Spider associated circular virus 2
Average proteome isoelectric point is 7.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BP77|A0A346BP77_9VIRU Putative capsid protein OS=Spider associated circular virus 2 OX=2293303 PE=4 SV=1
MM1 pKa = 7.9 PFNFQARR8 pKa = 11.84 YY9 pKa = 9.97 ALLTYY14 pKa = 8.97 AQCDD18 pKa = 3.43 ALDD21 pKa = 3.81 GFRR24 pKa = 11.84 VMDD27 pKa = 4.22 HH28 pKa = 6.83 LSGLGAEE35 pKa = 4.95 CIISRR40 pKa = 11.84 EE41 pKa = 3.84 VHH43 pKa = 5.97 ANGGIHH49 pKa = 6.31 LHH51 pKa = 6.32 CFVDD55 pKa = 4.63 FEE57 pKa = 4.87 RR58 pKa = 11.84 KK59 pKa = 8.77 FRR61 pKa = 11.84 SRR63 pKa = 11.84 RR64 pKa = 11.84 TDD66 pKa = 2.64 IFDD69 pKa = 3.14 VDD71 pKa = 3.53 GRR73 pKa = 11.84 HH74 pKa = 5.9 PNIVSSYY81 pKa = 7.57 GSPWGGYY88 pKa = 10.22 DD89 pKa = 3.69 YY90 pKa = 10.87 AIKK93 pKa = 10.81 DD94 pKa = 3.36 GDD96 pKa = 4.29 VICGGLEE103 pKa = 3.73 RR104 pKa = 11.84 PEE106 pKa = 4.01 EE107 pKa = 4.12 PRR109 pKa = 11.84 SKK111 pKa = 10.41 RR112 pKa = 11.84 VAKK115 pKa = 10.18 DD116 pKa = 2.9 WNAWTEE122 pKa = 3.76 ITNARR127 pKa = 11.84 DD128 pKa = 3.44 RR129 pKa = 11.84 SHH131 pKa = 6.61 FWEE134 pKa = 5.0 LVHH137 pKa = 8.01 HH138 pKa = 7.16 LDD140 pKa = 4.88 PKK142 pKa = 10.84 AAACNHH148 pKa = 5.45 GQLAKK153 pKa = 9.28 YY154 pKa = 10.38 AEE156 pKa = 4.04 WRR158 pKa = 11.84 FRR160 pKa = 11.84 DD161 pKa = 3.88 TPAKK165 pKa = 9.94 YY166 pKa = 9.4 EE167 pKa = 4.26 SPGGIEE173 pKa = 4.88 FIGGDD178 pKa = 3.37 VDD180 pKa = 6.43 GRR182 pKa = 11.84 DD183 pKa = 3.34 AWCDD187 pKa = 3.17 QSGIRR192 pKa = 11.84 SGEE195 pKa = 3.79 PLVEE199 pKa = 4.15 LKK201 pKa = 10.54 KK202 pKa = 10.98 ASNDD206 pKa = 3.4 DD207 pKa = 3.12 VKK209 pKa = 11.37 YY210 pKa = 10.88 AIFDD214 pKa = 4.73 DD215 pKa = 3.41 IRR217 pKa = 11.84 GGIKK221 pKa = 9.97 FFPAFKK227 pKa = 10.19 EE228 pKa = 4.03 WMGAQAYY235 pKa = 6.96 VTVKK239 pKa = 10.19 EE240 pKa = 4.63 LYY242 pKa = 10.09 RR243 pKa = 11.84 EE244 pKa = 3.85 PALIKK249 pKa = 9.55 WGKK252 pKa = 8.34 PSIWLANDD260 pKa = 3.26 DD261 pKa = 3.48 PRR263 pKa = 11.84 NYY265 pKa = 10.08 VEE267 pKa = 4.09 QSDD270 pKa = 4.08 RR271 pKa = 11.84 SWLEE275 pKa = 3.69 EE276 pKa = 3.51 NCTFIEE282 pKa = 4.07 VTEE285 pKa = 4.81 AIFRR289 pKa = 11.84 ANTEE293 pKa = 3.84
Molecular weight: 33.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.259
IPC2_protein 5.283
IPC_protein 5.258
Toseland 5.372
ProMoST 5.385
Dawson 5.347
Bjellqvist 5.448
Wikipedia 5.27
Rodwell 5.283
Grimsley 5.372
Solomon 5.347
Lehninger 5.321
Nozaki 5.525
DTASelect 5.703
Thurlkill 5.461
EMBOSS 5.436
Sillero 5.601
Patrickios 4.05
IPC_peptide 5.359
IPC2_peptide 5.601
IPC2.peptide.svr19 5.569
Protein with the highest isoelectric point:
>tr|A0A346BP77|A0A346BP77_9VIRU Putative capsid protein OS=Spider associated circular virus 2 OX=2293303 PE=4 SV=1
MM1 pKa = 7.82 AYY3 pKa = 10.28 ARR5 pKa = 11.84 RR6 pKa = 11.84 NRR8 pKa = 11.84 YY9 pKa = 8.67 RR10 pKa = 11.84 RR11 pKa = 11.84 PTSRR15 pKa = 11.84 FAKK18 pKa = 10.04 KK19 pKa = 9.8 PYY21 pKa = 9.81 GGSKK25 pKa = 9.54 SRR27 pKa = 11.84 RR28 pKa = 11.84 SFVKK32 pKa = 9.78 RR33 pKa = 11.84 RR34 pKa = 11.84 TFKK37 pKa = 10.5 KK38 pKa = 9.92 RR39 pKa = 11.84 RR40 pKa = 11.84 PTMTSKK46 pKa = 11.08 RR47 pKa = 11.84 ILNLTATKK55 pKa = 10.5 KK56 pKa = 10.2 RR57 pKa = 11.84 DD58 pKa = 3.32 TMLTYY63 pKa = 10.89 TNVSSDD69 pKa = 3.26 AANGGTTYY77 pKa = 9.64 RR78 pKa = 11.84 TDD80 pKa = 3.11 VPAVLTGGQDD90 pKa = 3.62 VNSVRR95 pKa = 11.84 ALLWCATARR104 pKa = 11.84 DD105 pKa = 4.09 NTISSANNRR114 pKa = 11.84 GTAFDD119 pKa = 3.49 QAARR123 pKa = 11.84 TSTTPYY129 pKa = 10.13 MIGLKK134 pKa = 9.77 EE135 pKa = 4.24 AIEE138 pKa = 4.29 IQVSTGMPWQWRR150 pKa = 11.84 RR151 pKa = 11.84 ICFTYY156 pKa = 10.13 KK157 pKa = 10.83 GPIGLALGTSQNISTEE173 pKa = 4.1 TSNGWVRR180 pKa = 11.84 LVNDD184 pKa = 3.1 ITGNPGGGQQYY195 pKa = 11.02 ILFEE199 pKa = 4.4 KK200 pKa = 10.4 LFKK203 pKa = 10.71 GQNTQDD209 pKa = 3.02 WNDD212 pKa = 3.03 AMTAKK217 pKa = 10.2 VDD219 pKa = 3.61 NTRR222 pKa = 11.84 VTLKK226 pKa = 10.5 YY227 pKa = 11.01 DD228 pKa = 3.52 KK229 pKa = 10.49 TCTLASGNEE238 pKa = 3.83 DD239 pKa = 3.36 GFIRR243 pKa = 11.84 KK244 pKa = 8.39 YY245 pKa = 10.98 NKK247 pKa = 7.46 WHH249 pKa = 6.95 PMRR252 pKa = 11.84 KK253 pKa = 7.02 TLVYY257 pKa = 10.82 NDD259 pKa = 4.57 DD260 pKa = 3.74 EE261 pKa = 5.53 LGGGEE266 pKa = 3.97 AAQVFSTQGKK276 pKa = 10.1 AGMGDD281 pKa = 4.27 YY282 pKa = 10.87 YY283 pKa = 11.67 VLDD286 pKa = 4.14 LFRR289 pKa = 11.84 ARR291 pKa = 11.84 TGSTSSDD298 pKa = 3.1 QLSFRR303 pKa = 11.84 PEE305 pKa = 3.68 STLYY309 pKa = 9.33 WHH311 pKa = 7.11 EE312 pKa = 4.13 KK313 pKa = 9.32
Molecular weight: 35.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.252
IPC2_protein 9.545
IPC_protein 9.794
Toseland 10.189
ProMoST 9.911
Dawson 10.379
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 10.745
Grimsley 10.452
Solomon 10.423
Lehninger 10.379
Nozaki 10.189
DTASelect 10.058
Thurlkill 10.233
EMBOSS 10.599
Sillero 10.306
Patrickios 10.335
IPC_peptide 10.409
IPC2_peptide 8.814
IPC2.peptide.svr19 8.597
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
606
293
313
303.0
34.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.086 ± 0.533
1.65 ± 0.5
6.931 ± 1.084
5.116 ± 1.619
4.455 ± 0.68
8.416 ± 0.152
1.815 ± 0.85
4.785 ± 0.919
5.941 ± 0.556
5.941 ± 0.325
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.815 ± 0.535
4.785 ± 0.698
3.795 ± 0.434
2.97 ± 0.624
8.251 ± 0.502
6.106 ± 0.667
7.426 ± 2.946
4.785 ± 0.226
2.805 ± 0.411
4.125 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here