Pacificimonas flava

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingosinicellaceae; Pacificimonas

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3056 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M2SCL7|M2SCL7_9SPHN Potassium efflux system KefA protein / Small-conductance mechanosensitive channel OS=Pacificimonas flava OX=1234595 GN=C725_1718 PE=3 SV=1
MM1 pKa = 7.69SYY3 pKa = 9.56TRR5 pKa = 11.84ALLLVGSALALASCGADD22 pKa = 3.12DD23 pKa = 3.9VASPGGGVIVVPAPTPAPGPSPSPSPTPTPTPTPTPTGGAAEE65 pKa = 4.54DD66 pKa = 4.68CPVGTALTNQILDD79 pKa = 3.61YY80 pKa = 9.48GTQTEE85 pKa = 4.38EE86 pKa = 4.75DD87 pKa = 3.99DD88 pKa = 5.61ALICQLSGTISEE100 pKa = 4.87DD101 pKa = 3.15LTLEE105 pKa = 4.17ALPGVVYY112 pKa = 10.69SLNGRR117 pKa = 11.84VEE119 pKa = 4.13VGTDD123 pKa = 2.78AGGATEE129 pKa = 4.55PRR131 pKa = 11.84ANSQQANLTIEE142 pKa = 4.39AGVVMFGSSGADD154 pKa = 2.87FLLINRR160 pKa = 11.84GSQLNVQGTEE170 pKa = 3.86DD171 pKa = 4.12RR172 pKa = 11.84PVIMTSRR179 pKa = 11.84TNMTGEE185 pKa = 3.78VDD187 pKa = 3.8EE188 pKa = 5.02NSIGQWGGLVILGRR202 pKa = 11.84APISDD207 pKa = 3.9CAGDD211 pKa = 3.88AEE213 pKa = 4.48QGGTATCEE221 pKa = 3.83AFVEE225 pKa = 5.08GVSQASYY232 pKa = 10.45GGEE235 pKa = 3.94VADD238 pKa = 5.27DD239 pKa = 3.71NSGTIQYY246 pKa = 9.87LQVRR250 pKa = 11.84YY251 pKa = 9.1PGFEE255 pKa = 3.79VTEE258 pKa = 4.31GNEE261 pKa = 4.02LNGITMGGVGTGTTFDD277 pKa = 4.82HH278 pKa = 6.13IQVHH282 pKa = 5.79NSSDD286 pKa = 3.41DD287 pKa = 4.28GIEE290 pKa = 4.16HH291 pKa = 7.51FGGTVNARR299 pKa = 11.84YY300 pKa = 9.15VALTGNDD307 pKa = 3.59DD308 pKa = 4.77DD309 pKa = 6.75SFDD312 pKa = 3.68TDD314 pKa = 3.37LGYY317 pKa = 10.99KK318 pKa = 10.14GFYY321 pKa = 8.94QFVVITQRR329 pKa = 11.84EE330 pKa = 4.1LGADD334 pKa = 3.99RR335 pKa = 11.84LWEE338 pKa = 4.41ADD340 pKa = 3.51SEE342 pKa = 4.63GNEE345 pKa = 4.09NAIPRR350 pKa = 11.84QDD352 pKa = 3.25VRR354 pKa = 11.84LVNATMLGQSGSNQILFRR372 pKa = 11.84GGGDD376 pKa = 3.29YY377 pKa = 10.93AIYY380 pKa = 10.72NSVLASPEE388 pKa = 3.88NEE390 pKa = 3.74QCLDD394 pKa = 2.99IDD396 pKa = 4.34GAQTVAAANPDD407 pKa = 3.65ADD409 pKa = 3.82EE410 pKa = 4.39VGPPRR415 pKa = 11.84FGSFVVDD422 pKa = 3.85CANPFVADD430 pKa = 3.86EE431 pKa = 4.84DD432 pKa = 5.14GIDD435 pKa = 3.32NAQYY439 pKa = 10.51FVGEE443 pKa = 4.16NVDD446 pKa = 3.44TDD448 pKa = 3.87FSNTLGGDD456 pKa = 3.29SGLVNGANEE465 pKa = 4.11TAVVVTDD472 pKa = 3.56VTSLSDD478 pKa = 3.73FLMATDD484 pKa = 4.15YY485 pKa = 10.95IGAVAEE491 pKa = 5.46GEE493 pKa = 4.6TPWFNGWTCDD503 pKa = 3.24IGGADD508 pKa = 3.42TCIARR513 pKa = 11.84PQTRR517 pKa = 11.84VAQQ520 pKa = 3.82

Molecular weight: 53.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M2T8V2|M2T8V2_9SPHN Histidine kinase OS=Pacificimonas flava OX=1234595 GN=C725_1527 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATQSGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84KK40 pKa = 8.79KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3056

0

3056

965605

37

1587

316.0

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.106 ± 0.077

0.732 ± 0.013

6.221 ± 0.038

6.169 ± 0.045

3.559 ± 0.029

9.038 ± 0.042

1.88 ± 0.022

4.85 ± 0.032

2.74 ± 0.038

9.845 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.021

2.458 ± 0.027

5.241 ± 0.034

3.052 ± 0.025

7.592 ± 0.047

5.382 ± 0.028

5.172 ± 0.027

6.931 ± 0.039

1.311 ± 0.02

2.235 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski