21ISEE5 pKa = 4.33TKK7 pKa = 10.05KK8 pKa = 10.49RR9 pKa = 11.84EE10 pKa = 4.0GVYY13 pKa = 10.45CCAYY17 pKa = 9.07GCKK20 pKa = 9.94GHH22 pKa = 7.02PEE24 pKa = 3.77QRR26 pKa = 11.84KK27 pKa = 9.5GMLCHH32 pKa = 6.11KK33 pKa = 10.03HH34 pKa = 4.72YY35 pKa = 10.97ARR37 pKa = 11.84LMRR40 pKa = 11.84EE41 pKa = 3.98RR42 pKa = 11.84SPKK45 pKa = 9.29KK46 pKa = 10.32VRR48 pKa = 11.84YY49 pKa = 9.11SQAKK53 pKa = 8.88QKK55 pKa = 10.77AKK57 pKa = 10.7SRR59 pKa = 11.84GIDD62 pKa = 3.47FTITLEE68 pKa = 3.88WFLRR72 pKa = 11.84FCDD75 pKa = 3.25RR76 pKa = 11.84TGYY79 pKa = 8.75MSKK82 pKa = 10.47GRR84 pKa = 11.84RR85 pKa = 11.84GQNATLDD92 pKa = 3.82RR93 pKa = 11.84RR94 pKa = 11.84CNLHH98 pKa = 7.13GYY100 pKa = 9.53HH101 pKa = 6.3SWNIQILTNRR111 pKa = 11.84QNASKK116 pKa = 10.71GNRR119 pKa = 11.84PSGEE123 pKa = 4.23HH124 pKa = 6.38FDD126 pKa = 4.52CPFF129 pKa = 4.35

Molecular weight: 15.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12570

33

901

196.4

22.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.165 ± 0.47

1.082 ± 0.127

6.667 ± 0.21

6.547 ± 0.274

4.678 ± 0.221

6.364 ± 0.426

1.329 ± 0.156

7.804 ± 0.283

8.958 ± 0.61

8.234 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.578 ± 0.227

5.831 ± 0.286

3.333 ± 0.211

3.532 ± 0.213

3.723 ± 0.221

6.929 ± 0.326

5.306 ± 0.364

5.442 ± 0.199

1.392 ± 0.123

4.105 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski