Sanxia water strider virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEV2|A0A1L3KEV2_9VIRU Uncharacterized protein OS=Sanxia water strider virus 10 OX=1923394 PE=4 SV=1
MM1 pKa = 7.66QEE3 pKa = 4.12LLSGKK8 pKa = 9.29EE9 pKa = 3.92VADD12 pKa = 5.18DD13 pKa = 2.94IHH15 pKa = 8.33VFVKK19 pKa = 10.12QEE21 pKa = 3.53PHH23 pKa = 6.1KK24 pKa = 10.39RR25 pKa = 11.84AKK27 pKa = 10.36LDD29 pKa = 3.22EE30 pKa = 4.11GRR32 pKa = 11.84YY33 pKa = 9.24RR34 pKa = 11.84LISAVSAVDD43 pKa = 3.41TMVDD47 pKa = 3.84RR48 pKa = 11.84ILFGPLQAKK57 pKa = 9.44VLEE60 pKa = 4.79TVGQTPTMIGWTPLQGGYY78 pKa = 8.4RR79 pKa = 11.84HH80 pKa = 6.13FRR82 pKa = 11.84RR83 pKa = 11.84MLAKK87 pKa = 9.87RR88 pKa = 11.84VMCADD93 pKa = 3.3KK94 pKa = 11.14SSWDD98 pKa = 3.22WTVPGWLVDD107 pKa = 4.02FWEE110 pKa = 4.8DD111 pKa = 3.83FVLSLCVDD119 pKa = 3.45APEE122 pKa = 3.47WWQRR126 pKa = 11.84LVRR129 pKa = 11.84MRR131 pKa = 11.84FRR133 pKa = 11.84MLFSEE138 pKa = 5.22AVFQFADD145 pKa = 3.93GTRR148 pKa = 11.84VKK150 pKa = 10.74QPVKK154 pKa = 10.55GVMKK158 pKa = 10.28SGCLLTILLNSISQVIIHH176 pKa = 6.44VIVQLLLGNDD186 pKa = 3.46PMEE189 pKa = 4.29GLPYY193 pKa = 10.64NIGDD197 pKa = 3.89DD198 pKa = 3.61TTQAFLEE205 pKa = 4.25YY206 pKa = 9.83FRR208 pKa = 11.84EE209 pKa = 4.11YY210 pKa = 10.16FAEE213 pKa = 4.01IEE215 pKa = 4.12KK216 pKa = 10.89LGIRR220 pKa = 11.84VKK222 pKa = 9.89EE223 pKa = 4.04VKK225 pKa = 10.1IQDD228 pKa = 3.25WVEE231 pKa = 3.55FAGFYY236 pKa = 10.16IGCSEE241 pKa = 4.12TWPVYY246 pKa = 8.65WEE248 pKa = 3.69KK249 pKa = 10.83HH250 pKa = 5.54LYY252 pKa = 9.71SVAHH256 pKa = 6.11QEE258 pKa = 5.09DD259 pKa = 3.9KK260 pKa = 11.22LLPQTLFMYY269 pKa = 10.65QMLYY273 pKa = 10.38AAQPSMLSFIRR284 pKa = 11.84KK285 pKa = 8.48HH286 pKa = 6.1LKK288 pKa = 10.08EE289 pKa = 4.81LDD291 pKa = 3.5PTLCKK296 pKa = 9.99PLWVLRR302 pKa = 11.84GYY304 pKa = 11.28VDD306 pKa = 3.17GTLPLHH312 pKa = 6.25ITSNWII318 pKa = 3.15

