Amino acid dipepetide frequency for Bacillus phage QCM8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.387AlaAla: 5.387 ± 0.399
0.318AlaCys: 0.318 ± 0.084
3.606AlaAsp: 3.606 ± 0.344
5.281AlaGlu: 5.281 ± 0.36
2.439AlaPhe: 2.439 ± 0.234
3.775AlaGly: 3.775 ± 0.466
1.23AlaHis: 1.23 ± 0.174
4.178AlaIle: 4.178 ± 0.334
5.196AlaLys: 5.196 ± 0.329
5.748AlaLeu: 5.748 ± 0.348
2.354AlaMet: 2.354 ± 0.223
3.16AlaAsn: 3.16 ± 0.392
2.46AlaPro: 2.46 ± 0.319
2.651AlaGln: 2.651 ± 0.309
2.524AlaArg: 2.524 ± 0.205
3.309AlaSer: 3.309 ± 0.327
4.433AlaThr: 4.433 ± 0.387
3.733AlaVal: 3.733 ± 0.262
0.933AlaTrp: 0.933 ± 0.13
2.566AlaTyr: 2.566 ± 0.206
0.0AlaXaa: 0.0 ± 0.0
Cys
0.382CysAla: 0.382 ± 0.08
0.127CysCys: 0.127 ± 0.053
0.339CysAsp: 0.339 ± 0.089
0.445CysGlu: 0.445 ± 0.105
0.361CysPhe: 0.361 ± 0.081
0.573CysGly: 0.573 ± 0.133
0.191CysHis: 0.191 ± 0.061
0.509CysIle: 0.509 ± 0.105
0.87CysLys: 0.87 ± 0.16
0.467CysLeu: 0.467 ± 0.115
0.382CysMet: 0.382 ± 0.091
0.594CysAsn: 0.594 ± 0.14
0.382CysPro: 0.382 ± 0.096
0.106CysGln: 0.106 ± 0.043
0.297CysArg: 0.297 ± 0.074
0.551CysSer: 0.551 ± 0.115
0.53CysThr: 0.53 ± 0.12
0.551CysVal: 0.551 ± 0.104
0.064CysTrp: 0.064 ± 0.039
0.424CysTyr: 0.424 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
3.627AspAla: 3.627 ± 0.277
0.573AspCys: 0.573 ± 0.111
3.16AspAsp: 3.16 ± 0.265
4.815AspGlu: 4.815 ± 0.331
2.672AspPhe: 2.672 ± 0.236
4.348AspGly: 4.348 ± 0.356
0.997AspHis: 0.997 ± 0.117
4.369AspIle: 4.369 ± 0.282
5.281AspLys: 5.281 ± 0.352
4.475AspLeu: 4.475 ± 0.298
1.633AspMet: 1.633 ± 0.22
2.927AspAsn: 2.927 ± 0.252
1.845AspPro: 1.845 ± 0.207
1.421AspGln: 1.421 ± 0.177
2.588AspArg: 2.588 ± 0.203
3.181AspSer: 3.181 ± 0.268
2.969AspThr: 2.969 ± 0.243
4.263AspVal: 4.263 ± 0.325
0.7AspTrp: 0.7 ± 0.132
2.948AspTyr: 2.948 ± 0.265
0.0AspXaa: 0.0 ± 0.0
Glu
4.221GluAla: 4.221 ± 0.334
0.806GluCys: 0.806 ± 0.134
4.984GluAsp: 4.984 ± 0.326
7.593GluGlu: 7.593 ± 0.514
3.033GluPhe: 3.033 ± 0.279
4.666GluGly: 4.666 ± 0.297
1.463GluHis: 1.463 ± 0.188
5.09GluIle: 5.09 ± 0.36
6.299GluLys: 6.299 ± 0.397
7.975GluLeu: 7.975 ± 0.403
2.439GluMet: 2.439 ± 0.22
3.5GluAsn: 3.5 ± 0.267
2.418GluPro: 2.418 ± 0.319
3.139GluGln: 3.139 ± 0.243
3.563GluArg: 3.563 ± 0.287
3.245GluSer: 3.245 ± 0.288
3.351GluThr: 3.351 ± 0.248
5.324GluVal: 5.324 ± 0.331
0.87GluTrp: 0.87 ± 0.129
3.203GluTyr: 3.203 ± 0.279
0.0GluXaa: 0.0 ± 0.0
Phe
2.206PheAla: 2.206 ± 0.21
0.424PheCys: 0.424 ± 0.104
2.736PheAsp: 2.736 ± 0.218
2.672PheGlu: 2.672 ± 0.224
1.273PhePhe: 1.273 ± 0.189
2.333PheGly: 2.333 ± 0.225
0.742PheHis: 0.742 ± 0.13
2.248PheIle: 2.248 ± 0.215
2.885PheLys: 2.885 ± 0.253
3.394PheLeu: 3.394 ± 0.279
1.251PheMet: 1.251 ± 0.164
2.142PheAsn: 2.142 ± 0.259
1.103PhePro: 1.103 ± 0.171
1.485PheGln: 1.485 ± 0.226
1.76PheArg: 1.76 ± 0.21
2.906PheSer: 2.906 ± 0.278
2.715PheThr: 2.715 ± 0.257
2.715PheVal: 2.715 ± 0.233
0.297PheTrp: 0.297 ± 0.081
1.718PheTyr: 1.718 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
4.433GlyAla: 4.433 ± 0.42
0.424GlyCys: 0.424 ± 0.094
3.097GlyAsp: 3.097 ± 0.284
4.178GlyGlu: 4.178 ± 0.279
2.757GlyPhe: 2.757 ± 0.286
5.515GlyGly: 5.515 ± 0.853
1.315GlyHis: 1.315 ± 0.147
3.5GlyIle: 3.5 ± 0.272
5.133GlyLys: 5.133 ± 0.301
4.603GlyLeu: 4.603 ± 0.336
2.163GlyMet: 2.163 ± 0.266
3.754GlyAsn: 3.754 ± 0.279
0.0GlyPro: 0.0 ± 0.0
2.651GlyGln: 2.651 ± 0.222
2.694GlyArg: 2.694 ± 0.235
4.539GlySer: 4.539 ± 0.443
5.302GlyThr: 5.302 ± 0.487
4.624GlyVal: 4.624 ± 0.349
0.87GlyTrp: 0.87 ± 0.153
2.927GlyTyr: 2.927 ± 0.224
0.0GlyXaa: 0.0 ± 0.0
His
0.976HisAla: 0.976 ± 0.157
0.17HisCys: 0.17 ± 0.056
1.103HisAsp: 1.103 ± 0.171
1.23HisGlu: 1.23 ± 0.151
0.573HisPhe: 0.573 ± 0.081
0.933HisGly: 0.933 ± 0.127
0.339HisHis: 0.339 ± 0.08
1.294HisIle: 1.294 ± 0.172
1.251HisLys: 1.251 ± 0.154
1.591HisLeu: 1.591 ± 0.202
0.509HisMet: 0.509 ± 0.097
1.06HisAsn: 1.06 ± 0.19
0.785HisPro: 0.785 ± 0.123
0.551HisGln: 0.551 ± 0.1
0.912HisArg: 0.912 ± 0.128
0.933HisSer: 0.933 ± 0.13
1.124HisThr: 1.124 ± 0.169
1.527HisVal: 1.527 ± 0.192
0.297HisTrp: 0.297 ± 0.078
0.806HisTyr: 0.806 ± 0.118
0.0HisXaa: 0.0 ± 0.0
Ile
4.03IleAla: 4.03 ± 0.283
0.509IleCys: 0.509 ± 0.101
4.496IleAsp: 4.496 ± 0.339
5.112IleGlu: 5.112 ± 0.345
2.1IlePhe: 2.1 ± 0.227
4.115IleGly: 4.115 ± 0.332
1.082IleHis: 1.082 ± 0.151
3.754IleIle: 3.754 ± 0.284
5.09IleLys: 5.09 ± 0.328
4.878IleLeu: 4.878 ± 0.422
1.909IleMet: 1.909 ± 0.251
3.5IleAsn: 3.5 ± 0.279
2.757IlePro: 2.757 ± 0.246
2.375IleGln: 2.375 ± 0.186
2.545IleArg: 2.545 ± 0.211
3.712IleSer: 3.712 ± 0.222
4.03IleThr: 4.03 ± 0.374
4.687IleVal: 4.687 ± 0.287
0.403IleTrp: 0.403 ± 0.113
2.482IleTyr: 2.482 ± 0.21
0.0IleXaa: 0.0 ± 0.0
Lys
5.069LysAla: 5.069 ± 0.311
0.488LysCys: 0.488 ± 0.118
4.581LysAsp: 4.581 ± 0.296
7.869LysGlu: 7.869 ± 0.469
2.948LysPhe: 2.948 ± 0.27
5.048LysGly: 5.048 ± 0.336
1.548LysHis: 1.548 ± 0.193
4.263LysIle: 4.263 ± 0.307
7.148LysLys: 7.148 ± 0.555
6.342LysLeu: 6.342 ± 0.369
2.778LysMet: 2.778 ± 0.274
3.627LysAsn: 3.627 ± 0.252
2.991LysPro: 2.991 ± 0.276
3.733LysGln: 3.733 ± 0.309
4.093LysArg: 4.093 ± 0.367
4.178LysSer: 4.178 ± 0.298
3.627LysThr: 3.627 ± 0.292
6.257LysVal: 6.257 ± 0.317
0.764LysTrp: 0.764 ± 0.124
3.097LysTyr: 3.097 ± 0.313
0.0LysXaa: 0.0 ± 0.0
Leu
6.002LeuAla: 6.002 ± 0.349
0.424LeuCys: 0.424 ± 0.104
5.324LeuAsp: 5.324 ± 0.323
6.236LeuGlu: 6.236 ± 0.375
3.266LeuPhe: 3.266 ± 0.256
4.772LeuGly: 4.772 ± 0.325
1.273LeuHis: 1.273 ± 0.161
5.239LeuIle: 5.239 ± 0.358
6.49LeuLys: 6.49 ± 0.354
5.684LeuLeu: 5.684 ± 0.314
2.079LeuMet: 2.079 ± 0.235
3.669LeuAsn: 3.669 ± 0.303
3.415LeuPro: 3.415 ± 0.289
3.351LeuGln: 3.351 ± 0.276
4.369LeuArg: 4.369 ± 0.367
5.133LeuSer: 5.133 ± 0.37
5.218LeuThr: 5.218 ± 0.33
5.196LeuVal: 5.196 ± 0.312
0.912LeuTrp: 0.912 ± 0.128
3.16LeuTyr: 3.16 ± 0.237
0.0LeuXaa: 0.0 ± 0.0
Met
2.206MetAla: 2.206 ± 0.241
0.212MetCys: 0.212 ± 0.073
1.485MetAsp: 1.485 ± 0.197
2.566MetGlu: 2.566 ± 0.264
1.336MetPhe: 1.336 ± 0.163
1.442MetGly: 1.442 ± 0.217
0.615MetHis: 0.615 ± 0.136
2.1MetIle: 2.1 ± 0.258
2.694MetLys: 2.694 ± 0.218
2.439MetLeu: 2.439 ± 0.261
0.7MetMet: 0.7 ± 0.116
1.676MetAsn: 1.676 ± 0.207
0.679MetPro: 0.679 ± 0.134
1.039MetGln: 1.039 ± 0.157
1.527MetArg: 1.527 ± 0.21
1.76MetSer: 1.76 ± 0.217
2.1MetThr: 2.1 ± 0.199
1.633MetVal: 1.633 ± 0.19
0.382MetTrp: 0.382 ± 0.082
1.23MetTyr: 1.23 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
3.288AsnAla: 3.288 ± 0.272
0.445AsnCys: 0.445 ± 0.112
2.206AsnAsp: 2.206 ± 0.198
3.097AsnGlu: 3.097 ± 0.275
1.463AsnPhe: 1.463 ± 0.19
4.475AsnGly: 4.475 ± 0.377
0.912AsnHis: 0.912 ± 0.148
3.394AsnIle: 3.394 ± 0.317
4.03AsnLys: 4.03 ± 0.319
3.945AsnLeu: 3.945 ± 0.27
1.845AsnMet: 1.845 ± 0.224
2.906AsnAsn: 2.906 ± 0.286
2.63AsnPro: 2.63 ± 0.352
1.782AsnGln: 1.782 ± 0.197
2.694AsnArg: 2.694 ± 0.291
2.757AsnSer: 2.757 ± 0.266
3.351AsnThr: 3.351 ± 0.279
3.712AsnVal: 3.712 ± 0.327
0.742AsnTrp: 0.742 ± 0.119
2.333AsnTyr: 2.333 ± 0.222
0.0AsnXaa: 0.0 ± 0.0
Pro
2.227ProAla: 2.227 ± 0.323
0.233ProCys: 0.233 ± 0.077
1.909ProAsp: 1.909 ± 0.242
2.524ProGlu: 2.524 ± 0.272
1.336ProPhe: 1.336 ± 0.164
1.845ProGly: 1.845 ± 0.266
0.87ProHis: 0.87 ± 0.126
2.227ProIle: 2.227 ± 0.223
2.715ProLys: 2.715 ± 0.242
2.651ProLeu: 2.651 ± 0.249
0.912ProMet: 0.912 ± 0.177
2.142ProAsn: 2.142 ± 0.225
1.103ProPro: 1.103 ± 0.211
1.612ProGln: 1.612 ± 0.25
1.4ProArg: 1.4 ± 0.154
1.739ProSer: 1.739 ± 0.213
2.821ProThr: 2.821 ± 0.305
2.163ProVal: 2.163 ± 0.233
0.297ProTrp: 0.297 ± 0.099
1.485ProTyr: 1.485 ± 0.225
0.0ProXaa: 0.0 ± 0.0
Gln
3.351GlnAla: 3.351 ± 0.31
0.361GlnCys: 0.361 ± 0.085
1.909GlnAsp: 1.909 ± 0.218
3.457GlnGlu: 3.457 ± 0.295
1.315GlnPhe: 1.315 ± 0.156
2.248GlnGly: 2.248 ± 0.222
0.615GlnHis: 0.615 ± 0.106
2.333GlnIle: 2.333 ± 0.225
2.672GlnLys: 2.672 ± 0.253
3.33GlnLeu: 3.33 ± 0.315
1.357GlnMet: 1.357 ± 0.192
1.654GlnAsn: 1.654 ± 0.181
1.485GlnPro: 1.485 ± 0.25
2.482GlnGln: 2.482 ± 0.334
1.739GlnArg: 1.739 ± 0.184
2.036GlnSer: 2.036 ± 0.207
2.206GlnThr: 2.206 ± 0.204
2.609GlnVal: 2.609 ± 0.255
0.382GlnTrp: 0.382 ± 0.089
1.421GlnTyr: 1.421 ± 0.178
0.0GlnXaa: 0.0 ± 0.0
Arg
2.524ArgAla: 2.524 ± 0.208
0.318ArgCys: 0.318 ± 0.086
2.588ArgAsp: 2.588 ± 0.282
3.478ArgGlu: 3.478 ± 0.289
1.994ArgPhe: 1.994 ± 0.2
2.842ArgGly: 2.842 ± 0.253
0.636ArgHis: 0.636 ± 0.123
3.097ArgIle: 3.097 ± 0.211
3.86ArgLys: 3.86 ± 0.342
3.839ArgLeu: 3.839 ± 0.276
1.591ArgMet: 1.591 ± 0.172
2.375ArgAsn: 2.375 ± 0.261
1.294ArgPro: 1.294 ± 0.155
1.633ArgGln: 1.633 ± 0.19
2.036ArgArg: 2.036 ± 0.221
2.651ArgSer: 2.651 ± 0.26
2.375ArgThr: 2.375 ± 0.242
3.372ArgVal: 3.372 ± 0.258
0.53ArgTrp: 0.53 ± 0.106
2.206ArgTyr: 2.206 ± 0.196
0.0ArgXaa: 0.0 ± 0.0
Ser
3.457SerAla: 3.457 ± 0.298
0.467SerCys: 0.467 ± 0.09
3.521SerAsp: 3.521 ± 0.252
3.457SerGlu: 3.457 ± 0.298
2.842SerPhe: 2.842 ± 0.275
4.136SerGly: 4.136 ± 0.372
0.764SerHis: 0.764 ± 0.122
4.369SerIle: 4.369 ± 0.325
4.327SerLys: 4.327 ± 0.344
4.475SerLeu: 4.475 ± 0.297
1.612SerMet: 1.612 ± 0.147
2.821SerAsn: 2.821 ± 0.318
1.676SerPro: 1.676 ± 0.211
1.676SerGln: 1.676 ± 0.164
2.269SerArg: 2.269 ± 0.228
3.733SerSer: 3.733 ± 0.401
3.584SerThr: 3.584 ± 0.311
4.009SerVal: 4.009 ± 0.266
0.954SerTrp: 0.954 ± 0.144
2.757SerTyr: 2.757 ± 0.243
0.0SerXaa: 0.0 ± 0.0
Thr
3.542ThrAla: 3.542 ± 0.473
0.445ThrCys: 0.445 ± 0.097
3.584ThrAsp: 3.584 ± 0.337
4.306ThrGlu: 4.306 ± 0.286
2.906ThrPhe: 2.906 ± 0.241
4.666ThrGly: 4.666 ± 0.408
1.018ThrHis: 1.018 ± 0.146
4.136ThrIle: 4.136 ± 0.328
4.666ThrLys: 4.666 ± 0.313
5.09ThrLeu: 5.09 ± 0.344
1.188ThrMet: 1.188 ± 0.16
3.097ThrAsn: 3.097 ± 0.29
2.863ThrPro: 2.863 ± 0.363
2.057ThrGln: 2.057 ± 0.218
2.566ThrArg: 2.566 ± 0.222
3.5ThrSer: 3.5 ± 0.286
3.754ThrThr: 3.754 ± 0.326
4.921ThrVal: 4.921 ± 0.32
0.573ThrTrp: 0.573 ± 0.135
3.012ThrTyr: 3.012 ± 0.277
0.0ThrXaa: 0.0 ± 0.0
Val
4.815ValAla: 4.815 ± 0.351
0.7ValCys: 0.7 ± 0.123
4.369ValAsp: 4.369 ± 0.289
5.324ValGlu: 5.324 ± 0.345
2.46ValPhe: 2.46 ± 0.253
3.606ValGly: 3.606 ± 0.328
1.145ValHis: 1.145 ± 0.142
4.178ValIle: 4.178 ± 0.253
5.663ValLys: 5.663 ± 0.367
5.515ValLeu: 5.515 ± 0.357
1.866ValMet: 1.866 ± 0.195
3.563ValAsn: 3.563 ± 0.269
2.948ValPro: 2.948 ± 0.278
2.821ValGln: 2.821 ± 0.224
3.16ValArg: 3.16 ± 0.282
3.775ValSer: 3.775 ± 0.311
4.921ValThr: 4.921 ± 0.346
4.603ValVal: 4.603 ± 0.28
0.827ValTrp: 0.827 ± 0.123
3.436ValTyr: 3.436 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
0.742TrpAla: 0.742 ± 0.153
0.17TrpCys: 0.17 ± 0.063
0.806TrpAsp: 0.806 ± 0.129
0.806TrpGlu: 0.806 ± 0.116
0.509TrpPhe: 0.509 ± 0.084
0.7TrpGly: 0.7 ± 0.125
0.297TrpHis: 0.297 ± 0.079
0.636TrpIle: 0.636 ± 0.124
0.721TrpLys: 0.721 ± 0.115
0.954TrpLeu: 0.954 ± 0.151
0.148TrpMet: 0.148 ± 0.052
0.87TrpAsn: 0.87 ± 0.154
0.0TrpPro: 0.0 ± 0.0
0.551TrpGln: 0.551 ± 0.104
0.424TrpArg: 0.424 ± 0.085
0.721TrpSer: 0.721 ± 0.116
0.658TrpThr: 0.658 ± 0.08
0.785TrpVal: 0.785 ± 0.14
0.17TrpTrp: 0.17 ± 0.058
0.806TrpTyr: 0.806 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.8TyrAla: 2.8 ± 0.219
0.53TyrCys: 0.53 ± 0.102
2.991TyrAsp: 2.991 ± 0.216
2.736TyrGlu: 2.736 ± 0.243
1.421TyrPhe: 1.421 ± 0.173
2.439TyrGly: 2.439 ± 0.217
0.912TyrHis: 0.912 ± 0.156
2.651TyrIle: 2.651 ± 0.275
3.457TyrLys: 3.457 ± 0.294
3.818TyrLeu: 3.818 ± 0.319
1.082TyrMet: 1.082 ± 0.142
2.991TyrAsn: 2.991 ± 0.276
1.379TyrPro: 1.379 ± 0.161
1.845TyrGln: 1.845 ± 0.207
2.079TyrArg: 2.079 ± 0.198
2.545TyrSer: 2.545 ± 0.223
2.821TyrThr: 2.821 ± 0.204
3.012TyrVal: 3.012 ± 0.248
0.509TyrTrp: 0.509 ± 0.105
1.697TyrTyr: 1.697 ± 0.185
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 273 proteins (47149 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski