Sugarcane striate virus
Average proteome isoelectric point is 7.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A218KH22|A0A218KH22_9GEMI Capsid protein OS=Sugarcane striate virus OX=1868659 PE=3 SV=1
MM1 pKa = 7.36 RR2 pKa = 11.84 TNANAVRR9 pKa = 11.84 RR10 pKa = 11.84 VEE12 pKa = 4.94 FMTTPATTEE21 pKa = 4.01 DD22 pKa = 3.79 AFQSGTQEE30 pKa = 4.31 SSDD33 pKa = 3.85 SPSEE37 pKa = 4.44 PIHH40 pKa = 5.16 THH42 pKa = 3.12 QTTWKK47 pKa = 9.41 RR48 pKa = 11.84 NFEE51 pKa = 4.06 FRR53 pKa = 11.84 SCNAFLTYY61 pKa = 9.53 PRR63 pKa = 11.84 CALDD67 pKa = 3.57 CEE69 pKa = 4.63 YY70 pKa = 11.46 VLDD73 pKa = 4.73 LPVGSYY79 pKa = 7.89 VFRR82 pKa = 11.84 WSPIYY87 pKa = 10.88 ALVSAEE93 pKa = 3.76 NHH95 pKa = 5.26 QDD97 pKa = 3.26 GGIHH101 pKa = 6.03 LHH103 pKa = 6.7 CLIQTLGRR111 pKa = 11.84 ITTRR115 pKa = 11.84 DD116 pKa = 3.27 PAFFDD121 pKa = 5.1 LDD123 pKa = 3.73 NQYY126 pKa = 10.86 HH127 pKa = 6.7 PNIQAARR134 pKa = 11.84 SPDD137 pKa = 3.22 NTRR140 pKa = 11.84 DD141 pKa = 3.81 YY142 pKa = 11.06 ILKK145 pKa = 10.47 DD146 pKa = 3.85 PISHH150 pKa = 5.64 VEE152 pKa = 3.81 RR153 pKa = 11.84 GTFQPRR159 pKa = 11.84 RR160 pKa = 11.84 GNTRR164 pKa = 11.84 NPGDD168 pKa = 3.75 RR169 pKa = 11.84 GSGSKK174 pKa = 10.26 DD175 pKa = 3.09 AIMKK179 pKa = 9.31 EE180 pKa = 3.93 IFRR183 pKa = 11.84 TATSKK188 pKa = 10.91 EE189 pKa = 3.99 DD190 pKa = 3.83 YY191 pKa = 10.93 LRR193 pKa = 11.84 MIQKK197 pKa = 9.61 SFPFEE202 pKa = 3.95 WAKK205 pKa = 10.73 HH206 pKa = 4.91 LSQFEE211 pKa = 3.98 YY212 pKa = 10.21 SANYY216 pKa = 9.33 LFPPVPEE223 pKa = 4.46 PFNNDD228 pKa = 3.09 FPEE231 pKa = 4.38 TEE233 pKa = 4.15 PDD235 pKa = 4.28 LICRR239 pKa = 11.84 EE240 pKa = 4.27 VLDD243 pKa = 4.04 EE244 pKa = 4.16 WQHH247 pKa = 6.67 DD248 pKa = 3.83 NLFQVTPYY256 pKa = 10.63 TYY258 pKa = 10.09 MLANHH263 pKa = 7.04 HH264 pKa = 6.13 CTSIEE269 pKa = 3.95 EE270 pKa = 4.3 ATSDD274 pKa = 3.97 LQWMADD280 pKa = 3.28 HH281 pKa = 7.05 ARR283 pKa = 11.84 TLRR286 pKa = 11.84 SSTIGNRR293 pKa = 11.84 ASTSLDD299 pKa = 3.21 LQGQEE304 pKa = 4.01 RR305 pKa = 11.84 QPGPEE310 pKa = 3.6 AA311 pKa = 4.32
Molecular weight: 35.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.269
IPC2_protein 5.258
IPC_protein 5.245
Toseland 5.461
ProMoST 5.499
Dawson 5.372
Bjellqvist 5.448
Wikipedia 5.321
Rodwell 5.321
Grimsley 5.512
Solomon 5.372
Lehninger 5.347
Nozaki 5.575
DTASelect 5.741
Thurlkill 5.575
EMBOSS 5.55
Sillero 5.652
Patrickios 3.884
IPC_peptide 5.385
IPC2_peptide 5.664
IPC2.peptide.svr19 5.576
Protein with the highest isoelectric point:
>tr|A0A218KH31|A0A218KH31_9GEMI Replication-associated protein OS=Sugarcane striate virus OX=1868659 PE=3 SV=1
MM1 pKa = 7.61 SSRR4 pKa = 11.84 GPSRR8 pKa = 11.84 KK9 pKa = 9.25 RR10 pKa = 11.84 PRR12 pKa = 11.84 SSTRR16 pKa = 11.84 VVKK19 pKa = 10.11 WPSEE23 pKa = 3.88 ALRR26 pKa = 11.84 KK27 pKa = 10.25 GYY29 pKa = 8.32 TPYY32 pKa = 11.08 NSYY35 pKa = 11.18 LRR37 pKa = 11.84 GTKK40 pKa = 9.64 SGRR43 pKa = 11.84 SDD45 pKa = 3.22 KK46 pKa = 10.99 RR47 pKa = 11.84 PNLQVQTFYY56 pKa = 11.63 ACGPTQVQVKK66 pKa = 10.19 KK67 pKa = 10.9 GGIVNLLNGYY77 pKa = 7.14 TRR79 pKa = 11.84 GSDD82 pKa = 3.09 DD83 pKa = 3.9 NQRR86 pKa = 11.84 HH87 pKa = 4.27 TCEE90 pKa = 3.98 TITYY94 pKa = 9.46 KK95 pKa = 10.93 LSLDD99 pKa = 3.36 LHH101 pKa = 7.78 INIATDD107 pKa = 3.86 LFKK110 pKa = 11.35 NCARR114 pKa = 11.84 GTGVIWLIYY123 pKa = 8.55 DD124 pKa = 3.99 TSPTGTQLTTQQIFAYY140 pKa = 9.16 PDD142 pKa = 3.47 ALSLYY147 pKa = 8.8 PYY149 pKa = 7.71 TWKK152 pKa = 10.51 VARR155 pKa = 11.84 DD156 pKa = 3.32 QCHH159 pKa = 5.62 RR160 pKa = 11.84 FIIKK164 pKa = 10.15 RR165 pKa = 11.84 RR166 pKa = 11.84 WTFRR170 pKa = 11.84 LEE172 pKa = 4.29 SNGTLGSSDD181 pKa = 3.89 YY182 pKa = 10.54 SSKK185 pKa = 10.84 PIAPCNNNVYY195 pKa = 10.07 FSKK198 pKa = 10.23 FVKK201 pKa = 10.3 RR202 pKa = 11.84 LGVRR206 pKa = 11.84 TEE208 pKa = 3.85 WKK210 pKa = 8.43 NTTGGDD216 pKa = 3.48 AGDD219 pKa = 3.88 MKK221 pKa = 11.13 SGGLFLAFAAGNGMLFNVDD240 pKa = 3.02 GTTRR244 pKa = 11.84 LYY246 pKa = 10.78 FKK248 pKa = 10.41 STGNQQ253 pKa = 2.93
Molecular weight: 28.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.265
IPC2_protein 9.56
IPC_protein 9.736
Toseland 10.087
ProMoST 9.823
Dawson 10.306
Bjellqvist 10.014
Wikipedia 10.496
Rodwell 10.657
Grimsley 10.394
Solomon 10.335
Lehninger 10.292
Nozaki 10.116
DTASelect 9.999
Thurlkill 10.145
EMBOSS 10.496
Sillero 10.218
Patrickios 10.131
IPC_peptide 10.335
IPC2_peptide 8.902
IPC2.peptide.svr19 8.479
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1048
96
388
262.0
29.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.534 ± 0.424
2.29 ± 0.237
6.107 ± 0.943
4.676 ± 1.116
5.63 ± 0.532
6.202 ± 1.573
2.576 ± 0.604
4.962 ± 0.415
4.103 ± 0.901
6.775 ± 0.738
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.527 ± 0.17
5.153 ± 0.669
6.679 ± 0.904
4.771 ± 0.31
7.156 ± 0.284
7.156 ± 0.411
8.206 ± 0.75
4.389 ± 0.717
1.908 ± 0.103
4.198 ± 0.455
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here