Beihai narna-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIF2|A0A1L3KIF2_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 8 OX=1922460 PE=4 SV=1
MM1 pKa = 7.85PEE3 pKa = 3.89RR4 pKa = 11.84VSRR7 pKa = 11.84TDD9 pKa = 3.48LGPILTRR16 pKa = 11.84FGIDD20 pKa = 3.46FQSKK24 pKa = 10.06SSHH27 pKa = 6.18LLLLFSRR34 pKa = 11.84EE35 pKa = 3.75EE36 pKa = 3.58RR37 pKa = 11.84DD38 pKa = 3.45YY39 pKa = 11.86AFSAFAFLRR48 pKa = 11.84SLTEE52 pKa = 4.02CLSDD56 pKa = 3.39ADD58 pKa = 5.01RR59 pKa = 11.84VLEE62 pKa = 4.59CPDD65 pKa = 3.3VLDD68 pKa = 4.85FVPTYY73 pKa = 9.68RR74 pKa = 11.84WLMDD78 pKa = 3.06VHH80 pKa = 8.14SGEE83 pKa = 4.31EE84 pKa = 3.71QVKK87 pKa = 10.12RR88 pKa = 11.84MKK90 pKa = 10.62FLTAWPMARR99 pKa = 11.84WLRR102 pKa = 11.84NPLPPPPPRR111 pKa = 11.84VDD113 pKa = 3.14SDD115 pKa = 3.5HH116 pKa = 6.71FRR118 pKa = 11.84LPFAGRR124 pKa = 11.84THH126 pKa = 6.27RR127 pKa = 11.84HH128 pKa = 5.18LRR130 pKa = 11.84NLLASRR136 pKa = 11.84TTSVRR141 pKa = 11.84SCTVFVALLQGVKK154 pKa = 10.24RR155 pKa = 11.84GCAEE159 pKa = 3.8VNEE162 pKa = 4.43EE163 pKa = 4.42FISAALAKK171 pKa = 9.76HH172 pKa = 4.67QQALRR177 pKa = 11.84TPIPALDD184 pKa = 4.32PDD186 pKa = 3.59TRR188 pKa = 11.84RR189 pKa = 11.84NVRR192 pKa = 11.84AKK194 pKa = 10.6FSALWRR200 pKa = 11.84RR201 pKa = 11.84EE202 pKa = 4.22TPSRR206 pKa = 11.84QYY208 pKa = 11.59GEE210 pKa = 3.77WKK212 pKa = 9.92KK213 pKa = 11.0VYY215 pKa = 10.09NRR217 pKa = 11.84EE218 pKa = 4.03GKK220 pKa = 10.08CRR222 pKa = 11.84IARR225 pKa = 11.84RR226 pKa = 11.84EE227 pKa = 4.1HH228 pKa = 5.61NCSIKK233 pKa = 10.82ASFGYY238 pKa = 10.22SRR240 pKa = 11.84GAGGRR245 pKa = 11.84VGALARR251 pKa = 11.84LAKK254 pKa = 10.33DD255 pKa = 3.41YY256 pKa = 7.62WQCGHH261 pKa = 6.56NEE263 pKa = 4.45LLSMDD268 pKa = 4.41YY269 pKa = 10.66DD270 pKa = 3.88PSSGRR275 pKa = 11.84VVTQYY280 pKa = 11.09GIPSLGYY287 pKa = 9.62AALGKK292 pKa = 9.74LALMEE297 pKa = 4.18VSDD300 pKa = 5.06PQGQCHH306 pKa = 5.7ATDD309 pKa = 3.38ALLYY313 pKa = 7.42RR314 pKa = 11.84TPEE317 pKa = 4.16LLRR320 pKa = 11.84GDD322 pKa = 4.62GLCSADD328 pKa = 3.47VKK330 pKa = 10.99PILEE334 pKa = 4.16PLKK337 pKa = 10.84CRR339 pKa = 11.84LITAGSPVSYY349 pKa = 9.45AYY351 pKa = 11.11SMSAQKK357 pKa = 11.11SMWRR361 pKa = 11.84HH362 pKa = 4.56LHH364 pKa = 7.35DD365 pKa = 4.71NFPQFKK371 pKa = 10.51LIGSPLVEE379 pKa = 6.09DD380 pKa = 3.75DD381 pKa = 4.47LGWVRR386 pKa = 11.84GQTSSLDD393 pKa = 