Avian infectious bronchitis virus (strain Beaudette) (IBV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Gammacoronavirus; Igacovirus; Avian coronavirus; Infectious bronchitis virus

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P69596|NCAP_IBVB Nucleoprotein OS=Avian infectious bronchitis virus (strain Beaudette) OX=11122 GN=N PE=1 SV=1
MM1 pKa = 7.59LNLEE5 pKa = 4.57VIIEE9 pKa = 4.17TGEE12 pKa = 3.84QVIQKK17 pKa = 10.01ISFNLQHH24 pKa = 6.73ISSVLNTEE32 pKa = 3.84VFDD35 pKa = 4.52PFDD38 pKa = 3.29YY39 pKa = 10.68CYY41 pKa = 11.2YY42 pKa = 10.31RR43 pKa = 11.84GGNFWEE49 pKa = 4.43IEE51 pKa = 4.21SAEE54 pKa = 4.26DD55 pKa = 4.39CSGDD59 pKa = 4.48DD60 pKa = 4.1EE61 pKa = 5.77FIEE64 pKa = 4.42

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q89786|NS5B_IBVB Non-structural protein 5b OS=Avian infectious bronchitis virus (strain Beaudette) OX=11122 GN=5b PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.77WLTSFGRR9 pKa = 11.84AVISCYY15 pKa = 10.41KK16 pKa = 10.54SLLLTQLRR24 pKa = 11.84VLDD27 pKa = 4.68RR28 pKa = 11.84LILDD32 pKa = 3.73HH33 pKa = 7.01GLLRR37 pKa = 11.84VLTCSRR43 pKa = 11.84RR44 pKa = 11.84VLLVQLDD51 pKa = 3.83LVYY54 pKa = 10.88RR55 pKa = 11.84LAYY58 pKa = 9.62TPTQSLAA65 pKa = 3.29

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

12752

57

6629

1275.2

142.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.775 ± 0.259

3.286 ± 0.16

5.536 ± 0.502

4.705 ± 0.359

5.56 ± 0.234

6.156 ± 0.477

1.772 ± 0.26

5.301 ± 0.384

6.43 ± 0.536

8.752 ± 1.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.733 ± 0.134

5.254 ± 0.518

3.756 ± 0.375

3.56 ± 0.593

3.294 ± 0.582

6.611 ± 0.503

5.874 ± 0.279

9.622 ± 1.07

1.38 ± 0.221

4.642 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski