Arboretum almendravirus
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X4QQM2|X4QQM2_9RHAB SH protein OS=Arboretum almendravirus OX=1972683 PE=4 SV=1
MM1 pKa = 7.6 SNPKK5 pKa = 9.93 VCDD8 pKa = 3.53 DD9 pKa = 4.29 LFNSRR14 pKa = 11.84 RR15 pKa = 11.84 MLAQIDD21 pKa = 3.78 VSAFDD26 pKa = 3.26 ISKK29 pKa = 10.57 QISEE33 pKa = 4.92 DD34 pKa = 3.57 YY35 pKa = 9.26 GTTRR39 pKa = 11.84 GTDD42 pKa = 3.25 EE43 pKa = 5.29 LNDD46 pKa = 3.25 IEE48 pKa = 4.75 YY49 pKa = 10.98 EE50 pKa = 4.05 EE51 pKa = 4.82 NEE53 pKa = 4.19 EE54 pKa = 4.97 KK55 pKa = 10.62 KK56 pKa = 10.96 GLEE59 pKa = 3.73 NDD61 pKa = 3.22 KK62 pKa = 10.51 TKK64 pKa = 10.18 TLTGDD69 pKa = 3.86 EE70 pKa = 4.25 IMTPNIFSRR79 pKa = 11.84 MINTPLEE86 pKa = 4.21 KK87 pKa = 10.8 ANLEE91 pKa = 4.0 LSEE94 pKa = 4.47 TKK96 pKa = 10.3 CRR98 pKa = 11.84 NNKK101 pKa = 9.13 PCVEE105 pKa = 3.94 PRR107 pKa = 11.84 QVTFNTGRR115 pKa = 11.84 STEE118 pKa = 4.27 SNDD121 pKa = 3.24 GNKK124 pKa = 10.02 SYY126 pKa = 11.55 EE127 pKa = 4.04 EE128 pKa = 4.91 GYY130 pKa = 10.82 LRR132 pKa = 11.84 AIWDD136 pKa = 3.68 INNLLSKK143 pKa = 10.68 EE144 pKa = 4.01 NFKK147 pKa = 10.47 MEE149 pKa = 3.87 ITRR152 pKa = 11.84 DD153 pKa = 3.67 PKK155 pKa = 11.12 GSLTIKK161 pKa = 9.15 KK162 pKa = 8.43 TNDD165 pKa = 2.92 SGNVEE170 pKa = 4.07 KK171 pKa = 10.79 CIEE174 pKa = 4.01 LAKK177 pKa = 10.4 SDD179 pKa = 4.32 EE180 pKa = 4.49 SSCSYY185 pKa = 9.16 KK186 pKa = 10.44 TEE188 pKa = 3.7 IDD190 pKa = 3.22 QDD192 pKa = 3.3 ISFTDD197 pKa = 3.71 SEE199 pKa = 5.0 SCGVYY204 pKa = 10.23 PPLHH208 pKa = 6.44 YY209 pKa = 11.11 DD210 pKa = 3.54 LMFGGKK216 pKa = 8.97 VEE218 pKa = 4.32 INVKK222 pKa = 10.34 VLVEE226 pKa = 4.04 EE227 pKa = 4.67 LKK229 pKa = 10.97 SSNMDD234 pKa = 3.36 KK235 pKa = 11.12 DD236 pKa = 3.59 DD237 pKa = 4.27 LKK239 pKa = 11.05 IVIQLIDD246 pKa = 3.62 NNLEE250 pKa = 3.83 NKK252 pKa = 8.75 NAKK255 pKa = 10.17 DD256 pKa = 2.83 ILKK259 pKa = 10.03 YY260 pKa = 10.13 IKK262 pKa = 10.02 HH263 pKa = 5.77 SKK265 pKa = 9.79 QYY267 pKa = 10.99 GKK269 pKa = 9.79 IVYY272 pKa = 9.21 QVARR276 pKa = 11.84 KK277 pKa = 9.82 YY278 pKa = 10.79 KK279 pKa = 9.45 II280 pKa = 3.25
Molecular weight: 32.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.014
IPC2_protein 5.067
IPC_protein 4.978
Toseland 4.851
ProMoST 5.08
Dawson 4.927
Bjellqvist 5.067
Wikipedia 4.787
Rodwell 4.838
Grimsley 4.762
Solomon 4.927
Lehninger 4.876
Nozaki 5.029
DTASelect 5.181
Thurlkill 4.838
EMBOSS 4.8
Sillero 5.105
Patrickios 4.177
IPC_peptide 4.94
IPC2_peptide 5.092
IPC2.peptide.svr19 5.148
Protein with the highest isoelectric point:
>tr|X4R558|X4R558_9RHAB G protein OS=Arboretum almendravirus OX=1972683 PE=4 SV=1
MM1 pKa = 7.67 LANQFIMNNKK11 pKa = 9.35 YY12 pKa = 9.44 VFKK15 pKa = 9.94 TFRR18 pKa = 11.84 IRR20 pKa = 11.84 YY21 pKa = 5.81 EE22 pKa = 3.83 ASFKK26 pKa = 10.44 EE27 pKa = 4.54 LPPHH31 pKa = 7.35 SDD33 pKa = 3.85 FKK35 pKa = 11.06 PILDD39 pKa = 4.18 IYY41 pKa = 10.48 DD42 pKa = 3.75 GKK44 pKa = 10.91 IKK46 pKa = 10.34 FYY48 pKa = 11.04 EE49 pKa = 4.11 VFTFIINYY57 pKa = 9.4 LLHH60 pKa = 6.04 LQIRR64 pKa = 11.84 NKK66 pKa = 9.69 VMRR69 pKa = 11.84 SHH71 pKa = 7.75 DD72 pKa = 3.89 STFVMCFPVDD82 pKa = 3.63 EE83 pKa = 4.4 TWTYY87 pKa = 11.1 RR88 pKa = 11.84 RR89 pKa = 11.84 DD90 pKa = 3.98 DD91 pKa = 3.49 IYY93 pKa = 11.59 SFHH96 pKa = 7.78 KK97 pKa = 10.04 DD98 pKa = 2.55 IQMVVGCEE106 pKa = 3.84 VRR108 pKa = 11.84 EE109 pKa = 3.82 LRR111 pKa = 11.84 LRR113 pKa = 11.84 VIIDD117 pKa = 3.38 MNTNNLPRR125 pKa = 11.84 SRR127 pKa = 11.84 LVRR130 pKa = 11.84 DD131 pKa = 3.66 VLRR134 pKa = 11.84 KK135 pKa = 7.78 TLSRR139 pKa = 11.84 LYY141 pKa = 10.86 DD142 pKa = 3.47 PAISSKK148 pKa = 9.4 MYY150 pKa = 10.47 EE151 pKa = 4.03 VIKK154 pKa = 10.51 RR155 pKa = 11.84 QNEE158 pKa = 4.18 CC159 pKa = 3.56
Molecular weight: 19.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.81
IPC2_protein 8.785
IPC_protein 8.77
Toseland 9.224
ProMoST 9.136
Dawson 9.56
Bjellqvist 9.385
Wikipedia 9.765
Rodwell 9.706
Grimsley 9.648
Solomon 9.633
Lehninger 9.589
Nozaki 9.414
DTASelect 9.311
Thurlkill 9.399
EMBOSS 9.677
Sillero 9.545
Patrickios 4.673
IPC_peptide 9.619
IPC2_peptide 8.156
IPC2.peptide.svr19 7.763
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3484
73
2062
580.7
67.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.641 ± 0.493
1.464 ± 0.332
6.544 ± 0.437
6.228 ± 0.586
4.65 ± 0.497
4.277 ± 0.393
2.067 ± 0.292
8.927 ± 0.293
8.266 ± 0.621
9.816 ± 0.749
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.408
7.836 ± 0.377
3.243 ± 0.246
2.612 ± 0.278
4.908 ± 0.487
7.52 ± 0.168
4.822 ± 0.231
5.138 ± 0.371
1.234 ± 0.212
5.253 ± 0.347
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here