3.52LPFDD397 pKa = 3.26QWVSGDD403 pKa = 3.54YY404 pKa = 10.74SAATDD409 pKa = 3.93GLGSEE414 pKa = 4.64INRR417 pKa = 11.84LCIDD421 pKa = 4.43AYY423 pKa = 10.96CDD425 pKa = 3.38AVQATPTEE433 pKa = 4.19RR434 pKa = 11.84KK435 pKa = 8.14IWRR438 pKa = 11.84AVSGNHH444 pKa = 6.28SIHH447 pKa = 5.26YY448 pKa = 8.71TGEE451 pKa = 4.04KK452 pKa = 9.85VADD455 pKa = 3.55AVTASVGSSDD465 pKa = 3.88PFLMEE470 pKa = 4.38NGQLMGSVLSFPLLCAINLVAYY492 pKa = 7.74WAALEE497 pKa = 4.25EE498 pKa = 4.34FTGRR502 pKa = 11.84RR503 pKa = 11.84FQPNDD508 pKa = 3.27LPVLVNGDD516 pKa = 4.72DD517 pKa = 3.61ICFLANDD524 pKa = 4.03AFYY527 pKa = 10.87KK528 pKa = 10.26VWQDD532 pKa = 3.12WISRR536 pKa = 11.84VGFTLSLGKK545 pKa = 10.39NYY547 pKa = 9.84ISPDD551 pKa = 3.15FVTINSEE558 pKa = 3.9SFMYY562 pKa = 9.66SQRR565 pKa = 11.84RR566 pKa = 11.84CTFRR570 pKa = 11.84RR571 pKa = 11.84IGFLNTGLLIPSASIRR587 pKa = 11.84PEE589 pKa = 3.64NRR591 pKa = 11.84RR592 pKa = 11.84MPLTGKK598 pKa = 9.36LQRR601 pKa = 11.84LLSEE605 pKa = 4.59CNNPEE610 pKa = 3.87RR611 pKa = 11.84TWRR614 pKa = 11.84RR615 pKa = 11.84FIHH618 pKa = 5.56YY619 pKa = 9.67NKK621 pKa = 10.36AALRR625 pKa = 11.84DD626 pKa = 3.59LTLNGNVNVFGANALGSLGVTPPPGLEE653 pKa = 3.95PTYY656 pKa = 10.0TAYY659 pKa = 9.6QKK661 pKa = 11.4KK662 pKa = 7.76MAKK665 pKa = 9.63FLYY668 pKa = 9.87DD669 pKa = 3.09RR670 pKa = 11.84HH671 pKa = 5.88LATWADD677 pKa = 3.51NEE679 pKa = 4.18EE680 pKa = 4.24AKK682 pKa = 10.94VGSVWKK688 pKa = 10.18NVPPKK693 pKa = 8.98EE694 pKa = 4.09HH695 pKa = 7.25KK696 pKa = 10.95LPGSYY701 pKa = 10.57LLEE704 pKa = 4.8GCDD707 pKa = 4.92DD708 pKa = 3.69ATILAKK714 pKa = 10.26KK715 pKa = 9.4KK716 pKa = 9.76HH717 pKa = 6.27LGTEE721 pKa = 3.53RR722 pKa = 11.84CYY724 pKa = 10.5PVGLVHH730 pKa = 6.64EE731 pKa = 5.06SEE733 pKa = 3.81WSEE736 pKa = 4.14EE737 pKa = 4.14NEE739 pKa = 4.32LPLPAALPALNYY751 pKa = 10.23QLFGGPLTYY760 pKa = 10.59FGDD763 pKa = 3.99VSWSVKK769 pKa = 10.2YY770 pKa = 10.65LSKK773 pKa = 11.13ADD775 pKa = 3.57LRR777 pKa = 11.84SFRR780 pKa = 11.84SCLRR784 pKa = 11.84RR785 pKa = 11.84AEE787 pKa = 4.08RR788 pKa = 11.84DD789 pKa = 3.28PRR791 pKa = 11.84EE792 pKa = 3.57WKK794 pKa = 10.15YY795 pKa = 11.28RR796 pKa = 11.84LGRR799 pKa = 11.84MSTDD803 pKa = 3.06EE804 pKa = 4.11TPATNKK810 pKa = 9.92LRR812 pKa = 11.84VEE814 pKa = 4.66MPTEE818 pKa = 4.11PPPTGVEE825 pKa = 4.44GPADD829 pKa = 3.71SVRR832 pKa = 11.84RR833 pKa = 11.84WW834 pKa = 3.0

Molecular weight: 94.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIF2|A0A1L3KIF2_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 8 OX=1922460 PE=4 SV=1
MM1 pKa = 7.62AANNKK6 pKa = 7.67KK7 pKa = 7.14TTVTTTTTQSNGASIPRR24 pKa = 11.84RR25 pKa = 11.84WGRR28 pKa = 11.84AARR31 pKa = 11.84RR32 pKa = 11.84PLEE35 pKa = 4.4GIKK38 pKa = 10.21QGVGQVTIEE47 pKa = 4.08AFPRR51 pKa = 11.84AARR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84PRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84TGNPMRR66 pKa = 11.84GYY68 pKa = 10.78FNALSPHH75 pKa = 6.2HH76 pKa = 6.7LALPRR81 pKa = 11.84ATGPYY86 pKa = 8.14MVCRR90 pKa = 11.84TTTRR94 pKa = 11.84FSSTGVVHH102 pKa = 7.13LLGPMTAIPVAQTEE116 pKa = 4.74SPWAAVVGVTHH127 pKa = 7.2PGGGHH132 pKa = 4.94VNPMNGINNVIPIIDD147 pKa = 3.84AGLNVSGFNDD157 pKa = 3.52SRR159 pKa = 11.84VTPAAFTVQVMNPGALQTTSGIVYY183 pKa = 9.58QGRR186 pKa = 11.84VNNVPALMDD195 pKa = 5.2DD196 pKa = 3.49PRR198 pKa = 11.84TWAAYY203 pKa = 10.09ADD205 pKa = 4.08SLVSFCSPRR214 pKa = 11.84MCSAGKK220 pKa = 9.56LALRR224 pKa = 11.84GTQTSLVPFNMSKK237 pKa = 10.46LAEE240 pKa = 4.07FSEE243 pKa = 4.84RR244 pKa = 11.84SLVVPGATTWNPGTLGLQFQGFAPMFVYY272 pKa = 10.66NPDD275 pKa = 4.2GIEE278 pKa = 3.91LEE280 pKa = 4.37YY281 pKa = 11.05VVTIEE286 pKa = 3.44WRR288 pKa = 11.84VRR290 pKa = 11.84FDD292 pKa = 3.66PSNPAHH298 pKa = 6.8AGSEE302 pKa = 4.12YY303 pKa = 10.45HH304 pKa = 6.75PPSSDD309 pKa = 3.68SLWSRR314 pKa = 11.84CVEE317 pKa = 4.02AASSVGNGVEE327 pKa = 4.82DD328 pKa = 3.56IADD331 pKa = 3.42VVAAVGAAAALLL343 pKa = 4.02

Molecular weight: 36.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1177

343

834

588.5

65.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.921 ± 1.276

1.954 ± 0.41

4.758 ± 0.96

4.843 ± 0.852

3.908 ± 0.213

7.392 ± 0.858

2.124 ± 0.043

3.059 ± 0.229

3.483 ± 1.055

9.686 ± 1.856

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.412

4.248 ± 0.673

7.052 ± 0.579

2.719 ± 0.102

8.071 ± 0.408

7.562 ± 0.142

5.947 ± 1.154

6.882 ± 1.427

2.209 ± 0.239

3.059 ± 0.53
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski