Morganella phage IME1369_01
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6JP04|A0A1W6JP04_9CAUD Uncharacterized protein OS=Morganella phage IME1369_01 OX=1965532 PE=4 SV=1
MM1 pKa = 7.55 TEE3 pKa = 3.84 DD4 pKa = 4.88 CIVAGLNTPLNDD16 pKa = 3.31 NDD18 pKa = 4.14 VITIVPVIGGAKK30 pKa = 9.89 SGGFLGIIGGAALIAGAFFIPGGFLATMTSTAMFAAGVGIAAAGVATMLTRR81 pKa = 11.84 TPGAPNFSEE90 pKa = 4.84 GNSEE94 pKa = 4.14 SNQYY98 pKa = 10.1 FSSLSNRR105 pKa = 11.84 IGQGYY110 pKa = 7.38 PVPLCYY116 pKa = 10.89 GEE118 pKa = 4.32 MVVGSNVISQGLEE131 pKa = 4.05 TVV133 pKa = 3.4
Molecular weight: 13.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.938
IPC2_protein 4.228
IPC_protein 3.986
Toseland 3.834
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.834
Grimsley 3.757
Solomon 3.923
Lehninger 3.884
Nozaki 4.101
DTASelect 4.19
Thurlkill 3.884
EMBOSS 3.884
Sillero 4.101
Patrickios 0.604
IPC_peptide 3.935
IPC2_peptide 4.088
IPC2.peptide.svr19 4.02
Protein with the highest isoelectric point:
>tr|A0A1W6JNU1|A0A1W6JNU1_9CAUD Uncharacterized protein OS=Morganella phage IME1369_01 OX=1965532 PE=4 SV=1
MM1 pKa = 7.99 PKK3 pKa = 10.06 PKK5 pKa = 10.19 NNRR8 pKa = 11.84 VRR10 pKa = 11.84 WLEE13 pKa = 3.55 PRR15 pKa = 11.84 EE16 pKa = 4.12 AKK18 pKa = 10.41 RR19 pKa = 11.84 LIDD22 pKa = 3.75 EE23 pKa = 4.61 CSEE26 pKa = 3.98 PLKK29 pKa = 11.05 SVVTFALATGLRR41 pKa = 11.84 RR42 pKa = 11.84 SNIINLEE49 pKa = 3.78 WSQIDD54 pKa = 3.79 MPRR57 pKa = 11.84 KK58 pKa = 9.09 VAWIPPDD65 pKa = 3.27 QTKK68 pKa = 10.43 SGKK71 pKa = 10.2 ALGVALNDD79 pKa = 3.54 TACRR83 pKa = 11.84 VLRR86 pKa = 11.84 EE87 pKa = 4.01 QIGNHH92 pKa = 4.01 TKK94 pKa = 10.55 YY95 pKa = 11.08 VFVHH99 pKa = 6.2 TKK101 pKa = 9.89 AKK103 pKa = 9.99 NRR105 pKa = 11.84 SDD107 pKa = 3.67 GSMTPVVRR115 pKa = 11.84 KK116 pKa = 8.95 MRR118 pKa = 11.84 VDD120 pKa = 3.03 GNTAFRR126 pKa = 11.84 TALKK130 pKa = 10.39 RR131 pKa = 11.84 AGIEE135 pKa = 3.56 NFRR138 pKa = 11.84 FHH140 pKa = 7.69 DD141 pKa = 4.82 LRR143 pKa = 11.84 HH144 pKa = 5.7 TWASWLIQAGVPLSVLQKK162 pKa = 10.71 KK163 pKa = 8.23 WGGWEE168 pKa = 4.13 SIEE171 pKa = 3.96 MVQRR175 pKa = 11.84 YY176 pKa = 7.75 AHH178 pKa = 7.27 LAPNHH183 pKa = 5.53 LVEE186 pKa = 4.02 HH187 pKa = 6.85 ARR189 pKa = 11.84 RR190 pKa = 11.84 IDD192 pKa = 3.32 EE193 pKa = 4.23 AMGINGTNMARR204 pKa = 11.84 ALIKK208 pKa = 10.58 AVV210 pKa = 3.24
Molecular weight: 23.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.516
IPC_protein 9.823
Toseland 10.76
ProMoST 10.306
Dawson 10.818
Bjellqvist 10.452
Wikipedia 10.965
Rodwell 11.169
Grimsley 10.833
Solomon 10.906
Lehninger 10.891
Nozaki 10.73
DTASelect 10.438
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.745
Patrickios 10.906
IPC_peptide 10.921
IPC2_peptide 9.107
IPC2.peptide.svr19 8.735
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
67
0
67
12802
38
1107
191.1
21.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.467 ± 0.801
1.226 ± 0.162
5.726 ± 0.309
6.452 ± 0.295
3.898 ± 0.3
7.1 ± 0.364
1.742 ± 0.166
6.421 ± 0.331
6.554 ± 0.312
7.397 ± 0.343
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.773 ± 0.155
4.968 ± 0.243
3.656 ± 0.291
4.335 ± 0.33
5.726 ± 0.32
6.491 ± 0.185
5.671 ± 0.286
6.616 ± 0.242
1.508 ± 0.14
3.273 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here