Molecular weight: 36.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEU6|A0A1L3KEU6_9VIRU RNA-directed RNA polymerase OS=Sanxia water strider virus 10 OX=1923394 PE=4 SV=1
MM1 pKa = 7.59PPKK4 pKa = 10.12SVNPIKK10 pKa = 10.57EE11 pKa = 4.35RR12 pKa = 11.84ICPYY16 pKa = 10.07CGSKK20 pKa = 10.84FMGKK24 pKa = 8.98RR25 pKa = 11.84AMMTHH30 pKa = 7.48LLSAHH35 pKa = 6.81PGSSQTKK42 pKa = 9.41NRR44 pKa = 11.84AQAAPKK50 pKa = 8.4PQRR53 pKa = 11.84VKK55 pKa = 11.09AKK57 pKa = 9.86GPSGSNVARR66 pKa = 11.84VASGRR71 pKa = 11.84DD72 pKa = 3.15IIATVSVASTKK83 pKa = 10.22SVGYY87 pKa = 10.08NVVEE91 pKa = 4.19LFVNPRR97 pKa = 11.84LLPSTRR103 pKa = 11.84LCQEE107 pKa = 3.6SKK109 pKa = 8.9MWSRR113 pKa = 11.84WLPVTLMVEE122 pKa = 4.26IRR124 pKa = 11.84TSSPKK129 pKa = 10.18IIGGMYY135 pKa = 8.74IAAWTPDD142 pKa = 3.26SKK144 pKa = 11.72EE145 pKa = 4.02NLPSGADD152 pKa = 3.19AVRR155 pKa = 11.84RR156 pKa = 11.84LATWPRR162 pKa = 11.84SLEE165 pKa = 3.97RR166 pKa = 11.84HH167 pKa = 5.53VSTDD171 pKa = 2.85SQFQIPVDD179 pKa = 4.31ASRR182 pKa = 11.84KK183 pKa = 8.75WYY185 pKa = 8.97TFDD188 pKa = 5.95SGDD191 pKa = 3.58QTDD194 pKa = 3.77TTHH197 pKa = 7.55GIFVLLLSAPISGLTGDD214 pKa = 3.81IQFSVHH220 pKa = 5.63LHH222 pKa = 4.0WTIKK226 pKa = 10.42FDD228 pKa = 4.01GPEE231 pKa = 4.15LEE233 pKa = 4.52MSGPQPGDD241 pKa = 3.64FIYY244 pKa = 10.83ADD246 pKa = 3.65SGYY249 pKa = 8.79TPYY252 pKa = 10.71FSDD255 pKa = 5.34SSNDD259 pKa = 2.84WWGGKK264 pKa = 8.55YY265 pKa = 10.43LSLKK269 pKa = 8.18HH270 pKa = 5.89TNGGSMVPFPTAKK283 pKa = 9.86HH284 pKa = 5.5DD285 pKa = 3.85VVYY288 pKa = 10.78KK289 pKa = 8.3CTEE292 pKa = 4.31PIGYY296 pKa = 9.21YY297 pKa = 10.07KK298 pKa = 11.11SNGTLHH304 pKa = 5.92NASYY308 pKa = 11.13GVLAKK313 pKa = 10.66NYY315 pKa = 8.01TSYY318 pKa = 11.62NIMALFVSKK327 pKa = 10.61EE328 pKa = 3.82DD329 pKa = 3.13AFKK332 pKa = 10.57YY333 pKa = 10.36VKK335 pKa = 10.69DD336 pKa = 3.7GGEE339 pKa = 3.85TGLLQFYY346 pKa = 10.99SDD348 pKa = 4.89GPWVSPPNPAWEE360 pKa = 4.28TVPVPPTTIEE370 pKa = 4.04VPGFTNRR377 pKa = 11.84RR378 pKa = 11.84IALDD382 pKa = 3.39RR383 pKa = 11.84VDD385 pKa = 5.45RR386 pKa = 11.84DD387 pKa = 3.28ILLEE391 pKa = 3.99TLFQRR396 pKa = 11.84ISSLEE401 pKa = 3.59EE402 pKa = 4.05RR403 pKa = 11.84IRR405 pKa = 11.84SLQVTPASSTSSSMIVLHH423 pKa = 6.17EE424 pKa = 4.29PPVEE428 pKa = 4.01EE429 pKa = 4.44EE430 pKa = 4.26SLNRR434 pKa = 11.84PPGPDD439 pKa = 2.87SS440 pKa = 3.52

Molecular weight: 48.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1328

318

570

442.7

49.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.702 ± 0.8

1.205 ± 0.135

5.045 ± 0.199

4.819 ± 0.351

4.292 ± 0.365

6.25 ± 0.332

2.033 ± 0.235

4.593 ± 0.378

5.271 ± 0.124

9.639 ± 0.978

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.937 ± 0.26

2.56 ± 0.432

6.551 ± 0.866

3.389 ± 0.495

5.045 ± 0.158

8.208 ± 1.316

6.401 ± 0.51

8.283 ± 0.511

2.711 ± 0.32

4.066 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski